LOCUS       NM_001419292            1520 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus lipid droplet assembly factor 1 (Ldaf1), transcript
            variant 4, mRNA.
ACCESSION   NM_001419292 XM_030242508
VERSION     NM_001419292.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Yu,S., Viswakarma,N., Batra,S.K., Sambasiva Rao,M. and Reddy,J.K.
  TITLE     Identification of promethin and PGLP as two novel up-regulated
            genes in PPARgamma1-induced adipogenic mouse liver
  JOURNAL   Biochimie 86 (11), 743-761 (2004)
   PUBMED   15589683
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122853.3.
            
            On Mar 30, 2023 this sequence version replaced XM_030242508.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.3822178.1,
                                           SRR13422600.1835744.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849379, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-140               AC122853.3         118795-118934
            141-196             AC122853.3         119320-119375
            197-396             AC122853.3         121049-121248
            397-565             AC122853.3         131805-131973
            566-704             AC122853.3         132639-132777
            705-1520            AC122853.3         136593-137408
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 64.37 cM"
     gene            1..1520
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /note="lipid droplet assembly factor 1"
                     /db_xref="GeneID:233806"
                     /db_xref="MGI:MGI:1925752"
     exon            1..140
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /inference="alignment:Splign:2.1.0"
     exon            141..196
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /inference="alignment:Splign:2.1.0"
     exon            197..396
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /inference="alignment:Splign:2.1.0"
     CDS             301..786
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /note="isoform 1 is encoded by transcript variant 4;
                     promethin; transmembrane protein 159"
                     /codon_start=1
                     /product="lipid droplet assembly factor 1 isoform 1"
                     /protein_id="NP_001406221.1"
                     /db_xref="GeneID:233806"
                     /db_xref="MGI:MGI:1925752"
                     /translation="MAKEEPPSVSRDLQELQRKLGLLLESFLNNSKVVAFMKSPVGRF
                     LDRHPFVTLTVLMFVTMSAIPVGFFLLIVVLTSLGALMGAILLEGLVISVCGLSLLCV
                     LCGLGFLSLALSGIAMMSYVVVSCLMSYWFSPSRPLTQQNANVDCQLAMKFTESERLI
                     F"
     exon            397..565
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /inference="alignment:Splign:2.1.0"
     exon            566..704
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /inference="alignment:Splign:2.1.0"
     exon            705..1520
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1413..1418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /note="hexamer: AATAAA"
     regulatory      1434..1439
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /note="hexamer: AAGAAA"
     polyA_site      1444
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
     polyA_site      1453
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /note="major polyA site"
     regulatory      1499..1504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
                     /note="hexamer: ATTAAA"
     polyA_site      1520
                     /gene="Ldaf1"
                     /gene_synonym="8430420C20Rik; Tmem159"
ORIGIN      
        1 agaggaagag cgcttgctgg tgaggaggag gaggtccagc tttcacccct aatcaggcct
       61 gcgtcccttc ttgtcttcct tccacttcgc tttgcgccct ctgtggctgc agggcggggt
      121 ttcgcggcag ccagcaccag gattggtcaa caagggtgct attcgccaca gcaggaggga
      181 gctacaggtg tcaaagcgat agcaagagac ggggggacat gagagattgg gctacgtact
      241 acagtaatgc atttcctggt agagagttta ctctagagtg agactgacag acatttcaag
      301 atggccaaag aagagccccc gagtgtctcc agagacttac aggagctgca aaggaagctg
      361 ggtttgctgt tagagtcttt cctgaataac tcaaaggtgg ttgcctttat gaagtccccg
      421 gtgggccggt tcctggacag acatcctttt gtgactctca ccgtgctgat gtttgttacc
      481 atgtcagcca tccctgttgg cttcttcctg ctcatcgtgg tgctcacctc gctgggtgct
      541 ctcatggggg ccatattatt ggaaggtctg gtcatctcag tttgtggtct ctcacttctc
      601 tgcgtcctct gtggcctggg cttcctgtca cttgccttgt cggggatagc catgatgtcc
      661 tatgtggtgg tctcctgcct catgagctac tggttttctc ccagcaggcc actgacacag
      721 caaaatgcca acgtcgactg tcagctggct atgaagttca cggagtctga gaggctcata
      781 ttttgagagg catgaccggg aatgacgggc caaatggaat caaagatggc ttcctaagca
      841 cccagaggag agcttgtccc ctcacctctc aggactgcag ggcagagaga gggctggatg
      901 ggagccctgc ttctctgctc actgtgtcca gactcctgca gtggccagcg tggcctcccc
      961 acccctcacg gctccccagt atgcagcctt gaccttagca gagtggtggg ctgctagaac
     1021 ttggaagctt ggcctttaga gccttgaggt tgagtcccag agcccatctg cggctaagat
     1081 agattgtatt tgaagtttag ttgcagtctg ggggcagggc agggatgaag aggacgggtt
     1141 ttctccagtc atttgaaata agaggttaaa aaaataatcc aaaggagcca gttcttactg
     1201 ttttgttttg tttttttatc agaagagcat cagcatttgg aaatggtggc cttttaaaac
     1261 ctgaaaatct gtcttaaccc tgcagccagg agtcctttag gaaacatctc aaggcctagt
     1321 ctattgcatg ccttgatctt tgacatattt gcacttgata tgttaatatt gtttaaagag
     1381 cttgtttctg ccaagccatt tcttaacaaa ccaataaaac cgaacacata ctgaagaaag
     1441 actacaaagc aaaccagagc ctgtgcgatg atttccactt catttactgt ggtattttat
     1501 taaatattta tttgcttctt
//
