LOCUS       NM_177846               3407 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus leucine carboxyl methyltransferase 2 (Lcmt2), mRNA.
ACCESSION   NM_177846 XM_001003239 XM_141327
VERSION     NM_177846.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Timmermans,S. and Libert,C.
  TITLE     Overview of inactivating mutations in the protein-coding genome of
            the mouse reference strain C57BL/6J
  JOURNAL   JCI Insight 3 (13), 121758 (2018)
   PUBMED   29997285
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   3  (bases 1 to 3407)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   4  (bases 1 to 3407)
  AUTHORS   De Baere,I., Derua,R., Janssens,V., Van Hoof,C., Waelkens,E.,
            Merlevede,W. and Goris,J.
  TITLE     Purification of porcine brain protein phosphatase 2A leucine
            carboxyl methyltransferase and cloning of the human homologue
  JOURNAL   Biochemistry 38 (50), 16539-16547 (1999)
   PUBMED   10600115
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845479.8.
            
            On or before Sep 7, 2008 this sequence version replaced
            XM_001003239.1, XM_141327.6, NM_177846.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK172970.1, AK084642.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3407              AL845479.8         49334-52740         c
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..3407
                     /gene="Lcmt2"
                     /gene_synonym="D330024M17; Tyw4"
                     /note="leucine carboxyl methyltransferase 2"
                     /db_xref="GeneID:329504"
                     /db_xref="MGI:MGI:1353659"
     exon            1..3407
                     /gene="Lcmt2"
                     /gene_synonym="D330024M17; Tyw4"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..2159
                     /gene="Lcmt2"
                     /gene_synonym="D330024M17; Tyw4"
                     /EC_number="2.1.1.290"
                     /EC_number="2.3.1.231"
                     /note="tRNA yW-synthesizing protein 4 homolog;
                     p21WAF1/CIP1 promoter-interacting protein; tRNA
                     wybutosine-synthesizing protein 4 homolog; tRNA(Phe)
                     (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-
                     methyltransferase; tRNA(Phe)
                     (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-
                     methoxycarbonyltransferase"
                     /codon_start=1
                     /product="tRNA wybutosine-synthesizing protein 4"
                     /protein_id="NP_808514.2"
                     /db_xref="CCDS:CCDS50682.1"
                     /db_xref="GeneID:329504"
                     /db_xref="MGI:MGI:1353659"
                     /translation="MGPRGRQRRAGTVQSTNDSSSLSKRSLAAHGYVRDPFAALLVPG
                     PVRRTPLIHRGYYVRARAVRHCVRAFLELTSALPSRTRAQILSLGSGSDSLYFRLKAA
                     GLLARAAVWEVDFPDVSRLKAERIEETPELRAQTGPFKIGDSASSLCFESADYRILGA
                     DLRELQRLGEALDGAGLDATSPTLLLAEAVLTYLEPSSATALIAWAAQRFPDALFVIY
                     EQMQPGDAFGQIMLQHFQRLHSPLHGLELFPVVKAQRQRFLQAGWTACSALDLNEFYR
                     RLLSAEERQRVETLEPFDEYEEWHLKCSHYFILAASRGDILSETPVFEPSEASFQIDP
                     ASPSGFLSARVVTSDHQHSSLKRYGHASALLSPGVIFSAGGFGEQEGRHCRVSRFHVL
                     SRSCDSEWEGCQISTLGTEGQWDGRLYHTMTRLSDTRVLVLGGRLSPVSPASGALQLD
                     LYKSKDNCSEGQNVTVTKAALEEGSVLSCWRHSTTEVYYQNQRYLFVYGGRSVAEPVL
                     SDCRFLHVETMAWVRIPVQGASPEGRHSHSACSWQGGALIAGGLGASEELLSSVLFLK
                     PVSSGFLWESIDIQPSITPRYSHTAHVFNGKLLLVGGVWIHSSSVPGVTVICLTTGLS
                     SEYQIDTASVPWPLMLHNHSSALLPEEQQLLLIGGGGNCFSFGTYFNPHTVALDLSSL
                     RSGQ"
     regulatory      2155..2160
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lcmt2"
                     /gene_synonym="D330024M17; Tyw4"
                     /note="hexamer: AATAAA"
     polyA_site      2176
                     /gene="Lcmt2"
                     /gene_synonym="D330024M17; Tyw4"
                     /note="major polyA site"
     regulatory      2761..2766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lcmt2"
                     /gene_synonym="D330024M17; Tyw4"
                     /note="hexamer: AATAAA"
     polyA_site      2786
                     /gene="Lcmt2"
                     /gene_synonym="D330024M17; Tyw4"
ORIGIN      
        1 ggcagagagc ggtcttctcg gaagtgacgc acccagaagc ggatgagcgg gagctcggta
       61 agtgttctgt gtgcagtatt gctgagcacc ttctcgtcat gggcccgcga ggccgccagc
      121 gccgggcggg aacggtccag agcaccaacg acagcagctc cctcagcaaa cgatcgcttg
      181 ccgcacacgg atacgtgcgc gacccctttg cggcgctgct ggtcccggga cccgtgcggc
      241 gcacgccgct tatccaccgc ggctattacg tgcgcgcgcg cgccgtgcgt cactgcgtgc
      301 gcgccttcct cgaactcact agcgcgctcc cgtcccggac cagagcccag atcctgtcct
      361 tgggctcagg ctccgactcg ctttattttc gtctgaaagc ggcgggcctc ctggcccgag
      421 ctgccgtctg ggaggtggac ttcccggacg tgtctcggct caaggcggag aggatcgagg
      481 agaccccgga gctgcgtgcg cagaccggtc ctttcaagat cggggactcg gcgtcatctc
      541 tgtgcttcga gagcgcggac taccgcatcc tgggcgcgga cctgcgggag ctccagcgat
      601 taggagaggc cctggatggc gccggcctgg acgccacctc tcccacgctg ctcctggccg
      661 aggcggtgct gacctacttg gagccctcca gtgccacagc cctcattgcc tgggccgccc
      721 agcgtttccc cgacgccctt ttcgtgatct atgagcagat gcagccgggg gatgcctttg
      781 ggcagatcat gctgcagcac tttcagcggt tgcattcacc cttgcatggc ctggagctgt
      841 ttcccgtcgt gaaggcccag cgccagcgct tccttcaagc tggctggact gcctgcagcg
      901 ccctggacct gaacgagttc tatcgccgcc ttctctcggc agaagagcgc cagcgggttg
      961 agacgcttga accctttgat gaatatgagg agtggcatct gaagtgttca cattatttca
     1021 tcctggcagc atctagggga gacattctgt cagaaactcc agtgtttgag ccctcagagg
     1081 cttcttttca gatagatcct gcctcgcctt cagggtttct ttctgccaga gtagtcacta
     1141 gtgaccacca gcactcaagc ctgaagcgat atggccacgc ctctgccctc ttgagccctg
     1201 gcgttatttt cagtgcagga ggctttggag aacaagaggg acgacactgc cgagtgagca
     1261 ggtttcacgt gctctccaga tcctgtgact cggaatggga aggctgccaa ataagtacct
     1321 tggggactga aggccagtgg gatggacgcc tttatcacac catgacaagg ctttctgata
     1381 ctcgggttct ggttcttgga ggaagactct ccccagtaag tccagcctct ggggctctcc
     1441 aacttgattt atacaaaagt aaggataatt gctctgaggg ccaaaatgta acagtaacga
     1501 aggctgcctt ggaagaaggt tcagtgttgt catgttggcg gcattcaaca acggaagtat
     1561 actatcagaa tcaaagatac ttatttgtgt atggtggccg aagtgtggct gaacctgtac
     1621 taagtgactg tcgttttcta catgtagaga caatggcttg ggtcagaatc ccagtacaag
     1681 gcgcatcacc cgaaggtcgg cattcccata gcgcctgcag ttggcaaggg ggagcactta
     1741 tcgctggagg tcttggggct tctgaggaac tgttgagctc tgtactcttt ctcaaaccag
     1801 tgtcctctgg attcctctgg gaatcaatag atatccagcc ctccattacc ccaaggtact
     1861 ctcacacggc tcatgtgttt aatggaaagc tgcttctggt tggaggggtc tggattcatt
     1921 cctcctcagt tcctggagtg actgttatct gtttgactac agggttgagc tctgaatatc
     1981 agattgacac agcgtctgtg ccatggccat taatgctgca caaccacagc agcgccctcc
     2041 ttcctgaaga gcagcagctc ctgcttattg gaggtggagg gaactgcttt tcctttggta
     2101 cttatttcaa cccccacaca gtggcattag acctttcttc cttacgttca gggcaataaa
     2161 gaatggactt gatatattta ggacctaagg gtgaatttgt tgtattgcca cactcccaac
     2221 acacacacct ttcttcctta ccacaccatt gagcagagac cataaaaatg atgattaaaa
     2281 tacagaccag cagtaagtat aaaaataaag catcaaaaga gtggcataca tcctaggggc
     2341 ctcagcatca tgtgaagagt tttctggggt tctatcagtt ttccagtctc agctctaaca
     2401 aggaaccagc acaactccta ggtctgagga cctattattt attgagtcac aacaaccttt
     2461 agcctaagta aaaaaaacaa aaacgaaaaa cattcccaaa cctgggtctg tgacctagat
     2521 tatattttgg aaaaagtttc acacaggtca tggtaaaaat ccacagctga gtatagttca
     2581 ggaatgtagt gttgacctgt tggctatggg caaacagtct aaaatgaaca ctgcagttta
     2641 aagactcgtg cttttagttt gaacactgaa ctttaacagt gagaaaaagt acaacttaga
     2701 tattatgctt ttgatcactt ttttgctaat gttttatatt tatctagctt tataagtgct
     2761 aataaaccag acatttaatg ccttaatttt gtaccttcta taatgaataa tgtttgttcc
     2821 tgtcagccgg acactgttct aaacccccaa gcagcttaga taggcagcat cagtccacaa
     2881 gtcagataag tactgtacta tgctaaacta tactatatac aagtactata agaaggggat
     2941 gtgtgttggg gggggctggg gagcaggaag tgagccacag tagttgtgct tttcctctgt
     3001 cagtctacag gttgatattg tgcattctac caaagccata ggcgctggca tgtaacctat
     3061 gtggtaagat agaacactaa tactacattg ctgcattgct taaggtttta ggagttgttc
     3121 cttttccttg ccctcttaag agttgtgctg gctccttgtt agagctgagc tgcattacgc
     3181 aatatacttt taattacttt tcttgcttta ctgtgaagaa ataacatgac cgggctggtg
     3241 agatggctca gtgggtaaga gcacccgact gctcttctga aggtccagag ttcaaatccc
     3301 agcaaccaca tggtggctca caaccatccc taacaagatc tggcgccctc ttctggagtg
     3361 tctgaagaca gctacagtgt acttacatat aataaataaa taaatct
//
