LOCUS       NM_001355050            1361 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus leucine carboxyl methyltransferase 1 (Lcmt1),
            transcript variant 2, mRNA.
ACCESSION   NM_001355050 XM_006507917
VERSION     NM_001355050.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Mo,J., Wang,X., Liang,N., Zhang,N., Li,Y., Zheng,Z., Ao,Q., Wu,Y.,
            Tang,T., Liao,S., Lei,Y., Ding,H., Du,B., Feng,M., Chen,C., Shi,Q.,
            Wei,L., Huang,Y., Lu,C., Tang,S. and Li,X.
  TITLE     Hepatic leucine carboxyl methyltransferase 1 (LCMT1) contributes to
            high fat diet-induced glucose intolerance through regulation of
            glycogen metabolism
  JOURNAL   J Nutr Biochem 117, 109321 (2023)
   PUBMED   36963730
  REMARK    GeneRIF: Hepatic leucine carboxyl methyltransferase 1 (LCMT1)
            contributes to high fat diet-induced glucose intolerance through
            regulation of glycogen metabolism.
REFERENCE   3  (bases 1 to 1361)
  AUTHORS   Gnanaprakash,M., Staniszewski,A., Zhang,H., Pitstick,R.,
            Kavanaugh,M.P., Arancio,O. and Nicholls,R.E.
  TITLE     Leucine Carboxyl Methyltransferase 1 Overexpression Protects
            Against Cognitive and Electrophysiological Impairments in Tg2576
            APP Transgenic Mice
  JOURNAL   J Alzheimers Dis 79 (4), 1813-1829 (2021)
   PUBMED   33459709
  REMARK    GeneRIF: Leucine Carboxyl Methyltransferase 1 Overexpression
            Protects Against Cognitive and Electrophysiological Impairments in
            Tg2576 APP Transgenic Mice.
REFERENCE   4  (bases 1 to 1361)
  AUTHORS   Staniszewski,A., Zhang,H., Asam,K., Pitstick,R., Kavanaugh,M.P.,
            Arancio,O. and Nicholls,R.E.
  TITLE     Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A
            Methyltransferase, LCMT-1, Alters Sensitivity to
            Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments
            in Mice
  JOURNAL   J Neurosci 40 (23), 4596-4608 (2020)
   PUBMED   32341098
  REMARK    GeneRIF: Reduced Expression of the PP2A Methylesterase, PME-1, or
            the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to
            Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments
            in Mice.
REFERENCE   5  (bases 1 to 1361)
  AUTHORS   Lee,J.A., Wang,Z., Sambo,D., Bunting,K.D. and Pallas,D.C.
  TITLE     Global loss of leucine carboxyl methyltransferase-1 causes severe
            defects in fetal liver hematopoiesis
  JOURNAL   J Biol Chem 293 (25), 9636-9650 (2018)
   PUBMED   29735529
  REMARK    GeneRIF: Loss of Lcmt1 expression is associated with severe defects
            in fetal liver hematopoiesis.
REFERENCE   6  (bases 1 to 1361)
  AUTHORS   Sontag,J.M., Nunbhakdi-Craig,V., Mitterhuber,M., Ogris,E. and
            Sontag,E.
  TITLE     Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1
            plays a critical role in differentiation of neuroblastoma cells
  JOURNAL   J Neurochem 115 (6), 1455-1465 (2010)
   PUBMED   21044074
  REMARK    GeneRIF: Enhanced expression of LCMT1 in cultured neuroblastoma
            cells, which increases endogenous methylated catalytic C subunit
            and Balpha levels, induces changes in F-actin organization.
REFERENCE   7  (bases 1 to 1361)
  AUTHORS   Lee,J.A. and Pallas,D.C.
  TITLE     Leucine carboxyl methyltransferase-1 is necessary for normal
            progression through mitosis in mammalian cells
  JOURNAL   J Biol Chem 282 (42), 30974-30984 (2007)
   PUBMED   17724024
  REMARK    GeneRIF: LCMT-1 is important for normal progression through mitosis
            and cell survival and is essential for embryonic development
REFERENCE   8  (bases 1 to 1361)
  AUTHORS   Nord,A.S., Chang,P.J., Conklin,B.R., Cox,A.V., Harper,C.A.,
            Hicks,G.G., Huang,C.C., Johns,S.J., Kawamoto,M., Liu,S., Meng,E.C.,
            Morris,J.H., Rossant,J., Ruiz,P., Skarnes,W.C., Soriano,P.,
            Stanford,W.L., Stryke,D., von Melchner,H., Wurst,W., Yamamura,K.,
            Young,S.G., Babbitt,P.C. and Ferrin,T.E.
  TITLE     The International Gene Trap Consortium Website: a portal to all
            publicly available gene trap cell lines in mouse
  JOURNAL   Nucleic Acids Res 34 (Database issue), D642-D648 (2006)
   PUBMED   16381950
REFERENCE   9  (bases 1 to 1361)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 1361)
  AUTHORS   De Baere,I., Derua,R., Janssens,V., Van Hoof,C., Waelkens,E.,
            Merlevede,W. and Goris,J.
  TITLE     Purification of porcine brain protein phosphatase 2A leucine
            carboxyl methyltransferase and cloning of the human homologue
  JOURNAL   Biochemistry 38 (50), 16539-16547 (1999)
   PUBMED   10600115
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121956.3.
            
            On Sep 6, 2017 this sequence version replaced XM_006507917.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BG870400.1, SRR1660815.101655.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-619               AC121956.3         43287-43905         c
            620-722             AC121956.3         40896-40998         c
            723-843             AC121956.3         33384-33504         c
            844-945             AC121956.3         30083-30184         c
            946-1037            AC121956.3         28924-29015         c
            1038-1135           AC121956.3         23531-23628         c
            1136-1361           AC121956.3         21364-21589         c
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 67.42 cM"
     gene            1..1361
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /note="leucine carboxyl methyltransferase 1"
                     /db_xref="GeneID:30949"
                     /db_xref="MGI:MGI:1353593"
     exon            1..619
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            620..722
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             628..1158
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /EC_number="2.1.1.233"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="leucine carboxyl methyltransferase 1 isoform 2"
                     /protein_id="NP_001341979.1"
                     /db_xref="GeneID:30949"
                     /db_xref="MGI:MGI:1353593"
                     /translation="MLDSKRYAIIGADLRDLSELEEKLKKCNMNTQLPTLLITECVLV
                     YMTPEQSANLLKWAASSFETAMFINYEQVNMDDRFGQIMIENLRRRSCDLAGVETCKS
                     LESQKERLLLNGWETASAVNMMELYSGLPRAEVNRIESLEFLDEMELLEQLMRHYCLC
                     WATRGGQELGLKEITY"
     exon            723..843
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            844..945
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1037
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1135
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1361
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1326..1331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /note="hexamer: AATAAA"
     polyA_site      1352
                     /gene="Lcmt1"
                     /gene_synonym="Lcmt; LCMT-1"
                     /note="major polyA site"
ORIGIN      
        1 gtccattatg ctccctgtga caataaagag gctctctctg aggacagaaa atggaccttg
       61 tatatctaaa caactggaac agcttggact tagggaaaca gtatatggtg ctagtgcatg
      121 ctgttctagt cctcagagtc tgtcacatca aaggctcaag gtcatgttgt ccagggcttc
      181 ctgcctgttg tgtggatgct gggcctgaga gcctcagatc actgtgtgca ttcacagcta
      241 ctgtccctgt gcacgtttgc tgtttgatat tggtcctagt aagataagtc tctcccaaac
      301 cacttggctg ccccgtccca cctttgtgtt atattatttc cattatatta gagaactgca
      361 tgcctcagga ttctgttgtg ttcttcaagt ttttgttgga aagggaagaa actcattaaa
      421 gacttgcttg ctatcaatgt attgttttta agaaggcaca caggcacacg gggattgttt
      481 gtttctgtta taagccagac aagtgctttt gtattcttta tattattttc ttgatacagt
      541 gttgtggtgc cctggggctg actctgagtt ccctgtttgt cctttattgc tttcctgaca
      601 tctaagggac tcccagaaga tagtcacatg ctggattcaa aaagatatgc catcattgga
      661 gcagatctcc gagacctttc tgagctggaa gagaaactaa agaaatgtaa catgaataca
      721 caattgccaa cactcctgat aactgaatgt gtgctggttt acatgactcc agagcagtca
      781 gccaacctcc tgaaatgggc agccagcagt tttgagacag ccatgttcat aaactatgag
      841 caggtcaaca tggacgaccg gtttgggcag atcatgattg aaaacctccg gaggcgaagt
      901 tgtgacctgg caggagtgga aacctgcaaa tcactggagt cccagaagga acggctgctg
      961 ttgaatgggt gggagacagc gtctgcagtg aacatgatgg agctgtacag tgggctacct
     1021 cgggcagagg taaacagaat agaatccctc gagttcctgg atgagatgga gctcctggag
     1081 cagctcatgc gccattactg cctgtgctgg gccacccgcg gcggccagga gcttggtttg
     1141 aaggagataa cctattgatc tgctgaaggc tcgtgctggg gtggaaggtg aaggctctag
     1201 ctaagggagt gccagggcct cctgagagat gtgcagcctc actttgttgg tcctgctgca
     1261 catccggaac ccgtggttac aaaggtggtc acaccagatc ctgttcctgt cagtatcatt
     1321 gtttaaataa atctcacttg ccatttgtgt cagactctgc t
//
