LOCUS       NM_001177967            4455 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus lysocardiolipin acyltransferase 1 (Lclat1), transcript
            variant 2, mRNA.
ACCESSION   NM_001177967
VERSION     NM_001177967.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Zhang,J. and Shi,Y.
  TITLE     An upstream open reading frame (5'-uORF) links oxidative stress to
            translational control of ALCAT1 through phosphorylation of
            eIF2alpha
  JOURNAL   Free Radic Biol Med 214, 129-136 (2024)
   PUBMED   38360278
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4455)
  AUTHORS   Ren,W., Xu,Z., Pan,S., Ma,Y., Li,H., Wu,F., Bo,W., Cai,M. and
            Tian,Z.
  TITLE     Irisin and ALCAT1 mediated aerobic exercise-alleviated oxidative
            stress and apoptosis in skeletal muscle of mice with myocardial
            infarction
  JOURNAL   Free Radic Biol Med 193 (Pt 2), 526-537 (2022)
   PUBMED   36336228
  REMARK    GeneRIF: Irisin and ALCAT1 mediated aerobic exercise-alleviated
            oxidative stress and apoptosis in skeletal muscle of mice with
            myocardial infarction.
REFERENCE   4  (bases 1 to 4455)
  AUTHORS   Liu,X., Zhang,J., Li,J., Song,C. and Shi,Y.
  TITLE     Pharmacological inhibition of ALCAT1 mitigates amyotrophic lateral
            sclerosis by attenuating SOD1 protein aggregation
  JOURNAL   Mol Metab 63, 101536 (2022)
   PUBMED   35772643
  REMARK    GeneRIF: Pharmacological inhibition of ALCAT1 mitigates amyotrophic
            lateral sclerosis by attenuating SOD1 protein aggregation.
REFERENCE   5  (bases 1 to 4455)
  AUTHORS   Song,C., Zhang,J., Qi,S., Liu,Z., Zhang,X., Zheng,Y.,
            Andersen,J.P., Zhang,W., Strong,R., Martinez,P.A., Musi,N., Nie,J.
            and Shi,Y.
  TITLE     Cardiolipin remodeling by ALCAT1 links mitochondrial dysfunction to
            Parkinson's diseases
  JOURNAL   Aging Cell 18 (3), e12941 (2019)
   PUBMED   30838774
  REMARK    GeneRIF: ALCAT1 expression is upregulated by MPTP and by
            alpha-synucleinopathy, a key hallmark of Parkinson's disease (PD),
            whereas ALCAT1 deficiency prevented alpha-synuclein oligomerization
            and S-129 phosphorylation, implicating a key role of ALCAT1 in the
            etiology of mouse models of PD.
REFERENCE   6  (bases 1 to 4455)
  AUTHORS   Calvo,S.E., Pagliarini,D.J. and Mootha,V.K.
  TITLE     Upstream open reading frames cause widespread reduction of protein
            expression and are polymorphic among humans
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7507-7512 (2009)
   PUBMED   19372376
  REMARK    GeneRIF: Includes the study of an upstream ORF in this gene, and
            shows that it functions to reduce protein levels by ~40%.
REFERENCE   7  (bases 1 to 4455)
  AUTHORS   Wang,C., Faloon,P.W., Tan,Z., Lv,Y., Zhang,P., Ge,Y., Deng,H. and
            Xiong,J.W.
  TITLE     Mouse lysocardiolipin acyltransferase controls the development of
            hematopoietic and endothelial lineages during in vitro embryonic
            stem-cell differentiation
  JOURNAL   Blood 110 (10), 3601-3609 (2007)
   PUBMED   17675553
  REMARK    GeneRIF: Mouse Lycat plays an important role in the early
            specification of hematopoietic and endothelial cells.
REFERENCE   8  (bases 1 to 4455)
  AUTHORS   Agarwal,A.K., Barnes,R.I. and Garg,A.
  TITLE     Functional characterization of human 1-acylglycerol-3-phosphate
            acyltransferase isoform 8: cloning, tissue distribution, gene
            structure, and enzymatic activity
  JOURNAL   Arch Biochem Biophys 449 (1-2), 64-76 (2006)
   PUBMED   16620771
REFERENCE   9  (bases 1 to 4455)
  AUTHORS   Cao,J., Liu,Y., Lockwood,J., Burn,P. and Shi,Y.
  TITLE     A novel cardiolipin-remodeling pathway revealed by a gene encoding
            an endoplasmic reticulum-associated acyl-CoA:lysocardiolipin
            acyltransferase (ALCAT1) in mouse
  JOURNAL   J Biol Chem 279 (30), 31727-31734 (2004)
   PUBMED   15152008
  REMARK    GeneRIF: identification and characterization of a gene that encodes
            an acyl-CoA:lysocardiolipin acyltransferase 1
REFERENCE   10 (bases 1 to 4455)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB868193.1, BC147499.1, AC112949.9 and AI847050.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC147499.1, ERR3363660.976260.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            regulatory uORF        :: PMID: 19372376
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-61                BB868193.1         2-62
            62-1862             BC147499.1         1-1801
            1863-4230           AC112949.9         49274-51641         c
            4231-4455           AI847050.1         14-238              c
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 44.79 cM"
     gene            1..4455
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /note="lysocardiolipin acyltransferase 1"
                     /db_xref="GeneID:225010"
                     /db_xref="MGI:MGI:2684937"
     exon            1..172
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      70..78
                     /regulatory_class="uORF"
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19372376]"
                     /note="this uORF is predicted to encode a 2 aa peptide
                     (PMID:19372376)"
     exon            173..341
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /inference="alignment:Splign:2.1.0"
     exon            342..540
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /inference="alignment:Splign:2.1.0"
     exon            541..687
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /inference="alignment:Splign:2.1.0"
     exon            688..804
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /inference="alignment:Splign:2.1.0"
     exon            805..4455
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4425..4430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /note="hexamer: AATAAA"
     polyA_site      4455
                     /gene="Lclat1"
                     /gene_synonym="1-AGPAT 8; Agpat8; Alcat1; Gm91; Lycat"
                     /note="major polyA site"
ORIGIN      
        1 gccaatcagg gtggagcccg gagggacgca ttactaaggc gacggggctc gacgcctccc
       61 cgcttcggga tgaattagcg gcgggttctt ctcccaggtt gtgacccccg tggagtcgct
      121 gtctcgcccc agtgtccccg cgacgtcgcg cgtttccccc ggtgtgtccc agaatcatgg
      181 tgtcatggaa ggggatttac tttatactct tcctgtttgc tggaagcttt tttggaagta
      241 tttttatgct cggccccatt ttacctttga tgtttataaa cctgtcgtgg tatcgctgga
      301 ttagcagccg tcttgtggct acatggctca cacttcctgt ggcattgctg gagaccatgt
      361 ttggtgtgag agtggttata acaggtgacg cctttgtgcc tggagagcgg agcgtcatca
      421 tcatgaacca ccggacacgt gtggactgga tgttcctgtg gaactgtcta atgaggtaca
      481 gctacctcag ggtggagaag atttgcctca aatccagtct caaaagtgtt cctggattcg
      541 gctgggccat gcaagttgcg gcctttatct ttattcatag gaagtggaag gatgataaga
      601 gccattttga agacatgatt gattattttt gtgccatcca tgaaccacta cagcttctca
      661 tttttccaga aggaactgac ctcacagaaa ataataaggc taggagtaat gattttgctg
      721 agaagaacgg acttcagaaa tatgagtatg ttttacaccc aagaaccact ggctttacct
      781 ttgtggtgga ccgcctaaga gaagggaaga acctcgatgc tgttcatgac atcacggtcg
      841 catatcctta caacatccct caaactgaga agcaccttct ccttggagac tttcccaagg
      901 agatccactt ccacgtccag cggtatccag ctgactctct tcccacatcc aaggaggacc
      961 ttcagctctg gtgccacaga aggtgggaag aaaaggagga gaggcttcgg tccttctacc
     1021 aaggagagaa aaacttccac tttactgggc agagtacagt tccaccttgc aagtctgagc
     1081 tcagagtcct tgtggtcaag ctactgtcca tagtgtactg ggccttgttc tgctctgcaa
     1141 tgtgcctgct catatatctg tacagccctg ttcggtggta ttttataatc agcattgtgt
     1201 tcttcgtgct gcaggagaga atatttggtg gactggaaat cattgaactt gcatgttacc
     1261 gttttttaca caagcaccca catttaaatt caaagaaaaa tgagtaagat ggcggcgttc
     1321 atgtgaaaac ctagggttgt cacgggaatg tcagtctttg taaactgaga cgagttttgc
     1381 atgactatgt caaatgtttc ttactatcat cattttttgt taaaggtatt ttgcacttaa
     1441 ttttatgggg gaaatattgc tatggtttct ttaaaatctc tgaatgtaat tccaatgctg
     1501 agtatgtagc aggggtcaat cgctgtgaat tactagggtc agagtattac aaacaatcac
     1561 taagctgtca agacaaaatg caagcttttc tgaaaggacc aacctgtttc tgacagactt
     1621 ttggctcaga ggtagccttg gcaccatgaa caccagcccc agtgtatcta agcaggtagt
     1681 agtctgcctc ccccgcagga cacagtccat tctttaattc ttttacacca atcaaacgca
     1741 aaggtggact tgccttgtcc taagctgagc cccattggca gggagccctc catcactgat
     1801 agtctcttgt gctgagtgag gcaggaaaag accccctttc ctcgcctgga aatcacatct
     1861 gcactcagct cagggagcac cgtcaggctc tccatttggc ttttaaaaga cagaaactgg
     1921 tgcagcttgc ttttcactca cactcagtcc tgccttcctg atggtcacca gggagcgtca
     1981 tctgtagcgt ggactggttc atgtaaagat gcattagaaa aacatctgta gttcagacag
     2041 aaactggaag gcaggtgtct gtcctatgca tgcagggtgt ctgccttctg ccattagagc
     2101 tacaacaaac atgcctgcag acaggatggt gtagctaaag cccccaaacc ttgataactt
     2161 tactcttaaa acaaaaactg ctaactcaga atgagctctt tcagtgatag caaggacaca
     2221 ctctctcttt ttagtcatct ctcaacagtg acatttaaac acacactgac ctactgtctg
     2281 ccaaatattc ctatcatttg gaagcagtga caacatagca aaccaatagc gagcacgcag
     2341 tgtcctgggt aagagaatcc ttgtggccac tgtttccact ccactgagct gcatgctccc
     2401 atctgtgtca tgtgattggg gcagggacag aggcacatag aagcttcccc accccacccc
     2461 accccagtat ccaacagtct cagcagctct taactgccct gtgtgctcag acagaaatct
     2521 gcctttccta agatggcagt gcgcatctgt acctggtaag gacttcctca tcttgccaag
     2581 cacactgaga aagccccgtt ggggacagga caccatcagc atagcgtggt taagtctggc
     2641 tttttgatgt ttgtgcattt gttagtggga gagtgctaac tggtgcctct ggagaaacaa
     2701 gccagcccat gtttaactgc tccatcaaaa ctcactttaa tcctgctggg aacttactaa
     2761 ttagtagctg tgcacacctg ccctcaacac agctgtttga ctccctagtt tttaaggtta
     2821 cacacacaca accctccccc cacccttttt tctttccctt aatgagaaag gcttccaatt
     2881 ttaagtttct ctgatggatc acatttaaat gtggaaatag ctagcaattt gttgcccagg
     2941 aaaaagagaa atgctgtcat ctttgcccac agtagttggg ctcggttaac agtgctgtcc
     3001 atctcattgg gcacccacag gctctctgtc tcttgttctt cccttcgccc tcttctgatc
     3061 atagaaaaca ttaaggtaga gaatacctta attttacttt acctacccca aattcctaat
     3121 acagcactaa aactagagaa tgtggacact ctacttgctt taagaggaat ttctccggag
     3181 ggggaaaata aaaataaacg tgtttttaaa ttataattat ttgttcttac ctacacgttt
     3241 aactgtatta aaactgttta tttaatttaa aaaaatgcat atacacctaa tgcttttgag
     3301 cttggaactg ctttaagtca atacttgagc atcaggccta agcagatgtg gccttgatgg
     3361 ttgtcagcct aaggaatgcc cctgccacac cttcagccag ggctcagcag ctcagccagg
     3421 gctcagcagc tcagccgtgc tccagcagac tctttccctg ctccctgtgc aggtgagctg
     3481 aagtgctcgc agcactagtt ggctagttga tcccattgga agagatttta catatagtag
     3541 ttgtgggtta tttataatgt gaaccatttc accctaaaat atgaaccgat atagaggctg
     3601 ggtgatgagt tcccacattg tgtgtctttg atgaaacaaa cccagggaat cctaaacttt
     3661 agtcaagcaa catggcggga gagactgaaa atgaagtaga aacatatcta tctgcgagag
     3721 cttctcactt aagactctcc ctaaaatcct gcctctgcac gcccacagaa caaacccgag
     3781 aaggaagcag cgccttccac tcgtgttcta atggtgacca cgccacacgc agaccctatg
     3841 ttggtataag tctgaattct gcagattggt tccatttcac cggctctcaa gcccaacaaa
     3901 gaagctctgg aacagtcaca ataaggacta gcagagggat cctgtcggtg ttaaactgtt
     3961 tacatttctt tatatacaat aatgtgcttt cagtgcctta gttgtgggtc cctttctttt
     4021 tcctgttcta agaattgtat accgtgttct tagataaagt ctctcctcgt cactcactag
     4081 gaggaaagtg gtgcctcaat accagtgggt cagcggttga ctgaaacaaa acaaaacgct
     4141 atgttgtaac atggagaagg gagctcggtg ctgagctatg gctgcctagt tcttacacag
     4201 tggcaaacaa ttgcttggcc acgaagtgga cttcacacca cgtgtggccc taaaaggtgt
     4261 cttgtaccta cctaaaattt tttatttcag agtcttggtt ttggtaactt ctttttaaaa
     4321 gatcattaca cggagaacac ccaagtttta aaaataactc acagaatggc cttagcttca
     4381 gtgtccactg ggatatttgt gaattacctg taaaatgcat ccagaataaa atggagtgaa
     4441 ttacatggtt caaaa
//
