LOCUS       NM_001348167            4219 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus leucyl-tRNA synthetase, mitochondrial (Lars2),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001348167
VERSION     NM_001348167.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Patel,S.D., Anand,D., Motohashi,H., Katsuoka,F., Yamamoto,M. and
            Lachke,S.A.
  TITLE     Deficiency of the bZIP transcription factors Mafg and Mafk causes
            misexpression of genes in distinct pathways and results in lens
            embryonic developmental defects
  JOURNAL   Front Cell Dev Biol 10, 981893 (2022)
   PUBMED   36092713
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Wang,Z., Lu,Z., Lin,S., Xia,J., Zhong,Z., Xie,Z., Xing,Y., Qie,J.,
            Jiao,M., Li,Y., Wen,H., Zhao,P., Zhang,D., Zhou,P., Qian,J.,
            Luo,F., Wang,L., Yu,H., Liu,J., Gu,J., Liu,R. and Chu,Y.
  TITLE     Leucine-tRNA-synthase-2-expressing B cells contribute to colorectal
            cancer immunoevasion
  JOURNAL   Immunity 55 (6), 1067-1081 (2022)
   PUBMED   35659337
  REMARK    GeneRIF: Leucine-tRNA-synthase-2-expressing B cells contribute to
            colorectal cancer immunoevasion.
            Erratum:[Immunity. 2022 Sep 13;55(9):1748. doi:
            10.1016/j.immuni.2022.07.017.. PMID: 36103860]
REFERENCE   3  (bases 1 to 4219)
  AUTHORS   Jung,H., Park,H., Choi,Y., Kang,H., Lee,E., Kweon,H., Roh,J.D.,
            Ellegood,J., Choi,W., Kang,J., Rhim,I., Choi,S.Y., Bae,M.,
            Kim,S.G., Lee,J., Chung,C., Yoo,T., Park,H., Kim,Y., Ha,S.,
            Um,S.M., Mo,S., Kwon,Y., Mah,W., Bae,Y.C., Kim,H., Lerch,J.P.,
            Paik,S.B. and Kim,E.
  TITLE     Sexually dimorphic behavior, neuronal activity, and gene expression
            in Chd8-mutant mice
  JOURNAL   Nat Neurosci 21 (9), 1218-1228 (2018)
   PUBMED   30104731
REFERENCE   4  (bases 1 to 4219)
  AUTHORS   Chang,Y., Stockinger,M.P., Tashiro,H. and Lin,C.L.
  TITLE     A novel noncoding RNA rescues mutant SOD1-mediated cell death
  JOURNAL   FASEB J 22 (3), 691-702 (2008)
   PUBMED   17957031
REFERENCE   5  (bases 1 to 4219)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   6  (bases 1 to 4219)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 4219)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 4219)
  AUTHORS   Kiss,H., Darai,E., Kiss,C., Kost-Alimova,M., Klein,G.,
            Dumanski,J.P. and Imreh,S.
  TITLE     Comparative human/murine sequence analysis of the common eliminated
            region 1 from human 3p21.3
  JOURNAL   Mamm Genome 13 (11), 646-655 (2002)
   PUBMED   12461651
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164123.4 and AC125254.9.
            
            On Nov 9, 2022 this sequence version replaced NM_001348167.1.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.210799.1,
                                           SRR17784644.243408.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-504               AC164123.4         8643-9146           c
            505-753             AC164123.4         3979-4227           c
            754-882             AC125254.9         23314-23442
            883-974             AC125254.9         38396-38487
            975-1035            AC125254.9         40564-40624
            1036-1125           AC125254.9         55227-55316
            1126-1269           AC125254.9         57486-57629
            1270-1377           AC125254.9         63792-63899
            1378-1537           AC125254.9         64248-64407
            1538-1642           AC125254.9         73060-73164
            1643-1758           AC125254.9         77499-77614
            1759-2042           AC125254.9         81730-82013
            2043-2141           AC125254.9         83748-83846
            2142-2279           AC125254.9         87077-87214
            2280-2380           AC125254.9         98358-98458
            2381-2563           AC125254.9         98824-99006
            2564-2733           AC125254.9         100323-100492
            2734-2811           AC125254.9         100573-100650
            2812-2923           AC125254.9         101506-101617
            2924-3051           AC125254.9         105105-105232
            3052-4219           AC125254.9         107112-108279
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 73.92 cM"
     gene            1..4219
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /note="leucyl-tRNA synthetase, mitochondrial"
                     /db_xref="GeneID:102436"
                     /db_xref="MGI:MGI:2142973"
     exon            1..504
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            505..753
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     CDS             523..3231
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /EC_number="6.1.1.4"
                     /note="isoform 1 is encoded by transcript variant 2;
                     probable leucine--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="probable leucine--tRNA ligase, mitochondrial
                     isoform 1"
                     /protein_id="NP_001335096.1"
                     /db_xref="CCDS:CCDS23659.1"
                     /db_xref="GeneID:102436"
                     /db_xref="MGI:MGI:2142973"
                     /translation="MASTCQRLSFYVSPLKRQLVSRPPVILWERLIPGCSRSIYSATG
                     KWTKEYTLQTRKDVEKWWHQQIKEQASRVSEEDKLKPKFYLLSMFPYPSGKLHMGHVR
                     VYTLSDTIARFQKMRGMQVINPMGWDAFGLPAENAAIERNLHPESWTQSNIKHMRKQL
                     DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQ
                     VNEYGCSWRSGAKVEKKYLRQWFIKTTAYAKAMQDALADLPEWYGIKGMQAHWIGDCV
                     GCHLDFTLKVDGEDTGEKLTAYTATPEAIYGISHVAISPSHGLLHGCSSVKKALQKAL
                     VPGRDCLTPVMAVSMLTLQEVPIVIMANPDLEGSLDSKIGIPSTSSEDTRLAQALGLP
                     YSEVIEASPDGTERLSGSAEFTGMTRQDAFVALTRKARGMRVGGHVTSNKLKDWLISR
                     QRYWGTPIPIVHCPACGPVPVPLQDLPVILPSIASLTGRGGSPLATALEWVNCSCPRC
                     KGSAKRETDTMDTFVDSAWYYFRYTDPHNTQSPFGSALADFWMPVDLYIGGKEHAVMH
                     LFYARFLSHFCHDQKMVKHREPFHKLLAQGLIKGQTFRLPSGQCLKKEDIDFTGPAPV
                     CAKTKEKLEVTWEKMSKSKHNGVDPEEIVAQYGIDTIRLYILFAAPPEKDILWDVKTD
                     ALPGVLRWQQRLWSLTTRFIEARTSGTVPQPQLLNSKEKTKAQNLWEYKNAVIAQVTT
                     HFTEDFALNSVVSQLMGLSSALSQASQRVVLHSPEFEDALCALLVMAAPLAPHVTSEL
                     WAGLTLVPSKLCDHYAWDSGVMLQAWPTVDSQFLQKPDMVQMAVLINNKACGKIPVPQ
                     HVAQDQDKVHELVLQSELGMKLLQGRSIKKAFLSPRTALINFLVQE"
     exon            754..882
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            883..974
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1035
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1125
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1269
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1377
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1537
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1642
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1758
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..2042
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2141
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2279
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2280..2380
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2381..2563
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..2733
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2811
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2923
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            2924..3051
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     exon            3052..4219
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4199..4204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /note="hexamer: AATAAA"
     polyA_site      4219
                     /gene="Lars2"
                     /gene_synonym="Kiaa0028; LEURS"
                     /note="major polyA site"
ORIGIN      
        1 gttgttttgc caacatggcg gcgcccagag tgactggctg ggtcttgctc gtgtaatgct
       61 tggggaacga ggcagaaggt agcagctggc cagacagagc ctgagcgcag aaagccccgc
      121 ctggggctca ggaagacgag agacaagccc cgcttagccc ccggagctga aggtgcggac
      181 aggctcggga gtcccgggac cttggagttt gacaaaatgg gaaaagctcc gagattctag
      241 tctataggct ctttcagcca tggaccctgt tgacagctcc ggactcttgc tcggagaacg
      301 cacatcttca catggctttg tattcgaatt ctcctctact actcacctcc ccccgggtgc
      361 ctggatcttc tcttcggggt tgggggtttg gacccagaaa aggaccatca gtactacatc
      421 tttgggggac ccacagtatt aattaggacc cgcaggtctt cacaagtcgc ctggatctta
      481 acaggctcac agcttacaga gaagggtctt ctctcctgaa gaatggcatc cacttgtcag
      541 aggctgagtt tctatgtctc tcctctgaaa aggcagctag ttagcaggcc acctgtcatc
      601 ctgtgggaaa gactaattcc agggtgttcc agaagtatct acagtgccac cgggaagtgg
      661 acaaaggagt acacactgca gacaaggaag gatgtggaga agtggtggca ccaacagata
      721 aaggagcaag cgtccagagt ctcagaggaa gataaattaa agcccaaatt ctacttgctc
      781 tccatgttcc catacccttc tggcaagctg cacatgggcc atgtccgtgt ctacaccctc
      841 agcgacacca ttgcacggtt ccagaaaatg agaggcatgc aggttatcaa ccccatgggg
      901 tgggatgcat ttggattgcc tgctgaaaat gctgccatag agaggaattt gcaccctgaa
      961 agctggaccc aaagcaatat taaacacatg aggaagcagc tcgaccgcct cggcctgtgc
     1021 ttcagctggg acagggaaat cactacatgt ttgccagatt actacaaatg gactcaatac
     1081 ctgtttatca aactctatga agcaggactg gcctatcaaa aggaggcctt ggttaactgg
     1141 gatccagtgg atcaaacagt gctagccaat gagcaggtga atgaatatgg ctgttcatgg
     1201 cgttctggag ccaaggtaga gaagaaatac ctccgacagt ggttcattaa gacaactgct
     1261 tatgcaaagg ccatgcagga tgcgttggca gacctcccag agtggtacgg aatcaaaggc
     1321 atgcaggccc actggattgg ggactgtgta ggctgtcacc tggacttcac attaaaggtt
     1381 gatggagaag acacaggaga gaaattgaca gcatacacag ccacccctga ggccatctat
     1441 ggcatctccc acgtggccat ctctcctagc cacgggcttc tacatggctg cagctctgtg
     1501 aagaaagcct tgcagaaggc actagtccct ggcagagatt gcctcacacc agtgatggct
     1561 gtgagcatgc tcaccctgca ggaggtcccc atcgttatca tggctaaccc tgacttggaa
     1621 ggctctctag attcaaaaat aggaatcccc agcaccagct cagaggacac cagactggcc
     1681 caagctctgg gcctacccta ctctgaggtc attgaagctt cacccgatgg cacagagaga
     1741 ctgagtggat ctgctgagtt tacaggtatg accaggcagg atgcctttgt agccctgact
     1801 cggaaagccc gtgggatgag agtgggtgga cacgtgacaa gtaacaagct gaaggactgg
     1861 ctgatctcac ggcagcgcta ctggggcaca ccgatcccca ttgtccactg cccagcctgt
     1921 ggccctgtgc ctgtgccact gcaagacttg cctgtgattt tgcccagcat tgcatcttta
     1981 acgggcagag gaggctcccc gctggccacg gctttggagt gggtgaactg ctcctgccca
     2041 aggtgcaagg gatcagcaaa gagagagacg gacaccatgg acacctttgt ggattctgcg
     2101 tggtactact tcagatacac tgaccctcat aatactcaaa gtccttttgg ctcggcactg
     2161 gcagacttct ggatgcccgt ggatctgtac atcggaggaa aggaacatgc tgtcatgcac
     2221 ttgttctatg cacgattcct cagtcatttt tgccacgacc aaaagatggt gaagcacagg
     2281 gaacctttcc ataaactact ggcccaaggc ctcattaagg gacagacatt ccgccttcca
     2341 tctggacagt gtctgaagaa ggaggatata gatttcacag gtcctgctcc tgtttgtgca
     2401 aaaacaaaag agaagttaga ggtgacgtgg gagaagatga gcaagtccaa acacaatggg
     2461 gtggaccctg aggagattgt ggcccaatat ggaattgaca caatccggct ctacatcctc
     2521 tttgcagccc ctcctgagaa ggacatcttg tgggatgtaa agactgacgc actccctgga
     2581 gtgctaagat ggcaacagcg gttgtggtcc ctgacaacaa gattcatcga ggcaaggact
     2641 tccgggacag tccctcagcc tcagctgctg aacagcaagg agaaaaccaa ggcccaaaac
     2701 ctctgggagt acaaaaatgc agtcatagct caggtgacca ctcatttcac agaggacttt
     2761 gccctgaatt ctgtagtttc tcagctgatg ggactcagca gtgccctctc gcaagcctct
     2821 cagagagtgg ttctccacag ccctgagttt gaagatgctc tgtgtgccct gctggtgatg
     2881 gctgctcccc tggcccctca tgtaacctca gaactttggg caggcctgac gctggtacca
     2941 agcaagctct gtgaccatta tgcctgggat tctggtgtga tgcttcaggc ctggcccact
     3001 gtggactcac agttccttca gaaacctgac atggtgcaga tggctgttct gatcaacaac
     3061 aaggcctgtg gcaagatccc tgtgccccag catgttgccc aggaccagga caaagtccat
     3121 gaacttgttc tccagagcga gctaggcatg aagctgctgc aggggagaag catcaagaag
     3181 gccttcctct cccccaggac tgccctcatc aacttcttgg tgcaggagtg accagggctt
     3241 ctaggaccct ccttctctgc ccagatcaag ggaaggaatg ttaacgtggg aaaaaaggca
     3301 aacatcaggg acattggcct ttgtgccagg accagcagga gtcagcagga ggcctaaggc
     3361 gagctcaggg aggacagaaa cctcccgtgg agcagaaggg caaaagctcg cttgatcttg
     3421 attttcagta ggaatacaga ccgtgaaagc ggggcctcac gatccttctg accttttggg
     3481 ttttaagcag gatgtgtcag aaaagttacc acagggataa ctggcttgtg gcggccaagc
     3541 gttcatagcg acgtcgcttt ttgatccttc gatgtcggct cttcctatca ttgtgaagca
     3601 gaattcacca agcgttggat tgttcaccca ctaataggga acgtgagctg ggattagacc
     3661 gtcgtgagac aggttagttt taccctactg atgatgtgtt gttgccatgg taatcctgct
     3721 cagtacgaga ggaaccgcag ctagcttgtg tctccagtgt cttttaccat cacaggccct
     3781 ggctctgtgc tacctcccta gaatacctgt ggttcatcac cttcagctca cactccattc
     3841 agacaatgct cacttaggcc tacaagcaag ggagaggctt aagcacaagg tctttaagct
     3901 tgatactgtg cctggtctca atgcctagaa attccaactt aactgtcgct atcataaagg
     3961 attttaaatt tttttacaag tgagaaccta gcatgtcaag gtcctaggtt ccatctccaa
     4021 cacgaaaaaa gcaaggcaac tctaccaaag aaactaatta catacttgta ttcccaatat
     4081 gtgagaagct gaggaaggag gatcatgagt ttaaagccat ccttggttac atgagactct
     4141 tgtctcaaag aactaccagg gatcctaatg gacaacaaag gttgaaaacc attgaattaa
     4201 taaatgttgt gaaccataa
//
