LOCUS       NM_172670               2453 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus LARGE xylosyl- and glucuronyltransferase 2 (Large2),
            transcript variant 1, mRNA.
ACCESSION   NM_172670
VERSION     NM_172670.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Inamori,K.I., Beedle,A.M., de Bernabe,D.B., Wright,M.E. and
            Campbell,K.P.
  TITLE     LARGE2-dependent glycosylation confers laminin-binding ability on
            proteoglycans
  JOURNAL   Glycobiology 26 (12), 1284-1296 (2016)
   PUBMED   27496765
  REMARK    GeneRIF: The LARGE2 may play a differential role in stabilizing the
            basement membrane and modifying its functions by augmenting the
            interactions between laminin globular domain-containing ECM
            proteins and proteoglycans (PGs).
REFERENCE   3  (bases 1 to 2453)
  AUTHORS   Inamori,K., Willer,T., Hara,Y., Venzke,D., Anderson,M.E.,
            Clarke,N.F., Guicheney,P., Bonnemann,C.G., Moore,S.A. and
            Campbell,K.P.
  TITLE     Endogenous glucuronyltransferase activity of LARGE or LARGE2
            required for functional modification of alpha-dystroglycan in cells
            and tissues
  JOURNAL   J Biol Chem 289 (41), 28138-28148 (2014)
   PUBMED   25138275
  REMARK    GeneRIF: Endogenous glucuronyltransferase activity of LARGE or
            LARGE2 required for functional modification of alpha-dystroglycan
            in cells and tissues.
REFERENCE   4  (bases 1 to 2453)
  AUTHORS   Lee,J.T., Pamir,N., Liu,N.C., Kirk,E.A., Averill,M.M., Becker,L.,
            Larson,I., Hagman,D.K., Foster-Schubert,K.E., van Yserloo,B.,
            Bornfeldt,K.E., LeBoeuf,R.C., Kratz,M. and Heinecke,J.W.
  TITLE     Macrophage metalloelastase (MMP12) regulates adipose tissue
            expansion, insulin sensitivity, and expression of inducible nitric
            oxide synthase
  JOURNAL   Endocrinology 155 (9), 3409-3420 (2014)
   PUBMED   24914938
REFERENCE   5  (bases 1 to 2453)
  AUTHORS   Yagi,H., Nakagawa,N., Saito,T., Kiyonari,H., Abe,T., Toda,T.,
            Wu,S.W., Khoo,K.H., Oka,S. and Kato,K.
  TITLE     AGO61-dependent GlcNAc modification primes the formation of
            functional glycans on alpha-dystroglycan
  JOURNAL   Sci Rep 3, 3288 (2013)
   PUBMED   24256719
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2453)
  AUTHORS   Ashikov,A., Buettner,F.F., Tiemann,B., Gerardy-Schahn,R. and
            Bakker,H.
  TITLE     LARGE2 generates the same xylose- and glucuronic acid-containing
            glycan structures as LARGE
  JOURNAL   Glycobiology 23 (3), 303-309 (2013)
   PUBMED   23135544
REFERENCE   7  (bases 1 to 2453)
  AUTHORS   Inamori,K., Hara,Y., Willer,T., Anderson,M.E., Zhu,Z.,
            Yoshida-Moriguchi,T. and Campbell,K.P.
  TITLE     Xylosyl- and glucuronyltransferase functions of LARGE in
            alpha-dystroglycan modification are conserved in LARGE2
  JOURNAL   Glycobiology 23 (3), 295-302 (2013)
   PUBMED   23125099
REFERENCE   8  (bases 1 to 2453)
  AUTHORS   Grewal,P.K., McLaughlan,J.M., Moore,C.J., Browning,C.A. and
            Hewitt,J.E.
  TITLE     Characterization of the LARGE family of putative
            glycosyltransferases associated with dystroglycanopathies
  JOURNAL   Glycobiology 15 (10), 912-923 (2005)
   PUBMED   15958417
REFERENCE   9  (bases 1 to 2453)
  AUTHORS   Grewal,P.K. and Hewitt,J.E.
  TITLE     Mutation of Large, which encodes a putative glycosyltransferase, in
            an animal model of muscular dystrophy
  JOURNAL   Biochim Biophys Acta 1573 (3), 216-224 (2002)
   PUBMED   12417403
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731709.19, AY742914.1 and AI594129.1.
            
            On Mar 19, 2014 this sequence version replaced NM_172670.2.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            CCDS Note: The coding region has been updated to represent an
            alternate exon combination and to extend the N-terminus to one that
            is more supported by available conservation data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY742914.1, SRR13422598.1223364.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AL731709.19        152812-152835       c
            25-1457             AY742914.1         1-1433
            1458-1458           AL731709.19        148287-148287       c
            1459-2420           AY742914.1         1435-2396
            2421-2453           AI594129.1         332-364
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 50.97 cM"
     gene            1..2453
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /note="LARGE xylosyl- and glucuronyltransferase 2"
                     /db_xref="GeneID:228366"
                     /db_xref="MGI:MGI:2443769"
     exon            1..77
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            78..232
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..2205
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /note="isoform 1 is encoded by transcript variant 1;
                     glycoslytransferase-like 1B; glycosyltransferase-like
                     protein LARGE2; glycosyltransferase-like 1B; xylosyl- and
                     glucuronyltransferase LARGE2"
                     /codon_start=1
                     /product="xylosyl- and glucuronyltransferase LARGE2
                     isoform 1"
                     /protein_id="NP_766258.2"
                     /db_xref="CCDS:CCDS16443.2"
                     /db_xref="GeneID:228366"
                     /db_xref="MGI:MGI:2443769"
                     /translation="MLPRGRPRAMGAAVLLLLLLLVVGFFLFGRDPDYGLGTTATLDE
                     DPYRSRNLSASSPQLLLPPKCEMLHVAIVCAGYNSSREIITLTKSLLFYRKNPLHLHL
                     ITDAVARNILETLFRTWMVPAVVVSFYDAEELKPLVSWIPNKHYSGLYGLMKLVLPSI
                     LPPSLARVIVLDTDVTFSSDIVELWALFDHFSDKQVVGLVENQSDWYLGNLWKNHRPW
                     PALGRGFNTGVILLWLDRLQQTGWEQMWKVTAKRELLTLMATSLADQDIFNAVIKEHP
                     HLVHPLPCVWNVQLSDHTRAERCYLEAADLKVIHWNSPKKLRVKNKHAEFFRNLHLTF
                     LGYDGKLLRRELFGCPNQFPPGAEQLQQALTQLDEEEPCFEFRQQQLTVHRVHITFLP
                     HQPPPPQPHDVTLVAQLSMDRLQMLEALCRHWPGPMSLALYLTDEEAQQFLHFVETSP
                     VLSMRKDVAYHVVYRDGPLYPVNQLRNVALAQALTPYVFLSDIDFLPAYSLYDYLRAS
                     IEQLELDSRRKTALVVPAFETLHYRFSFPNSKAELLTLLDAGSLHTFRYHEWPQGHSS
                     TDYSRWREAQAPYSVQWSADYEPYVVVPRDCPRYDPRFVGFGWNKVAHIIELDAQEYE
                     FLVLPEAFSIHLPHAPSLDISRFRSSPTYRNCLQALKEEFHQDLSRRYGSAALKYLTA
                     LQQARSRA"
     exon            233..330
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            331..413
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            414..537
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            538..709
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            710..814
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            815..927
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1053
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1209
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1370
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1649
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1799
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1995
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2445
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2417..2422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /note="hexamer: AATAAA"
     polyA_site      2445
                     /gene="Large2"
                     /gene_synonym="5730485C17Rik; Gyltl1b; Largel; mKIAA4105"
                     /note="major polyA site"
ORIGIN      
        1 agttcgcgat tctgaccggg acctgactgc agggcggtcg caggaggaac agggccttcc
       61 cggagccact ggagcagggt cggtgatgga gcccgcagcc cggggtcgcg ccccgccctg
      121 agtcccacag tcatgctgcc ccgaggtcgc ccccgggcaa tgggggccgc cgtgctgctg
      181 ctgctgctgt tgctagtggt tggcttcttc ctgttcggcc gggacccgga ttatggactg
      241 ggcacaactg ctaccctcga tgaagacccg tacaggagtc gcaacctctc cgcctccagc
      301 ccgcagcttc tactgccacc caagtgcgag atgttgcatg tggctatcgt gtgtgcggga
      361 tacaactcca gccgagagat tattacccta acgaagtccc tgctattcta caggaaaaat
      421 ccgctgcacc tccacctgat aactgatgcc gtagccagaa acatcctgga gacactcttc
      481 cgaacatgga tggtgccagc ggtggtggtc agcttctatg atgcggaaga actcaagccc
      541 ctggtctcct ggatccccaa caaacactac tctggcctct atgggctaat gaagctagta
      601 cttcccagca tcctgcctcc cagcctggcc cgagtcatcg tcctggatac cgacgtcact
      661 ttctcctctg acattgtgga gctctgggca ctctttgatc atttttctga caagcaggtg
      721 gtcggtctcg tggagaacca gagcgactgg tacctgggca acctctggaa gaaccatagg
      781 ccctggcctg ccttgggcag gggatttaac acaggtgtga tcctgctgtg gctggacagg
      841 ctccagcaaa ctggctggga gcagatgtgg aaggtgacag ccaaacgaga gctgctcact
      901 ctgatggcta cttccttggc tgaccaggac atcttcaatg cggtcatcaa ggagcacccc
      961 catctggtgc accccctgcc ctgtgtctgg aacgtgcagc tgtcagacca cactcgggct
     1021 gagcgctgct acctggaagc agctgacctc aaagtgatcc actggaattc accaaagaag
     1081 cttcgagtga agaacaagca cgcagaattc ttccgtaatc tgcacttgac ctttctgggg
     1141 tatgatggga agctactgcg aagagagctc tttggatgcc ccaaccagtt ccctcctggg
     1201 gccgagcagt tgcaacaggc cctaacacag ctggatgagg aagagccctg ctttgagttc
     1261 cgccaacagc agctcactgt gcaccgggtg cacatcacct tcctgcccca ccagccgcca
     1321 cctccccagc ctcacgatgt caccttggtg gcccaactct ctatggaccg gctgcagatg
     1381 ctggaagccc tgtgcaggca ctggccaggc cccatgagcc tggccttgta cctgacagat
     1441 gaagaggctc aacaatttct tcattttgtg gaaacgtcgc cagtgctctc tatgaggaag
     1501 gatgtggcct accatgtagt gtaccgggac ggtccactct atccagtcaa ccagctccgc
     1561 aacgtggcct tggcccaggc tctcacaccc tacgtcttcc tcagtgatat tgacttctta
     1621 cctgcctact ccctctacga ctacctcagg gcttctatcg agcagctgga gctggacagt
     1681 cggcgcaaga ctgctttggt ggtgcctgca tttgagaccc tacactaccg gttcagcttc
     1741 ccaaactcta aggcagagct gttgacgtta ctggatgccg gctcccttca cacctttagg
     1801 taccacgagt ggccacaggg tcactcatcc acagactatt cccgctggcg ggaagcccag
     1861 gcaccataca gtgtgcagtg gtcagctgac tatgaaccct acgtggtggt accccgtgac
     1921 tgcccccgtt atgatcctcg ctttgtggga tttggctgga acaaggtggc ccacatcata
     1981 gagttggatg ctcaggaata tgaattcctg gtacttcctg aggccttctc tatccacttg
     2041 ccccacgctc caagtcttga catctcccgc ttccgctcca gccccaccta ccgcaactgt
     2101 ctccaggccc tcaaggaaga gttccaccag gacttgtcaa ggcgctatgg gtctgcagcc
     2161 ctgaaatacc tcactgccct gcagcaggcc cgaagtcggg cctgagctta ccccgctgcc
     2221 ctagctatgg cttacacatt ccctgtagag gctgaccagg gtgtccaccc ttactgctat
     2281 ttaagttatt taaaattttt gtcagaaggg gcagagtgga gcatttgtgg ggttcctggt
     2341 tccttctgct gatccacact gggatccagc cctcctttgc ctcagctggt ttggggctag
     2401 tctaattctc ttatataata aaaattttca atttttttta tcttaaaaaa aaa
//
