LOCUS       NM_008483               5605 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus laminin, beta 2 (Lamb2), transcript variant 1, mRNA.
ACCESSION   NM_008483
VERSION     NM_008483.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Wang,M., Liu,H., Zhang,M., Niu,X., Sun,M., Wang,F., Ni,Y., Hong,T.,
            Zhang,W. and Dang,S.
  TITLE     The secreted protease ADAMTS18 links early isoform transformation
            and maturation of glomerular basement membrane macromolecules to
            the integrity of the glomerular filtration barrier
  JOURNAL   Biochem Biophys Res Commun 750, 151386 (2025)
   PUBMED   39879696
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5605)
  AUTHORS   Ramadan,R., Wouters,V.M., van Neerven,S.M., de Groot,N.E.,
            Garcia,T.M., Muncan,V., Franklin,O.D., Battle,M., Carlson,K.S.,
            Leach,J., Sansom,O.J., Boulard,O., Chamaillard,M., Vermeulen,L.,
            Medema,J.P. and Huels,D.J.
  TITLE     The extracellular matrix controls stem cell specification and crypt
            morphology in the developing and adult mouse gut
  JOURNAL   Biol Open 11 (12) (2022)
   PUBMED   36350252
REFERENCE   4  (bases 1 to 5605)
  AUTHORS   Serjanov,D., Bachay,G., Hunter,D.D. and Brunken,W.J.
  TITLE     Laminin beta2 Chain Regulates Cell Cycle Dynamics in the Developing
            Retina
  JOURNAL   Front Cell Dev Biol 9, 802593 (2022)
   PUBMED   35096830
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5605)
  AUTHORS   Yang,Z.S., Pan,H.Y., Shi,W.W., Chen,S.T., Wang,Y., Li,M.Y.,
            Zhang,H.Y., Yang,C., Liu,A.X. and Yang,Z.M.
  TITLE     Regulation and Function of Laminin A5 during Mouse and Human
            Decidualization
  JOURNAL   Int J Mol Sci 23 (1), 199 (2021)
   PUBMED   35008625
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5605)
  AUTHORS   Aeschlimann,D. and Paulsson,M.
  TITLE     Cross-linking of laminin-nidogen complexes by tissue
            transglutaminase. A novel mechanism for basement membrane
            stabilization
  JOURNAL   J Biol Chem 266 (23), 15308-15317 (1991)
   PUBMED   1678389
REFERENCE   7  (bases 1 to 5605)
  AUTHORS   Chung,A.E. and Durkin,M.E.
  TITLE     Entactin: structure and function
  JOURNAL   Am J Respir Cell Mol Biol 3 (4), 275-282 (1990)
   PUBMED   2119632
  REMARK    Review article
REFERENCE   8  (bases 1 to 5605)
  AUTHORS   Sasaki,M., Kleinman,H.K., Huber,H., Deutzmann,R. and Yamada,Y.
  TITLE     Laminin, a multidomain protein. The A chain has a unique globular
            domain and homology with the basement membrane proteoglycan and the
            laminin B chains
  JOURNAL   J Biol Chem 263 (32), 16536-16544 (1988)
   PUBMED   3182802
REFERENCE   9  (bases 1 to 5605)
  AUTHORS   Paulsson,M., Aumailley,M., Deutzmann,R., Timpl,R., Beck,K. and
            Engel,J.
  TITLE     Laminin-nidogen complex. Extraction with chelating agents and
            structural characterization
  JOURNAL   Eur J Biochem 166 (1), 11-19 (1987)
   PUBMED   3109910
REFERENCE   10 (bases 1 to 5605)
  AUTHORS   Elliott,R.W., Barlow,D. and Hogan,B.L.
  TITLE     Linkage of genes for laminin B1 and B2 subunits on chromosome 1 in
            mouse
  JOURNAL   In Vitro Cell Dev Biol 21 (8), 477-484 (1985)
   PUBMED   2993224
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT010508.10.
            
            On Aug 23, 2022 this sequence version replaced NM_008483.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026051.1, SRR9219380.48931.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                CT010508.10        129644-129737
            95-180              CT010508.10        129821-129906
            181-353             CT010508.10        130041-130213
            354-489             CT010508.10        130301-130436
            490-563             CT010508.10        130519-130592
            564-752             CT010508.10        130997-131185
            753-816             CT010508.10        131267-131330
            817-1019            CT010508.10        131571-131773
            1020-1140           CT010508.10        131864-131984
            1141-1329           CT010508.10        132271-132459
            1330-1509           CT010508.10        132644-132823
            1510-1622           CT010508.10        132934-133046
            1623-1702           CT010508.10        133293-133372
            1703-1835           CT010508.10        133451-133583
            1836-1994           CT010508.10        133774-133932
            1995-2122           CT010508.10        134009-134136
            2123-2249           CT010508.10        134950-135076
            2250-2442           CT010508.10        135261-135453
            2443-2586           CT010508.10        135530-135673
            2587-2818           CT010508.10        135878-136109
            2819-2982           CT010508.10        136185-136348
            2983-3207           CT010508.10        136427-136651
            3208-3425           CT010508.10        136739-136956
            3426-3522           CT010508.10        137056-137152
            3523-3895           CT010508.10        137239-137611
            3896-4080           CT010508.10        137710-137894
            4081-4322           CT010508.10        137968-138209
            4323-4671           CT010508.10        138289-138637
            4672-4879           CT010508.10        138930-139137
            4880-5021           CT010508.10        139216-139357
            5022-5198           CT010508.10        139540-139716
            5199-5358           CT010508.10        139809-139968
            5359-5605           CT010508.10        140047-140293
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.4 cM"
     gene            1..5605
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /note="laminin, beta 2"
                     /db_xref="GeneID:16779"
                     /db_xref="MGI:MGI:99916"
     exon            1..94
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            95..180
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..5495
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /note="laminin subunit beta-2; S-LAM beta; S-laminin
                     subunit beta; laminin-3 subunit beta; laminin-4 subunit
                     beta; laminin-7 subunit beta; laminin-9 subunit beta;
                     laminin-11 subunit beta; laminin-14 subunit beta;
                     laminin-15 subunit beta; Laminin S"
                     /codon_start=1
                     /product="laminin subunit beta-2 precursor"
                     /protein_id="NP_032509.2"
                     /db_xref="CCDS:CCDS23528.1"
                     /db_xref="GeneID:16779"
                     /db_xref="MGI:MGI:99916"
                     /translation="MEWASGEPGRGRQGQPLPWELRLGLLLSVLAATLAQAPSLDVPG
                     CSRGSCYPATGDLLVGRADRLTASSTCGLHSPQPYCIVSHLQDEKKCFLCDSRRPFSA
                     RDNPNSHRIQNVVTSFAPQRRTAWWQSENGVPMVTIQLDLEAEFHFTHLIMTFKTFRP
                     AAMLVERSADFGRTWHVYRYFSYDCGADFPGIPLAPPRRWDDVVCESRYSEIEPSTEG
                     EVIYRVLDPAIPIPDPYSSRIQNLLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYA
                     LYELVIRGNCFCYGHASQCAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYQDL
                     PWHPAEDGHTHACRKCECNGHTHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCEFC
                     RPFFYRDPTKDMRDPAVCRPCDCDPMGSQDGGRCDSHDDPVLGLVSGQCRCKEHVVGT
                     RCQQCRDGFFGLSASDPRGCQRCQCNSRGTVPGSSPCDSSSGTCFCKRLVTGHGCDRC
                     LPGHWGLSHDLLGCRPCDCDVGGALDPQCDEATGQCRCRQHMIGRRCEQVQPGYFRPF
                     LDHLTWEAEAAQGQGLEVVERLVTNRETPSWTGPGFVRLREGQEVEFLVTSLPRAMDY
                     DLLLRWEPQVPEQWAELELMVQRPGPVSAHSPCGHVLPKDDRIQGMLHPNTRFLVFPR
                     PVCLEPGISYKLKLKLIGTGGRAQPETSYSGLLIDSLVLQPHVLVLEMFSGGDAAALE
                     RRTTFERYRCHEEGLMPSKAPLSETCAPLLISVSALIYNGALPCQCDPQGSLSSECSP
                     HGGQCRCKPGVVGRRCDVCATGYYGFGPAGCQACQCSPDGALSALCEGTSGQCPCRPG
                     AFGLRCDHCQRGQWGFPNCRPCVCNGRADECDTHTGACLGCRDYTGGEHCERCIAGFH
                     GDPRLPYGGQCRPCPCPEGPGSQRHFATSCHRDGYSQQIVCQCREGYTGLRCEACAPG
                     HFGDPSKPGGRCQLCECSGNIDPMDPDACDPHTGQCLRCLHNTEGPHCGYCKPGFHGQ
                     AARQSCHRCTCNLLGTDPRRCPSTDLCHCDPSTGQCPCLPHVQGLNCDHCAPNFWNFT
                     SGRGCQPCACHPSRARGPTCNEFTGQCHCHAGFGGRTCSECQELYWGDPGLQCRACDC
                     DPRGIDKPQCHRSTGHCSCRPGVSGVRCDQCARGFSGVFPACHPCHACFGDWDRVVQD
                     LAARTRRLEQWAQELQQTGVLGAFESSFLNMQGKLGMVQAIMSARNASAASTAKLVEA
                     TEGLRHEIGKTTERLTQLEAELTAVQDENFNANHALSGLERDGLALNLTLRQLDQHLE
                     ILKHSNFLGAYDSIRHAHSQSTEAERRANASTFAVPSPVSNSADTRRRTEVLMGAQKE
                     NFNRQHLANQQALGRLSAHAHTLSLTGINELVCGAPGDAPCATSPCGGAGCRDEDGQP
                     RCGGLGCSGAAATADLALGRARHTQAELQRALVEGGGILSRVSETRRQAEEAQQRAQA
                     ALDKANASRGQVEQANQELRELIQNVKDFLSQEGADPDSIEMVATRVLDISIPASPEQ
                     IQRLASEIAERVRSLADVDTILAHTMGDVRRAEQLLQDAHRARSRAEGERQKAETVQA
                     ALEEAQRAQGAAQGAIWGAVVDTQNTEQTLQRVQERMAGAEKSLNSAGERARQLDALL
                     EALKLKRAGNSLAASTAEETAGSAQSRAREAEKQLREQVGDQYQTVRALAERKAEGVL
                     AAQARAEQLRDEARDLLQAAQDKLQRLQELEGTYEENERALEGKAAQLDGLEARMRSV
                     LQAINLQVQIYNTCQ"
     exon            181..353
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            354..489
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            490..563
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            564..752
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            753..816
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            817..1019
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1140
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1329
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1509
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1622
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1702
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1835
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1994
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2122
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2249
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2442
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2586
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2818
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..2982
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            2983..3207
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            3208..3425
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            3426..3522
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            3523..3895
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            3896..4080
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            4081..4322
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            4323..4671
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            4672..4879
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            4880..5021
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            5022..5198
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            5199..5358
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     exon            5359..5605
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5585..5590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /note="hexamer: AATAAA"
     polyA_site      5605
                     /gene="Lamb2"
                     /gene_synonym="Lamb-2; Lams; npht"
                     /note="major polyA site"
ORIGIN      
        1 agagtctctc agattgtgga ggagtgactg ctaggtccga ccctggacag attgagggcc
       61 tggagggaca ccagcccagt acccacacgg tcgggatgga gtgggcctca ggagaaccag
      121 ggaggggcag gcagggacag cctttgccat gggaacttcg cttgggccta cttctaagtg
      181 tgctggctgc cacattggcc caggccccgt ccttggatgt acctggctgt tctcgaggaa
      241 gctgctatcc agccaccggt gacctgttgg tgggccgtgc ggacagactg acggcctcat
      301 ccacgtgtgg cttgcatagc cctcaaccct actgtattgt cagtcacctg caggacgaaa
      361 agaagtgttt cctgtgtgac tcccgacgtc ccttctctgc tcgagacaac ccaaatagtc
      421 atcggatcca gaatgtagtc accagctttg cgccacaacg ccggacagcc tggtggcaat
      481 cggagaacgg ggttccaatg gtcaccatcc aactggacct ggaagctgag tttcatttca
      541 cccacctcat tatgacgttc aagacgttcc ggcctgctgc tatgctggtg gagcgttctg
      601 cagactttgg ccgcacctgg cacgtgtacc gatatttttc ctatgactgc ggggctgact
      661 tcccgggaat cccactggcc ccgccacgtc gctgggatga tgtagtgtgt gagtcccgct
      721 actcagaaat cgagccgtct acggaaggcg aggtcatcta tcgtgtgctg gaccctgcta
      781 ttcctatccc agacccctac agctcacgga ttcagaacct gttgaagatc accaacctac
      841 gagtgaactt aacccggctt cacacactgg gagacaactt gcttgaccca cggagggaga
      901 tccgggaaaa atactattat gctctctatg aacttgtcat ccgtggcaac tgcttctgct
      961 atggccacgc ctcacagtgt gcgcctgcac caggggcgcc ggcccatgct gagggcatgg
     1021 tacacggagc ctgtatctgc aagcacaata ctcgtggact caactgtgag cagtgtcagg
     1081 atttctatca ggaccttccc tggcaccctg cagaggacgg ccatactcac gcctgtcgga
     1141 agtgtgagtg caacgggcat actcatagct gccactttga catggctgtc tacctggcat
     1201 ctggaaatgt aagtggaggc gtatgcgatg ggtgtcagca caacacagct gggcgccatt
     1261 gtgagttctg ccggcccttc ttctaccgtg accccaccaa ggacatgcgg gacccagctg
     1321 tgtgccgtcc ttgtgactgt gaccctatgg gttctcaaga tggtggtcgc tgtgattctc
     1381 atgatgaccc tgtgctagga ctggtctcag gccagtgtcg ctgcaaagaa cacgtggttg
     1441 gcactcgctg ccagcaatgc cgtgatggct tctttggact tagtgccagt gaccctcgag
     1501 ggtgccagcg ttgccagtgt aattcacggg gcacagtgcc tgggagctcc ccttgtgact
     1561 ccagtagtgg aacctgtttc tgcaagcgtc tggtgaccgg acatggctgt gaccgctgtc
     1621 tgcctggcca ctggggcctg agccatgacc tgctgggctg ccgtccctgt gactgtgatg
     1681 tgggcggtgc cttggatcct cagtgtgatg aggccaccgg tcagtgccgc tgccgccaac
     1741 acatgattgg gcggcgctgc gaacaagtgc agcctggcta cttccggcct tttctggacc
     1801 atttaacctg ggaggctgag gctgcccaag ggcaggggct tgaggtggta gagcggctgg
     1861 tgaccaaccg agagactccg tcctggactg gcccaggctt tgtgcggctg cgagaaggtc
     1921 aggaagtgga gttcctggtg acctctttgc ctagggccat ggactatgac ctgctactgc
     1981 gctgggagcc ccaggtccct gagcaatggg cagagctgga actgatggtg cagcgtccgg
     2041 ggcctgtgtc tgctcacagt ccgtgcgggc atgtgctgcc taaggatgac cgcattcagg
     2101 ggatgcttca cccaaacacc aggtttttgg tgtttcccag acctgtctgc cttgagcctg
     2161 gcatctccta caagctgaag ctgaaactga tcggaacagg gggacgagcc cagcctgaaa
     2221 cctcctactc tggattactc attgactcgc tggtcctgca gccccacgtc ttggtgctag
     2281 agatgtttag tgggggcgat gctgctgctc tggagcgccg taccaccttt gaacgctacc
     2341 gctgccatga ggaaggtctg atgcccagca aggcccctct atctgagacc tgtgcccccc
     2401 tcctcatcag cgtgtccgcc ttgatctaca atggcgcctt gccatgtcag tgtgaccctc
     2461 aaggctcact gagttctgaa tgcagtcctc acggtggcca gtgccggtgc aaacctggag
     2521 tggttggacg ccgttgtgat gtctgtgcta ctggctacta tggctttggc cctgcaggct
     2581 gtcaagcctg ccagtgtagt cctgatggag cactcagtgc cctctgtgaa gggactagtg
     2641 gacagtgccc ctgccgacct ggtgcctttg gtcttcgctg tgaccactgt caacgtggcc
     2701 agtggggatt ccctaattgc cggccgtgtg tctgcaatgg gcgtgcggat gagtgtgata
     2761 cccacacagg cgcttgcctg ggctgccgtg attacacggg gggcgagcac tgtgaaaggt
     2821 gcattgctgg ttttcatggg gacccacggc tgccatatgg gggccagtgc cggccttgtc
     2881 cctgccctga aggccctggg agccagcgac actttgctac ttcttgccac cgggatggat
     2941 attcccagca aattgtgtgc cagtgtcgag aaggctacac agggcttcgg tgtgaagctt
     3001 gtgcccccgg gcactttggg gacccatcaa agccaggtgg caggtgccaa ctgtgtgagt
     3061 gcagtggaaa cattgatccc atggaccctg atgcctgtga tccccacacg gggcaatgct
     3121 tgcgttgttt acacaacaca gaggggcccc actgtggcta ttgcaagcct ggcttccatg
     3181 ggcaagctgc ccgacagagc tgtcaccgct gtacctgcaa ccttctgggc acagatccca
     3241 ggcggtgccc atctaccgac ctgtgccatt gtgacccaag cactgggcag tgcccatgcc
     3301 ttccccatgt ccaaggcctc aactgtgacc attgtgcccc caacttttgg aacttcacca
     3361 gtggccgtgg ctgccagcct tgtgcttgtc acccaagccg ggccagaggc cctacctgca
     3421 atgagttcac agggcagtgt cactgtcatg ctggctttgg tgggaggact tgttctgagt
     3481 gccaagagct ctactgggga gaccctggtc tgcagtgccg tgcctgtgac tgtgatccta
     3541 gaggaataga caaacctcag tgtcatcgtt ccacaggcca ctgtagctgc cgcccaggcg
     3601 tgtctggtgt gcgctgtgac cagtgtgctc gtggcttctc aggggttttt cctgcttgtc
     3661 acccctgcca cgcttgcttt ggagactggg atcgtgtggt acaggacctg gctgctcgga
     3721 cgcggcgcct ggagcagtgg gctcaggagt tgcagcaaac aggagtgctg ggtgcctttg
     3781 agagcagctt tttgaacatg caggggaagc taggcatggt gcaggccatt atgagtgccc
     3841 gcaatgcctc agccgcctct acggcgaagc ttgtagaggc cacagaggga ctacgtcatg
     3901 aaatcgggaa gaccaccgag cgcctgactc agttagaagc agagctaaca gctgtgcagg
     3961 atgagaactt caatgccaac catgcactca gtggtctgga gagagacggg cttgcgctta
     4021 atctcaccct gaggcagctg gatcagcatc tggagatcct caaacattca aatttcttag
     4081 gtgcctatga cagcatccga catgcccaca gccagtccac agaggcagag cgccgtgcca
     4141 acgcctccac ctttgcagta cccagccctg tgagcaactc agcagatacc cggcgtcgga
     4201 cggaagtgct aatgggtgcc caaaaagaaa acttcaaccg ccaacatttg gccaaccagc
     4261 aggcactggg acggctctct gcacatgccc acaccctgag cctgacgggc ataaatgagt
     4321 tggtgtgtgg ggcaccaggg gacgcaccct gtgccaccag cccttgtggg ggtgccggat
     4381 gtcgggatga agatgggcag ccccgttgtg gtggcctcgg ttgcagtggg gcagcagcca
     4441 cggcagatct agcgctgggc cgggctcggc acacgcaggc agagctgcag cgggcactgg
     4501 tagaaggtgg cggcatcctc agccgggtgt ctgagactcg tcggcaggca gaagaggcac
     4561 agcagcgagc acaggcagcc ctggacaagg ctaatgcttc caggggccag gtggaacagg
     4621 ccaatcagga gcttcgagaa cttatccaga atgtgaaaga cttcctcagc caggagggag
     4681 ccgatcctga cagtattgaa atggtagcga ctcgggtgct agacatctcc atcccggcct
     4741 cacccgagca gatccagcgc ctagccagtg agattgcaga acgcgtccga agcctggccg
     4801 acgtggacac aatcctggcc cataccatgg gcgacgtgcg tcgggctgaa cagctactgc
     4861 aagatgcgca ccgggcacgg agccgggccg agggtgagag acagaaggca gagacagtcc
     4921 aagcggcact ggaggaggct cagagggcac aaggagctgc tcagggtgcc atctggggag
     4981 cagtggttga cacacaaaac acagagcaga ccctgcagcg ggtccaggag aggatggcag
     5041 gtgcagagaa gtctctgaac tctgccggtg agcgggctcg gcaattagac gccctcctgg
     5101 aggccctgaa actgaaacgg gcaggaaata gcctggcagc atctacagcg gaagaaacag
     5161 caggcagtgc ccagagccgt gccagggagg ctgagaaaca actacgggaa caagtaggtg
     5221 accaatacca aacagtgagg gcgttggcag agcggaaggc tgaaggtgtt ctggctgcac
     5281 aagccagggc agaacaactg cgggatgagg ctcgggacct gttgcaggcc gctcaggata
     5341 agctgcagcg gctacaggag ctggagggca catatgagga gaacgagcgt gcactggagg
     5401 gcaaagcggc ccagctggat gggctggaag ccaggatgcg cagtgtgctc caggccatca
     5461 acttgcaggt ccagatctac aacacctgcc agtgaccact ccctagggcc tagccttgtc
     5521 gccaagcact gttctgcaca cgatcgtccg cacattaaag agctcctggc tagcaagagc
     5581 tttcaataaa cctgtgtgaa cctcc
//
