LOCUS       NM_008482               5784 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus laminin B1 (Lamb1), mRNA.
ACCESSION   NM_008482
VERSION     NM_008482.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Wang,M., Liu,H., Zhang,M., Niu,X., Sun,M., Wang,F., Ni,Y., Hong,T.,
            Zhang,W. and Dang,S.
  TITLE     The secreted protease ADAMTS18 links early isoform transformation
            and maturation of glomerular basement membrane macromolecules to
            the integrity of the glomerular filtration barrier
  JOURNAL   Biochem Biophys Res Commun 750, 151386 (2025)
   PUBMED   39879696
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Miao,L., Lu,Y., Nusrat,A., Zhao,L., Castillo,M., Xiao,Y., Guo,H.,
            Liu,Y., Gunaratne,P., Schwartz,R.J., Burns,A.R., Kumar,A.,
            DiPersio,C.M. and Wu,M.
  TITLE     beta1 integrins regulate cellular behaviour and cardiomyocyte
            organization during ventricular wall formation
  JOURNAL   Cardiovasc Res 120 (11), 1279-1294 (2024)
   PUBMED   38794925
REFERENCE   3  (bases 1 to 5784)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5784)
  AUTHORS   Wang,L., He,L., Yi,W., Wang,M., Xu,F., Liu,H., Nie,J., Pan,Y.H.,
            Dang,S. and Zhang,W.
  TITLE     ADAMTS18-fibronectin interaction regulates the morphology of liver
            sinusoidal endothelial cells
  JOURNAL   iScience 27 (7), 110273 (2024)
   PUBMED   39040056
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5784)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 5784)
  AUTHORS   Schuger,L., Varani,J., Killen,P.D., Skubitz,A.P. and Gilbride,K.
  TITLE     Laminin expression in the mouse lung increases with development and
            stimulates spontaneous organotypic rearrangement of mixed lung
            cells
  JOURNAL   Dev Dyn 195 (1), 43-54 (1992)
   PUBMED   1292752
REFERENCE   7  (bases 1 to 5784)
  AUTHORS   Okano,R., Mita,T. and Matsui,T.
  TITLE     Characterization of a novel promoter structure and its
            transcriptional regulation of the murine laminin B1 gene
  JOURNAL   Biochim Biophys Acta 1132 (1), 49-57 (1992)
   PUBMED   1339296
REFERENCE   8  (bases 1 to 5784)
  AUTHORS   Avraham,K.B., Prezioso,V.R., Chen,W.S., Lai,E., Sladek,F.M.,
            Zhong,W., Darnell,J.E. Jr., Jenkins,N.A. and Copeland,N.G.
  TITLE     Murine chromosomal location of four hepatocyte-enriched
            transcription factors: HNF-3 alpha, HNF-3 beta, HNF-3 gamma, and
            HNF-4
  JOURNAL   Genomics 13 (2), 264-268 (1992)
   PUBMED   1612587
REFERENCE   9  (bases 1 to 5784)
  AUTHORS   Dong,L.J. and Chung,A.E.
  TITLE     The expression of the genes for entactin, laminin A, laminin B1 and
            laminin B2 in murine lens morphogenesis and eye development
  JOURNAL   Differentiation 48 (3), 157-172 (1991)
   PUBMED   1725162
REFERENCE   10 (bases 1 to 5784)
  AUTHORS   Aeschlimann,D. and Paulsson,M.
  TITLE     Cross-linking of laminin-nidogen complexes by tissue
            transglutaminase. A novel mechanism for basement membrane
            stabilization
  JOURNAL   J Biol Chem 266 (23), 15308-15317 (1991)
   PUBMED   1678389
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT571245.12 and CR974423.21.
            
            On May 24, 2019 this sequence version replaced NM_008482.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M15525.1, AK147313.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-309               CT571245.12        177351-177659
            310-485             CT571245.12        178749-178924
            486-621             CT571245.12        181115-181250
            622-695             CT571245.12        184488-184561
            696-884             CT571245.12        190520-190708
            885-948             CT571245.12        190794-190857
            949-1151            CT571245.12        194615-194817
            1152-1272           CT571245.12        197684-197804
            1273-1461           CR974423.21        235048-235236       c
            1462-1641           CR974423.21        234594-234773       c
            1642-1754           CR974423.21        234337-234449       c
            1755-1834           CR974423.21        233231-233310       c
            1835-1970           CR974423.21        224891-225026       c
            1971-2129           CR974423.21        223681-223839       c
            2130-2257           CR974423.21        222601-222728       c
            2258-2381           CR974423.21        222322-222445       c
            2382-2586           CR974423.21        221442-221646       c
            2587-2730           CR974423.21        219947-220090       c
            2731-2962           CR974423.21        219517-219748       c
            2963-3126           CR974423.21        217899-218062       c
            3127-3351           CR974423.21        216661-216885       c
            3352-3566           CR974423.21        215463-215677       c
            3567-3663           CR974423.21        214606-214702       c
            3664-4033           CR974423.21        204019-204388       c
            4034-4218           CR974423.21        202549-202733       c
            4219-4460           CR974423.21        201513-201754       c
            4461-4664           CR974423.21        198987-199190       c
            4665-4809           CR974423.21        198332-198476       c
            4810-5017           CR974423.21        196275-196482       c
            5018-5159           CR974423.21        195943-196084       c
            5160-5336           CR974423.21        194831-195007       c
            5337-5496           CR974423.21        193383-193542       c
            5497-5784           CR974423.21        193008-193295       c
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 13.39 cM"
     gene            1..5784
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /note="laminin B1"
                     /db_xref="GeneID:16777"
                     /db_xref="MGI:MGI:96743"
     exon            1..309
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             273..5633
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /note="laminin subunit beta-1; laminin B1 chain; laminin-1
                     subunit beta; laminin-2 subunit beta; laminin-6 subunit
                     beta; laminin-8 subunit beta; laminin-10 subunit beta;
                     laminin-12 subunit beta; laminin B1 subunit 1;
                     laminin-111"
                     /codon_start=1
                     /product="laminin subunit beta-1 precursor"
                     /protein_id="NP_032508.3"
                     /db_xref="CCDS:CCDS25862.2"
                     /db_xref="GeneID:16777"
                     /db_xref="MGI:MGI:96743"
                     /translation="MGLLQVFAFGVLALWGTRVCAQEPEFSYGCAEGSCYPATGDLLI
                     GRAQKLSVTSTCGLHKPEPYCIVSHLQEDKKCFICDSRDPYHETLNPDSHLIENVVTT
                     FAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAW
                     GVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIED
                     PYSPRIQNLLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGH
                     ASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKK
                     CNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDP
                     NLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAE
                     DPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSNDLDGCR
                     PCDCDLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGP
                     GVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHW
                     EKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRL
                     ELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCL
                     ENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNV
                     VGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLP
                     GYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHC
                     RPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGS
                     CQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVC
                     NYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNA
                     AHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDVECRACDCDPRGIETPQCDQ
                     STGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKA
                     KALKISGVIGPYRETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTE
                     KMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDS
                     ITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRVEDLMLERESPFKEQQEEQARLL
                     DELAGKLQSLDLSAVAQMTCGTPPGADCSESECGGPNCRTDEGEKKCGGPGCGGLVTV
                     AHSAWQKAMDFDRDVLSALAEVEQLSKMVSEAKVRADEAKQNAQDVLLKTNATKEKVD
                     KSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVE
                     TLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAE
                     KAIKQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGE
                     AEYIEKVVYSVKQNADDVKKTLDGELDEKYKKVESLIAQKTEESADARRKAELLQNEA
                     KTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRSLLKDISEKVAVYST
                     CL"
     exon            310..485
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            486..621
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            622..695
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            696..884
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            885..948
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1151
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1272
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1461
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1641
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1754
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1834
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..1970
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2129
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2257
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2381
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2586
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2730
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2962
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2963..3126
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3351
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3566
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3567..3663
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3664..4033
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4034..4218
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4219..4460
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4461..4664
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4665..4809
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4810..5017
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5018..5159
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5160..5336
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5337..5496
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5497..5784
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5757..5762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /note="hexamer: AATAAA"
     polyA_site      5784
                     /gene="Lamb1"
                     /gene_synonym="D130003D08Rik; Lamb-1; Lamb1-1"
                     /note="major polyA site"
ORIGIN      
        1 cttctttggg ccggtgggct gccaggagcg ggttgagcgc tcgcccagcc gggtgaggag
       61 aacaaagtag ttaagcgact tgaccccctt cctgggccca gcccccgctt ccgtgggagc
      121 ggcaggaaat ggaagggccc ctctcctctc tcccaacatt tgccttttct ccccgctacc
      181 tctccagaaa ggaagacccg aagaaaagac aggcagcttg cctgctgcgt cctccttccc
      241 gtgccgcgtc ccctcgtctg cgaggactgg acatggggct gctccaggtg ttcgcctttg
      301 gtgtcctagc cctatggggc acccgagtgt gcgctcagga accggagttc agctatggct
      361 gcgcagaagg cagctgctac cctgccactg gcgaccttct catcggccga gcgcaaaagc
      421 tctccgtgac ttcgacatgt ggactgcaca aaccagagcc ctactgtatt gttagccacc
      481 tgcaggagga caagaaatgc ttcatatgtg actcccgaga cccttatcac gagaccctca
      541 accccgacag ccatctcatt gagaacgtgg tcaccacatt tgctccaaac cgccttaaga
      601 tctggtggca atcggaaaat ggtgtggaga acgtgaccat ccaactggac ctggaagcag
      661 aattccattt cactcatctc atcatgacct tcaagacatt ccgcccagcc gccatgctga
      721 tcgagcggtc ttctgacttt gggaaggctt ggggcgtgta cagatacttc gcctacgact
      781 gtgagagctc gttcccaggc atttcaactg gacccatgaa gaaagtggat gacatcatct
      841 gtgactctcg atattctgac attgagccct cgacagaagg agaggtaata tttcgtgctt
      901 tagatcctgc tttcaaaatt gaagaccctt atagtccaag gatacagaat ctattaaaaa
      961 tcaccaactt gagaatcaag tttgtgaaac tgcacacctt gggggataac cttttggact
     1021 ccagaatgga aatccgagag aagtactatt acgctgttta tgatatggtg gttcgaggga
     1081 actgcttctg ctatggccac gccagtgaat gcgcccctgt ggatggagtc aatgaagaag
     1141 tggaaggaat ggttcacggg cactgcatgt gcagacacaa caccaaaggc ctgaactgtg
     1201 agctgtgcat ggatttctac cacgatttgc cgtggagacc tgctgaaggc cggaacagca
     1261 acgcctgcaa aaaatgtaac tgcaatgaac attccagctc gtgtcacttt gacatggcag
     1321 tcttcctggc tactggcaac gtcagcgggg gagtgtgtga taactgtcag cacaacacca
     1381 tggggcgcaa ctgtgaacag tgcaaaccgt tctacttcca gcaccctgag agggacatcc
     1441 gggaccccaa tctctgtgaa ccatgtacct gtgacccagc tggttctgag aatggcggga
     1501 tctgtgatgg gtacactgat ttttctgtgg gtctcattgc tggtcagtgt cggtgcaaat
     1561 tgcacgtgga gggagagcgc tgtgatgttt gtaaagaagg cttctacgac ttaagtgctg
     1621 aagacccgta tggttgtaaa tcatgtgctt gcaatcctct gggaacaatt cctggtggga
     1681 atccttgtga ttctgagact ggctactgct actgtaagcg cctggtgaca ggacagcgct
     1741 gtgaccagtg cctgccgcag cactggggtt taagcaatga tttggatggg tgtcgacctt
     1801 gtgactgtga ccttggaggg gcgctgaaca atagctgctc cgaggactcc ggccagtgct
     1861 cctgcctgcc ccacatgatt gggcggcagt gtaacgaggt ggagtccggt tactacttca
     1921 ccaccctgga ccactacatc tacgaagccg aggaagccaa tctggggcct ggagtcattg
     1981 tggtggaaag gcagtacatt caggaccgca ttccttcctg gacaggacct gggttcgtcc
     2041 gggtgcctga aggggcttat ttggagtttt tcattgacaa cataccgtat tccatggagt
     2101 atgaaatcct gattcgctat gagccacagc tgccggacca ctgggagaaa gctgtcatca
     2161 ctgtacagcg gccggggaag attccagcca gcagccgatg tggtaacacc gttcccgatg
     2221 atgacaacca ggtggtgtcc ttgtcaccgg gctcaagata cgttgtcctc cctcgccccg
     2281 tgtgctttga gaagggaatg aactacacgg tgaggttgga gctgccccag tatacggcat
     2341 cgggcagtga cgtggagagc ccttacacgt tcatcgactc gcttgttctc atgccctact
     2401 gtaaatcgct ggacatcttc actgttggcg gctcaggcga tggggaggtc accaatagtg
     2461 cctgggaaac cttccagcgc tacaggtgtc tggagaacag caggagtgtg gtaaaaacac
     2521 ccatgacaga tgtctgcaga aacattatct tcagcatttc tgccttgatt caccagacgg
     2581 gccttgcttg tgaatgtgac ccccagggat ctctgagttc tgtgtgtgac cccaatggtg
     2641 gccagtgcca gtgccgtcct aatgtggttg gaagaacctg caacaggtgt gccccgggca
     2701 cctttggctt tggccccaac ggatgcaaac cttgtgactg ccatctgcaa gggtctgcca
     2761 gtgccttctg cgatgcgatc actggccagt gccactgttt ccagggcatc tatgctcggc
     2821 agtgtgaccg atgtctccct gggtattggg gctttcccag ctgccagccc tgccagtgta
     2881 atggtcatgc tctagactgt gacacagtga caggggagtg tctgagctgt caggactaca
     2941 ccacgggcca caactgcgaa aggtgcctgg ctggctacta cggtgatccc atcattgggt
     3001 caggagacca ctgtcgccct tgcccttgtc ctgatggtcc tgacagtgga cgacagtttg
     3061 ccaggagctg ttatcaagac cccgtcactc tccagcttgc gtgtgtttgt gatcctgggt
     3121 acattggctc cagatgtgat gactgtgcct ctggattttt tggcaatccc tcagactttg
     3181 ggggttcatg tcaaccatgt cagtgccacc acaacattga cactaccgat ccagaagcct
     3241 gtgacaagga gacgggacga tgcctcaagt gcctgtacca cacagaaggg gaccattgcc
     3301 agctctgcca gtatgggtac tacggcgatg ctcttcggca agactgtaga aagtgtgtct
     3361 gcaattacct gggcacggtg aaggaacatt gtaatggctc tgactgccac tgtgacaaag
     3421 ccactggtca gtgctcgtgc cttcccaatg tgatcgggca gaactgtgac cggtgtgcgc
     3481 ccaacacctg gcagctggct agcgggactg gctgcgggcc ctgcaattgc aatgctgcgc
     3541 attcctttgg gccatcctgc aacgagttca cagggcagtg ccagtgcatg ccgggctttg
     3601 gaggccgaac ctgcagcgag tgccaggagc tcttctgggg agaccctgat gtggaatgcc
     3661 gagcctgtga ctgtgatccc aggggcattg agacacctca gtgtgaccag tccacgggcc
     3721 agtgtgtctg tgtggagggt gtagagggtc ctcgctgcga caagtgcacc agaggttact
     3781 cgggggtctt tcctgactgc acaccctgcc accagtgctt tgctctctgg gatgctatca
     3841 ttggtgagct gaccaacagg acccacaaat tcctggagaa agccaaggct ctgaaaatca
     3901 gtggtgtgat tggtccctac cgagagaccg tggactctgt agagaagaaa gtcaatgaga
     3961 taaaagacat cctggcccag agcccagcag cggaaccact gaaaaacatt ggcattctct
     4021 tcgaggaggc agagaaacta accaaagatg tcacagaaaa gatggcgcag gtagaagtga
     4081 aattaactga tacagcttca cagagtaaca gcacagctgg agagctcggc gcactgcagg
     4141 cagaagcaga gagccttgac aagaccgtga aggagctggc agaacagctg gagtttatca
     4201 aaaactccga tattcagggc gccttggata gcatcaccaa gtatttccag atgtctcttg
     4261 aggcagagaa gcgggtgaat gcctccacca cagaccccaa cagcactgtg gagcagtctg
     4321 ccctcacgcg agacagagta gaagatctga tgttggagcg agagtctccg ttcaaggagc
     4381 agcaggagga acaggcacgc ctcctggacg aactggccgg caaactgcaa agtctcgacc
     4441 tgtcggctgt tgcacagatg acctgtggaa cacctccagg ggctgactgt tctgaaagtg
     4501 aatgtggtgg ccccaactgc agaactgacg aaggagagaa gaagtgtggg gggcctggct
     4561 gtggtggtct ggtcactgtg gcccacagtg cttggcagaa agccatggat tttgaccgtg
     4621 atgtcctgag tgccctggct gaagtcgaac agctctccaa gatggtctct gaagcaaaag
     4681 tgagagcaga tgaggcgaag cagaatgcgc aggatgtcct gttaaaaaca aatgctacca
     4741 aagaaaaagt ggacaagagc aacgaggacc tgcggaacct catcaagcag atcagaaact
     4801 tcctgactga ggatagtgct gatctagaca gtattgaagc agttgctaat gaagtactga
     4861 aaatggaaat gcctagcacg ccacagcagt tacagaacct aacagaagac attcgggagc
     4921 gagttgaaac cctctctcaa gtagaggtta ttttgcagca gagtgcagct gacattgcca
     4981 gagctgagct gttgcttgag gaagctaaga gagcaagcaa aagtgcaaca gatgttaaag
     5041 tcactgcaga catggtgaag gaagcattag aagaagcaga aaaggcccag gttgcagcag
     5101 agaaggcgat taaacaagct gatgaggata tccaaggaac ccaaaacctg ctaacatcga
     5161 ttgaatctga aacggcagct tctgaggaaa ccctgaccaa cgcctcccag cgcatcagca
     5221 agcttgagag gaacgtggaa gagcttaagc gtaaagctgc ccagaactct ggggaggcag
     5281 aatatatcga aaaagtagta tattctgtaa aacagaatgc agacgatgtt aagaagactc
     5341 tagatggcga acttgatgaa aagtataaga aggtagaaag tttaattgcc caaaaaactg
     5401 aagagtcagc agatgccagg aggaaagctg agctgctaca aaatgaagca aaaacactct
     5461 tggctcaagc taacagcaag ctccagctgt tggaagactt agaaagaaaa tatgaggaca
     5521 atcaaaaata cttagaagat aaagctcaag aattggtgcg actggaagga gaggttcgct
     5581 ccctccttaa ggacataagt gagaaagttg cggtttacag cacctgctta taacaggaag
     5641 gggctgtaga ggggctcggt gaccaaggta aaccacacgc gcaaaccgag gcagtcatct
     5701 acaaataacc catcatctat ttaatgtttt taaccaccta cttttgtatg gagttaaata
     5761 aaagacattg gttttgtata aaca
//
