LOCUS       NM_027552               2740 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus kynureninase (Kynu), transcript variant 1, mRNA.
ACCESSION   NM_027552
VERSION     NM_027552.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Wan,J., Cheng,C., Hu,J., Huang,H., Han,Q., Jie,Z., Zou,Q., Shi,J.H.
            and Yu,X.
  TITLE     De novo NAD+ synthesis contributes to CD8+ T cell metabolic fitness
            and antitumor function
  JOURNAL   Cell Rep 42 (12), 113518 (2023)
   PUBMED   38041812
REFERENCE   3  (bases 1 to 2740)
  AUTHORS   Kesarwani,P., Kant,S., Zhao,Y., Prabhu,A., Buelow,K.L., Miller,C.R.
            and Chinnaiyan,P.
  TITLE     Quinolinate promotes macrophage-induced immune tolerance in
            glioblastoma through the NMDAR/PPARgamma signaling axis
  JOURNAL   Nat Commun 14 (1), 1459 (2023)
   PUBMED   36927729
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2740)
  AUTHORS   Wang,M., Wang,Y., Zhang,M., Duan,Q., Chen,C., Sun,Q., Liu,M.,
            Zheng,Y. and Shao,Y.
  TITLE     Kynureninase contributes to the pathogenesis of psoriasis through
            pro-inflammatory effect
  JOURNAL   J Cell Physiol 237 (1), 1044-1056 (2022)
   PUBMED   34553380
  REMARK    GeneRIF: Kynureninase contributes to the pathogenesis of psoriasis
            through pro-inflammatory effect.
REFERENCE   5  (bases 1 to 2740)
  AUTHORS   Shi,H., Enriquez,A., Rapadas,M., Martin,E.M.M.A., Wang,R.,
            Moreau,J., Lim,C.K., Szot,J.O., Ip,E., Hughes,J.N., Sugimoto,K.,
            Humphreys,D.T., McInerney-Leo,A.M., Leo,P.J., Maghzal,G.J.,
            Halliday,J., Smith,J., Colley,A., Mark,P.R., Collins,F.,
            Sillence,D.O., Winlaw,D.S., Ho,J.W.K., Guillemin,G.J., Brown,M.A.,
            Kikuchi,K., Thomas,P.Q., Stocker,R., Giannoulatou,E., Chapman,G.,
            Duncan,E.L., Sparrow,D.B. and Dunwoodie,S.L.
  TITLE     NAD Deficiency, Congenital Malformations, and Niacin
            Supplementation
  JOURNAL   N Engl J Med 377 (6), 544-552 (2017)
   PUBMED   28792876
REFERENCE   6  (bases 1 to 2740)
  AUTHORS   Ito,M., Ogawa,K., Takeuchi,K., Nakada,A., Heishi,M., Suto,H.,
            Mitsuishi,K., Sugita,Y., Ogawa,H. and Ra,C.
  TITLE     Gene expression of enzymes for tryptophan degradation pathway is
            upregulated in the skin lesions of patients with atopic dermatitis
            or psoriasis
  JOURNAL   J Dermatol Sci 36 (3), 157-164 (2004)
   PUBMED   15541637
  REMARK    GeneRIF: Levels of mRNA for IDO (indoleamine 2,3-dioxygenase) and
            kynureninase, enzymes constituting the tryptophan degradation
            pathway, were found to be upregulated in the skin lesions of atopic
            dermatitis or psoriasis
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845336.9 and AL845160.8.
            
            On Dec 21, 2021 this sequence version replaced NM_027552.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK014484.1, AK050024.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                AL845336.9         73795-73875
            82-263              AL845336.9         81423-81604
            264-384             AL845336.9         99812-99932
            385-467             AL845336.9         107855-107937
            468-529             AL845336.9         118047-118108
            530-601             AL845336.9         119191-119262
            602-676             AL845336.9         120709-120783
            677-823             AL845336.9         122608-122754
            824-922             AL845336.9         147419-147517
            923-996             AL845336.9         150012-150085
            997-1049            AL845160.8         15047-15099
            1050-1135           AL845160.8         16359-16444
            1136-1366           AL845160.8         23440-23670
            1367-2740           AL845160.8         25037-26410
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 25.64 cM"
     gene            1..2740
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /note="kynureninase"
                     /db_xref="GeneID:70789"
                     /db_xref="MGI:MGI:1918039"
     exon            1..81
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            82..263
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..1489
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /EC_number="3.7.1.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     L-kynurenine hydrolase"
                     /codon_start=1
                     /product="kynureninase isoform 1"
                     /protein_id="NP_081828.2"
                     /db_xref="CCDS:CCDS16018.1"
                     /db_xref="GeneID:70789"
                     /db_xref="MGI:MGI:1918039"
                     /translation="MEPSPLELPVDAVRRIAAELNCDPTDERVALRLDEEDKLSHFRN
                     CFYIPKMRDLPSIDLSLVSEDDDAIYFLGNSLGLQPKMVRTYLEEELDKWAKMGAYGH
                     DVGKRPWIVGDESIVSLMKDIVGAHEKEIALMNALTINLHLLLLSFFKPTPKRHKILL
                     EAKAFPSDHYAIESQIQLHGLDVEKSMRMVKPREGEETLRMEDILEVIEEEGDSIAVI
                     LFSGLHFYTGQLFNIPAITKAGHAKGCFVGFDLAHAVGNVELRLHDWGVDFACWCSYK
                     YLNSGAGGLAGAFVHEKHAHTVKPALVGWFGHDLSTRFNMDNKLQLIPGANGFRISNP
                     PILLVCSLHASLEVFQQATMTALRRKSILLTGYLEYMLKHYHSKDNTENKGPIVNIIT
                     PSRAEERGCQLTLTFSIPKKSVFKELEKRGVVCDKREPDGIRVAPVPLYNSFHDVYKF
                     IRLLTSILDSSERS"
     exon            264..384
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            385..467
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            468..529
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            530..601
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            602..676
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            677..823
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            824..922
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            923..996
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1049
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1135
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1366
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..2740
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2714..2719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2740
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /note="major polyA site"
ORIGIN      
        1 agttctttgg ctagctgggg acaaagaaag atccagcatc ctctgagaag gtactgaaga
       61 ctactgtctg gatctgagca gataacagtt tatgatggag ccttcgcctc ttgagcttcc
      121 agttgatgca gtgcggcgca tcgcggctga actcaattgt gacccaacag atgagagggt
      181 tgctctccgc ttggatgagg aagataaact gagtcatttt aggaactgtt tttatattcc
      241 caaaatgcgg gacctgcctt caattgatct atctttagtg agtgaggatg atgatgccat
      301 ctatttcctg ggaaattccc ttggccttca accgaaaatg gttaggacat acctggagga
      361 agaactagat aagtgggcca agatgggagc ctatggccat gatgtaggca aacgcccttg
      421 gattgtaggg gatgagagta ttgtaagcct tatgaaggac attgtaggag cccatgagaa
      481 agaaatagct ctaatgaatg ctttgactat taatttacat ctcctgctgt tatcattctt
      541 taagcctact ccaaagcggc acaaaattct tctagaagcc aaagccttcc cttctgatca
      601 ttatgctatt gagtcacaga ttcaacttca cggacttgat gttgagaaaa gtatgcggat
      661 ggtaaagcca cgagaggggg aagagacctt aaggatggag gacatactgg aagtaatcga
      721 ggaggaagga gactcgatcg ccgtgatcct gttcagtggg ctgcactttt atactggaca
      781 gctgttcaac attcctgcca taacaaaagc tggacatgca aagggctgtt ttgttggctt
      841 tgacctagca catgcagttg gaaatgttga actccgctta catgactggg gtgttgactt
      901 tgcctgctgg tgttcctata agtatttaaa ttcaggagct ggaggtctgg ctggtgcctt
      961 tgtccacgag aaacatgctc atactgtcaa gcctgcgtta gtgggatggt tcggccatga
     1021 cctcagtaca aggtttaaca tggataacaa actacaatta atccccgggg ccaatggatt
     1081 ccgaatttca aaccctccca ttttgttggt ctgctccttg cacgccagtt tagaggtctt
     1141 tcagcaagca actatgactg cgctgagaag aaaatccatt ctgctgacag gttatctgga
     1201 atacatgctc aaacattacc acagcaaaga taacaccgaa aacaaggggc cgattgtgaa
     1261 tatcatcacc ccgtccagag cagaggagcg tggctgccag ttaacactca ccttttccat
     1321 tcccaagaaa agcgttttta aggaactaga aaaaagagga gtcgtttgtg acaagcgaga
     1381 accagatggc atccgcgtgg cccctgttcc tctctataat tctttccatg atgtttataa
     1441 gttcatcaga ctgctcactt ccatactcga ctcttcagaa agaagctagc tatattttct
     1501 agcacaactc aagtaaatct cactgaaagg tgatggagtt ttcacttcta ttgaatttta
     1561 gtcattaaaa aaatctccag aaattgattg cacagaaatg ataactataa aaaaatttac
     1621 ataaaacctg gtgcatgctt taatatctgt gtttctgggg aacgtggtgt cctgtgaatt
     1681 atgaagtcac actttacatg actacagcct acagatgact gtcttgatca gttgtcacat
     1741 ttcatgctca ctgaaacatt ttctctttaa tttgtgactg aatttccaac gttataatgt
     1801 atatggactt cttgtataaa tattagaagt attactttaa ttttgctata gagttttatt
     1861 ttaatatttg taactgaatc atctgaaata tgtttgatat gatcatgttt tatctaattc
     1921 caggagggga acagcctttt aagctgttac aaaatctctc tctctctctc tctctctctc
     1981 tctctctctc tctctctctc tctctctctc tctctctttc ccccccccag tggtgtgtgt
     2041 gtctatgtgt ttgtgtttct gtgtgtctgt gtaaaggaca tgtaagtgct tatgtataag
     2101 ggatgaggta cttgacccta tgtactcttg tgaggccaga ggtcaacact ggacatcttc
     2161 ctcaatcact gtttaaaatt ttatttattt atttattttt atgtgtatgg gtattttgac
     2221 tgcatttatg tctgtacctc atgtgcatgc catgattaca gaaactagaa gatacatcag
     2281 atcacctgag actggagtta cagagctgct gtgtggatac taggaattga acccaggtcg
     2341 tttggaagaa tagccaacgc tcttattctt tgacacatct ctccagcctt tccacttcat
     2401 atttcaatac atgatttctc cccaaacctg gaacttgctc cttcagctcg tgtggctggc
     2461 cagtgagtct tcagcgtttc tctgtctctg ccctacaatg aatgcgggtt acagctgtac
     2521 actgttgcac atagattttt tacatgtcta ctgtgatctg aacacagtcc ttatatcagt
     2581 tcagcaacca cttcatcgac caagcaatcc cccagtcatt gcttttttga tgccactact
     2641 agtatgcatt tactggcaaa gaattctaag tttgtatgta gaaagaaaaa gttataatga
     2701 tttgataaac ttgaataaaa catacttggt cagacagaaa
//
