LOCUS       NM_001289593            1604 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus kynureninase (Kynu), transcript variant 2, mRNA.
ACCESSION   NM_001289593
VERSION     NM_001289593.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wan,J., Cheng,C., Hu,J., Huang,H., Han,Q., Jie,Z., Zou,Q., Shi,J.H.
            and Yu,X.
  TITLE     De novo NAD+ synthesis contributes to CD8+ T cell metabolic fitness
            and antitumor function
  JOURNAL   Cell Rep 42 (12), 113518 (2023)
   PUBMED   38041812
REFERENCE   3  (bases 1 to 1604)
  AUTHORS   Kesarwani,P., Kant,S., Zhao,Y., Prabhu,A., Buelow,K.L., Miller,C.R.
            and Chinnaiyan,P.
  TITLE     Quinolinate promotes macrophage-induced immune tolerance in
            glioblastoma through the NMDAR/PPARgamma signaling axis
  JOURNAL   Nat Commun 14 (1), 1459 (2023)
   PUBMED   36927729
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1604)
  AUTHORS   Wang,M., Wang,Y., Zhang,M., Duan,Q., Chen,C., Sun,Q., Liu,M.,
            Zheng,Y. and Shao,Y.
  TITLE     Kynureninase contributes to the pathogenesis of psoriasis through
            pro-inflammatory effect
  JOURNAL   J Cell Physiol 237 (1), 1044-1056 (2022)
   PUBMED   34553380
  REMARK    GeneRIF: Kynureninase contributes to the pathogenesis of psoriasis
            through pro-inflammatory effect.
REFERENCE   5  (bases 1 to 1604)
  AUTHORS   Shi,H., Enriquez,A., Rapadas,M., Martin,E.M.M.A., Wang,R.,
            Moreau,J., Lim,C.K., Szot,J.O., Ip,E., Hughes,J.N., Sugimoto,K.,
            Humphreys,D.T., McInerney-Leo,A.M., Leo,P.J., Maghzal,G.J.,
            Halliday,J., Smith,J., Colley,A., Mark,P.R., Collins,F.,
            Sillence,D.O., Winlaw,D.S., Ho,J.W.K., Guillemin,G.J., Brown,M.A.,
            Kikuchi,K., Thomas,P.Q., Stocker,R., Giannoulatou,E., Chapman,G.,
            Duncan,E.L., Sparrow,D.B. and Dunwoodie,S.L.
  TITLE     NAD Deficiency, Congenital Malformations, and Niacin
            Supplementation
  JOURNAL   N Engl J Med 377 (6), 544-552 (2017)
   PUBMED   28792876
REFERENCE   6  (bases 1 to 1604)
  AUTHORS   Ito,M., Ogawa,K., Takeuchi,K., Nakada,A., Heishi,M., Suto,H.,
            Mitsuishi,K., Sugita,Y., Ogawa,H. and Ra,C.
  TITLE     Gene expression of enzymes for tryptophan degradation pathway is
            upregulated in the skin lesions of patients with atopic dermatitis
            or psoriasis
  JOURNAL   J Dermatol Sci 36 (3), 157-164 (2004)
   PUBMED   15541637
  REMARK    GeneRIF: Levels of mRNA for IDO (indoleamine 2,3-dioxygenase) and
            kynureninase, enzymes constituting the tryptophan degradation
            pathway, were found to be upregulated in the skin lesions of atopic
            dermatitis or psoriasis
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK149423.1, AK050024.1 and AL845160.8.
            
            Transcript Variant: This variant (2) contains a 3' terminal exon
            which extends past a splice site that is used in variant 1. This
            results in a novel 3' coding region and 3' UTR, compared to variant
            1. It encodes isoform 2 which is shorter and has a distinct
            C-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC069848.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AK149423.1         1-4
            5-1370              AK050024.1         2-1367
            1371-1604           AL845160.8         23671-23904
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 25.64 cM"
     gene            1..1604
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /note="kynureninase"
                     /db_xref="GeneID:70789"
                     /db_xref="MGI:MGI:1918039"
     exon            1..85
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            86..267
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1382
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /EC_number="3.7.1.3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     L-kynurenine hydrolase"
                     /codon_start=1
                     /product="kynureninase isoform 2"
                     /protein_id="NP_001276522.1"
                     /db_xref="CCDS:CCDS79792.1"
                     /db_xref="GeneID:70789"
                     /db_xref="MGI:MGI:1918039"
                     /translation="MMEPSPLELPVDAVRRIAAELNCDPTDERVALRLDEEDKLSHFR
                     NCFYIPKMRDLPSIDLSLVSEDDDAIYFLGNSLGLQPKMVRTYLEEELDKWAKMGAYG
                     HDVGKRPWIVGDESIVSLMKDIVGAHEKEIALMNALTINLHLLLLSFFKPTPKRHKIL
                     LEAKAFPSDHYAIESQIQLHGLDVEKSMRMVKPREGEETLRMEDILEVIEEEGDSIAV
                     ILFSGLHFYTGQLFNIPAITKAGHAKGCFVGFDLAHAVGNVELRLHDWGVDFACWCSY
                     KYLNSGAGGLAGAFVHEKHAHTVKPALVGWFGHDLSTRFNMDNKLQLIPGANGFRISN
                     PPILLVCSLHASLEVFQQATMTALRRKSILLTGYLEYMLKHYHSKDNTENKGPIVNII
                     TPSRAEERGCQLTLTFSIPKKSVFKELEKRGVVVCI"
     exon            268..388
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            389..471
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            472..533
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            534..605
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            606..680
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            681..827
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            828..926
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1000
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1053
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1139
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1604
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1574..1579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1604
                     /gene="Kynu"
                     /gene_synonym="4432411A05Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagcagttct ttggctagct ggggacaaag aaagatccag catcctctga gaaggtactg
       61 aagactactg tctggatctg agcagataac agtttatgat ggagccttcg cctcttgagc
      121 ttccagttga tgcagtgcgg cgcatcgcgg ctgaactcaa ttgtgaccca acagatgaga
      181 gggttgctct ccgcttggat gaggaagata aactgagtca ttttaggaac tgtttttata
      241 ttcccaaaat gcgggacctg ccttcaattg atctatcttt agtgagtgag gatgatgatg
      301 ccatctattt cctgggaaat tcccttggcc ttcaaccgaa aatggttagg acatacctgg
      361 aggaagaact agataagtgg gccaagatgg gagcctatgg ccatgatgta ggcaaacgcc
      421 cttggattgt aggggatgag agtattgtaa gccttatgaa ggacattgta ggagcccatg
      481 agaaagaaat agctctaatg aatgctttga ctattaattt acatctcctg ctgttatcat
      541 tctttaagcc tactccaaag cggcacaaaa ttcttctaga agccaaagcc ttcccttctg
      601 atcattatgc tattgagtca cagattcaac ttcacggact tgatgttgag aaaagtatgc
      661 ggatggtaaa gccacgagag ggggaagaga ccttaaggat ggaggacata ctggaagtaa
      721 tcgaggagga aggagactcg atcgccgtga tcctgttcag tgggctgcac ttttatactg
      781 gacagctgtt caacattcct gccataacaa aagctggaca tgcaaagggc tgttttgttg
      841 gctttgacct agcacatgca gttggaaatg ttgaactccg cttacatgac tggggtgttg
      901 actttgcctg ctggtgttcc tataagtatt taaattcagg agctggaggt ctggctggtg
      961 cctttgtcca cgagaaacat gctcatactg tcaagcctgc gttagtggga tggttcggcc
     1021 atgacctcag tacaaggttt aacatggata acaaactaca attaatcccc ggggccaatg
     1081 gattccgaat ttcaaaccct cccattttgt tggtctgctc cttgcacgcc agtttagagg
     1141 tctttcagca agcaactatg actgcgctga gaagaaaatc cattctgctg acaggttatc
     1201 tggaatacat gctcaaacat taccacagca aagataacac cgaaaacaag gggccgattg
     1261 tgaatatcat caccccgtcc agagcagagg agcgtggctg ccagttaaca ctcacctttt
     1321 ccattcccaa gaaaagcgtt tttaaggaac tagaaaaaag aggagtcgtt gtatgtatat
     1381 aaatttatgt cttgcatttc accacgcttt ctctctgggt tccaggttgg gatgagtctt
     1441 aggttactaa acttatcctt ttattgcata tccttctagt caaccaatca agacaagtca
     1501 gacatcaact tagcactcag cgttagccct gatggatggc tacaagagcc catggtcatt
     1561 tctgatcaaa gggaataaat agcaaatctt gcttttgaaa aata
//
