LOCUS       NM_001379493            4420 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus kinectin 1 (Ktn1), transcript variant 19, mRNA.
ACCESSION   NM_001379493 XM_006518640
VERSION     NM_001379493.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Valnegri,P., Huang,J., Yamada,T., Yang,Y., Mejia,L.A., Cho,H.Y.,
            Oldenborg,A. and Bonni,A.
  TITLE     RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the
            mammalian brain
  JOURNAL   Nat Commun 8 (1), 1271 (2017)
   PUBMED   29097665
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Wei,N., Cheng,Y., Wang,Z., Liu,Y., Luo,C., Liu,L., Chen,L., Xie,Z.,
            Lu,Y. and Feng,Y.
  TITLE     SRSF10 Plays a Role in Myoblast Differentiation and Glucose
            Production via Regulation of Alternative Splicing
  JOURNAL   Cell Rep 13 (8), 1647-1657 (2015)
   PUBMED   26586428
REFERENCE   3  (bases 1 to 4420)
  AUTHORS   Yates,L.L., Papakrivopoulou,J., Long,D.A., Goggolidou,P.,
            Connolly,J.O., Woolf,A.S. and Dean,C.H.
  TITLE     The planar cell polarity gene Vangl2 is required for mammalian
            kidney-branching morphogenesis and glomerular maturation
  JOURNAL   Hum Mol Genet 19 (23), 4663-4676 (2010)
   PUBMED   20843830
REFERENCE   4  (bases 1 to 4420)
  AUTHORS   Kalsotra,A., Xiao,X., Ward,A.J., Castle,J.C., Johnson,J.M.,
            Burge,C.B. and Cooper,T.A.
  TITLE     A postnatal switch of CELF and MBNL proteins reprograms alternative
            splicing in the developing heart
  JOURNAL   Proc Natl Acad Sci U S A 105 (51), 20333-20338 (2008)
   PUBMED   19075228
REFERENCE   5  (bases 1 to 4420)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   6  (bases 1 to 4420)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 4420)
  AUTHORS   Plitz,T. and Pfeffer,K.
  TITLE     Intact lysosome transport and phagosome function despite kinectin
            deficiency
  JOURNAL   Mol Cell Biol 21 (17), 6044-6055 (2001)
   PUBMED   11486041
REFERENCE   8  (bases 1 to 4420)
  AUTHORS   Alberts,A.S., Bouquin,N., Johnston,L.H. and Treisman,R.
  TITLE     Analysis of RhoA-binding proteins reveals an interaction domain
            conserved in heterotrimeric G protein beta subunits and the yeast
            response regulator protein Skn7
  JOURNAL   J Biol Chem 273 (15), 8616-8622 (1998)
   PUBMED   9535835
REFERENCE   9  (bases 1 to 4420)
  AUTHORS   Morris,C., McDonald,M., Leung,E., Weaver,A. and Krissansen,G.W.
  TITLE     Assignment of the murine kinectin gene (Ktn1) to mouse chromosome
            14C2 by fluorescence in situ hybridization
  JOURNAL   Cytogenet Cell Genet 81 (1), 87-88 (1998)
   PUBMED   9691184
REFERENCE   10 (bases 1 to 4420)
  AUTHORS   Leung,E., Print,C.G., Parry,D.A., Closey,D.N., Lockhart,P.J.,
            Skinner,S.J., Batchelor,D.C. and Krissansen,G.W.
  TITLE     Cloning of novel kinectin splice variants with alternative
            C-termini: structure, distribution and evolution of mouse kinectin
  JOURNAL   Immunol Cell Biol 74 (5), 421-433 (1996)
   PUBMED   8912005
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT009515.16.
            
            On Apr 6, 2020 this sequence version replaced XM_006518640.4.
            
            Transcript Variant: Note that isoforms 19 and 22 are the same
            length but differ in aa sequence.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.206728.1,
                                           SRR1660815.72919.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849390
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               CT009515.16        182172-182300       c
            130-689             CT009515.16        167296-167855       c
            690-827             CT009515.16        164178-164315       c
            828-1001            CT009515.16        161313-161486       c
            1002-1132           CT009515.16        158541-158671       c
            1133-1249           CT009515.16        156745-156861       c
            1250-1390           CT009515.16        154786-154926       c
            1391-1497           CT009515.16        148122-148228       c
            1498-1630           CT009515.16        146758-146890       c
            1631-1718           CT009515.16        145268-145355       c
            1719-1885           CT009515.16        141496-141662       c
            1886-1954           CT009515.16        140634-140702       c
            1955-1992           CT009515.16        139135-139172       c
            1993-2083           CT009515.16        138117-138207       c
            2084-2152           CT009515.16        137668-137736       c
            2153-2190           CT009515.16        136964-137001       c
            2191-2272           CT009515.16        136767-136848       c
            2273-2341           CT009515.16        136040-136108       c
            2342-2376           CT009515.16        130957-130991       c
            2377-2467           CT009515.16        130452-130542       c
            2468-2536           CT009515.16        129868-129936       c
            2537-2574           CT009515.16        128445-128482       c
            2575-2665           CT009515.16        127334-127424       c
            2666-2734           CT009515.16        127123-127191       c
            2735-2772           CT009515.16        125811-125848       c
            2773-2863           CT009515.16        125113-125203       c
            2864-2901           CT009515.16        124332-124369       c
            2902-2992           CT009515.16        122719-122809       c
            2993-3052           CT009515.16        120710-120769       c
            3053-3087           CT009515.16        119103-119137       c
            3088-3178           CT009515.16        116889-116979       c
            3179-3265           CT009515.16        114651-114737       c
            3266-3355           CT009515.16        111326-111415       c
            3356-3436           CT009515.16        107561-107641       c
            3437-3526           CT009515.16        106870-106959       c
            3527-3619           CT009515.16        106641-106733       c
            3620-3691           CT009515.16        105611-105682       c
            3692-3775           CT009515.16        105302-105385       c
            3776-3859           CT009515.16        101188-101271       c
            3860-3955           CT009515.16        98289-98384         c
            3956-4420           CT009515.16        95047-95511         c
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 24.6 cM"
     gene            1..4420
                     /gene="Ktn1"
                     /note="kinectin 1"
                     /db_xref="GeneID:16709"
                     /db_xref="MGI:MGI:109153"
     exon            1..129
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            130..689
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..3991
                     /gene="Ktn1"
                     /note="isoform 19 is encoded by transcript variant 19"
                     /codon_start=1
                     /product="kinectin isoform 19"
                     /protein_id="NP_001366422.1"
                     /db_xref="GeneID:16709"
                     /db_xref="MGI:MGI:109153"
                     /translation="MELYESTYFVVLIPSVVITVIFLFFWLFMKETLYDEVLAKQKRE
                     QKLISTKTDKKKAEKKKNKKKEIQNGTLRESDSEHVPRDFKLSDASPAEDEQFVPAPL
                     NVAETSSSVRERKKKEKKQKPSLEEQVIKESDASKIPGKKVEPVLVTKQPAPPPPLEA
                     AALKKKAGQKKSKNGSEEQDKKVEMLMAPSKEQDVLLSHQDTKQEGGLGKKKGLSKKQ
                     KSENVAVLVDEPLIHATTYIPLDNANSNLMMDKREIIDMIKPDHVEGIQKSGTKKLKI
                     ETDKENAEVKFKDFLLSLKTMMFSEDEALCVVDLLKEKSGVIKEALKKSNKGELSGLL
                     HQLQEKERLLSAMKEDAAASKERCKQLTQEMMTEKERSSVVIARMKDRIGTLEKEHNI
                     FQNKMHASYQETQQMQMKFQQVQEQMEAEIAHLKQENGILRDAVSNTTNQLESKQSAE
                     LNKLRQDCGRLVSELNEKTGKLQQEGVQKKNAEQAATQLKVQLQEAERRWEEVQSYIR
                     KRTAEHEAAQQDLQSKFVAKENEVQSLHSKLTDTLVSKQQLEQRLMQLMESEQKRASK
                     EESLQIQVQDILEQNEALKAQIQQFHSQIAAQTSASVLAEELHKVIAEKDKQLKQTED
                     SLANEQDHLASKEEELKDVQNMNFLLKAEVQKLQALANEQAATAHEVEKMQKSIHVKE
                     DKIRLLEEQLQHEVASKMEELKILSEQNKALQSEVQKLQTAVSQQPNKDVVEQMEKCI
                     QEKDEKLRTVEELLETGLIQVATREEELSAIRTENSTLTREVQELKAKQSDQVSFVSL
                     IEDLKRVIHEKDGQIKSVEELLEVELLKVANKEKTVQALKQEIEVLKEEIGNAQLEKA
                     HQLSVTSQVQELQNLLRGKEEQVNSMKAALEDRDRDLRGRGTCAQVCSTPQFEELESV
                     LKEKDNEIKRIEVKLKDTESDVSKMSELLKEVQEENKFLKCQLSHQKHQQASFPSQEE
                     LQTVISEKEKEITDLCNELESLKNAVEHQRKKNNDLREKNWEAMEALASTEKMLQDRV
                     NKTSKERRQHVEAIELESKDLLKRLFPTVSVPSNLNYSEWLRGFEKKAKACVAGTSDA
                     EAVKVLEHRLKEASEMHTLLQLECEKYKSVLAETEGILQKLQRSVEQEESKWKIKADE
                     SQRMIKQMQSSFTASERELERLRQENKDMENLRREREHLEMELEKAEMERSTYVMEVR
                     ELKTQLNETHSKLQNEQTERKKVADDLHKAQQSLNSIHSKISLKAAGDTVVIENSDIS
                     PEMSEVIGKLLVRS"
     exon            690..827
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..1001
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1132
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1249
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1390
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1497
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1630
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1718
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1885
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1954
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..1992
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2083
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2152
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2190
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2272
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2341
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2376
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2467
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2536
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2537..2574
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2665
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2666..2734
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2735..2772
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2773..2863
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2864..2901
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2902..2992
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2993..3052
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3053..3087
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3178
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3179..3265
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3266..3355
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..3436
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3437..3526
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3527..3619
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3691
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3692..3775
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3776..3859
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3860..3955
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3956..4420
                     /gene="Ktn1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4392..4397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ktn1"
                     /note="hexamer: AATAAA"
     polyA_site      4420
                     /gene="Ktn1"
                     /note="major polyA site"
ORIGIN      
        1 gaagccgccc gtttcctgcg gggcggcgcg acggcacctg agctactgcg gcggggcctg
       61 ggagcgcgct gaccaggagg tagagctcgc gccgctgcat cgtcccggcc tggtacccgg
      121 ctgcgcagga ttctgcacag gctctcgtta agagaagact atggagctgt acgagtcaac
      181 gtattttgtt gtcctcatcc cttcagtagt tattaccgtc attttccttt tcttctggct
      241 tttcatgaaa gaaacattat atgatgaagt tcttgcaaaa caaaaaagag agcaaaagtt
      301 gatttctaca aaaacagata aaaagaaggc agaaaagaag aagaataaaa agaaagaaat
      361 ccagaatggg acccttcgtg aatctgattc tgagcacgtg cctcgggact ttaaattatc
      421 cgatgcttct cccgcggagg atgaacagtt tgtacctgct ccactgaatg tggcagaaac
      481 ttccagtagt gttagggaaa gaaagaagaa ggagaagaaa caaaaacctt cccttgaaga
      541 gcaggtcatc aaagaaagtg atgcatcaaa gatcccaggc aaaaaagtgg aacctgtcct
      601 agttaccaaa cagccggccc cacccccacc cctcgaagca gctgccttga agaagaaagc
      661 agggcagaag aagtctaaaa atggaagcga agagcaagat aaaaaggtgg aaatgctgat
      721 ggcaccttca aaagagcagg acgtgttgct ttcccatcaa gacactaagc aagaaggtgg
      781 actaggaaag aagaaaggct tgtcaaagaa gcaaaagtca gaaaatgttg cagtcttggt
      841 agacgagccc cttattcatg caactactta cattcctttg gataatgcta actcaaatct
      901 catgatggat aagagagaaa ttattgatat gattaaacct gatcatgttg aaggaatcca
      961 gaaatctgga acaaaaaagc tgaagattga aactgacaaa gaaaatgctg aagtgaaatt
     1021 taaagatttc cttctgtcct tgaagactat gatgttttct gaagatgaag ctctttgtgt
     1081 tgtagacctg ctgaaggaaa agtctggtgt gataaaagaa gctttaaaga agtcgaataa
     1141 aggagaactg agtggtcttc tgcatcaact tcaggagaag gagaggctgc tctctgccat
     1201 gaaggaagat gctgctgcct caaaggagcg ctgtaagcag ttgactcagg aaatgatgac
     1261 agagaaagaa agaagcagtg ttgttatagc aagaatgaaa gatcggattg gaacactaga
     1321 aaaggaacac aatatatttc aaaacaaaat gcatgccagt taccaagaga ctcagcagat
     1381 gcagatgaag tttcagcaag ttcaggaaca aatggaagca gagatagctc atttgaagca
     1441 ggaaaatggt atcctgagag atgctgtcag taacactaca aaccaactgg aaagcaagca
     1501 atccgcagag ctaaataaac tgcgccaaga ctgtgggagg ctggtgagtg aactgaacga
     1561 gaagacagga aagctgcagc aagagggagt ccagaagaag aatgcggagc aagctgctac
     1621 ccagctcaag gttcaactgc aagaagctga gaggcgctgg gaagaagttc agagctacat
     1681 caggaagagg acggcggagc atgaggctgc ccagcaagat ttacaaagta aatttgtggc
     1741 caaagaaaat gaagtccaga gtctacatag taagctcaca gacaccttgg tatcaaaaca
     1801 gcaattggag caaagactaa tgcagttgat ggaatccgag caaaagaggg cgagcaaaga
     1861 agagtctctg cagatccaag tgcaggatat tttggaacaa aacgaggctt tgaaagctca
     1921 gattcaacaa ttccattccc agatagcagc tcagacctcc gcttcagttc tagcagaaga
     1981 attacataaa gtgattgcag aaaaggacaa gcagctaaag cagactgaag attcattagc
     2041 gaatgaacag gatcacttag caagcaagga ggaggagctt aaggatgtac agaatatgaa
     2101 tttcttatta aaagctgaag tgcagaaatt gcaggccctg gctaatgagc aggctgctac
     2161 cgcacatgag gtggagaaga tgcagaagag tattcacgta aaggaagata aaataagact
     2221 gcttgaggag cagctgcagc acgaagttgc cagcaaaatg gaagaattga agattctgag
     2281 tgagcagaat aaagcattac agtcggaagt tcagaaactg cagaccgctg tttctcagca
     2341 gcctaataaa gatgttgtgg aacaaatgga aaaatgcatt caagaaaaag atgagaagtt
     2401 aaggactgtg gaagaattac tggagactgg actcattcag gtggccacca gagaggagga
     2461 gctgagtgcc ataagaacag agaactccac cctgaccaga gaagttcaag agttgaaggc
     2521 taagcagagc gatcaggttt cttttgtgtc tctaattgaa gatcttaaga gagtgatcca
     2581 tgagaaagat ggacagatca agtctgtgga agagcttcta gaagttgaac ttctcaaagt
     2641 tgcaaataag gagaaaactg tccaggcttt gaaacaggaa atagaggttc taaaagaaga
     2701 aataggaaat gcccagcttg aaaaggctca ccagctgtct gtcacttctc aagtccagga
     2761 gctgcagaac ttattaagag gcaaggaaga gcaggtgaac agcatgaagg ctgccttaga
     2821 agacagggac agagacctga gagggagagg gacgtgcgca caggtgtgtt cgacaccaca
     2881 atttgaagaa cttgaaagtg tgttgaaaga aaaggacaat gaaattaaga gaatagaagt
     2941 taaattaaag gacacggaga gtgatgtttc taagatgtca gagctattaa aggaggtaca
     3001 agaggaaaat aaattcctta agtgccagct cagtcaccag aagcaccaac aggcatcttt
     3061 tccctctcag gaagaattac aaacagtaat ttcagaaaaa gagaaagaaa taactgatct
     3121 ctgcaatgag ttagagtctt tgaagaatgc tgttgaacac cagaggaaga aaaacaatga
     3181 ccttcgggag aaaaactggg aagcaatgga agctttggca tcaactgaaa aaatgctgca
     3241 ggacagagtg aacaagactt ccaaggaaag acggcaacac gtggaagcta ttgagttaga
     3301 gtccaaagac cttctgaaga ggttatttcc aacggtgtct gtcccttcta atttgaatta
     3361 cagtgaatgg ttgcgtggct ttgaaaagaa ggcaaaagcg tgcgtagctg gaacttcaga
     3421 cgcagaggcg gtcaaggttt tggagcacag attgaaagaa gctagtgaga tgcacacttt
     3481 gctacagctg gaatgtgaaa aatacaagtc ggtccttgca gaaacagaag ggattttgca
     3541 aaaacttcag cgaagtgtgg agcaagaaga aagtaaatgg aaaattaaag ctgacgagtc
     3601 tcaaaggatg attaaacaga tgcagtcctc cttcacagcc tcagagcgag agctagagcg
     3661 actaagacaa gaaaacaaag atatggagaa tctgagaaga gaaagagagc atttggaaat
     3721 ggagctagag aaggccgaga tggagcgatc gacttacgtg atggaagtca gagagctcaa
     3781 gacacagtta aatgaaacac actcaaaact tcaaaatgaa caaacggaga gaaagaaggt
     3841 agctgatgat ttgcataagg ctcaacagtc cctgaactct atccactcaa aaatctcact
     3901 gaaagctgct ggagacactg ttgttattga gaacagtgat atttccccag aaatgagtga
     3961 agtaattggg aaattactcg ttagaagtta acagaaggcg ttgtattgta ttttgccaaa
     4021 ttaaagcctt atttatgttt taccctttct actttgtcag acactggaca gttttgtctt
     4081 ttctaatcct tgttagacta ctgacttaaa gagaaacact ttaaaaagcc aactctgtag
     4141 acaccttcag agtttagttt tataataaaa ctgtttgaat aattagacct ttacattcct
     4201 aaagatgaag taaatgtaaa cttttacctt attttgctca attaaattgt tcagaagatc
     4261 aaagtggtat agacaatgtg aaatttaaca ttttaatact gatgttgtac actgctttac
     4321 ttaacatttg ggaagaagcc gcctctgaca tcaactcaag gaaacacttt ttgttgctat
     4381 tataatcagc aaataaaggg acacttatta ttcaacatga
//
