LOCUS       NM_013571               5575 bp    mRNA    linear   ROD 13-AUG-2024
DEFINITION  Mus musculus kinase suppressor of ras 1 (Ksr1), transcript variant
            1, mRNA.
ACCESSION   NM_013571
VERSION     NM_013571.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ingersoll,M.A., Lutze,R.D., Kelmann,R.G., Kresock,D.F., Marsh,J.D.,
            Quevedo,R.V., Zuo,J. and Teitz,T.
  TITLE     KSR1 Knockout Mouse Model Demonstrates MAPK Pathway's Key Role in
            Cisplatin- and Noise-induced Hearing Loss
  JOURNAL   J Neurosci 44 (18), e2174232024 (2024)
   PUBMED   38548338
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Parvathaneni,S., Li,Z. and Sacks,D.B.
  TITLE     Calmodulin influences MAPK signaling by binding KSR1
  JOURNAL   J Biol Chem 296, 100577 (2021)
   PUBMED   33766558
  REMARK    GeneRIF: Calmodulin influences MAPK signaling by binding KSR1.
REFERENCE   3  (bases 1 to 5575)
  AUTHORS   Germino,E.A., Miller,J.P., Diehl,L., Swanson,C.J., Durinck,S.,
            Modrusan,Z., Miner,J.H. and Shaw,A.S.
  TITLE     Homozygous KSR1 deletion attenuates morbidity but does not prevent
            tumor development in a mouse model of RAS-driven pancreatic cancer
  JOURNAL   PLoS One 13 (3), e0194998 (2018)
   PUBMED   29596465
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5575)
  AUTHORS   Basu,S.K., Lee,S., Salotti,J., Basu,S., Sakchaisri,K., Xiao,Z.,
            Walia,V., Westlake,C.J., Morrison,D.K. and Johnson,P.F.
  TITLE     Oncogenic RAS-Induced Perinuclear Signaling Complexes Requiring
            KSR1 Regulate Signal Transmission to Downstream Targets
  JOURNAL   Cancer Res 78 (4), 891-908 (2018)
   PUBMED   29259016
REFERENCE   5  (bases 1 to 5575)
  AUTHORS   Inoue,S.I., Takahara,S., Yoshikawa,T., Niihori,T., Yanai,K.,
            Matsubara,Y. and Aoki,Y.
  TITLE     Activated Braf induces esophageal dilation and gastric epithelial
            hyperplasia in mice
  JOURNAL   Hum Mol Genet 26 (23), 4715-4727 (2017)
   PUBMED   28973166
REFERENCE   6  (bases 1 to 5575)
  AUTHORS   Stewart,S., Sundaram,M., Zhang,Y., Lee,J., Han,M. and Guan,K.L.
  TITLE     Kinase suppressor of Ras forms a multiprotein signaling complex and
            modulates MEK localization
  JOURNAL   Mol Cell Biol 19 (8), 5523-5534 (1999)
   PUBMED   10409742
REFERENCE   7  (bases 1 to 5575)
  AUTHORS   Yu,W., Fantl,W.J., Harrowe,G. and Williams,L.T.
  TITLE     Regulation of the MAP kinase pathway by mammalian Ksr through
            direct interaction with MEK and ERK
  JOURNAL   Curr Biol 8 (1), 56-64 (1998)
   PUBMED   9427629
REFERENCE   8  (bases 1 to 5575)
  AUTHORS   Denouel-Galy,A., Douville,E.M., Warne,P.H., Papin,C., Laugier,D.,
            Calothy,G., Downward,J. and Eychene,A.
  TITLE     Murine Ksr interacts with MEK and inhibits Ras-induced
            transformation
  JOURNAL   Curr Biol 8 (1), 46-55 (1998)
   PUBMED   9427625
REFERENCE   9  (bases 1 to 5575)
  AUTHORS   Therrien,M., Chang,H.C., Solomon,N.M., Karim,F.D., Wassarman,D.A.
            and Rubin,G.M.
  TITLE     KSR, a novel protein kinase required for RAS signal transduction
  JOURNAL   Cell 83 (6), 879-888 (1995)
   PUBMED   8521512
REFERENCE   10 (bases 1 to 5575)
  AUTHORS   Nehls,M., Luno,K., Schorpp,M., Pfeifer,D., Krause,S.,
            Matysiak-Scholze,U., Dierbach,H. and Boehm,T.
  TITLE     YAC/P1 contigs defining the location of 56 microsatellite markers
            and several genes across a 3.4-cM interval on mouse chromosome 11
  JOURNAL   Mamm Genome 6 (5), 321-331 (1995)
   PUBMED   7626882
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164833.1, U43585.1, AK137532.1 and CX204281.1.
            
            On Jan 19, 2017 this sequence version replaced NM_013571.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U43585.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               AK164833.1         2-120
            120-4200            U43585.1           8-4088
            4201-5560           AK137532.1         3900-5259
            5561-5575           CX204281.1         585-599
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 46.74 cM"
     gene            1..5575
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /note="kinase suppressor of ras 1"
                     /db_xref="GeneID:16706"
                     /db_xref="MGI:MGI:105051"
     exon            1..419
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..2816
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein Hb"
                     /codon_start=1
                     /product="kinase suppressor of Ras 1 isoform 1"
                     /protein_id="NP_038599.1"
                     /db_xref="CCDS:CCDS25117.1"
                     /db_xref="GeneID:16706"
                     /db_xref="MGI:MGI:105051"
                     /translation="MDRAALRAAAMGEKKEGGGGGAAADGGAGAAVSRALQQCGQLQK
                     LIDISIGSLRGLRTKCSVSNDLTQQEIRTLEAKLVKYICKQQQSKLSVTPSDRTAELN
                     SYPRFSDWLYIFNVRPEVVQEIPQELTLDALLEMDEAKAKEMLRRWGASTEECSRLQQ
                     ALTCLRKVTGLGGEHKMDSGWSSTDARDSSLGPPMDMLSSLGRAGASTQGPRSISVSA
                     LPASDSPVPGLSEGLSDSCIPLHTSGRLTPRALHSFITPPTTPQLRRHAKLKPPRTPP
                     PPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVTPMKFELPHGSPQLVRRDIGLSVTH
                     RFSTKSWLSQVCNVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRITFLPLARLRRTES
                     VPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNLDSSSNPSSTTSSTPSSPAPFLTS
                     SNPSSATTPPNPSPGQRDSRFSFPDISACSQAAPLSSTADSTRLDDQPKTDVLGVHEA
                     EAEEPEAGKSEAEDDEEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELG
                     EPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMG
                     ACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHK
                     DLKSKNVFYDNGKVVITDFGLFGISGVVREERRENQLKLSHDWLCYLAPEIVREMIPG
                     RDEDQLPFSKAADVYAFGTVWYELQARDWPFKHQPAEALIWQIGSGEGVRRVLASVSL
                     GKEVGEILSACWAFDLQERPSFSLLMDMLERLPKLNRRLSHPGHFWKSADINSSKVMP
                     RFERFGLGTLESGNPKM"
     exon            420..560
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            561..708
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            709..1132
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1137
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1198
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1282
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1344
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1451
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1617
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1672
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1721
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..2104
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2201
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2335
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            2336..2467
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2603
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2737
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     exon            2738..5561
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5543..5548
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /note="hexamer: AATAAA"
     polyA_site      5561
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /note="major polyA site"
ORIGIN      
        1 agtggaggct ttcgctttgc cgccgcgtct gggcgggagg aggagcggac tgggcagcag
       61 gctttcggga ctgcaggctt aggcggcgcc aaggggcgct cctagtgcag ccttcctggc
      121 tcggggcttt cctgccgagg cgcccgtgtc cccgggctcc tcgcctcggc ccccagcggc
      181 cccgatgccg aggcatggat agagcggcgt tgcgcgcggc agcgatgggc gagaaaaagg
      241 agggcggcgg cgggggcgcc gcggcggacg ggggcgcagg ggccgccgtc agccgggcgc
      301 tgcagcagtg cggccagctg cagaagctca tcgatatctc catcggcagt ctgcgcgggc
      361 tgcgcaccaa gtgctcagtg tctaacgacc tcacacagca ggagatccgg accctagagg
      421 caaagctggt gaaatacatt tgcaagcagc agcagagcaa gcttagtgtg accccaagcg
      481 acaggaccgc cgagctcaac agctacccac gcttcagtga ctggctgtac atcttcaacg
      541 tgaggcctga ggtggtgcag gagatccccc aagagctcac actggatgct ctgctggaga
      601 tggacgaggc caaagccaag gagatgctgc ggcgctgggg ggccagcacg gaggagtgca
      661 gccgcctaca gcaagccctt acctgccttc ggaaggtgac tggcctggga ggggagcaca
      721 aaatggactc aggttggagt tcaacagatg ctcgagacag tagcttgggg cctcccatgg
      781 acatgctttc ctcgctgggc agagcgggtg ccagcactca gggaccccgt tccatctccg
      841 tgtccgccct gcctgcctca gactctccgg tccccggcct cagtgagggc ctctcggact
      901 cctgtatccc cttgcacacc agcggccggc tgaccccccg ggccctgcac agcttcatca
      961 cgccccctac cacaccccag ctacgacggc acgccaagct gaagccacca aggacacccc
     1021 caccgccaag ccgcaaggtc ttccagctgc tccccagctt ccccacactc acacggagca
     1081 agtcccacga gtcccagctg ggaaaccgaa tcgacgacgt caccccgatg aagtttgaac
     1141 tccctcatgg atccccacag ctggtacgaa gggatatcgg gctctcggtg acgcacaggt
     1201 tctccacaaa gtcatggttg tcacaggtgt gcaacgtgtg ccagaagagc atgatttttg
     1261 gcgtgaagtg caaacactgc aggttaaaat gccataacaa gtgcacaaag gaagctcccg
     1321 cctgcaggat caccttcctc ccactggcca ggcttcggag gacagagtct gtcccgtcag
     1381 atatcaacaa cccagtggac agagcagcag agccccattt tggaaccctt cccaaggccc
     1441 tgacaaagaa ggagcaccct ccagccatga acctggactc cagcagcaac ccatcctcca
     1501 ccacgtcctc cacaccctca tcgccggcac ctttcctgac ctcatctaat ccctccagtg
     1561 ccaccacgcc tcccaacccg tcacctggcc agcgggacag caggttcagc ttcccagaca
     1621 tttcagcctg ttctcaggca gccccgctgt ccagcacagc cgacagtaca cggctcgacg
     1681 accagcccaa aacagatgtg ctaggtgttc acgaagcaga ggctgaggag cctgaggctg
     1741 gcaagtcaga ggcagaggat gacgaggagg atgaggtgga cgacctcccc agctcccgcc
     1801 ggccctggag gggccccatc tctcgaaagg ccagccagac cagcgtttac ctgcaagagt
     1861 gggacatccc ctttgaacag gtggaactgg gcgagcccat tggacagggt cgctggggcc
     1921 gggtgcaccg aggccgttgg catggcgagg tggccattcg gctgctggag atggacggcc
     1981 acaatcagga ccacctgaag ctgttcaaga aagaggtgat gaactaccgg cagacgcggc
     2041 atgagaacgt ggtgctcttc atgggggcct gcatgaaccc acctcacctg gccattatca
     2101 ccagcttctg caaggggcgg acattgcatt cattcgtgag ggaccccaag acgtctctgg
     2161 acatcaataa gactaggcag atcgcccagg agatcatcaa gggcatgggt tatcttcatg
     2221 caaaaggcat cgtgcacaag gacctcaagt ccaagaatgt cttctatgac aacggcaaag
     2281 tggtcatcac agacttcggg ctgtttggga tctcgggtgt ggtccgagag gaacggcgcg
     2341 agaaccaact gaaactgtca catgactggc tgtgctacct ggcccccgag atcgtacgag
     2401 aaatgatccc ggggcgggac gaggaccagc tgcccttctc caaagcagcc gatgtctatg
     2461 cattcgggac tgtgtggtat gaactacagg caagagactg gccctttaag caccagcctg
     2521 ctgaggcctt gatctggcag attggaagtg gggaaggagt acggcgcgtc ctggcatccg
     2581 tcagcctggg gaaggaagtc ggcgagatcc tgtctgcctg ctgggctttc gatctgcagg
     2641 agagacccag cttcagcctg ctgatggaca tgctggagag gctgcccaag ctgaaccggc
     2701 ggctctccca ccctgggcac ttttggaagt cggctgacat taacagcagc aaagtcatgc
     2761 cccgctttga aaggtttggc ctggggaccc tggagtccgg taatccaaag atgtagccag
     2821 ccctgcacgt tcatgcagag agtgtcttcc tttcgaaaac atgatcacga aacatgcaga
     2881 ccaccacctc aaggaatcag aagcattgca tcccaagctg cggactggga gcgtgtctcc
     2941 tccctaaagg acgtgcgtgc gtgcgtgcgt gcgtgcgtgc gtgcgtgcgt caccaaggtg
     3001 tgtggagctc aggatcgcag ccatacacgc aactccagat gataccacta ccgccagtgt
     3061 ttacacagag gtttctgcct ggcaagcttg gtattttaca gtaggtgaag atcattctgc
     3121 agaagggtgc tggcacagtg gagcagcacg gatgtcccca gcccccgttc tggaagaccc
     3181 tacagctgtg agaggcccag ggttgagcca gatgaaagaa aagctgcgtg ggtgtgggct
     3241 gtacccggaa aagggcaggt ggcaggaggt ttgccttggc ctgtgcttgg gccgagaacc
     3301 acactaagga gcagcagcct gagttaggaa tctatctgga ttacggggat cagagttcct
     3361 ggagagtgga ctcagtttct gctctgatcc aggcctgttg tgcttttttt ttttccccct
     3421 taaaaaaaaa aaagtacaga cagaatctca gcggcttcta gactgatctg atggatctta
     3481 gcccggcttc tactgcgggg gggagggggg gagggatagc cacatatctg tggagacacc
     3541 cacttcttta tctgaggcct ccaggtaggc acaaaggctg tggaactcag cctctatcat
     3601 cagacacccc cccccaatgc ctcattgacc cccttccccc agagccaagg gctagcccat
     3661 cgggtgtgtg tacagtaagt tcttggtgaa ggagaacagg gacgttggca gaagcagttt
     3721 gcagtggccc tagcatctta aaacccattg tctgtcacac cagaaggttc tagacctacc
     3781 accacttccc ttccccatct catggaaacc ttttagccca ttctgacccc tgtgtgtgct
     3841 ctgagctcag atcgggttat gagaccgccc aggcacatca gtcagggagg ctctgatgtg
     3901 agccgcagac ctctgtgttc attcctatga gctggagggg ctggactggg tggggtcaga
     3961 tgtgcttggc aggaactgtc agctgctgag cagggtggtc cctgagcgga ggataagcag
     4021 catcagactc cacaaccaga ggaagaaaga aatggggatg gagcggagac ccacgggctg
     4081 agtcccgctg tggagtggcc ttgcagctcc ctctcagtta aaactcccag taaagccaca
     4141 gttctccgag cacccaagtc tgctccagcc gtctcttaaa acaggccact ctctgagaag
     4201 ttgtggaaat ccccttcccc taggccccag cacagttatt cacactaccg gagagctggg
     4261 acagtagcat tctgacccta cctccgggtg gggaggaaga gccgtgagcc tctgttcaga
     4321 tctgcgtggg ctctccaatc atttctctac cccttcagct ttccacatta gcccttgacg
     4381 tgacctccct actccaaaac cgagttgtgt gtgccccctg cctctggcag tctcatgcct
     4441 gcaccatccc agaggccaca gtgcctgagg ctcaggccgg tctctcactg tgtaagtgtt
     4501 gtgatgttgg cctcccctca tggtagaatg tgaaccgtca ggctgccagc tgagttaacc
     4561 cttttcagtt atcccctgac accaaatcac agcaagcggc acacagccta ctgacagcag
     4621 caagcctggg tcttgctttg tgctgtgaac atcacagttg agaaagcttt gagccctgca
     4681 ttgaatatgg tcttgtgcat tggctgagaa ccagatagga atagtagtgg cccctggctg
     4741 aaggagctga ccatcttctg ggggccagtg tgctggggct gccatctgcc ccagtggagc
     4801 tatagttcta gggccctcct ccttgcttcc caagtcctgc ctctgctcgg cccttcctgt
     4861 aagagagttc ccaccgtggc ccccacttcc ttttctactt aagtgactgt gaattgaagt
     4921 taggaaagct tgcctcctgc gcactctcct cgtctcctgg gagacacatg aaggatgaca
     4981 gatactgtga attcagcccg cgtagccact atgaaggcag tgaaaaaggc ccaagcatcg
     5041 gagaacctgc ccacctaagg tgtgatgccc tttaccaggc accctgggtt cccagcctca
     5101 ccccgaggga ggccttttcc catcagagtg gcaagtagtg aatcctagct agtgctgggc
     5161 tgctctaatg catccagtca gagccctcgg ccattgtctt cctggtccag gcagggagtg
     5221 gcaaacctgt gttgctctgt agtgacaggg tggggtggga cagccacgct gtgctgcagc
     5281 gctggccagg atatcatcag tgtgtacacc tagcgtcttc tgtggccacg aggcagacat
     5341 ttatttgctt attttgaatg tgatgtaaaa tttgtacagt aaaagttttt atattttcta
     5401 tcaattgcat ttgtcttcca gaaatgcttt aatttaaatt aaaatggtta gtcttgatga
     5461 atgtgttacg ttggggtttt tgccactgct aagaatgaat gtcccctgtg gctcagccag
     5521 gcctaggggt ctggagtgtg tgaataaagt ttaccaaggt aaaaaaaaaa aaaaa
//
