LOCUS       NP_038599                873 aa            linear   ROD 28-APR-2025
DEFINITION  kinase suppressor of Ras 1 isoform 1 [Mus musculus].
ACCESSION   NP_038599
VERSION     NP_038599.1
DBSOURCE    REFSEQ: accession NM_013571.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 873)
  AUTHORS   Ingersoll,M.A., Lutze,R.D., Kelmann,R.G., Kresock,D.F., Marsh,J.D.,
            Quevedo,R.V., Zuo,J. and Teitz,T.
  TITLE     KSR1 Knockout Mouse Model Demonstrates MAPK Pathway's Key Role in
            Cisplatin- and Noise-induced Hearing Loss
  JOURNAL   J Neurosci 44 (18), e2174232024 (2024)
   PUBMED   38548338
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 873)
  AUTHORS   Parvathaneni,S., Li,Z. and Sacks,D.B.
  TITLE     Calmodulin influences MAPK signaling by binding KSR1
  JOURNAL   J Biol Chem 296, 100577 (2021)
   PUBMED   33766558
  REMARK    GeneRIF: Calmodulin influences MAPK signaling by binding KSR1.
REFERENCE   3  (residues 1 to 873)
  AUTHORS   Germino,E.A., Miller,J.P., Diehl,L., Swanson,C.J., Durinck,S.,
            Modrusan,Z., Miner,J.H. and Shaw,A.S.
  TITLE     Homozygous KSR1 deletion attenuates morbidity but does not prevent
            tumor development in a mouse model of RAS-driven pancreatic cancer
  JOURNAL   PLoS One 13 (3), e0194998 (2018)
   PUBMED   29596465
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 873)
  AUTHORS   Basu,S.K., Lee,S., Salotti,J., Basu,S., Sakchaisri,K., Xiao,Z.,
            Walia,V., Westlake,C.J., Morrison,D.K. and Johnson,P.F.
  TITLE     Oncogenic RAS-Induced Perinuclear Signaling Complexes Requiring
            KSR1 Regulate Signal Transmission to Downstream Targets
  JOURNAL   Cancer Res 78 (4), 891-908 (2018)
   PUBMED   29259016
REFERENCE   5  (residues 1 to 873)
  AUTHORS   Inoue,S.I., Takahara,S., Yoshikawa,T., Niihori,T., Yanai,K.,
            Matsubara,Y. and Aoki,Y.
  TITLE     Activated Braf induces esophageal dilation and gastric epithelial
            hyperplasia in mice
  JOURNAL   Hum Mol Genet 26 (23), 4715-4727 (2017)
   PUBMED   28973166
REFERENCE   6  (residues 1 to 873)
  AUTHORS   Stewart,S., Sundaram,M., Zhang,Y., Lee,J., Han,M. and Guan,K.L.
  TITLE     Kinase suppressor of Ras forms a multiprotein signaling complex and
            modulates MEK localization
  JOURNAL   Mol Cell Biol 19 (8), 5523-5534 (1999)
   PUBMED   10409742
REFERENCE   7  (residues 1 to 873)
  AUTHORS   Yu,W., Fantl,W.J., Harrowe,G. and Williams,L.T.
  TITLE     Regulation of the MAP kinase pathway by mammalian Ksr through
            direct interaction with MEK and ERK
  JOURNAL   Curr Biol 8 (1), 56-64 (1998)
   PUBMED   9427629
REFERENCE   8  (residues 1 to 873)
  AUTHORS   Denouel-Galy,A., Douville,E.M., Warne,P.H., Papin,C., Laugier,D.,
            Calothy,G., Downward,J. and Eychene,A.
  TITLE     Murine Ksr interacts with MEK and inhibits Ras-induced
            transformation
  JOURNAL   Curr Biol 8 (1), 46-55 (1998)
   PUBMED   9427625
REFERENCE   9  (residues 1 to 873)
  AUTHORS   Therrien,M., Chang,H.C., Solomon,N.M., Karim,F.D., Wassarman,D.A.
            and Rubin,G.M.
  TITLE     KSR, a novel protein kinase required for RAS signal transduction
  JOURNAL   Cell 83 (6), 879-888 (1995)
   PUBMED   8521512
REFERENCE   10 (residues 1 to 873)
  AUTHORS   Nehls,M., Luno,K., Schorpp,M., Pfeifer,D., Krause,S.,
            Matysiak-Scholze,U., Dierbach,H. and Boehm,T.
  TITLE     YAC/P1 contigs defining the location of 56 microsatellite markers
            and several genes across a 3.4-cM interval on mouse chromosome 11
  JOURNAL   Mamm Genome 6 (5), 321-331 (1995)
   PUBMED   7626882
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164833.1, U43585.1, AK137532.1 and CX204281.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U43585.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 46.74 cM"
     Protein         1..873
                     /product="kinase suppressor of Ras 1 isoform 1"
                     /EC_number="2.7.11.1"
                     /note="protein Hb"
                     /calculated_mol_wt=96624
     Region          1..170
                     /region_name="Mediates association with membranes.
                     /evidence=ECO:0000269|PubMed:23250398"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61097.1)"
     Region          1..24
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61097.1)"
     Region          39..92
                     /region_name="SAM_KSR1_N"
                     /note="Kinase suppressor RAS 1 N-terminal helical hairpin;
                     pfam20406"
                     /db_xref="CDD:466555"
     Region          100..166
                     /region_name="SAM_KSR1"
                     /note="SAM like domain present in kinase suppressor RAS 1;
                     pfam13543"
                     /db_xref="CDD:463916"
     Region          174..230
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61097.1)"
     Region          251..281
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61097.1)"
     Site            256
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q8IVT5; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Site            260
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q8IVT5; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Site            297
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by MARK3.
                     /evidence=ECO:0000269|PubMed:11741534,
                     ECO:0000269|PubMed:12941695; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Site            320
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Region          332..378
                     /region_name="C1_KSR1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in kinase suppressor of Ras 1 (KSR1) and similar
                     proteins; cd20872"
                     /db_xref="CDD:410422"
     Site            337
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8IVT5; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Site            392
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by MARK3.
                     /evidence=ECO:0000269|PubMed:12941695; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Site            411
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q8IVT5; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Region          416..473
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61097.1)"
     Region          506..544
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61097.1)"
     Site            518
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     Region          562..840
                     /region_name="STKc_KSR1"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Kinase Suppressor of Ras 1; cd14152"
                     /db_xref="CDD:271054"
     Site            order(569..573,577,587,589,620,636..639,643,645,683,685,
                     687..688,690,700,703,724..727)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:271054"
     Site            order(569,571..573,577,587,589,636..639,685,687..688,690,
                     699..700)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271054"
     Site            order(573,643,645,683,685,687,703,724..727)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:271054"
     Site            699..727
                     /site_type="other"
                     /note="putative activation loop (A-loop)"
                     /db_xref="CDD:271054"
     Site            order(719,721..723,735..736,738,774..777,780..782,
                     784..785)
                     /site_type="other"
                     /note="MEK interface [polypeptide binding]"
                     /db_xref="CDD:271054"
     Site            838
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:21102438; propagated from
                     UniProtKB/Swiss-Prot (Q61097.1)"
     CDS             1..873
                     /gene="Ksr1"
                     /gene_synonym="B-KSR1; D11Bhm183e; D11Bhm184e; hb; Ksr;
                     mKSR1; RSU2"
                     /coded_by="NM_013571.3:195..2816"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS25117.1"
                     /db_xref="GeneID:16706"
                     /db_xref="MGI:MGI:105051"
ORIGIN      
        1 mdraalraaa mgekkegggg gaaadggaga avsralqqcg qlqklidisi gslrglrtkc
       61 svsndltqqe irtleaklvk yickqqqskl svtpsdrtae lnsyprfsdw lyifnvrpev
      121 vqeipqeltl dallemdeak akemlrrwga steecsrlqq altclrkvtg lggehkmdsg
      181 wsstdardss lgppmdmlss lgragastqg prsisvsalp asdspvpgls eglsdscipl
      241 htsgrltpra lhsfitpptt pqlrrhaklk pprtppppsr kvfqllpsfp tltrskshes
      301 qlgnriddvt pmkfelphgs pqlvrrdigl svthrfstks wlsqvcnvcq ksmifgvkck
      361 hcrlkchnkc tkeapacrit flplarlrrt esvpsdinnp vdraaephfg tlpkaltkke
      421 hppamnldss snpssttsst psspapflts snpssattpp npspgqrdsr fsfpdisacs
      481 qaaplsstad strlddqpkt dvlgvheaea eepeagksea eddeedevdd lpssrrpwrg
      541 pisrkasqts vylqewdipf eqvelgepig qgrwgrvhrg rwhgevairl lemdghnqdh
      601 lklfkkevmn yrqtrhenvv lfmgacmnpp hlaiitsfck grtlhsfvrd pktsldinkt
      661 rqiaqeiikg mgylhakgiv hkdlksknvf ydngkvvitd fglfgisgvv reerrenqlk
      721 lshdwlcyla peivremipg rdedqlpfsk aadvyafgtv wyelqardwp fkhqpaeali
      781 wqigsgegvr rvlasvslgk evgeilsacw afdlqerpsf sllmdmlerl pklnrrlshp
      841 ghfwksadin sskvmprfer fglgtlesgn pkm
//
