LOCUS       NM_025327                540 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Mus musculus keratinocyte associated protein 2 (Krtcap2),
            transcript variant 1, mRNA.
ACCESSION   NM_025327
VERSION     NM_025327.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 540)
  AUTHORS   Choquet,H., Melles,R.B., Anand,D., Yin,J., Cuellar-Partida,G.,
            Wang,W., Hoffmann,T.J., Nair,K.S., Hysi,P.G., Lachke,S.A. and
            Jorgenson,E.
  CONSRTM   23andMe Research Team
  TITLE     A large multiethnic GWAS meta-analysis of cataract identifies new
            risk loci and sex-specific effects
  JOURNAL   Nat Commun 12 (1), 3595 (2021)
   PUBMED   34127677
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 540)
  AUTHORS   Wilson,C.M., Magnaudeix,A., Yardin,C. and Terro,F.
  TITLE     DC2 and keratinocyte-associated protein 2 (KCP2), subunits of the
            oligosaccharyltransferase complex, are regulators of the
            gamma-secretase-directed processing of amyloid precursor protein
            (APP)
  JOURNAL   J Biol Chem 286 (36), 31080-31091 (2011)
   PUBMED   21768116
  REMARK    GeneRIF: components of the oligosaccharyltransferase complex DC2
            and KCP2 can interact with the gamma-secretase and affect the
            amyloid precursor protein processing pathway.
REFERENCE   4  (bases 1 to 540)
  AUTHORS   Shibatani,T., David,L.L., McCormack,A.L., Frueh,K. and Skach,W.R.
  TITLE     Proteomic analysis of mammalian oligosaccharyltransferase reveals
            multiple subcomplexes that contain Sec61, TRAP, and two potential
            new subunits
  JOURNAL   Biochemistry 44 (16), 5982-5992 (2005)
   PUBMED   15835887
REFERENCE   5  (bases 1 to 540)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK002479.1 and AW495353.1.
            
            On Jul 16, 2015 this sequence version replaced NM_025327.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP776925.1, BC043030.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-516               AK002479.1         2-517
            517-540             AW495353.1         1-24                c
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 39.03 cM"
     gene            1..540
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /note="keratinocyte associated protein 2"
                     /db_xref="GeneID:66059"
                     /db_xref="MGI:MGI:1913309"
     exon            1..78
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..485
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     oligosaccharyl transferase subunit KCP2;
                     dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit KCP2"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit KCP2 isoform 1"
                     /protein_id="NP_079603.1"
                     /db_xref="CCDS:CCDS50960.1"
                     /db_xref="GeneID:66059"
                     /db_xref="MGI:MGI:1913309"
                     /translation="MVVGTGTSLALSSLLSLLLFAGMQIYSRQLASTEWLTIQGGLLG
                     SGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSM
                     VGLYYINKISSTLYQATAPVLTPAKITGKGKKRN"
     exon            79..233
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /inference="alignment:Splign:2.1.0"
     exon            234..297
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /inference="alignment:Splign:2.1.0"
     exon            298..364
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /inference="alignment:Splign:2.1.0"
     exon            365..523
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      498..503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /note="hexamer: AATAAA"
     polyA_site      517
                     /gene="Krtcap2"
                     /gene_synonym="0610010I12Rik; Kcp2"
                     /note="major polyA site"
ORIGIN      
        1 gcgtacagac aaccgcgccg gctttggctc ccctctttct ccagggacac ggattggacc
       61 acggacgggg cgtgatggta gtcggtaccg ggacctcctt ggcgctgtcc tccctcctgt
      121 ccctgctgct cttcgctggc atgcagatct acagccgcca gctggcctcc accgagtggc
      181 tcaccattca ggggggcctg ctgggctccg gccttttcgt cttctccctg actgccttca
      241 ataatctgga gaatctggtc tttggtaaag gcttccaagc aaaaatcttc cctgagattc
      301 tcctctgctt gctgttggcg ctctttgcct ccggcctcat ccaccgagtt tgtgtcacca
      361 cttgcttcat cttctccatg gttggtctgt actacatcaa caagatatcg tccactctgt
      421 accaggcaac agctccagtt ctcacaccgg ccaaaatcac tggcaagggc aagaagagaa
      481 actaactgtg aatgagtaat aaagttgtgt ctttgtagct gtgaaaaaaa aaaaaaaaaa
//
