LOCUS       NM_008470               1580 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Mus musculus keratin 16 (Krt16), transcript variant 2, mRNA.
ACCESSION   NM_008470
VERSION     NM_008470.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Steen,K., Chen,D., Wang,F., Majumdar,R., Chen,S., Kumar,S.,
            Lombard,D.B., Weigert,R., Zieman,A.G., Parent,C.A. and
            Coulombe,P.A.
  TITLE     A role for keratins in supporting mitochondrial organization and
            function in skin keratinocytes
  JOURNAL   Mol Biol Cell 31 (11), 1103-1111 (2020)
   PUBMED   32213122
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Zieman,A.G., Poll,B.G., Ma,J. and Coulombe,P.A.
  TITLE     Altered keratinocyte differentiation is an early driver of keratin
            mutation-based palmoplantar keratoderma
  JOURNAL   Hum Mol Genet 28 (13), 2255-2270 (2019)
   PUBMED   31220272
REFERENCE   3  (bases 1 to 1580)
  AUTHORS   Kerns,M.L., Hakim,J.M.C., Zieman,A., Lu,R.G. and Coulombe,P.A.
  TITLE     Sexual Dimorphism in Response to an NRF2 Inducer in a Model for
            Pachyonychia Congenita
  JOURNAL   J Invest Dermatol 138 (5), 1094-1100 (2018)
   PUBMED   29277538
REFERENCE   4  (bases 1 to 1580)
  AUTHORS   Inoue,S.I., Takahara,S., Yoshikawa,T., Niihori,T., Yanai,K.,
            Matsubara,Y. and Aoki,Y.
  TITLE     Activated Braf induces esophageal dilation and gastric epithelial
            hyperplasia in mice
  JOURNAL   Hum Mol Genet 26 (23), 4715-4727 (2017)
   PUBMED   28973166
REFERENCE   5  (bases 1 to 1580)
  AUTHORS   Maruthappu,T., Chikh,A., Fell,B., Delaney,P.J., Brooke,M.A.,
            Levet,C., Moncada-Pazos,A., Ishida-Yamamoto,A., Blaydon,D.,
            Waseem,A., Leigh,I.M., Freeman,M. and Kelsell,D.P.
  TITLE     Rhomboid family member 2 regulates cytoskeletal stress-associated
            Keratin 16
  JOURNAL   Nat Commun 8, 14174 (2017)
   PUBMED   28128203
  REMARK    GeneRIF: Study identified iRHOM2 as a novel regulator of K16 in
            humans and mice, with important implications for palmoplantar
            keratodermas, wound healing, inflammatory skin disease and cancers.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1580)
  AUTHORS   Porter,R.M., Hutcheson,A.M., Rugg,E.L., Quinlan,R.A. and Lane,E.B.
  TITLE     cDNA cloning, expression, and assembly characteristics of mouse
            keratin 16
  JOURNAL   J Biol Chem 273 (48), 32265-32272 (1998)
   PUBMED   9822705
REFERENCE   7  (bases 1 to 1580)
  AUTHORS   Heyden,A., Lutzow-Holm,C., Clausen,O.P., Brandtzaeg,P. and
            Huitfeldt,H.S.
  TITLE     Expression of keratins K6 and K16 in regenerating mouse epidermis
            is less restricted by cell replication than the expression of K1
            and K10
  JOURNAL   Epithelial Cell Biol 3 (3), 96-101 (1994)
   PUBMED   7534576
REFERENCE   8  (bases 1 to 1580)
  AUTHORS   Jiang,C.K., Magnaldo,T., Ohtsuki,M., Freedberg,I.M., Bernerd,F. and
            Blumenberg,M.
  TITLE     Epidermal growth factor and transforming growth factor alpha
            specifically induce the activation- and
            hyperproliferation-associated keratins 6 and 16
  JOURNAL   Proc Natl Acad Sci U S A 90 (14), 6786-6790 (1993)
   PUBMED   7688128
REFERENCE   9  (bases 1 to 1580)
  AUTHORS   Stoler,A., Kopan,R., Duvic,M. and Fuchs,E.
  TITLE     Use of monospecific antisera and cRNA probes to localize the major
            changes in keratin expression during normal and abnormal epidermal
            differentiation
  JOURNAL   J Cell Biol 107 (2), 427-446 (1988)
   PUBMED   2458356
REFERENCE   10 (bases 1 to 1580)
  AUTHORS   Steinert,P.M., Rice,R.H., Roop,D.R., Trus,B.L. and Steven,A.C.
  TITLE     Complete amino acid sequence of a mouse epidermal keratin subunit
            and implications for the structure of intermediate filaments
  JOURNAL   Nature 302 (5911), 794-800 (1983)
   PUBMED   6188955
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF053235.1.
            
            [WARNING] On May 7, 2024 this sequence was replaced by NM_008470.2.
            
            Summary: The protein encoded by this gene is a member of the
            keratin gene family. The keratins are intermediate filament
            proteins responsible for the structural integrity of epithelial
            cells and are subdivided into cytokeratins and hair keratins. The
            encoded protein is a cytokeratin and acts as an innate immune
            system effector, promoting the inflammatory response upon breach of
            the skin barrier. Defects in this gene are a cause of pachyonychia
            congenita. Two transcript variants encoding different isoforms have
            been found for this gene. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is 1 aa shorter compared to
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF053235.1, BC103666.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 63.44 cM"
     gene            1..1580
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /note="keratin 16"
                     /db_xref="GeneID:16666"
                     /db_xref="MGI:MGI:96690"
     exon            1..531
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..1422
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /note="isoform 2 is encoded by transcript variant 2;
                     keratin, type I cytoskeletal 16; keratin intermediate
                     filament 16a; keratin intermediate filament 16b;
                     cytokeratin-16; keratin complex 1, acidic, gene 16"
                     /codon_start=1
                     /product="keratin, type I cytoskeletal 16 isoform 2"
                     /protein_id="NP_032496.1"
                     /db_xref="CCDS:CCDS25414.1"
                     /db_xref="GeneID:16666"
                     /db_xref="MGI:MGI:96690"
                     /translation="MATCSRQFTSSSSMKGSCGIGGGSSRMSSILAGGSCRAPSTCGG
                     MSVTSSRFSSGGVCGIGGGYGGSFSSSSFGGGLGSGFGGRFDGFGGGFGAGLGGGLGG
                     GIGDGLLVGSEKVTMQNLNDRLATYLDKVRALEEANRDLEVKIRDWYQRQRPTEIKDY
                     SPYFKTIEDLKSKIIIATQENAQFTLQIDNARLAADDFRTKYENELFLRQSVEGDING
                     LRKVLDELTLSRADLEMQIENLREELAFLKKNHEEEMLALRGQTGGDVNVEMDAAPGV
                     DLSRILNEMRDQYEQMAEKNRRDVEAWFLRKTEELNKEVASNSDLIQSNRSEVAELRR
                     VFQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLISSVEEQLAQLRCEMEQQ
                     SQEYNILLDVKTRLEQEIATYRRLLDGENIHSSSQHSSGQSYSSREVFSSSSRQPRSI
                     LKEQGSTSFSQSQSQSSRD"
     exon            532..614
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     exon            615..771
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     exon            772..933
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1059
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1280
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1327
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1565
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1541..1546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
     polyA_site      1565
                     /gene="Krt16"
                     /gene_synonym="CK-16; K16; Krt1-16"
ORIGIN      
        1 cagttcctca gcatggccac ctgcagccgc cagttcacct cctccagctc catgaagggc
       61 tcctgtggca tcggtggtgg ctctagccgc atgtcctcca tcctggctgg aggatcctgc
      121 cgggctccca gcacctgcgg gggcatgtca gttacctcct ctcgcttctc ctctggggga
      181 gtctgtggga ttggaggtgg ctatggtggg agcttcagca gcagcagttt tggtggagga
      241 cttggtagtg gatttggtgg tcgatttgat ggatttggtg gtggttttgg tgctggtctt
      301 ggtggtggtc ttggcggtgg tattggtgat gggctcctgg tgggcagtga gaaagtgacc
      361 atgcagaacc tcaatgaccg cctggccacc tacctggaca aggtgcgtgc cctggaagag
      421 gccaacagag acctggaggt gaagatccgg gactggtacc agaggcagcg gcccactgag
      481 atcaaagact acagccccta cttcaagacc attgaggacc tgaagagcaa gatcattatt
      541 gccacccagg agaatgcaca gttcactttg cagattgaca atgccaggct ggcagctgat
      601 gacttcagga ccaagtacga gaatgagctg ttcttgcggc agtccgtgga gggtgacatc
      661 aatggcctgc gcaaggtgct agatgagctg accctgtcca gagctgacct ggaaatgcag
      721 attgaaaacc tcagagaaga gctggccttc ctgaagaaga accatgagga ggagatgctt
      781 gccttgaggg gtcagactgg tggggacgtc aatgtggaga tggacgcagc ccccggtgtg
      841 gacctcagcc gcattctgaa tgagatgagg gaccagtatg agcagatggc agagaagaac
      901 cgcagagatg tggaggcctg gttcctgaga aagactgagg agctgaacaa agaggtggcc
      961 tctaacagtg atctaatcca gagcaaccgc agcgaggtgg ctgagctccg cagggtgttc
     1021 cagggcctgg agattgaact gcagtcccag ctcagcatga aagcatcctt ggagaacagc
     1081 ctagaagaga ccaaaggcag atactgtatg cagctgtccc agatccaggg tttgatcagc
     1141 agtgtggagg agcagctggc tcagcttcgc tgcgagatgg agcagcagag ccaggagtac
     1201 aacatcttgt tggatgtgaa gacaaggctg gagcaggaga tcgccaccta ccgccgtctg
     1261 ctggatggcg agaatatcca ctcctcctca cagcactcct ctggacagtc ctattcttct
     1321 cgagaagtct tctcctcatc ctcccgccag ccccggtcca tcctcaagga gcaaggttca
     1381 accagcttca gccagagcca aagtcagagt tccagggact aatgttttgc ctagagcctc
     1441 ctcacccaca actgcctctc aagctgaggg cttggggcag gaccctgttt tctttgcgca
     1501 ttccccatct gtctccccta ccctctcatg gtggtaggct aataaagctt tttggttgat
     1561 gcaaaaaaaa aaaaaaaaaa
//
