LOCUS       NM_029617              12901 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus kinetochore scaffold 1 (Knl1), transcript variant 1,
            mRNA.
ACCESSION   NM_029617 NM_001081042 XM_130428
VERSION     NM_029617.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 12901)
  AUTHORS   Zhao,Y., Yang,J., Lu,D., Zhu,Y., Liao,K., Tian,Y. and Yin,R.
  TITLE     The Loss-Function of KNL1 Causes Oligospermia and Asthenospermia in
            Mice by Affecting the Assembly and Separation of the Spindle
            through Flow Cytometry and Immunofluorescence
  JOURNAL   Sensors (Basel) 23 (5), 2571 (2023)
   PUBMED   36904774
  REMARK    GeneRIF: The Loss-Function of KNL1 Causes Oligospermia and
            Asthenospermia in Mice by Affecting the Assembly and Separation of
            the Spindle through Flow Cytometry and Immunofluorescence.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12901)
  AUTHORS   Mora-Bermudez,F., Kanis,P., Macak,D., Peters,J., Naumann,R.,
            Xing,L., Sarov,M., Winkler,S., Oegema,C.E., Haffner,C.,
            Wimberger,P., Riesenberg,S., Maricic,T., Huttner,W.B. and Paabo,S.
  TITLE     Longer metaphase and fewer chromosome segregation errors in modern
            human than Neanderthal brain development
  JOURNAL   Sci Adv 8 (30), eabn7702 (2022)
   PUBMED   35905187
REFERENCE   4  (bases 1 to 12901)
  AUTHORS   Yue,W., Wang,Y., Meng,T.G., Zhang,H.Y., Zhang,X.R., Ouyang,Y.C.,
            Hou,Y., Schatten,H., Wang,Z.B. and Sun,Q.Y.
  TITLE     Kinetochore scaffold 1 regulates SAC function during mouse oocyte
            meiotic maturation
  JOURNAL   FASEB J 36 (3), e22210 (2022)
   PUBMED   35167144
  REMARK    GeneRIF: Kinetochore scaffold 1 regulates SAC function during mouse
            oocyte meiotic maturation.
REFERENCE   5  (bases 1 to 12901)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 12901)
  AUTHORS   Shi,L., Qalieh,A., Lam,M.M., Keil,J.M. and Kwan,K.Y.
  TITLE     Robust elimination of genome-damaged cells safeguards against brain
            somatic aneuploidy following Knl1 deletion
  JOURNAL   Nat Commun 10 (1), 2588 (2019)
   PUBMED   31197172
  REMARK    GeneRIF: Study in mouse model finds that following Knl1 deletion,
            segregation errors in mitotic neural progenitor cells give rise to
            DNA damage on the missegregated chromosomes. This triggers rapid
            p53 activation and robust apoptotic and microglial phagocytic
            responses that extensively eliminate cells with somatic genome
            damage, thus causing microcephaly.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 12901)
  AUTHORS   Varma,D. and Salmon,E.D.
  TITLE     The KMN protein network--chief conductors of the kinetochore
            orchestra
  JOURNAL   J Cell Sci 125 (Pt 24), 5927-5936 (2012)
   PUBMED   23418356
  REMARK    Review article
REFERENCE   8  (bases 1 to 12901)
  AUTHORS   Krenn,V., Wehenkel,A., Li,X., Santaguida,S. and Musacchio,A.
  TITLE     Structural analysis reveals features of the spindle checkpoint
            kinase Bub1-kinetochore subunit Knl1 interaction
  JOURNAL   J Cell Biol 196 (4), 451-467 (2012)
   PUBMED   22331848
REFERENCE   9  (bases 1 to 12901)
  AUTHORS   Lampert,F. and Westermann,S.
  TITLE     A blueprint for kinetochores - new insights into the molecular
            mechanics of cell division
  JOURNAL   Nat Rev Mol Cell Biol 12 (7), 407-412 (2011)
   PUBMED   21633384
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772264.8.
            
            On Apr 26, 2018 this sequence version replaced NM_029617.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219380.68430.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               AL772264.8         164968-165072       c
            106-156             AL772264.8         154860-154910       c
            157-196             AL772264.8         153797-153836       c
            197-256             AL772264.8         152588-152647       c
            257-318             AL772264.8         149770-149831       c
            319-371             AL772264.8         148094-148146       c
            372-424             AL772264.8         146190-146242       c
            425-4921            AL772264.8         139572-144068       c
            4922-5060           AL772264.8         135821-135959       c
            5061-5128           AL772264.8         135188-135255       c
            5129-5227           AL772264.8         134195-134293       c
            5228-5343           AL772264.8         130566-130681       c
            5344-5434           AL772264.8         125541-125631       c
            5435-5551           AL772264.8         124599-124715       c
            5552-5639           AL772264.8         123305-123392       c
            5640-5717           AL772264.8         118108-118185       c
            5718-5757           AL772264.8         117886-117925       c
            5758-5859           AL772264.8         116962-117063       c
            5860-5960           AL772264.8         114673-114773       c
            5961-6029           AL772264.8         112361-112429       c
            6030-6139           AL772264.8         111369-111478       c
            6140-6258           AL772264.8         110091-110209       c
            6259-6381           AL772264.8         109570-109692       c
            6382-12901          AL772264.8         101694-108213       c
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.85 cM"
     gene            1..12901
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /note="kinetochore scaffold 1"
                     /db_xref="GeneID:76464"
                     /db_xref="MGI:MGI:1923714"
     exon            1..105
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            106..156
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..6481
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein CASC5; kinetochore-null protein 1; cancer
                     susceptibility candidate 5; outer kinetochore KNL1 complex
                     subunit KNL1"
                     /codon_start=1
                     /product="outer kinetochore KNL1 complex subunit KNL1
                     isoform 1"
                     /protein_id="NP_083893.2"
                     /db_xref="CCDS:CCDS38203.1"
                     /db_xref="GeneID:76464"
                     /db_xref="MGI:MGI:1923714"
                     /translation="MDGVYSEANEENDNTQRPVRRQHSSILKPPRSPLQDLKCGNQTN
                     QEPNPPRKRKSSRRVSFADTIKVFQTESHMKTERNSEISGMNTLLCAPIQTQMQQKEF
                     SITDCNHERKHANDQTVIFSDENQMDLTASHTVMITKGLSDCTKNENSTKIDTTSFLE
                     NLKHHAANSRIKKDLACSTVSLSQNIFSEKINSDNFIKRLKTGKHISSSTELDKENAE
                     IPVYSKDSNSASSTYQMHASLGVDENSSNRTRIFREQDDGMNLTQCHTACIKTWIPPS
                     TEAKIGEFKGDKTIYGNECMELTTNYTIQVLSSENNLSERETQTQNGMNVTTVDGATA
                     PAPEKKTALKDKLNAAFQGSFPNPENKIHIIKCHPIESETHTVTQISSQSASTLAVTS
                     KSICSSPAIEGYKTIFHSSSNDAMELTKCLSAMEEEKKLLKADDKYSKICTNPDAGPL
                     REKTIYLEEDSMDITKSHTVAIDNKIFKHDQENIKKEIIAIPIFEKEMVLRNLMPMSK
                     DEKRDVNYISVPQVSKESLQRSQTNTLSVSLTDKKMEFLADEDMDLTKSHTTKLSQVI
                     PTTFDLASKNVTKSYSHSKSPLNEWESLDKQVVLGQHSKLPLPQRKDRDDPDCSHHKI
                     MYSEELQTMDLTKSHTIVIGFGPSEVQEHSKINLEHKNSQLTAESIQTAVNVPAANSR
                     VVTTNDMDMLKDRSTHKPELLKEKQNIKIYGRKSIGRLKIDKTILFSEGNEGDMDITK
                     SCTVKINHRSLLDKHDSHLVSLAGTSKTILHARGQVEMEINRSHTTPLECKIISPSDI
                     TPGDLDKTMMSIDDHEELDMTKSHTVFIDYQAEAKGVLPDRLDFQLSKKESLQKPKVT
                     SLAEEIYISKNSESNHLPAKGSQLTILEEGSNSGLGEETNDAQKPGFLNELLSGKTQR
                     RKSLSLKNKSITFPENDKSYREIPQSSAVEINNETRLEDRKGFSFVPLAGTSKPVLSA
                     YGPEDMEISIGQTTASEYKTVPPEEITTIPMDKTVMFVDNFGDLDVTRSHTVFIDCQA
                     KEKVLDEYTNLGIQKTKTLSGSEGDTHIQEITKNPAAQHKHHMTTVIPSSTVVSDQSS
                     MKIKFHKADRDEEVKGKEVEANMLKQTKPESCLLNITDGKNVDFTSSYTADVCRSSDK
                     YSSLPNISSSDNSGGNTMSLCDKNKEKAYNCQVPNEFTYAAILPSTYHMDSKKLSVFP
                     PCPSKEVTQTESAIALLKDEDPVEEPLGEMATFNSKHVSLNLAKDQTEAFVDVSVASQ
                     PHLSAQQSPSTQKGQDVARRDEGILAKAGKKALPFLLENVAASTWENESKIPTNVEHF
                     AVTYEKELSISIQTDKCNTNVQSPSNSALTTQVIQTHANAEGALDFLVPSTVSCFSST
                     KPSLSNLNRKTEEVLDFQTVNLLPPAEQLLEEGSQAHSMSIVQATEIYRLGSRNDRDE
                     ESKTFCNEAETTSVPLKTAVKDKTRRCSLGIFLPKLPSKRSCSITGVDDLEQILADAA
                     DLTQLETQPVCSKDPGIGSVAAKLNLSPSQFINEENLPVYPGEILSSDSVSLDIEESV
                     LIDTSQRESLPSENKTENCRAQKRTRVEENDVTNEKKIRTHDSAQDQEIFDNHMEEDI
                     NKNVNSVLLKSLSRTPSSCSSSLDSIKSDGLSLDVSTQRNSQMESQFLGDTISEESLK
                     EKLKDGQITIKEFFILLQVHILIQKPRQSTLPAKFTINTLPTTEDLMLRQYVYGPRIQ
                     IYKEDCELLRQKIDELKISALNQNKLLADVNRNLWEKVKDYSDEELKNYGIYLNKIKS
                     RYTKMTKVFNHQGKVALYNKLVHSAENEKNKLQIKINEMDTILKKINNCLAEVETETK
                     NLENEEKNDAMEEWDSEMRDAEKELEQLKTEEEELQRKFLEVETQKTQTLAQIEFIKE
                     QTTKTEELLDQLSLSEWDVIEWSDDQAVFTFVYDSIELIITFGEPLVGLPFLDKACRK
                     INALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVN
                     HCRLLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVP
                     LPFTIHNHIGKTGHDEIAAIISKVPLEENYLKNVVKQIYQDLLKD"
     exon            157..196
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            197..256
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            257..318
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            319..371
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            372..424
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            425..4921
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            4922..5060
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5061..5128
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5129..5227
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5228..5343
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5344..5434
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5435..5551
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5552..5639
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5640..5717
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5718..5757
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5758..5859
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5860..5960
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            5961..6029
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            6030..6139
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            6140..6258
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            6259..6381
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     exon            6382..12901
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8864..8869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /note="hexamer: ATTAAA"
     polyA_site      8888
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
     regulatory      12001..12006
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /note="hexamer: AATAAA"
     polyA_site      12020
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /note="major polyA site"
     regulatory      12875..12880
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
                     /note="hexamer: AATAAA"
     polyA_site      12901
                     /gene="Knl1"
                     /gene_synonym="2310043D08Rik; 5730505K17Rik; Casc5"
ORIGIN      
        1 actgagcatg cgcatcacta gggcggttag atttgaatac ttcagggacg ttgagaggtg
       61 acgtccgact ccaaggagcg cgaggacccc agacaagtca agcagaaaaa agttttcaaa
      121 aatggatggg gtatattcag aagctaatga agagaatgac aatacacaga gacctgttag
      181 aagacagcat tcctcaattt tgaaaccccc aaggagtcct cttcaggacc tcaaatgtgg
      241 aaatcaaaca aaccaggaac ctaatcctcc aagaaaaagg aaaagctctc gtcgagtcag
      301 ctttgcagat actataaaag tattccagac agagtctcac atgaaaacag agagaaattc
      361 agaaatttca ggtatgaaca ctttgctttg tgctccaatt cagactcaga tgcaacaaaa
      421 ggagttctca attacagact gcaaccatga aaggaaacat gcaaatgatc agacagtcat
      481 cttttcagat gaaaaccaga tggatctgac agcaagtcac actgtaatga ttaccaaagg
      541 tctttcagac tgtaccaaaa atgaaaattc tacaaaaata gataccacgt cctttctgga
      601 gaatttaaag catcatgctg caaattcaag aattaaaaag gatttagcat gttccacagt
      661 ttccttaagt caaaacattt tctcagaaaa gatcaattct gacaacttca taaaaagatt
      721 gaagacagga aaacatattt cgtcttctac agagcttgat aaggaaaatg ctgagatacc
      781 tgtttattcc aaagactcaa acagtgcctc ttcaacatat caaatgcatg catctcttgg
      841 tgtagatgaa aatagtagta ataggactag aatctttaga gaacaagatg atggtatgaa
      901 tttgacccaa tgtcatacag cctgtattaa gacttggatt cctccatcca cggaggccaa
      961 aataggagaa ttcaaaggtg ataaaacaat ttatggcaat gagtgtatgg agttgacaac
     1021 taactatacg atacaggttt tgtcatcaga aaataattta tctgaaagag aaactcaaac
     1081 tcaaaatggt atgaatgtta caacagttga tggagctaca gctccagctc cagagaagaa
     1141 aacagccctt aaggataaac taaatgctgc ttttcaaggc tctttcccaa accctgaaaa
     1201 taaaattcat attatcaaat gtcatcctat agagtcagaa actcatacag tcacacagat
     1261 ttctagtcaa agtgccagca cattggctgt aacttcaaaa tctatatgtt ctagtccagc
     1321 tattgagggg tataagacaa tttttcattc tagtagcaat gatgcaatgg aactgactaa
     1381 gtgtctatca gctatggaag aggaaaaaaa attgctgaag gctgatgata agtattctaa
     1441 aatatgtacc aatccagatg ctggcccact cagagagaaa actatttatt tagaagagga
     1501 tagcatggac attaccaaaa gtcacaccgt tgcaatagat aataaaatat ttaaacatga
     1561 tcaagaaaac attaaaaaag aaataatagc cataccaata tttgaaaagg aaatggtgct
     1621 ccgaaatctt atgcctatgt caaaagatga gaaaagggat gtaaattata tttcagttcc
     1681 tcaagtatct aaggaaagct tacaacggag tcagacaaac actttatctg tttcattgac
     1741 tgacaaaaag atggaattct tggcagatga agatatggat ctgactaaaa gtcacacaac
     1801 taagttaagt caagttattc ctacaacttt tgacttagca tcaaagaatg tcaccaaatc
     1861 atactctcac agcaaaagcc ctttaaatga gtgggaaagc ctggataaac aagtagtgct
     1921 aggccagcat tctaaacttc ctttaccaca gaggaaagat agagatgatc ctgactgttc
     1981 tcatcataag ataatgtatt cagaggagtt acaaactatg gatctaacca agagccacac
     2041 tattgtaatt ggctttggtc cttctgaagt acaagaacat agtaaaatta atttagaaca
     2101 caaaaacagc caacttacag cagaaagcat acaaacagct gtgaacgttc ctgcagcaaa
     2161 ctctagagta gtaacaacta atgatatgga catgttaaag gacagaagta cacataagcc
     2221 agaattgctg aaggaaaagc aaaacatcaa aatctatgga agaaaaagta ttggtagact
     2281 gaaaattgat aaaactatat tattttcaga gggtaatgag ggtgatatgg atatcaccaa
     2341 gagttgcaca gtcaaaataa atcatagatc tttattagat aagcatgatt ctcatttagt
     2401 atctttggca ggaacttcta aaaccatttt gcatgcacgt ggtcaggttg aaatggaaat
     2461 caatagaagt cacacaactc ctttagaatg taaaattatc tcgcccagtg atataactcc
     2521 tggtgatctg gataagacta tgatgtctat agatgatcat gaggaactag acatgaccaa
     2581 gtcccatact gttttcattg actaccaagc agaggcaaaa ggtgtacttc cagacagact
     2641 tgactttcaa ttatccaaaa aggaaagcct acagaagccg aaagtgacat ctctggctga
     2701 agagatttac atttcaaaaa atagtgaaag caaccatcta ccagcaaaag gcagccagct
     2761 aacaatatta gaggaagggt ctaatagcgg gcttggagag gaaactaatg atgcacaaaa
     2821 gcctggattt ctgaatgaac ttctgtcagg caaaactcag agaagaaaaa gccttagcct
     2881 caaaaacaag agtattacat ttccagagaa tgataaaagt tatagagaga tccctcagag
     2941 ttctgcagta gaaataaata atgaaactag gctggaagac agaaagggct tcagttttgt
     3001 gccattggca ggaacttcca aaccagtttt gtctgcatat gggcccgagg acatggagat
     3061 ttctataggt caaaccactg cttcagaata taaaactgtc ccaccagaag aaataactac
     3121 tatacctatg gataaaactg taatgtttgt agataatttc ggtgatctgg atgtcaccag
     3181 atcccatacg gttttcatcg attgtcaagc caaagaaaag gtacttgatg aatatactaa
     3241 cttgggaata cagaaaacaa aaactttgag tggttctgaa ggtgataccc atattcaaga
     3301 aatcacaaaa aacccagcag ctcaacacaa acaccatatg acaactgtta ttccttctag
     3361 tacagtagtt agtgatcaaa gttcaatgaa aatcaagttt cacaaagctg atagagatga
     3421 ggaggtcaaa ggaaaggaag tggaagccaa tatgctgaaa caaactaaac ctgaaagctg
     3481 tctgttaaat ataacagatg gaaaaaatgt ggattttaca agcagttata cagctgatgt
     3541 ttgtagatcc agtgacaagt attcctcttt accaaatatt tcttcttctg ataattcggg
     3601 tggtaatact atgtccttgt gtgataaaaa taaggagaaa gcttataatt gccaagtgcc
     3661 aaatgaattt acttatgcag ctatcttacc aagtacatat cacatggatt ctaaaaaact
     3721 gtctgtcttt ccaccttgtc cttcaaaaga agtcactcaa actgaaagtg ccatagcgct
     3781 gcttaaagat gaagatccgg tagaagagcc tctgggggag atggctacat ttaactcaaa
     3841 gcatgtatct cttaacctgg caaaggatca aacagaggcc tttgttgatg ttagtgttgc
     3901 ttctcagcct catctctcag cccaacaaag tccatcaact caaaaagggc aggatgttgc
     3961 ccgtagagat gaaggaatat tagctaaagc tgggaagaag gctttacctt ttcttctaga
     4021 aaatgtcgct gcatccacat gggaaaatga gtccaaaata cccactaatg tggaacactt
     4081 tgctgtaacc tatgaaaaag aactgagtat aagtattcaa acagataaat gtaatacaaa
     4141 tgtacagagc cctagtaact cggccctgac aacacaagtc attcagacgc atgcaaatgc
     4201 tgaaggagca ctagactttt tagttccatc tactgtttca tgttttagta gtaccaaacc
     4261 aagtctgagt aatttgaata gaaaaactga ggaggtctta gactttcaga ctgttaattt
     4321 attacctcct gccgaacagt tacttgaaga aggaagtcag gcacatagta tgagtatagt
     4381 acaagctaca gaaatatata gactaggttc caggaatgat agagatgaag aaagtaaaac
     4441 tttctgtaat gaggctgaaa ctacatctgt accactaaaa acagctgtta aagacaaaac
     4501 aagaagatgt tcattgggga tatttttacc caaattacca agcaagagaa gttgtagtat
     4561 cactggtgtt gatgacctgg aacagattct agcagatgca gctgacttaa ctcaactaga
     4621 aactcagcca gtctgtagta aagatccagg cattggatct gttgcagcca aactgaattt
     4681 gagtccatct cagtttataa atgaggaaaa tctccctgta tatcctggtg agatactttc
     4741 atcagattct gttagcctgg acattgagga aagtgttctg attgacacat ctcagagaga
     4801 gagtttaccg tctgaaaaca aaaccgaaaa ctgcagggcc caaaaaagaa cccgggttga
     4861 agaaaatgat gttacaaatg agaaaaaaat cagaacacat gacagtgcac aagatcaaga
     4921 gatttttgat aatcatatgg aagaggatat caataaaaat gtcaacagtg tattgctgaa
     4981 aagcctgagc aggacaccgt ctagttgcag cagctctctg gattcaatca agtctgatgg
     5041 actctctctg gatgtcagca cacagcgtaa tagccaaatg gaatcacagt ttcttggtga
     5101 cactatctct gaagagagtt tgaaggagaa actcaaggat ggacaaatta caataaagga
     5161 gttcttcata cttcttcagg tccacatctt aatacagaaa cctaggcaga gcactctccc
     5221 agccaaattt actataaaca cattacctac cacagaagat ctgatgttga gacaatatgt
     5281 ttatggaccc aggatacaga tttataaaga agactgtgag cttcttcgac aaaagattga
     5341 cgaattaaag atatctgcat tgaaccaaaa taaactattg gcggatgtaa ataggaactt
     5401 gtgggaaaaa gtgaaagact actctgatga agagctgaag aactatggga tttaccttaa
     5461 caaaatcaaa tcacgttata ccaagatgac aaaagtcttc aatcaccaag gaaaagtggc
     5521 tctgtataac aaattggtgc attcagctga gaatgagaag aacaaacttc agataaaaat
     5581 aaatgagatg gacactatcc tcaagaagat caataactgt ctcgctgaag tggaaacaga
     5641 aactaagaat ttggagaatg aagagaaaaa tgatgccatg gaagaatggg attctgaaat
     5701 gagagatgca gagaaagaac tggaacagct gaaaactgaa gaagaggagc ttcagagaaa
     5761 gttcttagaa gtagagacac agaaaacgca gacccttgct cagatagaat ttataaaaga
     5821 acaaacaact aaaactgaag aattactgga tcaattgagt ttgtctgagt gggatgtcat
     5881 tgagtggagt gatgatcaag ctgtatttac ctttgtctat gattcaatag aactcatcat
     5941 cacctttgga gagccactag ttggtctccc ttttttggac aaggcttgca ggaagattaa
     6001 tgcactcagt tttcagtctc tgttagatga agataaagct cctccctcct cccttttagt
     6061 tcacaagctt atttttcagt acattgagga acaggaatcc tggaagaaga aatgcacagc
     6121 acagcatcag gtgccccaga tgcttcaaga actctcactg gtagtgaacc attgtaggct
     6181 tcttggagag gagatcgaat ttttaaagcg atgggggcca aattatagcc taatgcatat
     6241 aaatgtgaat aatactgaat tgaggctttt attctccagt tgtgcagcat ttgcaaagtt
     6301 tgagataact ttgtctccct cggcccatta tcccttggtc ccgttaccgt tcaccattca
     6361 caatcacatt gggaagactg gccatgatga aattgctgca attatctcta aagtgccctt
     6421 agaggaaaac tacctgaaga atgtagtcaa gcaaatttac caagatctac ttaaggactg
     6481 aaagttttcc cgtaagaccc acagatcaca gctataacaa caaaggaaaa aaaaatccat
     6541 ttacccaggc cccatataga ggcaaacgct tataatctca ggattccagt gagagaagat
     6601 caaggctagc ctgggctgtc taggagacct ggttgaaaaa caaaatactt aaatgatata
     6661 tcggggtcaa tattgatatt tatcttttgt cttaacatca tataaaagta gcaaaattct
     6721 gccaggtggt ggtggtacac gcctttaatc ccagcacttg ggaggcagag gcaggcggat
     6781 ttctgagttc gaggccagcc tggtctacag agtgagttcc agatcagccc aggctataca
     6841 gagaaaccct gtcttgaaaa acaaaaacaa aacaaacaaa aatgcaaaat tcctcctgat
     6901 gatatcataa ttaatttgta tcattagttt taaaatacct agttgaaaat gatgaggtct
     6961 caaatagcat gttatctgct tgctattatc cttataccca agattaaatg atcaaacatg
     7021 tgagaaaatt ttcctaaagt agcttgttga tagaccactt accttgtgtg tataaagccc
     7081 tattttttaa aaagtctaaa gttgtgctct tctcttaata caagctctaa ggggtaacat
     7141 ttataaggag gtagtgcaca aagattaagc ctttcctctt tttgggtttt ttttgggggg
     7201 ggggggtgtt tggtgttgtt tttatttgtt ttggtttttg gtttttcgag acaggatgtc
     7261 tctgtgtagc cctggctgtc ctggaactca ctttgtagac caggctggcc ttaaactgag
     7321 aaatctacct gcctctgcct tccaagtgct gggattaaag gcatgcgcca ccactgccta
     7381 gctaatcatt tcttgttatc tagagctgtg tatttatggg ttttgcctag cagatatgtc
     7441 tagagtggaa acacttctat caggttctgg tagaacatat ctatggctgc agatactcag
     7501 atcactgaaa gagggagacc acatgatctc atgacttcaa gacgagcacg gtcaacatag
     7561 ccagggagat ttgtctccat aacaccaaca gaacatatct attcacaaat tattcattct
     7621 tctccatttc agaaatgctg tttaaaaaaa aaaactactc aacttttgga atggttgcca
     7681 tcttgcaagt cttagtattt ttttacaatg tttattaatg ttttctcttt ctaatgtata
     7741 ttctttttat ttctcatgta tactctttct aggaaaagat cattcatttt ggcctgatat
     7801 attatgtaaa tatatgaata ttttgtattt gtaatatatg tggtaatgaa ataatttgcc
     7861 ttcggtatag gtcatttcct tatctataaa gaatgcttta caggccgggc gtggtagcgc
     7921 atgcctttgt tttgttttgt tttgttttgt tttgttttgt tttgttttgt ttttccgaga
     7981 cagggtttct ttatgtagcg ctggctgtcc tagaactcac tctgtagacc aggctggcct
     8041 ccaactcaga aatctgcctg cctctacctc tgcctctgcc tcctgagtgc tgggattaaa
     8101 gcactggctt caatcttttt ttaataacaa aatttaaaca atatcacatt gctatacaag
     8161 tttctcacaa tcattctaaa ctattgcttt ctgaaaccac acttttactc atatacaaga
     8221 acaatacata gattgtttaa taaattaaca gtattgtctc tgaggtagac aaatgtgaac
     8281 gaactttccc cctacatgct agtcaattct taatcatggt aacttttgtt tcttttatat
     8341 ttaattctaa actgtgttta catcttaaaa ttaatttatc ttaaattttt actttttata
     8401 tcatggtaaa gtccttttag tcccacaaat gttttttatc tttttttttt tttgaaagtt
     8461 tttacttatg atctatacat caaaaggtta cacactctaa tggctttcaa aagtcaacac
     8521 aaaactcttt tgacttagaa attaagccaa atcgcttctc ggcctcaaga aattaagcca
     8581 agatacccgt aaaaatcttt taaagtcatc agtatggttt gtttcctatg agatttaatt
     8641 catccttttt cctgtatagc taaaaattag aggttatatt ttttccttaa acttgaaaaa
     8701 actgctccaa gaatttctct tataataatg gtgtctgttt tttggctttg gataatgtaa
     8761 ttatgtttat ataggtgttt gtatttttga atatttatag ttttggcaaa tttcatattt
     8821 tataagtttt tctttttttt cattttaaag gatttttttc attattaaag tacattgatt
     8881 acttgtaatc ctttataatt agtatctcta ttaagagtac tctgtagtgg ggctggagag
     8941 gtggctcagc aattagtacc tgctgtgtct agcagcctta atccagcttt catctcagga
     9001 gacagaggct gatgaatccc caagttcaag gctggccagg tttatagaag gagttccagg
     9061 acagccaggg ctacacagag aaaccctgtc tcggagagga gaaggaaaac aaacaaacac
     9121 ttgctggtct tgtagagtta gttcccagta cccacgtgat agctcgtaac tgtctgtaac
     9181 tcccgttcta gctcatgcaa tacctcttcc agcctccatg agcactgggc tcgcacaagg
     9241 cacacatgcc tacactgact aaaacaccca tatacatgaa ataaaaagga gtcttttttt
     9301 gttttggctt ttggtttttc aagacagggt ttctctgtat agccttggct gtcctggaac
     9361 tcactttgta gactaggctg gccttgaact cagaaatctg cctgcctttg cctcctgagt
     9421 gctggtatca aaggcgtgca tcaccacacc cggctgtttt gttttgtttt cttttctttt
     9481 cttttttttt tcattaattt gttttctttt ggtttttttt ttttttaaag atttatttat
     9541 ttattatatg taagtacact gtagctgtct tcagacactc cagaagaggg tgtcagattt
     9601 tgttacagat ggttgtgaac caccatgtgg ttgctgggat ttgaactccg gacctttgga
     9661 agagcagtcg ggtgctctta cccactgagc catctcacca gccccctttg ttttctttta
     9721 agggtatttt tgcctggtat agtggtgcag atctttaatc ctagcattca ggaggcagag
     9781 gcaggtggat ctctgaattc aagagaccag cctggtctac agagcctagt cccaaggata
     9841 actagagcta tacagagaaa ccctgtctcc acaaaatcaa acaggcacac acacacacac
     9901 acacacacac acacacacac acacacacac acacacacac acacccctat acttaacctt
     9961 cagtattacc aaaaagaaaa ggggaaatac attagccttt tatgtggcac aagtctgtaa
    10021 tgcctgtcct tgaaacctca aaaggtcatc tttagctaca tgacaagttc cataacagcc
    10081 tggatacaaa agttgctgtc tcaaaaatca aataattaag aattaaaagc tagatggagc
    10141 tagtgattta acttatttgt tagaatgcac acatgcctac actggctaaa acactcatat
    10201 acatgaaata aaaatgagtc tttttttgtt tttgttttgt tttttggttt ttcgaaacag
    10261 ggtttctctg tatagccttg gctgtcctgg aacttacttt gtagaccagg ctggcctcaa
    10321 actcagaaat ccgcctgcct ctgcctccta agtgctggga ttaaaggcct atgccaccac
    10381 cacaccagct tacagaaagt atatgtagac catgctgtcc acaaattcag agacccatct
    10441 ccctctacct cccaaaatgc ttcctgccta taatcccagc acttgaaaag tggacacaga
    10501 tgatcaggag tttaaaatca atatctcctg taagtattcc ttggatggaa ggatatcctg
    10561 ggagggaagg gatgaatcca ggagtggttg cctctgctcc tgggagagga tagaagggca
    10621 ctagacaagg cggttggggg cagcttatat aggatttcat agagggtggg gcttttcagg
    10681 gcagaggttt ttatttttat ttttttggtt tttcgagaca gggtttctct atgaagccct
    10741 ggctgtcctg gaactcactt tgtagaccag gctggcctgg aactcagaaa tctgcctgcc
    10801 tctgccaccc gagtgctggg attaaaggca tgcgccacca acgcccagct cagggcagag
    10861 attttgattt gacagttggt aagacttcaa gtcctgcgtt tggggagctc agggactggt
    10921 gggcttccct gtccttggtt tggtgggttt ttgtgagaag tacagagatt ggtaggtttt
    10981 ttggctcaag gattggtggc tttttttttt ccaccctctc ttttctgcat caggttcatg
    11041 ggttaggaat ggctttggct gaggtaccac ctctgtggag ctactggaga ggagacattg
    11101 ccaccgcgga cagctcctgt ggtgctaatt catgaagttc ataggctgag gcaggaaagg
    11161 tgccaccacc atggacagct cctacgactg cacctctagc tagtagcagg cagaattggg
    11221 aaaggatgtg ttgccacagt ggaaagctac tcgtgacagt atcagggtaa gacatggctg
    11281 tttggcaggt actggcattt taacagctca tgttgaggca tcttatgggt aggttggagc
    11341 aggaagaagg agccagcaac tgctttgaca gcttgcccag agtctacatc tactacacac
    11401 caagtcgaag gccagcctgg gctacaggag atcctatttc aaaaacagag accgaggatt
    11461 tcattcagga gagtgttcat ctagcatgca caaaaccctg ggtttgatcc taagcatctg
    11521 tctaaactta atgtggtgca ccacagtggt tatacttttt attgtcattc agacgccttg
    11581 tcaagttctt ccaggccata gccaccgcca gttctcccta ttcacaaatt gtgatatgag
    11641 caaacacagt tgtacacaag agattatcct aacatctctc ctttatagtc caataaaagt
    11701 ctaatttctc taatgccaat taactacata caataagaac aataatgaga actgtcaggt
    11761 aggaattaca ttcagtgtct aatcatctct gtttggcaac tttggaaaaa gtaccccact
    11821 atcctaactt ggtgaatcta aagttctgta tttaaatcac tttctaccat aattgtatga
    11881 atcaacctaa aaacatcatt attctcattg tatgataata tcagttgtgc tgaggtctgg
    11941 ggccctgtta acagtagcaa tgaaaagatg gctagctatg cagctatcct aacaccattt
    12001 aataaaatat tttaccacta ttgattggtg atgccttgat tatcatatac taaattgtta
    12061 gttttttatc atttcagttt gtaggaggca gaacaatgaa cccttaatgt ctacatttgt
    12121 aaccccagaa atgtaataat gttactctgt ttagccatag aggttttgca gatatattag
    12181 ctgggcaggc atgctatcac atacctgtta atcccagcac tcaggaggct aagcagagag
    12241 gattgttccc taggcaacat actgagactg cctttaaaaa gcaaaataaa attcagagag
    12301 aagattacat tcctgctgat tcaaatctga ttgagcttgc ttccctggaa cttcaggtca
    12361 ttgggttaca gatgtgggtc agttgaatac ttgcctagtt caggaactca gagtttgatg
    12421 ctctgctcag tactacatat accagtcata gtggtaagtt ccagtagtcc caacactcag
    12481 aagggtccaa gttccaagta tcttcatcta caatgcaaat ttgaggtaag cctgggctac
    12541 atataccaat taaagaacag acatgactaa gattgtatag ttcaatattt ttccaattac
    12601 agttatattt ttaggaattg ttggttctct tttagaattc tagttcatct tttttcccag
    12661 ttcttaggtt tttggttttt gaagaaaagt actcagacta gtttaagcat tttgggatgt
    12721 atgggttgtg ctaaattacc aaaagcaaca cccatgctgc tgggcctctg aagaagtgga
    12781 aagatctgaa aagtgataag aacgaaaagc aagtttttgg gtcttggggt atttgtttct
    12841 tctacccctt tctatctcaa tttaactgag aatgaataaa ccttttcttg tttgacagcc
    12901 a
//
