LOCUS       NM_008940               1326 bp    mRNA    linear   ROD 28-MAY-2024
DEFINITION  Mus musculus kallikrein related-peptidase 8 (Klk8), transcript
            variant 1, mRNA.
ACCESSION   NM_008940
VERSION     NM_008940.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Barman,B. and Thakur,M.K.
  TITLE     Neuropsin promotes hippocampal synaptogenesis by regulating the
            expression and cleavage of L1CAM
  JOURNAL   J Cell Sci 137 (3) (2024)
   PUBMED   38206094
REFERENCE   3  (bases 1 to 1326)
  AUTHORS   Xu,D.H., Du,J.K., Liu,S.Y., Zhang,H., Yang,L., Zhu,X.Y. and
            Liu,Y.J.
  TITLE     Upregulation of KLK8 contributes to CUMS-induced hippocampal
            neuronal apoptosis by cleaving NCAM1
  JOURNAL   Cell Death Dis 14 (4), 278 (2023)
   PUBMED   37076499
  REMARK    GeneRIF: Upregulation of KLK8 contributes to CUMS-induced
            hippocampal neuronal apoptosis by cleaving NCAM1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1326)
  AUTHORS   Herring,A., Kurapati,N.K., Krebs,S., Grammon,N., Scholz,L.M.,
            Voss,G., Miah,M.R., Budny,V., Mairinger,F., Haase,K.,
            Teuber-Hanselmann,S., Dobersalske,C., Schramm,S., Jockel,K.H.,
            Munster,Y. and Keyvani,K.
  TITLE     Genetic knockdown of Klk8 has sex-specific multi-targeted
            therapeutic effects on Alzheimer's pathology in mice
  JOURNAL   Neuropathol Appl Neurobiol 47 (5), 611-624 (2021)
   PUBMED   33341972
  REMARK    GeneRIF: Genetic knockdown of Klk8 has sex-specific multi-targeted
            therapeutic effects on Alzheimer's pathology in mice.
REFERENCE   5  (bases 1 to 1326)
  AUTHORS   Du,J.K., Yu,Q., Liu,Y.J., Du,S.F., Huang,L.Y., Xu,D.H., Ni,X. and
            Zhu,X.Y.
  TITLE     A novel role of kallikrein-related peptidase 8 in the pathogenesis
            of diabetic cardiac fibrosis
  JOURNAL   Theranostics 11 (9), 4207-4231 (2021)
   PUBMED   33754057
  REMARK    GeneRIF: A novel role of kallikrein-related peptidase 8 in the
            pathogenesis of diabetic cardiac fibrosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1326)
  AUTHORS   Kishi,T., Kato,M., Shimizu,T., Kato,K., Matsumoto,K., Yoshida,S.,
            Shiosaka,S. and Hakoshima,T.
  TITLE     Crystal structure of neuropsin, a hippocampal protease involved in
            kindling epileptogenesis
  JOURNAL   J Biol Chem 274 (7), 4220-4224 (1999)
   PUBMED   9933620
REFERENCE   7  (bases 1 to 1326)
  AUTHORS   Shimizu,C., Yoshida,S., Shibata,M., Kato,K., Momota,Y.,
            Matsumoto,K., Shiosaka,T., Midorikawa,R., Kamachi,T., Kawabe,A. and
            Shiosaka,S.
  TITLE     Characterization of recombinant and brain neuropsin, a
            plasticity-related serine protease
  JOURNAL   J Biol Chem 273 (18), 11189-11196 (1998)
   PUBMED   9556608
REFERENCE   8  (bases 1 to 1326)
  AUTHORS   Chen,Z.L., Momota,Y., Kato,K., Taniguchi,M., Inoue,N., Shiosaka,S.
            and Yoshida,S.
  TITLE     Expression of neuropsin mRNA in the mouse embryo and the pregnant
            uterus
  JOURNAL   J Histochem Cytochem 46 (3), 313-320 (1998)
   PUBMED   9487112
REFERENCE   9  (bases 1 to 1326)
  AUTHORS   Suzuki,J., Yoshida,S., Chen,Z.L., Momota,Y., Kato,K., Hirata,A. and
            Shiosaka,S.
  TITLE     Ontogeny of neuropsin mRNA expression in the mouse brain
  JOURNAL   Neurosci Res 23 (4), 345-351 (1995)
   PUBMED   8602273
REFERENCE   10 (bases 1 to 1326)
  AUTHORS   Chen,Z.L., Yoshida,S., Kato,K., Momota,Y., Suzuki,J., Tanaka,T.,
            Ito,J., Nishino,H., Aimoto,S., Kiyama,H. et al.
  TITLE     Expression and activity-dependent changes of a novel limbic-serine
            protease gene in the hippocampus
  JOURNAL   J Neurosci 15 (7 Pt 2), 5088-5097 (1995)
   PUBMED   7623137
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC151989.4, AB074296.1 and
            EH106421.1.
            
            On May 3, 2016 this sequence version replaced NM_008940.2.
            
            Summary: This gene encodes a member of the kallikrein subfamily of
            serine proteases that are involved in diverse physiological
            functions such as skin desquamation, tooth enamel formation,
            seminal liquefaction, synaptic neural plasticity and brain
            function. The encoded preproprotein undergoes proteolytic cleavage
            of the activation peptide to generate the functional enzyme. Mice
            lacking the encoded protein exhibit impaired long-term potentiation
            and increased anxiety, as well as a hyperkeratosis phenotype. This
            gene is located in a cluster of several related kallikrein genes on
            chromosome 7. [provided by RefSeq, May 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D30785.1, BC055895.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-556               AC151989.4         20018-20573         c
            557-716             AC151989.4         19367-19526         c
            717-738             AC151989.4         19064-19085         c
            739-1321            AB074296.1         1-583
            1322-1326           EH106421.1         36-40               c
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.26 cM"
     gene            1..1326
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /note="kallikrein related-peptidase 8"
                     /db_xref="GeneID:259277"
                     /db_xref="MGI:MGI:1343327"
     exon            1..556
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /inference="alignment:Splign:2.1.0"
     CDS             487..1269
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /EC_number="3.4.21.118"
                     /note="kallikrein-8; Brain Serine protease 1; protease,
                     serine, 19 (neuropsin)"
                     /codon_start=1
                     /product="kallikrein-8 preproprotein"
                     /protein_id="NP_032966.1"
                     /db_xref="CCDS:CCDS21182.1"
                     /db_xref="GeneID:259277"
                     /db_xref="MGI:MGI:1343327"
                     /translation="MGRPPPCAIQPWILLLLFMGAWAGLTRAQGSKILEGRECIPHSQ
                     PWQAALFQGERLICGGVLVGDRWVLTAAHCKKQKYSVRLGDHSLQSRDQPEQEIQVAQ
                     SIQHPCYNNSNPEDHSHDIMLIRLQNSANLGDKVKPVQLANLCPKVGQKCIISGWGTV
                     TSPQENFPNTLNCAEVKIYSQNKCERAYPGKITEGMVCAGSSNGADTCQGDSGGPLVC
                     DGMLQGITSWGSDPCGKPEKPGVYTKICRYTTWIKKTMDNRD"
     exon            557..716
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /inference="alignment:Splign:2.1.0"
     exon            717..979
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1113
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1326
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1300..1305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /note="hexamer: AATAAA"
     polyA_site      1322
                     /gene="Klk8"
                     /gene_synonym="BSP1; NP; Nrpn; Prss19"
                     /note="major polyA site"
ORIGIN      
        1 gacacaccga agggaagtcc gggggcctct tccaccgagt ccgagtgacc ccgccccttg
       61 cattctggaa ggtgaggcgc agaggtcccc agacacggac ctcaggcgca gggaggtccc
      121 ctttctctga gcccaggacc ctcccacccc caggctcaca ttctttctct caggatcttc
      181 aagcgggtct cttaagctcc ctcttcccca ggacgttgga gtcacagcct cagatctttc
      241 tctccaatct cacaagtggg ccagaactcc tttataatgt ctggatcccc aacagcaagc
      301 tctcccccac actaaaattc ggggatctag agctctgccc tagctttctc agcccctagc
      361 tccatcctcc agcaagactc aagacagctc cggaaacacc tccttccccc agttccccag
      421 acaacaagat ctcaggctcc tccctcggac ttcctcttag ttccaccctc ttcctcagag
      481 gccaccatgg gacgcccccc accctgtgca atccagccgt ggatccttct gcttctgttc
      541 atgggagcgt gggcagggct caccagagct cagggctcca agatcctgga aggtcgagag
      601 tgtatacccc actcccagcc ttggcaggca gccttgttcc agggcgagag actgatctgt
      661 gggggtgtcc tggttggaga cagatgggtc ctcacggcag cccactgcaa aaaacagaag
      721 tactccgtgc gtctgggtga tcatagcctc cagagcagag atcagccgga gcaggagatc
      781 caggtggctc agtctatcca gcatccttgc tacaacaaca gcaacccaga agatcacagt
      841 cacgatataa tgctcattcg actgcagaac tcagcaaacc tcggggacaa ggtgaagccg
      901 gtccaactgg ccaatctgtg tcccaaagtt ggccagaagt gcatcatatc aggctggggc
      961 actgtcacca gccctcaaga gaactttcca aacaccctca actgtgcgga agtgaaaatc
     1021 tattcccaga acaagtgtga gagagcctat ccagggaaga tcaccgaggg catggtctgt
     1081 gctggcagca gcaatggagc tgacacgtgc cagggtgact caggaggccc tctggtgtgc
     1141 gacgggatgc tccagggcat cacctcatgg ggctcagacc cctgtgggaa acccgagaaa
     1201 cctggagtct acaccaaaat ctgccgctac actacctgga tcaagaagac catggacaac
     1261 agggactgat cctggtgtgt gtgtgtgggg ggggttgtca ataaacacca ccattggctg
     1321 gctcct
//
