LOCUS       NM_008457                860 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus kallikrein 1-related peptidase b8 (Klk1b8), mRNA.
ACCESSION   NM_008457
VERSION     NM_008457.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lundwall,A., Band,V., Blaber,M., Clements,J.A., Courty,Y.,
            Diamandis,E.P., Fritz,H., Lilja,H., Malm,J., Maltais,L.J.,
            Olsson,A.Y., Petraki,C., Scorilas,A., Sotiropoulou,G.,
            Stenman,U.H., Stephan,C., Talieri,M. and Yousef,G.M.
  TITLE     A comprehensive nomenclature for serine proteases with homology to
            tissue kallikreins
  JOURNAL   Biol Chem 387 (6), 637-641 (2006)
   PUBMED   16800724
REFERENCE   2  (bases 1 to 860)
  AUTHORS   Angata,T., Margulies,E.H., Green,E.D. and Varki,A.
  TITLE     Large-scale sequencing of the CD33-related Siglec gene cluster in
            five mammalian species reveals rapid evolution by multiple
            mechanisms
  JOURNAL   Proc Natl Acad Sci U S A 101 (36), 13251-13256 (2004)
   PUBMED   15331780
REFERENCE   3  (bases 1 to 860)
  AUTHORS   Olsson,A.Y., Lilja,H. and Lundwall,A.
  TITLE     Taxon-specific evolution of glandular kallikrein genes and
            identification of a progenitor of prostate-specific antigen
  JOURNAL   Genomics 84 (1), 147-156 (2004)
   PUBMED   15203212
REFERENCE   4  (bases 1 to 860)
  AUTHORS   Olsson,A.Y. and Lundwall,A.
  TITLE     Organization and evolution of the glandular kallikrein locus in Mus
            musculus
  JOURNAL   Biochem Biophys Res Commun 299 (2), 305-311 (2002)
   PUBMED   12437987
REFERENCE   5  (bases 1 to 860)
  AUTHORS   Chen,Z.L., Yoshida,S., Kato,K., Momota,Y., Suzuki,J., Tanaka,T.,
            Ito,J., Nishino,H., Aimoto,S., Kiyama,H. et al.
  TITLE     Expression and activity-dependent changes of a novel limbic-serine
            protease gene in the hippocampus
  JOURNAL   J Neurosci 15 (7 Pt 2), 5088-5097 (1995)
   PUBMED   7623137
REFERENCE   6  (bases 1 to 860)
  AUTHORS   Evans,B.A., Drinkwater,C.C. and Richards,R.I.
  TITLE     Mouse glandular kallikrein genes. Structure and partial sequence
            analysis of the kallikrein gene locus
  JOURNAL   J Biol Chem 262 (17), 8027-8034 (1987)
   PUBMED   3036794
REFERENCE   7  (bases 1 to 860)
  AUTHORS   Fahnestock,M., Brundage,S. and Shooter,E.M.
  TITLE     The sequence of a cDNA clone coding for a novel kallikrein from
            mouse submaxillary gland
  JOURNAL   Nucleic Acids Res 14 (12), 4823-4835 (1986)
   PUBMED   3636812
REFERENCE   8  (bases 1 to 860)
  AUTHORS   van Leeuwen,B.H., Evans,B.A., Tregear,G.W. and Richards,R.I.
  TITLE     Mouse glandular kallikrein genes. Identification, structure, and
            expression of the renal kallikrein gene
  JOURNAL   J Biol Chem 261 (12), 5529-5535 (1986)
   PUBMED   3007510
REFERENCE   9  (bases 1 to 860)
  AUTHORS   Mason,A.J., Evans,B.A., Cox,D.R., Shine,J. and Richards,R.I.
  TITLE     Structure of mouse kallikrein gene family suggests a role in
            specific processing of biologically active peptides
  JOURNAL   Nature 303 (5915), 300-307 (1983)
   PUBMED   6602295
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC134858.6.
            
            On Feb 23, 2016 this sequence version replaced NM_008457.2.
            
            Summary: This gene encodes a member of the kallikrein subfamily of
            serine proteases that are involved in diverse physiological
            functions such as skin desquamation, tooth enamel formation,
            seminal liquefaction, synaptic neural plasticity and brain
            function. The encoded preproprotein undergoes proteolytic cleavage
            of the activation peptide to generate the functional enzyme. This
            gene is located in a cluster of several related kallikrein genes on
            chromosome 7. [provided by RefSeq, Feb 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031798.1, X03994.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                AC134858.6         140764-140834
            72-231              AC134858.6         142790-142949
            232-518             AC134858.6         143949-144235
            519-655             AC134858.6         144332-144468
            656-860             AC134858.6         144838-145042
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.43 cM"
     gene            1..860
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /note="kallikrein 1-related peptidase b8"
                     /db_xref="GeneID:16624"
                     /db_xref="MGI:MGI:892018"
     exon            1..71
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..811
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /EC_number="3.4.21.35"
                     /note="tissue kallikrein-8; glandular kallikrein K8;
                     pseudo-kallikrein"
                     /codon_start=1
                     /product="kallikrein 1-related peptidase b8 preproprotein"
                     /protein_id="NP_032483.1"
                     /db_xref="CCDS:CCDS21189.1"
                     /db_xref="GeneID:16624"
                     /db_xref="MGI:MGI:892018"
                     /translation="MRFLILFLALSLGGIDAAPPLQSRVVGGFNCEKNSQPWQVAVYD
                     NKEHICGGVLLERNWVLTAAHCYVDQYEVWLGKNKLFQEEPSAQHRLVSKSFPHPGFN
                     MSLLTLKEIPPGADFSNDLMLLRLSKPADITDAVKPITLPTKESKLGSTCLASGWGSI
                     TPTKWQKPDDLQCVFLKLLPIKNCIENHNVKVTDVMLCAGEMSGGKNICKGDSGGPLI
                     CDSVLQGITSTGPIPCGKPGVPAMYTNLIKFNSWIKDTMTKNS"
     exon            72..231
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /inference="alignment:Splign:2.1.0"
     exon            232..518
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /inference="alignment:Splign:2.1.0"
     exon            519..655
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /inference="alignment:Splign:2.1.0"
     exon            656..860
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      835..840
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /note="hexamer: AATAAA"
     polyA_site      857
                     /gene="Klk1b8"
                     /gene_synonym="kallikrein; Klk8; mGK-8; Nrpn; TADG14"
                     /note="major polyA site"
ORIGIN      
        1 ctgctgctcc tggacacctg ttaccatgag gttcctgatc ctgttcctag ccctgtccct
       61 aggagggatt gatgctgcac ctcctctcca gtctcgggtg gttggaggat ttaactgtga
      121 gaagaattcc caaccctggc aggtggctgt gtatgacaac aaggaacaca tttgtggggg
      181 agtcctgttg gaacgcaact gggttctcac agctgcccac tgctatgtcg accagtatga
      241 ggtttggctg ggcaaaaaca agttattcca agaggaaccc tctgctcaac accgattggt
      301 cagcaaaagc ttccctcacc ctggcttcaa catgagcctc ctgacactca aagagatacc
      361 tcctggggct gacttcagca atgacctgat gctgctccgc ctcagcaagc ctgctgacat
      421 cacagatgct gtgaagccca tcaccctgcc cacgaaggag tcaaaactgg ggagcacatg
      481 cctagcctca ggctggggca gcattacacc cacgaaatgg caaaagccag atgatctcca
      541 gtgtgtgttc ctcaagctcc tgcctattaa gaactgtata gaaaaccaca atgtgaaggt
      601 gacagatgtc atgctgtgtg caggagagat gagtggaggc aaaaacattt gcaagggtga
      661 ctccggaggc ccattgatct gtgatagtgt tctccaaggt atcacatcaa caggccctat
      721 accatgcggt aaacctggtg taccagccat gtacaccaac cttattaagt tcaactcctg
      781 gataaaagat actatgacga aaaattcctg agtgtcacat tatcccatgt tctcaataaa
      841 acccaccatg aagcaaatga
//
