LOCUS       NM_010639                958 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus kallikrein 1 (Klk1), transcript variant 1, mRNA.
ACCESSION   NM_010639
VERSION     NM_010639.8
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 958)
  AUTHORS   Desposito,D., Zadigue,G., Taveau,C., Adam,C., Alhenc-Gelas,F.,
            Bouby,N. and Roussel,R.
  TITLE     Neuroprotective effect of kinin B1 receptor activation in acute
            cerebral ischemia in diabetic mice
  JOURNAL   Sci Rep 7 (1), 9410 (2017)
   PUBMED   28842604
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 958)
  AUTHORS   Mo,Q., Salley,J., Roshan,T., Baer,L.A., May,F.J., Jaehnig,E.J.,
            Lehnig,A.C., Guo,X., Tong,Q., Nuotio-Antar,A.M., Shamsi,F.,
            Tseng,Y.H., Stanford,K.I. and Chen,M.H.
  TITLE     Identification and characterization of a supraclavicular brown
            adipose tissue in mice
  JOURNAL   JCI Insight 2 (11), 93166 (2017)
   PUBMED   28570265
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 958)
  AUTHORS   Murakami,K., Tanaka,T., Bando,Y. and Yoshida,S.
  TITLE     Differences in Kallikrein-related peptidase mRNA sequences and KLK6
            enzyme activity between mouse strains
  JOURNAL   Protein Pept Lett 22 (3), 204-211 (2014)
   PUBMED   25666037
REFERENCE   5  (bases 1 to 958)
  AUTHORS   Penschow,J.D. and Coghlan,J.P.
  TITLE     Sites of glandular kallikrein gene expression in fetal mice
  JOURNAL   Mol Cell Endocrinol 88 (1-3), 23-30 (1992)
   PUBMED   1334010
REFERENCE   6  (bases 1 to 958)
  AUTHORS   Peters,J., Takahashi,S., Tada,M. and Miyake,Y.
  TITLE     mGK-6-derived true tissue kallikrein is synthesized, processed, and
            targeted through a regulated secretory pathway in mouse pituitary
            AtT-20 cells
  JOURNAL   J Biochem 111 (5), 643-648 (1992)
   PUBMED   1639762
REFERENCE   7  (bases 1 to 958)
  AUTHORS   Hearne,C.M., McAleer,M.A., Love,J.M., Aitman,T.J., Cornall,R.J.,
            Ghosh,S., Knight,A.M., Prins,J.B. and Todd,J.A.
  TITLE     Additional microsatellite markers for mouse genome mapping
  JOURNAL   Mamm Genome 1 (4), 273-282 (1991)
   PUBMED   1794058
REFERENCE   8  (bases 1 to 958)
  AUTHORS   Saunders,A.M. and Seldin,M.F.
  TITLE     A molecular genetic linkage map of mouse chromosome 7
  JOURNAL   Genomics 8 (3), 525-535 (1990)
   PUBMED   1981054
REFERENCE   9  (bases 1 to 958)
  AUTHORS   Murakami,K., Ikigai,H., Nagumo,N., Tomita,M. and Shimamura,T.
  TITLE     A cytocidal tissue kallikrein isolated from mouse submandibular
            glands
  JOURNAL   FEBS Lett 257 (2), 400-402 (1989)
   PUBMED   2583286
REFERENCE   10 (bases 1 to 958)
  AUTHORS   Evans,B.A., Drinkwater,C.C. and Richards,R.I.
  TITLE     Mouse glandular kallikrein genes. Structure and partial sequence
            analysis of the kallikrein gene locus
  JOURNAL   J Biol Chem 262 (17), 8027-8034 (1987)
   PUBMED   3036794
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124176.3, BY087276.1,
            AK002278.1 and AV053449.1.
            
            On Feb 20, 2016 this sequence version replaced NM_010639.7.
            
            Summary: This gene encodes a member of the kallikrein subfamily of
            serine proteases that are involved in diverse physiological
            functions such as skin desquamation, tooth enamel formation,
            seminal liquefaction, synaptic neural plasticity and brain
            function. The encoded preproprotein undergoes proteolytic cleavage
            of the activation peptide to generate the functional enzyme. Mice
            lacking the encoded protein are unable to generate significant
            levels of kinins in most tissues, develop cardiovascular
            abnormalities and exhibit hypercalciuria of renal origin. This gene
            is located in a cluster of several related kallikrein genes on
            chromosome 7. Alternative splicing results in multiple transcript
            variants encoding different isoforms, some of which may undergo
            similar processing. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D10464.1, BC053697.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AC124176.3         160066-160099
            35-77               BY087276.1         2-44
            78-951              AK002278.1         2-875
            952-958             AV053449.1         276-282
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.74 cM"
     gene            1..958
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /note="kallikrein 1"
                     /db_xref="GeneID:16612"
                     /db_xref="MGI:MGI:102850"
     exon            1..165
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /inference="alignment:Splign:2.1.0"
     CDS             120..905
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /EC_number="3.4.21.35"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; true tissue kallikrein; tissue kallikrein-6;
                     glandular kallikrein K1; kallikrein
                     renal/pancreas/salivary"
                     /codon_start=1
                     /product="kallikrein-1 isoform 1 preproprotein"
                     /protein_id="NP_034769.4"
                     /db_xref="CCDS:CCDS21202.1"
                     /db_xref="GeneID:16612"
                     /db_xref="MGI:MGI:102850"
                     /translation="MRFLILFLALSLGGIDAAPPVQSRIVGGFNCEKNSQPWQVAVYR
                     FTKYQCGGILLNANWVLTAAHCHNDKYQVWLGKNNFLEDEPSAQHRLVSKAIPHPDFN
                     MSLLNEHTPQPEDDYSNDLMLLRLKKPADITDVVKPIDLPTEEPKLGSTCLASGWGSI
                     TPVKYEYPDELQCVNLKLLPNEDCAKAHIEKVTDDMLCAGDMDGGKDTCAGDSGGPLI
                     CDGVLQGITSWGPSPCGKPNVPGIYTRVLNFNTWIRETMAEND"
     exon            166..325
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /inference="alignment:Splign:2.1.0"
     exon            326..612
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /inference="alignment:Splign:2.1.0"
     exon            613..749
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /inference="alignment:Splign:2.1.0"
     exon            750..958
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      929..934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /note="hexamer: AATAAA"
     polyA_site      951
                     /gene="Klk1"
                     /gene_synonym="0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6;
                     mGk-6"
                     /note="major polyA site"
ORIGIN      
        1 catctggctg ccaacacggc aggggtgggg ctgtggggga gaatgagggt ttttaaaggc
       61 tccccaggag cctctacagc tccaagatca ctgcctgcag ctcctggact cctgttacca
      121 tgaggttcct gatcctgttc ctagccctgt ccctaggagg gattgatgct gcacctcctg
      181 tccagtctcg aattgttgga ggatttaact gtgagaagaa ttcccagccc tggcaagtgg
      241 ctgtgtaccg cttcaccaaa tatcaatgtg ggggtatcct gctgaacgcc aactgggttc
      301 tcacagctgc ccactgccat aatgacaagt accaggtgtg gctgggcaaa aacaactttt
      361 tggaggatga accctctgcc caacaccggc ttgtcagcaa agccatccct caccctgact
      421 tcaacatgag cctcctgaat gagcacaccc cacaacctga ggatgactac agcaatgacc
      481 tgatgctgct ccgcctcaaa aagcctgctg acatcacaga tgttgtgaag cccatcgacc
      541 tgcccactga ggagcccaag ctggggagca catgcctagc ctcaggctgg ggcagcatta
      601 cacccgtcaa atatgaatac ccagatgagc tccagtgtgt gaacctcaag ctcctgccta
      661 atgaggactg tgccaaagcc cacatagaga aggtgacaga tgacatgttg tgtgcaggag
      721 atatggatgg aggcaaagac acttgtgcgg gtgactcagg aggcccactg atctgtgatg
      781 gtgttctcca aggtatcaca tcatggggcc ctagcccttg cggtaaaccc aatgtgccgg
      841 gtatctacac cagagtttta aatttcaaca cctggataag agaaactatg gctgaaaatg
      901 actgagtatc acattgtccc atgttctcaa taaagcccac catgcagcaa atgagttc
//
