LOCUS       NM_178741               3193 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus kelch-like 8 (Klhl8), transcript variant 2, mRNA.
ACCESSION   NM_178741
VERSION     NM_178741.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3193)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 3193)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 3193)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
REFERENCE   6  (bases 1 to 3193)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 3193)
  AUTHORS   Isaacs,A.M., Oliver,P.L., Jones,E.L., Jeans,A., Potter,A.,
            Hovik,B.H., Nolan,P.M., Vizor,L., Glenister,P., Simon,A.K.,
            Gray,I.C., Spurr,N.K., Brown,S.D., Hunter,A.J. and Davies,K.E.
  TITLE     A mutation in Af4 is predicted to cause cerebellar ataxia and
            cataracts in the robotic mouse
  JOURNAL   J Neurosci 23 (5), 1631-1637 (2003)
   PUBMED   12629167
REFERENCE   8  (bases 1 to 3193)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY250847.1 and AK031028.1.
            
            On Jan 24, 2018 this sequence version replaced NM_178741.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. All three variants encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK031028.1, SRR1660817.144710.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BY250847.1         2-31
            31-3193             AK031028.1         2-3164
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 50.45 cM"
     gene            1..3193
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /note="kelch-like 8"
                     /db_xref="GeneID:246293"
                     /db_xref="MGI:MGI:2179430"
     exon            1..144
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            145..528
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     CDS             286..2175
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /codon_start=1
                     /product="kelch-like protein 8"
                     /protein_id="NP_848856.1"
                     /db_xref="CCDS:CCDS19479.1"
                     /db_xref="GeneID:246293"
                     /db_xref="MGI:MGI:2179430"
                     /translation="MASESTNGKQARSHVTKGRRQYQHQHQQQQQQQQQVRSRSSVSE
                     CDGDDSFIFEANEAWKDFHGSLLGFYENGELCDVTLKVGSKLISCHKLVLACVIPYFR
                     AMFLSEMSEAKQALIEIRDFDGDAVEDLVKFVYSSRLTLTVDNVQPLLYAACILQVEL
                     VARACCEYMQLHFHPSNCLAVRAFAESHNRIDLMDMADQYACEHFTEVVECEDFVSVS
                     PQHLHKLLSSSDLNIDSEKQVYSAAIKWLLANPQHHPKWLDETLAQVRLPLLPVDFLM
                     GVVAKEQIVKQNLKCRDLLDEARNYHLHLSSKPVPDFEYTVRTTPRKHTAGVLFCVGG
                     RGGSGDPFRSIECYSINKNSWFFGPEMNSRRRHVGVISVEGKVYAVGGHDGNEHLGSM
                     EMFDPLTNKWMMKASMNTKRRGIALASLGGPIYAIGGLDDNTCFSDVERYDIESDQWS
                     TVAPMNTPRGGVGSVALINHVYAVGGNDGVASLSSVERYHPHLDKWIEVKEMGQRRAG
                     NGVSELHGCLYVVGGFDDNSPLSSVERYDPRSNKWDYVAALTTPRGGVGIATVMGKIF
                     AVGGHNGNAYLNTVEAFDPVLNKWELVGPVSHCRAGAGVAVCDCLTSQIRDVGHGSTN
                     VVDCM"
     exon            529..1077
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1264
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1408
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1520
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1689
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1849
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..2051
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..3193
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2449..2454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /note="hexamer: AATAAA"
     polyA_site      2468
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /note="major polyA site"
     regulatory      3143..3148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /note="hexamer: AATAAA"
     polyA_site      3174
                     /gene="Klhl8"
                     /gene_synonym="2310001P09Rik; D5Ertd431e"
                     /note="major polyA site"
ORIGIN      
        1 ggctctcaag agcgctcggc cgagccgggc acgagcgagc ggtcgggccg gtgagcgcgt
       61 ccccggggtg tccaagcggg gctggcgcgg cgccggcgcg gagcgggtgt ggcggctcgg
      121 cctgcgcggc ggccgcggac agagcagact gtatcgtgac attgaaggaa ttttgaaaga
      181 tgcttcaagc ttagtagcca catctattgt tggtcttctc acccttaaaa agagaataaa
      241 tgggatgttt cagaagagag ctgtataaaa agaagagtat tgcaaatggc ttcagaatct
      301 accaatggga aacaagctag gagtcacgtg acaaaggggc ggcgtcagta ccagcaccag
      361 caccaacagc agcagcaaca gcagcagcaa gtgaggagca ggtcctctgt cagtgagtgt
      421 gatggcgacg actccttcat cttcgaagcc aacgaggcct ggaaggactt tcacggctct
      481 ctcctgggct tctatgaaaa cggggagctc tgcgacgtca ccctgaaggt tggctcaaag
      541 ctaatatctt gtcacaaact ggtgctggct tgtgtgatcc cctacttcag agccatgttt
      601 ctttccgaaa tgtctgaagc caagcaggcg ttaatcgaga tccgagactt tgacggtgat
      661 gccgtggaag acttggtgaa gtttgtctac tcctcacggc tcactttaac agtggacaat
      721 gtccagcccc tgctgtatgc agcgtgtatt ctgcaggttg agctggtggc cagagcttgt
      781 tgtgaataca tgcagctaca ctttcatccc tccaactgcc tggctgtacg agccttcgca
      841 gagagtcaca accgaataga cctaatggac atggcggacc agtatgcctg tgagcacttt
      901 actgaagtgg tggagtgcga agacttcgtg agtgtgtccc cgcaacatct ccacaagctc
      961 ctctcctcta gcgacctcaa cattgacagt gagaagcagg tctacagtgc tgccatcaag
     1021 tggcttcttg ccaatccgca acatcatcct aaatggctgg acgagacact tgcacaggtc
     1081 cgtctgccat tgctgccagt tgattttctc atgggtgttg tggcgaaaga acagatcgtc
     1141 aagcagaatc tgaagtgcag agatctgctg gacgaagcga gaaattacca ccttcacttg
     1201 agcagtaaac cagtcccgga ctttgagtac acagtcagga cgaccccgag gaagcacact
     1261 gctggtgtgc tgttttgtgt gggtggtcga ggtggatctg gtgacccctt ccgtagtatt
     1321 gaatgctatt ctatcaacaa aaacagctgg ttctttggac cagaaatgaa cagccgcagg
     1381 cgacatgtgg gtgtgatctc tgtggaaggg aaggtgtacg cagtgggtgg ccatgatggg
     1441 aacgaacacc tgggcagcat ggagatgttt gaccctctca ctaacaagtg gatgatgaag
     1501 gcatcgatga acaccaagag gcgaggaatt gccttggcct ccttaggggg cccaatatat
     1561 gcaattggag gattagatga caatacgtgc ttcagtgacg tggagagata cgacatcgaa
     1621 tctgaccaat ggagcacagt ggcaccaatg aacactcccc gtggaggcgt tggctctgtg
     1681 gctttaataa accacgttta cgcagtgggc ggcaacgacg gcgtagcctc cttatccagt
     1741 gtggagaggt atcacccgca tctggacaag tggatagaag tgaaagagat gggtcagcgc
     1801 agagctggca acggcgtcag cgagcttcac ggttgtttat atgttgttgg tggttttgac
     1861 gacaactccc ctttgagttc cgtggaacgg tacgaccctc gaagcaacaa gtgggactat
     1921 gtggcagctc ttaccactcc caggggtggc gtagggatcg caacagtgat gggcaaaatc
     1981 tttgcagttg gcggtcacaa tggcaacgcg tacctaaaca ccgtggaggc gtttgacccc
     2041 gtgctgaaca agtgggagct ggtcggacct gtgtctcact gccgagctgg tgccggtgtg
     2101 gccgtgtgtg actgtttaac tagccagatc cgagacgtgg gtcacgggtc cactaatgtg
     2161 gttgactgta tgtgatgaag tcccagcaac aaggacacaa tcccgtctga tggacaggaa
     2221 gacagcaggc cttttgactg tgactgcagc gactgttttt gaatgtcagc tgttgtgaat
     2281 acacgtctgt ggactgacgg gtgagagctg ttgtcataaa gccaggtgag ctctttcctg
     2341 cttcagcaaa cagtggagcg ggctggaagc agcccatctt gaacccattc ttgctctgag
     2401 agatgtttga tgtagtgcca aaccttaaaa tagctcttta ttttgatgaa taaaatgtaa
     2461 tttattgagt ttcatctatt ctttttcctg aagaaactaa cttcaggcag taggtttttc
     2521 ccagaggaga gagatgtcca ctcatcaaca tgagaactta aggcctgctc ccttgaggat
     2581 cattctacac ggtgctgacg aattcatgtc ttgctttttg tctctagatg gtgactttca
     2641 gttcttgggt gtttatgaag tagggaaaat gtttatgtgt ttctgaaaca ttcttgaaac
     2701 catattagtg tctgtaagaa ggccttcgct tccatttcga gtttcagaac tacaggacac
     2761 cactgtactg gcttcctcac gaagtctggt ggctgctatt cttttcccac ccgggagaat
     2821 ttcttgggat gactgtggat tagtgttagt gcagcgcccg gtgaatgaga tccgcgttcg
     2881 tgtgccgccc agtgaaaaga cagagggtgt gtgacgccca tgtccaccta gggagggaat
     2941 ggggaggttc cttgaacttc gttctttggg tttgtcgttg gatttggagc gaggatccat
     3001 tttctcttta ctctgtcatt tgtcactgtc ctggtttttc taattgtaca aagctgaatt
     3061 tatttattat caggacatga atgcattttt acaaaatttc cttctgcagt atagtttgat
     3121 tagttcaagt ttgtcattaa tcaataaaat gttgttggta aaagcagaaa aaaatggaaa
     3181 ctactagtct ttt
//
