LOCUS       NM_175174               3422 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus kelch-like 5 (Klhl5), transcript variant 1, mRNA.
ACCESSION   NM_175174
VERSION     NM_175174.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 3422)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
REFERENCE   4  (bases 1 to 3422)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC057606.1 and AI120540.1.
            
            On Feb 6, 2008 this sequence version replaced NM_175174.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057606.1, SRR1660817.150581.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3244              BC057606.1         1-3244
            3245-3422           AI120540.1         1-178               c
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 33.58 cM"
     gene            1..3422
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="kelch-like 5"
                     /db_xref="GeneID:71778"
                     /db_xref="MGI:MGI:1919028"
     exon            1..546
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..2293
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="kelch-like protein 5 isoform 1"
                     /protein_id="NP_780383.2"
                     /db_xref="CCDS:CCDS19305.2"
                     /db_xref="GeneID:71778"
                     /db_xref="MGI:MGI:1919028"
                     /translation="MSGSRKEFDVKQILKIRWRWFGHQASSNSSVDSQQGEFWNRGQT
                     GANGGRKFLDPCNLQLPLASIGYRRSSQLDFQNSPSWPMASTSDVPAFEFTAEGCAGA
                     HWLERPEVDDGTSEEENESDSSSSRTSNSSQTLSSCQAMEPCSSDEFFQALNHAEQTF
                     KKMENYLRHKQLCDVILVAGDRRIPAHRLVLSSVSDYFAAMFTNDVREARQEEIKMEG
                     VEPNSLWSLIQYAYTGRLELKEDNIECLLSTACLLQLSQVVEACCKFLMKQLHPSNCL
                     GIRSFADAQGCTDLHKVAHNYTMEHFMEVIRNQEFVLLPANEIAKLLASDDMNIPNEE
                     TILNALLTWVRHDLEQRRKDLSRLLAYIRLPLLAPQFLADMENNALFRDDIECQKLIM
                     EAMKYHLLPERRPMLQSPRTKPRKSTVGTLFAVGGMDSTKGATSIEKYDLRTNMWTPV
                     ANMNGRRLQFGVAVLDDKLYVVGGRDGLKTLNTVECYNPKTKTWSVMPPMSTHRHGLG
                     VAVLEGPMYAVGGHDGWSYLNTVERWDPQARQWNFVATMSTPRSTVGVAVLSGKLYAV
                     GGRDGSSCLKSVECFDPHTNKWTLCAQMSKRRGGVGVTTWNGLLYAIGGHDAPTSNLT
                     SRLSDCVERYDPKTDVWTAVASMSVSRDAVGVCLLGDKLYAVGGYDGQTYLNIVEAYD
                     PQTNEWTQVAPLCLGRAGACVVTVKI"
     exon            547..729
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            730..866
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1063
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1276
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1463
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1688
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1851
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..2064
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2236
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..3408
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3225..3230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="hexamer: ATTACA"
     polyA_site      3244
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="major polyA site"
     regulatory      3332..3337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="hexamer: TATAAA"
     regulatory      3349..3354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="hexamer: ACTAAA"
     polyA_site      3354
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="major polyA site"
     polyA_site      3372
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="major polyA site"
     regulatory      3386..3391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
                     /note="hexamer: AATATA"
     polyA_site      3408
                     /gene="Klhl5"
                     /gene_synonym="1300013C10Rik"
ORIGIN      
        1 attgggatga aagtaaatgg agatatatat ttgaacgatt tgttttcttt taaagctatt
       61 ttttgggtac agcagggaat atacttctct tgtcttggtt ggatacacaa atctgtgcgc
      121 cgtgtttttt gcccgttgcc tagacgatca ctcggtttct ctgaggatgt ctggttctcg
      181 caaagagttt gacgtgaaac agattttgaa aatcaggtgg aggtggtttg gccatcaagc
      241 atcgtctaac tcttccgttg acagccagca gggagaattt tggaaccgag gacagaccgg
      301 agcaaacggt gggagaaagt ttttagatcc gtgtaaccta caattgcctt tggcttccat
      361 tggttaccga aggtccagcc aactggattt ccagaattca ccttcttggc caatggcatc
      421 cacctcagat gtccctgcat ttgagtttac agcagaaggc tgtgctggtg cacactggct
      481 ggagagacca gaagtggacg atggcactag tgaagaggaa aatgaatctg attccagctc
      541 gagcaggact tccaatagta gtcagacatt gtcttcctgc caggctatgg agccatgctc
      601 atcagatgaa ttctttcaag cccttaacca tgcagagcaa acatttaaaa aaatggaaaa
      661 ctacttgagg cacaagcaac tgtgtgacgt cattttagtc gctggcgacc gcaggattcc
      721 agctcacaga ctggtgctgt cttccgtctc agattatttc gctgctatgt ttactaatga
      781 tgtcagggag gcgaggcaag aggaaatcaa aatggaaggt gtggagccga attccttgtg
      841 gtccttaatt cagtacgcgt acacaggccg acttgagctg aaggaagaca acatcgagtg
      901 cttgctgtcc acagcctgcc tgctccagct ctcccaggtg gtggaagcct gctgcaagtt
      961 cttgatgaag cagctccacc cgtccaactg cctggggatt cggtccttcg cggatgccca
     1021 aggctgcaca gatctgcata aagtggctca caattacacg atggaacatt tcatggaagt
     1081 aatcaggaac caggagtttg tgttactccc agccaatgag atcgccaagc tcttggccag
     1141 cgatgacatg aacatcccga acgaggagac catactgaac gcgcttctca cctgggtccg
     1201 gcacgacctg gagcagaggc ggaaggatct cagtagactt ttggcttaca ttaggctacc
     1261 tctccttgcg ccacagttcc tggcagacat ggaaaacaat gcacttttcc gggatgacat
     1321 tgaatgtcag aaactcatca tggaagcaat gaagtaccat ttattacctg agagacgacc
     1381 catgttgcag agtcctcgga caaagcctcg caagtcaact gttggaacgt tatttgcagt
     1441 tggagggatg gattcaacaa agggagcaac aagcattgag aagtatgacc tccgtacaaa
     1501 tatgtggact cctgtggcaa acatgaatgg gaggaggctc caattcggcg tcgcagtctt
     1561 agatgacaaa ctgtacgtgg tcggagggcg agacggactg aagactctga acaccgtaga
     1621 gtgctacaac cccaaaacaa aaacctggag tgtgatgcca cctatgtcca cacacaggca
     1681 cgggctcggc gtggctgtac tagaaggccc catgtatgca gtcggagggc acgacggctg
     1741 gagctacctg aatactgtgg agcgatggga ccctcaggcc cgccagtgga attttgttgc
     1801 cactatgtcc acccccagga gcacagtagg cgtggcggta ctaagtggaa aactctatgc
     1861 agttggtggt cgcgatggga gctcttgcct caagtcagta gaatgctttg accctcatac
     1921 caataaatgg acactgtgtg cccagatgtc aaaacgaaga ggcggagtag gagtcacaac
     1981 ctggaacggg ctgctgtatg ccattggggg acacgacgct cccacatcca atttgacctc
     2041 caggctctca gactgtgtgg agagatatga ccccaaaaca gacgtgtgga cagcagtggc
     2101 atccatgagc gttagcagag acgcggtggg cgtgtgtcta cttggtgaca agctgtatgc
     2161 tgtcgggggt tatgacggac agacctacct gaacatagtg gaggcttacg acccccagac
     2221 caacgaatgg acccaggttg ctccgctgtg cctaggaaga gctggagcct gtgttgtgac
     2281 tgtgaagata tgacatcatt ctttgtcctc cctctctaac acggacagtc ctcccatgga
     2341 ttcattctta gtgaatgtgt ggtgccacat tcctaggcac aaagtgcctc attccacagt
     2401 gaagatgtgg aagcgatgga ggagcggatg caccaggacg gatgcttctt tctcagtacg
     2461 ttcaagccac agccagagcg ctgggaacac gccctcttgt atacaatatg aagacaagtg
     2521 caccgcgcta actggattca cattgtctag acttgttcca gtacaagcat ccaagcttct
     2581 aaacggttat tttataagtt tgtcatacat gagcttccat actttttatc atttaatcaa
     2641 accgctattt tactgacata ctaaagacta tactagctag ctttttcagg tacaatgaca
     2701 ttatgtatgc tatagttgtg aattttatta ctaaaatggg tttaacccaa aaataaagaa
     2761 aaccacgtaa gacattaaaa ataagactga ccaaataaaa acttgactgg atctctgggt
     2821 cctacttaaa tagccattcc attacatcta gatccatcga ctgtgatagg atgtcactga
     2881 gattaaagct ggatgcttac attaagatat gcaagtttgt gtagagaaaa taaatgctat
     2941 atgctctcta atgttctccc acctaactag tatttaatta tgtggattta ttttctttta
     3001 tattacaaag gtttatttgg ttttgtcctt ttgatactga cacaattgtt tggctgttcc
     3061 tgggttctct ttgctgcctt gttccagacc agtggacaca gtgaggaacc cattgtgctt
     3121 ctttgtaatc aagaaactgg ggggtggggg acatgttagc tgaatatttg aaaaataagt
     3181 ttaaagcatt tgttactcaa aattttgtac atttgtaaac tctaattaca tatgtgaatg
     3241 ttaatagtgt ctgaattgtt tattgtttgt aaaatgccct gggcagcaat attgtatgaa
     3301 attcccttta aaagcgaaat cattacctta atataaaaat ggatacaaac taaagtccca
     3361 tccttcaaag tacaatgttc tggttaatat attcttattt tatattaaaa aaaaaaaaaa
     3421 aa
//
