LOCUS       NM_172781               3884 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus kelch-like 4 (Klhl4), transcript variant 1, mRNA.
ACCESSION   NM_172781
VERSION     NM_172781.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Chiu,H.S., Szucsik,J.C., Georgas,K.M., Jones,J.L., Rumballe,B.A.,
            Tang,D., Grimmond,S.M., Lewis,A.G., Aronow,B.J., Lessard,J.L. and
            Little,M.H.
  TITLE     Comparative gene expression analysis of genital tubercle
            development reveals a putative appendicular Wnt7 network for the
            epidermal differentiation
  JOURNAL   Dev Biol 344 (2), 1071-1087 (2010)
   PUBMED   20510229
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK081448.1, AK081937.1 and BX005189.9.
            
            On Mar 13, 2014 this sequence version replaced NM_172781.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK081937.1, BC057137.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-187               AK081448.1         2-188
            188-3453            AK081937.1         1-3266
            3454-3884           BX005189.9         108964-109394
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 49.47 cM"
     gene            1..3884
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="kelch-like 4"
                     /db_xref="GeneID:237010"
                     /db_xref="MGI:MGI:2442829"
     exon            1..513
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..2245
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kelch-like protein 4"
                     /codon_start=1
                     /product="kelch-like protein 4 isoform 1"
                     /protein_id="NP_766369.2"
                     /db_xref="CCDS:CCDS41108.2"
                     /db_xref="GeneID:237010"
                     /db_xref="MGI:MGI:2442829"
                     /translation="MSVSGKKEFDVKQILRLRWRWFSHPSQGSPNTGSCLQQEGYEHR
                     GTPVQGRLKNHSRDRNGLKKSNSPVHHDILTPVPGPAPTHQRVLQNWHQQNVIVHLQA
                     NEDTPKAVPEGNLLKEACEKRSRDLEMMGDDNIEDMRAGPCVEEPEQMTTSSNDHYRS
                     RSHADYTLCKMESYLKERKLCDVLLIAGPLKIPAHRLVLSAVSDYFAAMFTNDVLEAR
                     QEEVKIEGVDPNALNSLVQYAYTGVLQLREDTIENLLSAACLLQLTQVIDVCCNFLIK
                     QLHPSNCLGIRSFGDAQGCMELQNVAHKYAMEHFIDVIKNQEFLLLPANEISKLLCSD
                     DINVPDEETIFHALMQWVGHDAQARQRDLAKLLSYIRLPLLSPQLLADLENSSFFSGN
                     LECQKLLMEAMKYHLLPERRSMLQSPRTKPRKSTVGALYAVGGMDAAKGTTTIEKYDL
                     RTNSWIHIGTMSGRRLQFGVAVVDNKLYVVGGRDGLKTLNTVECFNPVTKTWVVMPPM
                     STHRHGLGVATLEGPMYAVGGHDGWSYLNTVERWDPDGRQWNYVASMSTPRSTVGVVA
                     LNNRLYAIGGRDGSSCLKSMEFFDPHTNKWSLCAPMSKRRGGVGVATHNGYLYVVGGH
                     DAPAPNHCSRLSDCVERYDPKGDSWSTVAPLSVPRDAVAVCPLGDKLYVVGGYDGHTY
                     LNTVESYDAQKDEWKEEVPVNIGRAGACVVVVKLQ"
     exon            514..678
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            679..815
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            816..1012
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1225
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1412
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1637
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1800
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..2013
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2185
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..3884
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3563..3568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3584
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="major polyA site"
ORIGIN      
        1 agttctacac aagaggcaga gagaaacaag agataacaaa ggctccattt cctttctgtt
       61 agagaaggct tttgtctttt ctcaggcaac aatgtcagtg tccggcaaga aagagtttga
      121 tgtgaaacag atcctgaggc tgcgctggag atggtttagt catccttctc aaggatcgcc
      181 gaacactgga agctgccttc agcaggaagg atatgagcat agagggaccc cggtgcaggg
      241 caggttgaag aaccactcac gggacagaaa cggactgaag aaaagcaata gtcctgtcca
      301 ccacgatata ctgacgccag taccaggacc agcccctacc catcaaagag tccttcagaa
      361 ttggcaccaa caaaacgtga tagtacatct tcaagcaaat gaagacactc ctaaagccgt
      421 tccagaagga aatttgctca aagaggcttg tgagaagcgc tcacgagatt tggagatgat
      481 gggtgatgac aatatagaag atatgagagc aggaccatgt gttgaggaac ctgaacaaat
      541 gaccaccagt tctaatgatc actaccgttc tcgaagccat gcagattaca ctctctgtaa
      601 aatggaaagc tatttgaaag agagaaaact gtgtgatgtg ctacttattg ctggacctct
      661 taaaatccca gctcaccgat tggttctcag tgctgtgtct gattattttg ctgcaatgtt
      721 tactaatgat gtgcttgaag ccagacaaga agaggtcaaa atagaaggag tagatccaaa
      781 tgcacttaat tctttagtgc agtatgctta tacaggtgtg ctgcaactga gagaagacac
      841 cattgaaaat ttgctgtcag cagcctgtct cttacagctg actcaggtca ttgatgtttg
      901 ctgcaatttc ctcataaagc aacttcaccc ttcaaactgc ctagggattc gctcatttgg
      961 agatgcacag ggctgtatgg agcttcagaa tgtggcacat aagtatgcca tggaacactt
     1021 cattgatgta ataaagaacc aagaattcct gctgcttcca gctaatgaaa tatcaaaact
     1081 tctgtgtagt gatgacatta atgtgcctga tgaagaaaca attttccatg ctcttatgca
     1141 gtgggtaggt catgatgctc aggctaggca acgagatctg gcaaagctgc tttcttacat
     1201 tagactgcca ttactctcac cacagttgct tgcagatctg gaaaacagtt cattcttcag
     1261 tggtaatctg gaatgtcaga agctactaat ggaagctatg aagtatcatc tcttgcctga
     1321 gagaaggtcc atgttgcaaa gccctcgtac aaagcctaga aaatcaactg tgggggcact
     1381 ttatgctgtg ggaggtatgg atgctgctaa aggcaccact acaattgaga agtatgacct
     1441 ccgaaccaac agttggatcc acattggcac tatgagtggt cgtagacttc aatttggagt
     1501 ggcagttgtt gataataaac tttacgttgt gggaggaaga gatggcttaa aaactttgaa
     1561 tactgtggaa tgtttcaatc cggttactaa aacctgggtt gtaatgcctc ctatgtccac
     1621 acataggcac gggctaggcg tagccacact tgaaggacca atgtatgcgg ttggaggtca
     1681 cgatggatgg agctatctaa acactgtaga aagatgggac cctgatggcc gtcagtggaa
     1741 ttatgtggcc agcatgtcaa ctccaagaag cacagttgga gttgttgcat taaacaatag
     1801 gctgtatgct attggtggac gtgatggaag ttcctgtctt aagtccatgg aattctttga
     1861 cccgcatact aacaagtgga gtctgtgtgc cccgatgtcc aaaagacgtg gaggtgtagg
     1921 agtagcaaca cacaatggat acttgtatgt tgtagggggt catgatgccc ctgctcctaa
     1981 tcattgctcc aggctttctg actgcgtgga aagatacgac ccaaaaggcg attcatggtc
     2041 aactgtggca cctttgagtg ttcctcgaga tgctgttgca gtgtgccccc ttggagacaa
     2101 actctacgtg gttggcggat atgatggtca tacttacttg aataccgttg agtcgtatga
     2161 tgctcagaaa gacgaatgga aagaggaagt ccctgtgaac attggaagag ctggagcatg
     2221 tgttgttgtt gtgaagctgc agtgaaagta tcatttgcta atgcaatgaa atggatttca
     2281 agaatgaaga aaaacatact cttttatgaa gttcataagc aaactaaatg ctgttgtgtc
     2341 attctcacat atatttgaaa ccgttaacat tcataaggct gaaagcaaaa cttaaatatg
     2401 aattacatgt tagtattgtg tatatgtgct cagtttataa tctaatgaga aatccaatag
     2461 gcaattattt aggctcatta ccataaaatg ttaaaatacc tggtggatat ttggtagtgg
     2521 gcaatatttg aaggtgatga atacattaac agtttttatg taagaatctg agaagtaaaa
     2581 aaaagaaact gttaaaggta ctctactgtc tagaagttta atacaaaatg acagaaactt
     2641 taagcattcc atctggtttc caaacaatac cttacattgc tgataatacc tcacatttgt
     2701 caagaagaaa ggtttttctg tgtcaaaact gaaagagatc atttactgat ataatttgct
     2761 atactaatcc caccacttca aacttttcac aagcctatta tttttagtta actatctgcc
     2821 aaaatacatg tatagatttt ctgtttcttg aagtagtgca ttgattgatt catttgcagt
     2881 aaaatatcat aatgtcacta tataatcctt attcggaaac agtgaatcag tgagtattat
     2941 attgttacaa actgtgacct aagcttcaga atgtgagtgc ctcatttgta tagaaattaa
     3001 ttttctcttc aaacatttaa tgtgttccat gtaaacacct tccattaagt tttaaaaagt
     3061 tgatttaaac aattctaaag gagattataa ttgagaaaat gatccacaat gctgacactt
     3121 agatacattg aaatggttaa tatacactgt tacttctata atttctattt acctatcaat
     3181 agctgtgttt cttttaattt cataataatt tttatcccgt aggagatggt aaggataaat
     3241 gtactacaac tacacatttg aaggtaaaaa aaaaaaaata gtactgagtc acattttagc
     3301 ttttaactat tattttcttt tatcctccct aatgacaaaa caatatggtg aaacattgac
     3361 aactcacatt tatgctgtta acttttcaag taaatgttcc actttagaat ttctatatct
     3421 tcttttaaat gacctttgta ttgtagatta tccaaaaaaa tccagagaga tgcttataat
     3481 atgtattagt gctacctatt aattttttaa aatcacatca taagttgcac aaaaatgttt
     3541 gtactatgac cagctgaatt taaataaaat cttttctctc tggctataga gactgtgatt
     3601 ttagaatatg ctgaatgtgt gctgaatgat ccgtccctgc tgctgtgcca caatagtgaa
     3661 catattgaaa tgctaatata tagtttactc tttttttcat aatttgtctg taattctcaa
     3721 cagagatcta cagatttttc catgcttaga ggtacgacag aatgacagag tggcattaag
     3781 aggctataag acacgtataa gaccttgcta gagtttttaa tatttgttta acgtgtatta
     3841 attatacgta ataatgagtt gccttgtgac atttaataca taag
//
