LOCUS       NM_001290477            3866 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus kelch-like 4 (Klhl4), transcript variant 2, mRNA.
ACCESSION   NM_001290477 XM_006528385
VERSION     NM_001290477.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Chiu,H.S., Szucsik,J.C., Georgas,K.M., Jones,J.L., Rumballe,B.A.,
            Tang,D., Grimmond,S.M., Lewis,A.G., Aronow,B.J., Lessard,J.L. and
            Little,M.H.
  TITLE     Comparative gene expression analysis of genital tubercle
            development reveals a putative appendicular Wnt7 network for the
            epidermal differentiation
  JOURNAL   Dev Biol 344 (2), 1071-1087 (2010)
   PUBMED   20510229
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK085099.1, AK081937.1 and BX005189.9.
            
            On Mar 13, 2014 this sequence version replaced XM_006528385.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK081448.1, AK085099.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2558              AK085099.1         2-2559
            2559-3435           AK081937.1         2390-3266
            3436-3866           BX005189.9         108964-109394
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 49.47 cM"
     gene            1..3866
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="kelch-like 4"
                     /db_xref="GeneID:237010"
                     /db_xref="MGI:MGI:2442829"
     exon            1..513
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..2227
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="isoform 2 is encoded by transcript variant 2;
                     kelch-like protein 4"
                     /codon_start=1
                     /product="kelch-like protein 4 isoform 2"
                     /protein_id="NP_001277406.1"
                     /db_xref="CCDS:CCDS72423.1"
                     /db_xref="GeneID:237010"
                     /db_xref="MGI:MGI:2442829"
                     /translation="MSVSGKKEFDVKQILRLRWRWFSHPSQGSPNTGSCLQQEGYEHR
                     GTPVQGRLKNHSRDRNGLKKSNSPVHHDILTPVPGPAPTHQRVLQNWHQQNVIVHLQA
                     NEDTPKAVPEGNLLKEACEKRSRDLEMMGDDNIEDMRAGPCVEEPEQMTTSSNDHYRS
                     RSHADYTLCKMESYLKERKLCDVLLIAGPLKIPAHRLVLSAVSDYFAAMFTNDVLEAR
                     QEEVKIEGVDPNALNSLVQYAYTGVLQLREDTIENLLSAACLLQLTQVIDVCCNFLIK
                     QLHPSNCLGIRSFGDAQGCMELQNVAHKYAMEHFIDVIKNQEFLLLPANEISKLLCSD
                     DINVPDEETIFHALMQWVGHDAQARQRDLAKLLSYIRLPLLSPQLLADLENSSFFSGN
                     LECQKLLMEAMKYHLLPERRSMLQSPRTKPRKSTVGALYAVGGTTTIEKYDLRTNSWI
                     HIGTMSGRRLQFGVAVVDNKLYVVGGRDGLKTLNTVECFNPVTKTWVVMPPMSTHRHG
                     LGVATLEGPMYAVGGHDGWSYLNTVERWDPDGRQWNYVASMSTPRSTVGVVALNNRLY
                     AIGGRDGSSCLKSMEFFDPHTNKWSLCAPMSKRRGGVGVATHNGYLYVVGGHDAPAPN
                     HCSRLSDCVERYDPKGDSWSTVAPLSVPRDAVAVCPLGDKLYVVGGYDGHTYLNTVES
                     YDAQKDEWKEEVPVNIGRAGACVVVVKLQ"
     exon            514..678
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            679..815
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            816..1012
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1225
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1394
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1619
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1782
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1995
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2167
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..3866
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3545..3550
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3566
                     /gene="Klhl4"
                     /gene_synonym="B130014A11; C130018J01Rik"
                     /note="major polyA site"
ORIGIN      
        1 agttctacac aagaggcaga gagaaacaag agataacaaa ggctccattt cctttctgtt
       61 agagaaggct tttgtctttt ctcaggcaac aatgtcagtg tccggcaaga aagagtttga
      121 tgtgaaacag atcctgaggc tgcgctggag atggtttagt catccttctc aaggatcgcc
      181 gaacactgga agctgccttc agcaggaagg atatgagcat agagggaccc cggtgcaggg
      241 caggttgaag aaccactcac gggacagaaa cggactgaag aaaagcaata gtcctgtcca
      301 ccacgatata ctgacgccag taccaggacc agcccctacc catcaaagag tccttcagaa
      361 ttggcaccaa caaaacgtga tagtacatct tcaagcaaat gaagacactc ctaaagccgt
      421 tccagaagga aatttgctca aagaggcttg tgagaagcgc tcacgagatt tggagatgat
      481 gggtgatgac aatatagaag atatgagagc aggaccatgt gttgaggaac ctgaacaaat
      541 gaccaccagt tctaatgatc actaccgttc tcgaagccat gcagattaca ctctctgtaa
      601 aatggaaagc tatttgaaag agagaaaact gtgtgatgtg ctacttattg ctggacctct
      661 taaaatccca gctcaccgat tggttctcag tgctgtgtct gattattttg ctgcaatgtt
      721 tactaatgat gtgcttgaag ccagacaaga agaggtcaaa atagaaggag tagatccaaa
      781 tgcacttaat tctttagtgc agtatgctta tacaggtgtg ctgcaactga gagaagacac
      841 cattgaaaat ttgctgtcag cagcctgtct cttacagctg actcaggtca ttgatgtttg
      901 ctgcaatttc ctcataaagc aacttcaccc ttcaaactgc ctagggattc gctcatttgg
      961 agatgcacag ggctgtatgg agcttcagaa tgtggcacat aagtatgcca tggaacactt
     1021 cattgatgta ataaagaacc aagaattcct gctgcttcca gctaatgaaa tatcaaaact
     1081 tctgtgtagt gatgacatta atgtgcctga tgaagaaaca attttccatg ctcttatgca
     1141 gtgggtaggt catgatgctc aggctaggca acgagatctg gcaaagctgc tttcttacat
     1201 tagactgcca ttactctcac cacagttgct tgcagatctg gaaaacagtt cattcttcag
     1261 tggtaatctg gaatgtcaga agctactaat ggaagctatg aagtatcatc tcttgcctga
     1321 gagaaggtcc atgttgcaaa gccctcgtac aaagcctaga aaatcaactg tgggggcact
     1381 ttatgctgtg ggaggcacca ctacaattga gaagtatgac ctccgaacca acagttggat
     1441 ccacattggc actatgagtg gtcgtagact tcaatttgga gtggcagttg ttgataataa
     1501 actttacgtt gtgggaggaa gagatggctt aaaaactttg aatactgtgg aatgtttcaa
     1561 tccggttact aaaacctggg ttgtaatgcc tcctatgtcc acacataggc acgggctagg
     1621 cgtagccaca cttgaaggac caatgtatgc ggttggaggt cacgatggat ggagctatct
     1681 aaacactgta gaaagatggg accctgatgg ccgtcagtgg aattatgtgg ccagcatgtc
     1741 aactccaaga agcacagttg gagttgttgc attaaacaat aggctgtatg ctattggtgg
     1801 acgtgatgga agttcctgtc ttaagtccat ggaattcttt gacccgcata ctaacaagtg
     1861 gagtctgtgt gccccgatgt ccaaaagacg tggaggtgta ggagtagcaa cacacaatgg
     1921 atacttgtat gttgtagggg gtcatgatgc ccctgctcct aatcattgct ccaggctttc
     1981 tgactgcgtg gaaagatacg acccaaaagg cgattcatgg tcaactgtgg cacctttgag
     2041 tgttcctcga gatgctgttg cagtgtgccc ccttggagac aaactctacg tggttggcgg
     2101 atatgatggt catacttact tgaataccgt tgagtcgtat gatgctcaga aagacgaatg
     2161 gaaagaggaa gtccctgtga acattggaag agctggagca tgtgttgttg ttgtgaagct
     2221 gcagtgaaag tatcatttgc taatgcaatg aaatggattt caagaatgaa gaaaaacata
     2281 ctcttttatg aagttcataa gcaaactaaa tgctgttgtg tcattctcac atatatttga
     2341 aaccgttaac attcataagg ctgaaagcaa aacttaaata tgaattacat gttagtattg
     2401 tgtatatgtg ctcagtttat aatctaatga gaaatccaat aggcaattat ttaggctcat
     2461 taccataaaa tgttaaaata cctggtggat atttggtagt gggcaatatt tgaaggtgat
     2521 gaatacatta acagttttta tgtaagaatc tgagaagtaa aaaaaagaaa ctgttaaagg
     2581 tactctactg tctagaagtt taatacaaaa tgacagaaac tttaagcatt ccatctggtt
     2641 tccaaacaat accttacatt gctgataata cctcacattt gtcaagaaga aaggtttttc
     2701 tgtgtcaaaa ctgaaagaga tcatttactg atataatttg ctatactaat cccaccactt
     2761 caaacttttc acaagcctat tatttttagt taactatctg ccaaaataca tgtatagatt
     2821 ttctgtttct tgaagtagtg cattgattga ttcatttgca gtaaaatatc ataatgtcac
     2881 tatataatcc ttattcggaa acagtgaatc agtgagtatt atattgttac aaactgtgac
     2941 ctaagcttca gaatgtgagt gcctcatttg tatagaaatt aattttctct tcaaacattt
     3001 aatgtgttcc atgtaaacac cttccattaa gttttaaaaa gttgatttaa acaattctaa
     3061 aggagattat aattgagaaa atgatccaca atgctgacac ttagatacat tgaaatggtt
     3121 aatatacact gttacttcta taatttctat ttacctatca atagctgtgt ttcttttaat
     3181 ttcataataa tttttatccc gtaggagatg gtaaggataa atgtactaca actacacatt
     3241 tgaaggtaaa aaaaaaaaaa tagtactgag tcacatttta gcttttaact attattttct
     3301 tttatcctcc ctaatgacaa aacaatatgg tgaaacattg acaactcaca tttatgctgt
     3361 taacttttca agtaaatgtt ccactttaga atttctatat cttcttttaa atgacctttg
     3421 tattgtagat tatccaaaaa aatccagaga gatgcttata atatgtatta gtgctaccta
     3481 ttaatttttt aaaatcacat cataagttgc acaaaaatgt ttgtactatg accagctgaa
     3541 tttaaataaa atcttttctc tctggctata gagactgtga ttttagaata tgctgaatgt
     3601 gtgctgaatg atccgtccct gctgctgtgc cacaatagtg aacatattga aatgctaata
     3661 tatagtttac tctttttttc ataatttgtc tgtaattctc aacagagatc tacagatttt
     3721 tccatgctta gaggtacgac agaatgacag agtggcatta agaggctata agacacgtat
     3781 aagaccttgc tagagttttt aatatttgtt taacgtgtat taattatacg taataatgag
     3841 ttgccttgtg acatttaata cataag
//
