LOCUS       NM_001163020            7152 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus kelch-like 32 (Klhl32), transcript variant 1, mRNA.
ACCESSION   NM_001163020
VERSION     NM_001163020.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
REFERENCE   3  (bases 1 to 7152)
  AUTHORS   Kudo,T., Kaneko,M., Iwasaki,H., Togayachi,A., Nishihara,S., Abe,K.
            and Narimatsu,H.
  TITLE     Normal embryonic and germ cell development in mice lacking alpha
            1,3-fucosyltransferase IX (Fut9) which show disappearance of
            stage-specific embryonic antigen 1
  JOURNAL   Mol Cell Biol 24 (10), 4221-4228 (2004)
   PUBMED   15121843
REFERENCE   4  (bases 1 to 7152)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   5  (bases 1 to 7152)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   6  (bases 1 to 7152)
  AUTHORS   Phelan,S.A., Johnson,K.A., Beier,D.R. and Paigen,B.
  TITLE     Characterization of the murine gene encoding Aop2 (antioxidant
            protein 2) and identification of two highly related genes
  JOURNAL   Genomics 54 (1), 132-139 (1998)
   PUBMED   9806838
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY281890.1, AL772223.7, CJ093672.1, CA556185.2, AK134944.1,
            AL671913.11 and BF456570.1.
            
            On Sep 21, 2017 this sequence version replaced NM_001163020.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.174836.1,
                                           SRR7652917.548759.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164136, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BY281890.1         1-26
            27-27               AL772223.7         72085-72085         c
            28-38               BY281890.1         28-38
            39-447              CJ093672.1         1-409
            448-979             CA556185.2         2-533
            980-1305            AK134944.1         815-1140
            1306-1306           AL671913.11        76107-76107
            1307-2424           AK134944.1         1142-2259
            2425-6847           AL671913.11        108872-113294
            6848-7152           BF456570.1         1-305               c
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 10.42 cM"
     gene            1..7152
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /note="kelch-like 32"
                     /db_xref="GeneID:212390"
                     /db_xref="MGI:MGI:3612790"
     exon            1..387
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            388..472
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     CDS             450..2312
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /note="isoform 1 is encoded by transcript variant 1; BTB
                     and kelch domain containing 5"
                     /codon_start=1
                     /product="kelch-like protein 32 isoform 1"
                     /protein_id="NP_001156492.1"
                     /db_xref="CCDS:CCDS51131.1"
                     /db_xref="GeneID:212390"
                     /db_xref="MGI:MGI:3612790"
                     /translation="MPSEHCLSIQEMLTGQRLCHSETHNDTVLAALNQQRSDGILCDV
                     TLIAEEQKFHAHKAVLAACSDYFRAMFSLCMVESGADEVNLRGVTSLGLKQALEFAYT
                     GQILLEPGVIQDVLAAGSHLQLLELLNLCSHYLIQELNSFNYLDLYRLADLFHLTLLE
                     KAVIDFLVKHLSELLKSRPEDVLTLPYCLLQEVLKSDRLTSLSEEQIWQLAVRWLEHN
                     CHYQHLDELLQYIRFGLMDVDTLHTVALSHPLVQASETATALVNEALEYHQSIYAQPV
                     WQTRRTKPRFQSDTLYIIGGKKREVCKVKELRYFNPVDQENALIAAIANWSELAPMPV
                     GRSHHCVAVMGDFLFVAGGEVEHASGRTCAVRTACRYDPRSNSWAEIAPMKNCREHFV
                     LGAMDEYLYAVGGRNELCQVLPTVERYCPKKNKWTFVQSFDRSLSCHAGYVADGLLWI
                     SGGVTNTAQYQNRLMVYEPNQNKWISRSPMLQRRVYHSMAAVQRKLYVLGGNDLDYNN
                     DRILVRHIDSYNIDTDQWTRCNFNLLTGQNESGVAVHNGRIYLVGGYSIWTNEPLACI
                     QVLDVSREGKEEVFYGPTLPFASNGIAACFLPAPYFTCPNLQTLQVPHHRIGTV"
     exon            473..653
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            654..761
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            762..860
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1076
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1803
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1862
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..2055
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2150
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..7138
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3704..3709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /note="hexamer: AATAAA"
     polyA_site      3731
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /note="major polyA site"
     regulatory      5249..5254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
                     /note="hexamer: AATAAA"
     polyA_site      5269
                     /gene="Klhl32"
                     /gene_synonym="6430524H05Rik; D4Ertd389e; Gm1356;
                     mKIAA1900"
ORIGIN      
        1 gaacgcggcg gggcgggagc gcgcaccggc aggagggcgg agcggctgtg aggggaggct
       61 ggagaccggc tgctgggagg gtagagcggg ccgggtcgca cgcgccacct gcggggctgg
      121 ctggctttct cttcctgccg acacatcccg gtccctctcc gaagcccctg gagttcggga
      181 gcgcgcgggg tagcgcgcac accgactcgc atccacacac acgccagagc gcgcgatcat
      241 cactgctgca gcccgggcag ggcagacgga gctgctgcag tttccccagg gtcgagagga
      301 aagcagagct gtcttgcctg gcagagagcc tggtgcccgc tggagcccct ggggtatcaa
      361 gcccagcaga ctgctcccct ccatcggctg gaatgcttgc ggattcttct gaaaccttct
      421 aagcctgact ctgacgaccc agcttgagga tgccgtctga acactgcctc agtattcaag
      481 aaatgctgac gggccagagg ctgtgccact cggaaactca caatgacacg gtcctggcgg
      541 cgctgaatca gcaaagaagt gatggcattc tctgcgacgt gaccctgatc gccgaggaac
      601 agaaattcca cgctcacaag gcggtcctag cagcctgcag tgactacttc cgggcaatgt
      661 tcagtctttg tatggtggaa agtggagccg atgaagtaaa tttacgtggt gtgaccagcc
      721 tgggcttaaa gcaggctctg gagttcgcat atacaggaca gattttgttg gagccaggag
      781 tgatccagga tgtgttagca gctggcagtc acctacagct gttggaactt ctcaatctgt
      841 gctcccacta tctcatccag gaactaaata gctttaatta cttggatctg tacagacttg
      901 ctgacctctt tcacctcact ttgttggaga aggcagtgat tgacttccta gtgaagcatc
      961 tctcggagct cctgaagagc cgcccagagg atgtcctcac gctgccctac tgcctgcttc
     1021 aggaggtgct gaagagtgac cgcctgacgt ccctgagtga ggagcagatc tggcagctag
     1081 ctgtgaggtg gctggaacac aattgccatt accagcatct ggacgagctc ctgcagtaca
     1141 tccgctttgg cctaatggat gtggatacac tccacacggt tgccctatcc caccccctgg
     1201 tgcaggcaag tgagacggca actgcccttg tcaacgaggc cctggaatac caccaaagca
     1261 tctatgcaca gcctgtgtgg cagacccgcc ggacaaaacc gcgattccag tccgatactc
     1321 tctatattat tggtgggaag aagcgtgagg tctgcaaagt caaggaactt cgttacttca
     1381 atcctgtgga ccaggagaat gccctcatag ccgccattgc caactggagt gagctggctc
     1441 ccatgccggt gggaaggagc catcactgtg tggcagtcat gggggacttc ctgtttgtag
     1501 caggaggaga agtggaacat gccagtggcc ggacatgtgc cgtgaggaca gcctgtcgct
     1561 atgacccccg cagtaattcc tgggcagaga tagcacccat gaaaaactgc cgggaacatt
     1621 ttgtgctggg tgccatggat gaatacctct atgcagtagg gggcagaaat gaactgtgcc
     1681 aagttctacc tacagttgaa cgatactgcc ccaagaagaa caaatggact tttgtgcagt
     1741 cctttgacag atccctctcg tgtcacgctg gatatgtggc tgatggtctt ctttggatat
     1801 caggtggggt gacaaacacc gcacagtatc agaacagact gatggtatat gaacctaacc
     1861 agaataagtg gataagtcgc agccccatgc tacagagaag agtctaccac tccatggctg
     1921 ctgtccaaag gaagctttat gtgcttggag gcaatgacct ggattacaat aatgaccgga
     1981 ttctcgtgcg tcatatagat tcttacaaca ttgacactga tcagtggacg cgctgtaatt
     2041 tcaacttgtt gactggccag aatgaatctg gagtcgctgt ccataatggg agaatatatt
     2101 tagttggtgg atattcaatt tggacaaatg agcctctggc ttgtatccag gtattggatg
     2161 tgagcagaga aggcaaagag gaagtgttct acggacctac actgcccttt gcttccaacg
     2221 gcatagcggc gtgcttcctt ccggctccgt atttcacatg ccccaacctg cagactctgc
     2281 aagtgcctca ccacaggatc ggcactgtct gataagccag catccacagg agaccgaatg
     2341 agaccttgac atctggacac agggcatgga catcctcagt gcttccctat cgtgtcttac
     2401 aggtctaaaa ttgtgctaaa tactagcaaa aaaaaaaaaa aagaataatt ttctaaaaca
     2461 tattatcgag aattttgtca cttctttcag tgtgtgttaa aacatacaat atgtgtgatt
     2521 tttattgtgg tgcaccttag tgccatccta cggcacgtga tagttttgtg gctctttaaa
     2581 acattctttg tacccctgtc ctagttagtg ctgtctggga tcatgtgaca ctgtcagtaa
     2641 ggaaccttac acagcaggca tcagtttcct cataatcttc ggtacttagg ccttttcgtt
     2701 actcaagcat cagcgtgtct tttcaagctt accattctgc agcattcctc agcagaagtt
     2761 cagcgggatg acatcagata cactttaagt gccacatttc attcaaggtg atcggagtaa
     2821 aggacttgga taagttagat ttccaacctt ggttactctg accttgtttc agaactttat
     2881 agggctaggc ttctcaccct tggtgataat tgctcatttc cacatttact aaaaagaatg
     2941 ccatgaggat ttggcaaatg ctcagaactt tgcctaaggt taaaaaaaaa tgttacttac
     3001 attctaaaat gtgttgctat ctttagagtc ctgtaaccaa accattttat ttccaagttt
     3061 ataaatttcg aggagtgttg agacggtcct gtgggagtct gagagcattg ggaaggcgct
     3121 acacctatcc tgagcaggaa tgccagagat cccgaaactt ggacaaattc ttgcctcaaa
     3181 gaattctctt taaatgtctc atctacagat taaaaacatt acctgtacta accatggcac
     3241 cacggaaggc gttgacataa gtctgaaccg agagcctgca gccagcagtt ctaatagacg
     3301 ttttccaagt tgataaaggc aatgtcaatc ttgcctactt ggagggttct ccaccactgc
     3361 caatacattt ttttcttatt actacccctt ttaataatga ataattcttt tttagtctag
     3421 agttttgagt ttgctttatc tctacctaaa aataaatcat cttaacaatc acatttgttt
     3481 ttttctaatc ttgccacgac tggaggtcat ttctattaaa ggaaaagctg ctttttctca
     3541 gctgcttaag tcagttgctt atgtcataga aattgtctgt tttactcatg caaccactta
     3601 ttttgtatca tcataagcca acgaaaaact agttttcttc ttgtttaggg tggtcttcag
     3661 ttggctatgt agctaaactg gcctccaact tatgaccctc cccaataaaa caaccttttt
     3721 atcttaacaa atttactaag tttacgcatt gctgtatgat accgaaagca ctgttctaca
     3781 catataaatg ctctctggtt aattgggtta aagaacagga agtgatagaa aggataagga
     3841 agaaaggtta aaaactagga cttcagacag accaggacca tttggacagg cgatgtaggt
     3901 aagtggcgag aacggtttaa aagcagccca tgagccttca gagatgaaca taatcaatcc
     3961 gtcaggacag tgccttcctg ccttcctgat ctgcttcagc acagatctaa ataagaagtg
     4021 cttcacacgt actgaacttt ctacttgata catggggttt tctctctagc agcttttcgt
     4081 tgtaaaatca ttaacttgta tttatgttct cgggtcctct acccagtgag tgtaaggaaa
     4141 tgtcttaaaa ataagcttgc ctgacagata atggacctag actaacccta gactgctgct
     4201 gtatccgttt ctaaccattc actaaagtca gcgtcagtta ccaatttctc cttgatggtc
     4261 ttcatggggt actttttgca gtttggacag attcttcaca tctgtaatca aagagcagcc
     4321 tcagaagtgt cacactcctt taccaaaggg aaggaaaggt tgtacgattg gatcgctttc
     4381 tcaaactcat ctgcagtgaa aaagtctgtg gaatcgtatt tggtgtgtat aagcaagtcc
     4441 tacctgaact actcagctat atagctatta tttaagtgta gtatcctaca gtgatttaat
     4501 tttcattttc agtctttaga attaaaggct gtattttact ttccatttaa gactttgaag
     4561 tatttaaaca tcaagtaaag attttactta taaataaaag aaaagtttca atgattctgc
     4621 tgtgagagga aaagctagca aagacacaga cagcttctac ccacgatgca aggtgcagta
     4681 aaaccccata tctctaaaaa taaaatgcca agtaaattac aactgtggca cttcctgtac
     4741 cttcctcttg ttttcctcat acctaatgtg cataagtagt gtcccttcag ctattgcaaa
     4801 agttgaaacc ttcagttggt ttgaacccaa agtataaatg caataagaag aagacagata
     4861 ctgtttcact aagtaacatg gctcatcttt attttcaatc tatattcatg ggcacaatgt
     4921 atgtgaaagg agtacattac attttatata tctatctcat ctttattata tgcaaactac
     4981 ctataaatta catgagatat ctataatgta ataaactgta gttatcagca caacaaagtt
     5041 gtaagtaagg actaggggtg cagctcagtt ggaagagctg tcttgcgtgc agggtgcctg
     5101 gatttccatc acaagcagtg cattaaactg gctatagcag ctcatgtttg caatcccaat
     5161 atgcttgaga agtcttggct accaagagag ttcaaagaag ccaacctgaa aacagattct
     5221 gactctgtct ttcaaaaatg aatacaataa taaaattgat aaagtataat catgtggtat
     5281 tgaactgggt aacctcagca tcgcagatga tcacagtgaa aacacaaatc ctgtgtctaa
     5341 tcaaatatag gaaagaatag gttattttgt tagaaatcta gactgattgt ttcaaccaaa
     5401 tcaagcacat tgtttatact gctgttttac actcttgctc gaccaggtaa tatatttaac
     5461 tctaggaagg gtaggcattt agttttcctt agtgcagaaa agatatagaa cttagagaga
     5521 gatgagttcc ttaagagttt cccaaaatgc agaaacatat cagtttttat ataaaatctc
     5581 aactgccaaa gattctataa gaactgaaca tggttactgt gatggtacat gcctgacact
     5641 ccatcattcc agcacttgga gggagggagg ctgaggcagg aagattttat ggcctaggat
     5701 acctaggaga caaagataac caacaagaga ggaaaaaaat aaaagacatt ttgtgccaat
     5761 acatagaaat aagcccatat attctttaag aaccaaatgt cttcaaaaca agaaaagccc
     5821 agggagatga gaaaggtgct tatcaccaag cctgatagcc tgcattcgat cccgggatcc
     5881 atgtggtaca agaagagagt tagaattgac tcttccaagc tttcctttga cctacacaca
     5941 cacacacaca cacacacaca cacacacaca cacacgagag agagagagag agagagagag
     6001 agagagagag agagagagag aaagagagag aggtatatat gcccacacat ataatagttt
     6061 aatcttttgt ttaaaggagt gaaagcactc ttggaacata cagctataat cctgccattg
     6121 gagtgcctaa gagaaatttg agttggagtc tggcctgagc tatggtgagc cacaaaccaa
     6181 cagccagccc ctgtgtggac aaaacaaagt caccaagaaa acaaagcact aaacaaaacc
     6241 ccaccaaact tgtgaaaagg tctggtagaa gctacaggat aataaaaatt gagtaatctg
     6301 agacatacca atactatttt ctaaaatgct tagaaaacaa gtcaaatgca gtcatctctc
     6361 acttaatggc tgggatgtag tctgagaaat gcatccttag gggaaatcac tgtccttaca
     6421 caataggtag tcgtcagtca agacttgtgg cctctgcaga tttctgagtt cgaggccagc
     6481 ctggtctaca aagtgagttc caggacagcc agggctatac agagaaaccc tgtctcgaaa
     6541 aaccaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaacaacc aaaaaagact tgtggcctct
     6601 ggatgctatc ctgatttgtg gtgctggtgt aacaaggcat actgttttac agttaaattt
     6661 ttttttgagt acttttctac attaggtatg taatctaaaa ctgtgagggg aaataaatac
     6721 acaaagcagc aagtcatcac tcatgaagga ccacgtactg tgcacagatg gatgtgttct
     6781 gtatagctct gtgtccagca gggcagtggg cttgcctgtc cttgcattag cccatgtgtt
     6841 gcctatccta tactgtgatg ttacaatggc tatgtaccta ggtgttagga acctcagagc
     6901 tctgccatca tctcatgatc ttacctgggt atgtgtgtcc catctcttaa acatcatgca
     6961 ccatatggtt ttacttaagg gaaaaaaaaa gataggtaaa gatatagatg tttaaaatta
     7021 tgtaatcctt ctagcacaaa tgaactcaat actgattttt gcagatgatc ttccaaattt
     7081 ttcttaccca tggaaaccat ctaattagat gataattaaa ttgaatttta aatcaaaaaa
     7141 aaaaaaaaaa aa
//
