LOCUS       NM_001359068            2606 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus kelch-like 22 (Klhl22), transcript variant 2, mRNA.
ACCESSION   NM_001359068 XM_006522040
VERSION     NM_001359068.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Kong,X., Shu,X., Wang,J., Liu,D., Ni,Y., Zhao,W., Wang,L., Gao,Z.,
            Chen,J., Yang,B., Guo,X. and Wang,Z.
  TITLE     Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3 ubiquitin
            ligase cascade in brain development
  JOURNAL   Development 149 (24) (2022)
   PUBMED   36440598
  REMARK    GeneRIF: Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3
            ubiquitin ligase cascade in brain development.
REFERENCE   3  (bases 1 to 2606)
  AUTHORS   Zhou,X.A., Zhou,J., Zhao,L., Yu,G., Zhan,J., Shi,C., Yuan,R.,
            Wang,Y., Chen,C., Zhang,W., Xu,D., Ye,Y., Wang,W., Shen,Z., Wang,W.
            and Wang,J.
  TITLE     KLHL22 maintains PD-1 homeostasis and prevents excessive T cell
            suppression
  JOURNAL   Proc Natl Acad Sci U S A 117 (45), 28239-28250 (2020)
   PUBMED   33109719
REFERENCE   4  (bases 1 to 2606)
  AUTHORS   Chen,J., Ou,Y., Yang,Y., Li,W., Xu,Y., Xie,Y. and Liu,Y.
  TITLE     KLHL22 activates amino-acid-dependent mTORC1 signalling to promote
            tumorigenesis and ageing
  JOURNAL   Nature 557 (7706), 585-589 (2018)
   PUBMED   29769719
REFERENCE   5  (bases 1 to 2606)
  AUTHORS   Lund,J., Chen,F., Hua,A., Roe,B., Budarf,M., Emanuel,B.S. and
            Reeves,R.H.
  TITLE     Comparative sequence analysis of 634 kb of the mouse chromosome 16
            region of conserved synteny with the human velocardiofacial
            syndrome region on chromosome 22q11.2
  JOURNAL   Genomics 63 (3), 374-383 (2000)
   PUBMED   10704284
REFERENCE   6  (bases 1 to 2606)
  AUTHORS   Lund,J., Roe,B., Chen,F., Budarf,M., Galili,N., Riblet,R.,
            Miller,R.D., Emanuel,B.S. and Reeves,R.H.
  TITLE     Sequence-ready physical map of the mouse chromosome 16 region with
            conserved synteny to the human velocardiofacial syndrome region on
            22q11.2
  JOURNAL   Mamm Genome 10 (5), 438-443 (1999)
   PUBMED   10337614
REFERENCE   7  (bases 1 to 2606)
  AUTHORS   Kerr,S.M., Vambrie,S., McKay,S.J. and Cooke,H.J.
  TITLE     Analysis of cDNA sequences from mouse testis
  JOURNAL   Mamm Genome 5 (9), 557-565 (1994)
   PUBMED   8000140
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC087802.13.
            
            On Dec 20, 2017 this sequence version replaced XM_006522040.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189670.71071.1,
                                           SRR7345562.767525.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                AC087802.13        141287-141360
            75-337              AC087802.13        146050-146312
            338-503             AC087802.13        153370-153535
            504-1222            AC087802.13        158068-158786
            1223-1415           AC087802.13        165869-166061
            1416-1649           AC087802.13        170803-171036
            1650-2606           AC087802.13        174092-175048
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 11.01 cM"
     gene            1..2606
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /note="kelch-like 22"
                     /db_xref="GeneID:224023"
                     /db_xref="MGI:MGI:1337995"
     exon            1..74
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /inference="alignment:Splign:2.1.0"
     exon            75..337
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..2015
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /note="isoform 1 is encoded by transcript variant 2;
                     kelch-like protein 22"
                     /codon_start=1
                     /product="kelch-like protein 22 isoform 1"
                     /protein_id="NP_001345997.1"
                     /db_xref="CCDS:CCDS49783.1"
                     /db_xref="GeneID:224023"
                     /db_xref="MGI:MGI:1337995"
                     /translation="MAEEQDFAQLCRLPTQPSHSHCVNNTYRSTQHSQALLRGLLALR
                     DSGILFDVVLVVEGKHIEAHRILLAASCDYFRGMFAGGLKEMEQEEVLIHGVSYNAMC
                     QILHFIYTSELELSLSNVQETLVAACQLQIPEIIHFCCDFLMSWVDEENILDVYRLAD
                     LFDLNHLTQQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCETEVYEGAL
                     LYHYSLEQVQADQISLNEPPKLLETVRFPLMEAEVLQRLHDKLGPSPLRDTVASALMY
                     HRNEILQPSLQGPQTELRSDFQCVVGFGGIHSTPSTILSDQAKYLNPLLGEWKHFTAS
                     LAPRMSNQGIAVLNNFVYLIGGDNNVQGFRAESRCWRYDPRHNRWFQIQSLQQEHADL
                     CVCVVGKYIYAVAGRDYHNDLSAVERYDPATNSWDYVAPLKKEVYAHAGTTLQGKMYI
                     TCGRRGEDYLKETHCYDPGSNTWHTLADGPVRRAWHGMAALLDKLFVIGGSNNDAGYR
                     RDVHQVACYSCTSRQWSSVCPLPAGHGEPGIAVLDSRIYVLGGRSHNRGSRTGYVHIY
                     DMEKDCWEEGPQLNNSISGLAACVLTLPRSLLHEQPRGTPNRSQADADFASEVMSVSD
                     WEEFDNSSED"
     exon            338..503
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /inference="alignment:Splign:2.1.0"
     exon            504..1222
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1415
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1649
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..2606
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2574..2579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /note="hexamer: AATAAA"
     polyA_site      2606
                     /gene="Klhl22"
                     /gene_synonym="2610318I18Rik; Kelchl"
                     /note="major polyA site"
ORIGIN      
        1 gaggctccgc ccccacctcc agcctcccgc ctcccgcctg ctgcgagagc ggcctcagct
       61 tgcggcagca ccagctgact acagtagtga gggctgctgt ggctgtcagg atggctgagg
      121 agcaggactt tgcccagctg tgcaggctgc ccacacagcc ctcccactcc cactgtgtca
      181 acaataccta tcgaagcaca cagcactccc aggccctgct ccggggcctg ctggctttgc
      241 gagacagtgg gatcctcttt gacgttgtcc tggtggtaga ggggaagcac attgaggccc
      301 atcggattct gctagctgca tcgtgtgatt atttcagagg aatgtttgct gggggattga
      361 aggagatgga gcaggaagag gtcttgatcc acggtgtgtc ttacaatgcc atgtgccaaa
      421 tcctgcactt catatacact tctgaactag agctcagcct gagcaatgtg caggagaccc
      481 tggttgctgc ctgccaactt cagataccag aaattatcca cttctgctgt gatttcctca
      541 tgtcatgggt agatgaggag aatatccttg atgtctaccg gctagcagac ctctttgact
      601 tgaaccatct gacccaacag ctagatacct acatcctcaa aaactttgtg gccttctcca
      661 gaactgacaa gtaccgccag cttcccttgg agaaggtcta ctctctcctc agcagcaatc
      721 gcctggaggt ttcctgtgag actgaagtgt atgaaggggc cctgctctac cattactccc
      781 tagagcaggt gcaggctgac cagatctcac tgaatgagcc ccccaagctc ctagagactg
      841 tgcgctttcc cctgatggaa gctgaggtcc ttcagcggct gcatgacaag cttggtccta
      901 gcccactgag ggacacagtg gccagtgccc ttatgtacca ccggaatgag atcctgcagc
      961 ccagccttca gggcccacag acagagcttc gctcggactt ccagtgtgtt gtgggctttg
     1021 ggggtattca ctccacaccg tctacaatcc tcagtgacca ggccaagtac ctgaatcctc
     1081 tactgggaga gtggaaacac ttcactgcct ctctggcgcc ccgaatgtcc aatcagggca
     1141 ttgcagtgct caacaacttc gtgtacttaa ttggagggga caataatgtc caaggatttc
     1201 gagctgagtc ccgatgctgg aggtatgacc caaggcacaa tcgctggttc cagatccagt
     1261 ccttgcagca agaacatgca gacctatgtg tgtgtgtagt gggcaagtac atctatgctg
     1321 tggcaggccg tgactaccac aatgacctga gtgctgtgga gcgctatgac cctgccacca
     1381 actcctggga ctatgtggcc ccactcaaga aggaggtata tgcccatgca ggcaccacac
     1441 tgcaggggaa gatgtatatt acctgtggtc gcagaggaga ggactacctg aaagagacac
     1501 actgctatga cccaggaagc aacacgtggc atactctggc tgacgggcct gtgcgacggg
     1561 cctggcatgg catggctgcc ctcctcgaca agctgtttgt gattggaggc agcaacaatg
     1621 atgctggcta cagaagggat gtgcaccagg tggcttgcta cagttgtaca tctcgtcagt
     1681 ggtcatcggt ctgcccactc ccagctgggc atggtgagcc tggcattgct gtgctagaca
     1741 gcaggatata tgtgctgggt ggccgctctc acaaccgagg cagccgtaca ggctacgtgc
     1801 acatctacga catggagaaa gactgctggg aagagggacc ccagctgaac aattccatct
     1861 caggcctggc agcctgtgtg ctcaccctgc ctcgatccct gctccatgag caaccccgag
     1921 ggacccccaa ccgtagccag gcagatgcag actttgcttc tgaggtgatg agtgtgtctg
     1981 actgggaaga gtttgacaac tctagcgagg actagggctc tccccagtgt ttgggtgttc
     2041 aggaagggta atgggcagtg aaggccgtag agcctgggtt agaacaccag gctctagggg
     2101 aggggcccct ccttaagcca tcctggttca ccagctgccc ctttcactct gtttcttcaa
     2161 ccagccagct gccagcattt gtaaactctg tggctgtgaa ttacattaca aagaaaagtg
     2221 ctggggggaa ggctgctatc atcagaggga atgacccatc ctctataaac tctgaatgag
     2281 tgggatctat ttggccgctt cttctcccta gtctggtccc tgatgggtta agtaaggtcc
     2341 ttctttcaag agatcctggg gagagcactc ctagccatag ccaaagtttg tggctccctt
     2401 tctgtgaacc gtggtcagtc tggtgagcag cgccagctgc ctgcagccag ggccttgagc
     2461 actggggagc tctcaaggta tactcttaga gtaactatat acctgtaact ggacctggag
     2521 atgaagagat accctcattg ctctgtgtct gacccaatat tgttgaaaaa tgaaataaag
     2581 cattatgcaa aaggtgttac atctaa
//
