LOCUS       NM_001039482            4037 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus kelch-like 20 (Klhl20), mRNA.
ACCESSION   NM_001039482
VERSION     NM_001039482.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Liu,C.C., Lin,Y.C., Chen,Y.H., Chen,C.M., Pang,L.Y., Chen,H.A.,
            Wu,P.R., Lin,M.Y., Jiang,S.T., Tsai,T.F. and Chen,R.H.
  TITLE     Cul3-KLHL20 Ubiquitin Ligase Governs the Turnover of ULK1 and VPS34
            Complexes to Control Autophagy Termination
  JOURNAL   Mol Cell 61 (1), 84-97 (2016)
   PUBMED   26687681
  REMARK    GeneRIF: Study identifies a key role of Cul3-KLHL20 in autophagy
            termination by controlling autophagy-dependent turnover of ULK1 and
            VPS34 complex subunits and reveals the pathophysiological functions
            of this autophagy termination mechanism.
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Kather,J.N., Friedrich,J., Woik,N., Sticht,C., Gretz,N.,
            Hammes,H.P. and Kroll,J.
  TITLE     Angiopoietin-1 is regulated by miR-204 and contributes to corneal
            neovascularization in KLEIP-deficient mice
  JOURNAL   Invest Ophthalmol Vis Sci 55 (7), 4295-4303 (2014)
   PUBMED   24917145
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4037)
  AUTHORS   Woik,N., Dietz,C.T., Schaker,K. and Kroll,J.
  TITLE     Kelch-like ECT2-interacting protein KLEIP regulates late-stage
            pulmonary maturation via Hif-2alpha in mice
  JOURNAL   Dis Model Mech 7 (6), 683-692 (2014)
   PUBMED   24785085
REFERENCE   4  (bases 1 to 4037)
  AUTHORS   Hahn,N., Dietz,C.T., Kuhl,S., Vossmerbaeumer,U. and Kroll,J.
  TITLE     KLEIP deficiency in mice causes progressive corneal neovascular
            dystrophy
  JOURNAL   Invest Ophthalmol Vis Sci 53 (6), 3260-3268 (2012)
   PUBMED   22511632
  REMARK    GeneRIF: KLEIP is an important molecule regulating corneal
            epithelial integrity
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4037)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY020135.1, AK220547.1, AK050964.1 and AC138640.7.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK220547.1, SRR1660819.198292.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                BY020135.1         3-23
            22-2310             AK220547.1         1-2289
            2311-2460           AK220547.1         2291-2440
            2461-3841           AK050964.1         190-1570
            3842-4037           AC138640.7         101517-101712
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 69.75 cM"
     gene            1..4037
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /note="kelch-like 20"
                     /db_xref="GeneID:226541"
                     /db_xref="MGI:MGI:2444855"
     exon            1..117
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            118..181
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..1988
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /note="Kelch-like ECT2 interacting protein; kelch-like
                     ECT2-interacting protein"
                     /codon_start=1
                     /product="kelch-like protein 20"
                     /protein_id="NP_001034571.1"
                     /db_xref="CCDS:CCDS35748.1"
                     /db_xref="GeneID:226541"
                     /db_xref="MGI:MGI:2444855"
                     /translation="MRRCTNIRPGETGMDVTSRCTLGDPNKLPEGVPQPARMPYISDK
                     HPRQTLEVINLLRKHRELCDVVLVVGAKKIYAHRVILSACSPYFRAMFTGELAESRQT
                     EVVIRDIDERAMELLIDFAYTSQITVEEGNVQTLLPAACLLQLAEIQEACCEFLKRQL
                     DPSNCLGIRAFADTHSCRELLRIADKFTQHNFQEVMESEEFMLLPANQLIDIISSDEL
                     NVRSEEQVFNAVMAWVKYSIQERRPQLPQVLQHVRLPLLSPKFLVGTVGSDPLIKSDE
                     ECRDLVDEAKNYLLLPQERPLMQGPRTRPRKPIRCGEVLFAVGGWCSGDAISSVERYD
                     PQTNEWRMVASMSKRRCGVGVSVLDDLLYAVGGHDGSSYLNSVERYDPKTNQWSSDVA
                     PTSTCRTSVGVAVLGGFLYAVGGQDGVSCLNIVERYDPKENKWTRVASMSTRRLGVAV
                     AVLGGFLYAVGGSDGTSPLNTVERYNPQENRWHTIAPMGTRRKHLGCAVYQDMIYAVG
                     GRDDTTELSSAERYNPRTNQWSPVVAMTSRRSGVGLAVVNGQLMAVGGFDGTTYLKTI
                     EVFDPDANTWRLYGGMNYRRLGGGVGVIKMTHCESHIW"
     exon            182..755
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            756..914
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1009
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1125
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1309
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1453
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1587
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1796
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1903
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..4037
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3999..4004
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /note="hexamer: AATAAA"
     polyA_site      4024
                     /gene="Klhl20"
                     /gene_synonym="D930050H05Rik; Kleip; mKIAA4210"
                     /note="major polyA site"
ORIGIN      
        1 agggaaagga aatcgctgca ggagccgcat cctgcctgcg ctagcggctc cagaggtgga
       61 ggcagagagg gcggagaagg cagagagggt gccgcagaga gaaaagtgtc aaagccagta
      121 cggctttaga acacggtgaa ggatactttt catggtgcag ggaaggaaag ccaatgcgca
      181 ggtgtaccaa cattcgacca ggagagactg gaatggatgt aacaagccgc tgcacccttg
      241 gagaccccaa caagctgcca gaaggggttc cccaacctgc tcgcatgccc tatatttcag
      301 acaagcaccc tcggcaaacc ttggaagtga tcaaccttct gagaaagcac cgggagctat
      361 gtgatgtggt gctagttgtg ggcgctaaga agatatatgc ccaccgcgtc atcctgtcag
      421 cctgcagccc ctacttccga gcaatgttta caggggagct ggcagaaagc cggcagacag
      481 aagtagtgat ccgagacata gatgagaggg ccatggagct cctgattgac tttgcatata
      541 cctcccagat cactgtggaa gagggcaatg ttcagactct cttgcctgct gcttgcctcc
      601 tccagctggc agaaatacag gaagcctgct gtgaattctt aaagagacaa ttagatcctt
      661 ctaattgtct gggcattcgg gcttttgctg acacacattc ttgccgtgag ttgctaagga
      721 ttgcagacaa gttcacccaa cataactttc aagaggtaat ggagagtgaa gaattcatgt
      781 tacttccggc caaccaactc attgacataa tatctagtga tgagctcaat gttcggagtg
      841 aagaacaagt gttcaatgca gtgatggcct gggtcaaata cagtattcag gaaagacggc
      901 ctcagttacc ccaggtgctg caacatgttc gcctgccttt gcttagtccc aagttcctgg
      961 tgggcaccgt aggctctgac cccctcatta aaagtgatga agaatgcaga gacttggtag
     1021 atgaggctaa aaactacctc ctgttgcctc aagaacgacc actaatgcaa gggccaagga
     1081 caagaccacg gaaacctatc cgatgcggag aagtactctt tgcagttggt ggttggtgca
     1141 gtggagatgc catttccagt gttgaacgct atgaccccca gaccaatgag tggcgaatgg
     1201 tagcttcaat gagcaaaagg cgatgtggag tcggggtcag tgttcttgat gacctgttat
     1261 atgcagtagg cggccatgat ggatcctcat atctcaatag tgttgaaaga tatgacccca
     1321 aaactaacca atggagcagc gatgtggccc ctacaagcac ctgcaggaca agcgttggtg
     1381 tggcagtcct tggaggtttt ctctatgctg tgggtggaca ggatggtgtc tcttgcctca
     1441 atatagttga gaggtatgat ccaaaggaga acaagtggac ccgggtggct tcaatgagca
     1501 ctagaaggct aggggtggct gtcgccgtgt tgggagggtt cttatacgct gttggtggct
     1561 cagatggaac atctccactt aacacagtgg agcgctacaa tcctcaggaa aacagatggc
     1621 acaccatagc gcctatgggg acccgaagaa aacacctagg ctgtgcggtg taccaggaca
     1681 tgatctatgc agtgggaggg agagacgaca ctacagagct tagcagtgct gagagataca
     1741 accccagaac taaccagtgg tctcccgtgg tggccatgac atcccgcagg agtggagtcg
     1801 gcctggccgt ggtcaatgga cagcttatgg cagtgggggg ctttgatggc acaacatact
     1861 tgaagaccat tgaagttttt gacccagatg ccaatacgtg gaggttatac ggagggatga
     1921 attaccgtcg gctaggaggt ggtgtaggag ttattaaaat gacacattgt gaatcccata
     1981 tatggtgaac acaggaaaaa aatcttgcat atgctccttt atatttggaa gagccttgac
     2041 ttcgaagctt tgtacagctg gagaaaacaa tagaacaaat tttattcttt gccggtgcct
     2101 caacattcag aaatacccgt ctaaggacgg agctcacctg caaaacgccg cctttgcaca
     2161 atgcctttcc gctctgtgca gaagagtatt tatttttggc tttaatttat catggagttt
     2221 tgatggggat ttttgttttt gttttgttgg tttgggtcct ttgggctttt gttgttgtta
     2281 ttgttgtgtt gtttttaatt tttttttttt aacttatcct tcctaccatg aaaaaaaagg
     2341 aggggggaaa aaggcaaaaa ttccaagctg caaaattttc tttgctttat aggttttgga
     2401 ttggttttaa agatcctggt aagcgcagaa gacctatgtg tgacctgatg ttattccttt
     2461 tagacatatc tatacatctg cagagattgc cagtgatgaa ggtaaaacgc tagtatatgc
     2521 gctcgttacc actttgcact aaatagtaga gcactccaaa tgtaaagctg gttcagtgag
     2581 agtgcgtgct gcttctgcag aggacccgag ttcagttccc agcacttata tctggcagct
     2641 cacaactgtg tgtaattcca tgaacaacgg tggctctcag ccttcctgat gctgtagccc
     2701 tttaacacag ttcctcacgt cgtgctgacc cccaaccata acattatttt tgttgctact
     2761 tcataacagt cattttgctg ctgttatgaa tcatgatgta gacatctgat tacagtatat
     2821 ctgatatgta actcatgtga aagggccctt tgacccccct acccacgcgc cccacccccg
     2881 aagaggtcac gacccacagg ttaaaaactg ctgaacttgc ttatccaggg ctcactttcg
     2941 tcctttgtag gtacccgcat tcctacgcat tcatatacat acaaacacac atccacccat
     3001 caaatataag taattaaaaa tgaaatttct tttaaatgta aaagaaaaac tgaataagaa
     3061 gacatctcac taaacaggaa aattaggaag gactattaag tctataaaag caggactcag
     3121 atgctacact ttgcattcac tggtgggcat tttcatctgg ctagctcaac tttattctta
     3181 ccccagttcc cttttttctc ctgtaatatt ttcatagttt ctgccacatg tatctaggtc
     3241 tcttataaaa gcaatgtgag gctcattggt tgtcatcttg agaattccag tgaaggcgag
     3301 tgtggctgtg gaagagttgt gccgactgtc accacctgca gctttctttc ctgtctcctc
     3361 ttggccacca aggagcagtg aactttctcc agccgtgaat tctgattttc actttacact
     3421 ggatgttctg gcctggtgac tctgaggtga cccttatata caccaaagga ctgatcttca
     3481 tgttagagta catgtttagg agcaccacgc cagcgaagtg tgtggacaga gctggggtcc
     3541 ttacgctgag tgtcactctt catctcagaa ggccactgtt actctgtcct taggctatca
     3601 ctcttcatct cagaagacca ctgttactct gtccctatgc tgagtgtcac tctttcatct
     3661 cagaagacca ctgttactct gtctcttaag ttgcactttc tgatgaaaag ttaaacattt
     3721 taaggaaata aatgaatatt ttagtgccta aggttctccc ccattatagt cacagactga
     3781 tttttttttt ctttcagtgt attttgcagg aaaatctttg aatgagactt tagccacaac
     3841 caaaaggaat aacctttacc ttctccctct cccctggcac cttttgtgtt tacatttaaa
     3901 catcatttgc acctttttca aggaaaaaaa taagtatttg gtaaacaatt gtttacatgt
     3961 atcatttatg cttttttcta gcatgtataa ctttttaaaa taaaggtagt atttaccata
     4021 aaaatgtttt aattttt
//
