LOCUS       NM_178633               3319 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus kelch-like 2, Mayven (Klhl2), mRNA.
ACCESSION   NM_178633 XM_001475462
VERSION     NM_178633.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Kasagi,Y., Takahashi,D., Aida,T., Nishida,H., Nomura,N., Zeniya,M.,
            Mori,T., Sasaki,E., Ando,F., Rai,T., Uchida,S. and Sohara,E.
  TITLE     Impaired degradation of medullary WNK4 in the kidneys of KLHL2
            knockout mice
  JOURNAL   Biochem Biophys Res Commun 487 (2), 368-374 (2017)
   PUBMED   28414128
  REMARK    GeneRIF: Accordingly, medullary WNK4 protein levels were
            significantly increased in the kidneys of KLHL2(-/-) mice. KLHL2 is
            indeed a physiological regulator of WNK4 in vivo; however, its
            function might be different from that of KLHL3 because KLHL2 mainly
            localized in medulla.
REFERENCE   3  (bases 1 to 3319)
  AUTHORS   Zhang,C., Meermeier,N.P., Terker,A.S., Blankenstein,K.I.,
            Singer,J.D., Hadchouel,J., Ellison,D.H. and Yang,C.L.
  TITLE     Degradation by Cullin 3 and effect on WNK kinases suggest a role of
            KLHL2 in the pathogenesis of Familial Hyperkalemic Hypertension
  JOURNAL   Biochem Biophys Res Commun 469 (1), 44-48 (2016)
   PUBMED   26607111
  REMARK    GeneRIF: These results suggest that KLHL2 likely plays a role in
            the pathogenesis of FHHt, and aggravates the phenotype caused by
            mutations in CUL3 and WNK4.
REFERENCE   4  (bases 1 to 3319)
  AUTHORS   Zeniya,M., Morimoto,N., Takahashi,D., Mori,Y., Mori,T., Ando,F.,
            Araki,Y., Yoshizaki,Y., Inoue,Y., Isobe,K., Nomura,N., Oi,K.,
            Nishida,H., Sasaki,S., Sohara,E., Rai,T. and Uchida,S.
  TITLE     Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3
            Signaling in the Regulation of Vascular Tonus
  JOURNAL   J Am Soc Nephrol 26 (9), 2129-2138 (2015)
   PUBMED   25556166
  REMARK    GeneRIF: This study showed that KLHL2 is involved in the
            physiologic regulation of the WNK signal cascade.
REFERENCE   5  (bases 1 to 3319)
  AUTHORS   Tseng,L.A. and Bixby,J.L.
  TITLE     Interaction of an intracellular pentraxin with a BTB-Kelch protein
            is associated with ubiquitylation, aggregation and neuronal
            apoptosis
  JOURNAL   Mol Cell Neurosci 47 (4), 254-264 (2011)
   PUBMED   21549840
REFERENCE   6  (bases 1 to 3319)
  AUTHORS   Chiellini,C., Costa,M., Novelli,S.E., Amri,E.Z., Benzi,L.,
            Bertacca,A., Cohen,P., Del Prato,S., Friedman,J.M. and Maffei,M.
  TITLE     Identification of cathepsin K as a novel marker of adiposity in
            white adipose tissue
  JOURNAL   J Cell Physiol 195 (2), 309-321 (2003)
   PUBMED   12652657
REFERENCE   7  (bases 1 to 3319)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 3319)
  AUTHORS   Soltysik-Espanola,M., Rogers,R.A., Jiang,S., Kim,T.A., Gaedigk,R.,
            White,R.A., Avraham,H. and Avraham,S.
  TITLE     Characterization of Mayven, a novel actin-binding protein
            predominantly expressed in brain
  JOURNAL   Mol Biol Cell 10 (7), 2361-2375 (1999)
   PUBMED   10397770
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC242529.3, AC140351.2 and AC152168.2.
            
            On Apr 29, 2014 this sequence version replaced NM_178633.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031144.1, AK167903.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-292               AC242529.3         45912-46203         c
            293-418             AC242529.3         30722-30847         c
            419-525             AC242529.3         19270-19376         c
            526-647             AC242529.3         7959-8080           c
            648-810             AC140351.2         19862-20024
            811-920             AC152168.2         13664-13773
            921-1037            AC152168.2         18047-18163
            1038-1187           AC152168.2         19655-19804
            1188-1305           AC152168.2         23494-23611
            1306-1503           AC152168.2         25050-25247
            1504-1605           AC152168.2         26066-26167
            1606-1734           AC152168.2         28073-28201
            1735-1875           AC152168.2         28724-28864
            1876-2019           AC152168.2         34798-34941
            2020-3319           AC152168.2         36922-38221
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 32.3 cM"
     gene            1..3319
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /note="kelch-like 2, Mayven"
                     /db_xref="GeneID:77113"
                     /db_xref="MGI:MGI:1924363"
     exon            1..292
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..2048
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /codon_start=1
                     /product="kelch-like protein 2"
                     /protein_id="NP_848748.2"
                     /db_xref="CCDS:CCDS85544.1"
                     /db_xref="GeneID:77113"
                     /db_xref="MGI:MGI:1924363"
                     /translation="METPPLPPACTKQGHQKPLDSKDENPEKHCPLTVNPWHMKKAFK
                     VMNELRSQNLLCDVTIVAEDMEIPAHRVVLAACSPYFHAMFTGEMSESRAKRVRIKEV
                     DGWTLRMLVDYVYTAEIQVTEENVQVLLPAAGLLQLQDVKKTCCEFLESQLHPVNCLG
                     IRAFADMHACTDLLNKANTYAEQHFADVVLSEEFLNLGIEQVCSLISSDKLTISSEEK
                     VFEAVIAWVNHDKDVRQEFMARLMEHVRLPLLPREYLVQRVEEEALVKNSSACKDYLI
                     EAMKYHLLPTEQRMLMKSVRTRLRTPMNLPKLMVVVGGQAPKAIRSVECYDFKEERWH
                     QVAELPSRRCRAGMVYMAGLVFAVGGFNGSLRVRTVDSYDPVKDQWTSVANMRDRRST
                     LGAAVLNGLLYAVGGFDGSTGLSSVEAYNIKSNEWFHVAPMNTRRSSVGVGVVGGLLY
                     AVGGYDGASRQCLSTVECYNATANEWTYIAEMSTRRSGAGVGVLNNLLYAVGGHDGPL
                     VRKSVEVYDPTTNAWRQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPT
                     TDKWTVVSSCMSTGRSYAGVTVIDKPL"
     exon            293..418
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            419..525
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            526..647
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            648..810
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            811..920
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1037
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1187
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1305
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1503
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1605
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1734
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1875
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..2019
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..3319
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2376..2381
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /note="hexamer: AATAAA"
     polyA_site      2403
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /note="major polyA site"
     regulatory      2547..2552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /note="hexamer: AATAAA"
     polyA_site      2567
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
     regulatory      3114..3119
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /note="hexamer: AATAAA"
     polyA_site      3144
                     /gene="Klhl2"
                     /gene_synonym="6030411N21Rik; 8530402H02Rik; ABP-KELCH;
                     Mav"
                     /note="major polyA site"
ORIGIN      
        1 cgcctgtccc gccgccgccg ccgccgccgt cgccgcccgg cactcggggg agcgcgggag
       61 ccgcgccagg cacacgagcg cgcggggccg acgggccatg gccgcggggg ccgccgccgc
      121 aggtggtggc gcgcggtgag gagcgctgcg ccccctcggg gcggatggaa cggggctggg
      181 cgggcggaca gtgccggcgt ccgcggctgg tatggtgctg gcggcggcgg tcgctgcctg
      241 cgttctgaag cccgcgagga gccacaatgg agacgccgcc actgcctccc gcatgcacaa
      301 agcagggtca tcagaagccc ctcgattcaa aagacgagaa tcctgagaaa cattgcccac
      361 tgacggtgaa cccctggcac atgaagaaag cctttaaagt catgaacgaa ctcagaagtc
      421 aaaatctgct gtgtgacgtc acgattgtgg cagaagacat ggagatccct gctcaccgcg
      481 tggtgctggc ggcctgcagt ccgtacttcc acgccatgtt tacaggtgag atgagcgaga
      541 gccgagcaaa gagagttcga ataaaggagg tggacggctg gaccctgagg atgctggtcg
      601 attacgttta cacggcagag attcaggtca cagaggagaa cgtgcaggtc ctcctcccag
      661 cagctggcct gctacagtta caggatgtga agaagacatg ctgtgaattt ttagaatccc
      721 agcttcaccc tgtcaactgc ttaggaatcc gggcttttgc tgatatgcat gcatgcacag
      781 acctcctgaa taaggccaac acgtatgcag aacaacattt tgcagatgtc gtacttagtg
      841 aagagtttct taacctcggc atcgagcagg tgtgcagctt aatctccagt gataaactca
      901 ccatttcctc cgaggagaag gtatttgaag cagtcatcgc ctgggtaaac cacgataagg
      961 acgtaagaca ggagttcatg gctcggctca tggagcatgt ccgattacct ctgcttcctc
     1021 gggagtactt ggtgcagcgg gttgaagagg aagcattggt gaagaacagc agtgcgtgca
     1081 aagattacct cattgaagcc atgaagtacc acctgctgcc gacagagcag cgcatgttaa
     1141 tgaagagcgt gcggacccgc ctgaggacgc ccatgaacct tcccaaattg atggtggtgg
     1201 tcgggggcca ggcaccaaag gctatccgaa gtgtggaatg ctacgacttc aaagaagaac
     1261 ggtggcacca ggtggcagag ctgccttcca ggaggtgcag ggcagggatg gtctatatgg
     1321 ctgggctggt ctttgctgtt ggtggcttca atggctcact aagagttcgc actgtagatt
     1381 cctatgaccc cgtgaaagac cagtggacca gcgtggcgaa catgcgagac cgaagaagca
     1441 cacttggagc cgcggtgctg aatgggctgc tgtacgccgt ggggggcttt gacggcagca
     1501 caggtctatc gtctgtggaa gcatacaaca taaaatctaa tgagtggttt catgtcgccc
     1561 caatgaacac gaggaggagc agtgtgggtg ttggcgttgt tggaggtttg ctctatgccg
     1621 tggggggtta cgatggagca tcgcgccagt gtcttagcac agtagaatgc tataatgcta
     1681 ctgcaaacga gtggacttac atagcagaaa tgagcaccag acggagcgga gcaggtgttg
     1741 gtgtgttaaa caacttatta tatgctgtag gaggtcatga tggtccttta gtacgaaaaa
     1801 gtgttgaagt atatgacccc accacaaatg cctggagaca ggttgcagat atgaacatgt
     1861 gcagaagaaa tgcaggtgtc tgtgcagtta atggtctgtt gtatgttgtc ggaggagatg
     1921 atggttcctg taacttggcg tcggtagagt attataaccc caccacggat aaatggacag
     1981 ttgtgtcctc ttgtatgagc acggggagaa gctacgcagg ggtcacagtt attgataaac
     2041 cgttatgagc ctgaaggaca ttttcagcgt atcattccat gagaaccagg cgtccacaag
     2101 tatttctgaa gggactgagc gcctggcctc tccacttgta gccgcacttt acgtctcagc
     2161 agaagatgag accatctgcc ttcagaggcc tcggatactg aagattattt ttggtagaag
     2221 cactgtgtag gctttctctg caccgagcag cagctgactg actttacaag agacctagac
     2281 tgcagtccct ccactgtggt ctttaggcaa cagtcgcttt cataaaaact agttcttatc
     2341 agcctcgaat gactatggat tattttgtga aagagaataa agttaacgtg tgttcttgta
     2401 aaattaagtt ttatctttat tctttctcct gaaaaatctc ctgggaacag agaatctttg
     2461 gagcagatta aaatctatgt aagtatatga cactgttgag gggtgaaact gctggctttt
     2521 ataagtatct ttgattacat gactataata aattgtgtgc atataaatgt gtgtctatat
     2581 gctttccttt aaatatgttt gaaaagatgt ttgattatct atttataatt ggcacagtac
     2641 tttgaattat gccagtacta cattgtaaaa cagagttgta ttttttgata tttaacaatg
     2701 ctgaacactt taaaaaacgc cacttctgag gaatgaatga acatggcgta acacacttta
     2761 atatgtgtga tgccaaactt ttttaaataa aatataaatt atgcttattt attatttttt
     2821 tctttagttt aatcttgatc atgttttggt gtgtgttttt aatttttttt cttaaagtaa
     2881 cacttttggc atgaacatac ctgcaggttt tttgatgaat ataatgaatg tgtggaattc
     2941 aactgaattt gcatggtctt aagaattttt tctgtgtgta taaatttagc tgctattaat
     3001 ggaagagaaa gctttctgtg actaatcatg tgtattgagc agatagtttt tctgagatct
     3061 tcgattaatc tctttaaaga tgaccaacat gtgtctttct tgaattatct ttgaataaaa
     3121 gtttgtatat tatttgtttc acaacgtcct tggttttacc agtgtgactt tttgactatt
     3181 ccttttgcag accttgggtt ctctgacttc atacattgat accattattt ttcatgtttg
     3241 gaacaattct gtgttttcat ttttatttat tttttgctaa agtaacttgt acactaaatc
     3301 tattaaagaa ataaccaat
//
