LOCUS       NM_025727               2040 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus kelch-like 10 (Klhl10), transcript variant 1, mRNA.
ACCESSION   NM_025727
VERSION     NM_025727.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Huang,Z., Chen,F., Xie,M., Zhang,H., Zhuang,Y., Huang,C., Li,X.,
            Liu,H. and Chen,Z.
  TITLE     The I510V mutation in KLHL10 in a patient with
            oligoasthenoteratozoospermia
  JOURNAL   J Reprod Dev 67 (5), 313-318 (2021)
   PUBMED   34433733
  REMARK    GeneRIF: The I510V mutation in KLHL10 in a patient with
            oligoasthenoteratozoospermia.
REFERENCE   3  (bases 1 to 2040)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2040)
  AUTHORS   Lamberti,A., Sanges,C., Chambery,A., Migliaccio,N., Rosso,F., Di
            Maro,A., Papale,F., Marra,M., Parente,A., Caraglia,M.,
            Abbruzzese,A. and Arcari,P.
  TITLE     Analysis of interaction partners for eukaryotic translation
            elongation factor 1A M-domain by functional proteomics
  JOURNAL   Biochimie 93 (10), 1738-1746 (2011)
   PUBMED   21689717
REFERENCE   5  (bases 1 to 2040)
  AUTHORS   Yan,W., Ma,L., Burns,K.H. and Matzuk,M.M.
  TITLE     Haploinsufficiency of kelch-like protein homolog 10 causes
            infertility in male mice
  JOURNAL   Proc Natl Acad Sci U S A 101 (20), 7793-7798 (2004)
   PUBMED   15136734
  REMARK    GeneRIF: Both alleles are required for male fertility and
            haploinsufficiency prevents genetic transmission of both mutant and
            WT alleles.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL590968.8.
            
            On Mar 10, 2023 this sequence version replaced NM_025727.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC139267.1, AY495337.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-326               AL590968.8         158875-159200
            327-816             AL590968.8         162359-162848
            817-1434            AL590968.8         164097-164714
            1435-1584           AL590968.8         173260-173409
            1585-2040           AL590968.8         173548-174003
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 63.47 cM"
     gene            1..2040
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /note="kelch-like 10"
                     /db_xref="GeneID:66720"
                     /db_xref="MGI:MGI:2181067"
     exon            1..326
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1959
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kelch-like protein 10"
                     /codon_start=1
                     /product="kelch-like protein 10 isoform 1"
                     /protein_id="NP_080003.1"
                     /db_xref="CCDS:CCDS25423.1"
                     /db_xref="GeneID:66720"
                     /db_xref="MGI:MGI:2181067"
                     /translation="MEMESTAASTRFHQPHMERKMSAMTCEIFNELRLEGKLCDVVIK
                     VNGFEFNAHKNILCSCSSYFRALFTSGWNNTEKKVYNIPGISPDMMKLIIEYAYTRTV
                     PITPDNVEKLLAAADQFNIMGIVRGCCEFLKSELCLDNCIGICKFTDYYYCPELRQKA
                     YMFILHNFEEMVKVSAEFLELSVTELKDIIEKDELNVKQEDAVFEAILKWISHDPQNR
                     KQHISVLLPKVRLALMHAEYFMNNVKMNDYVKDSEECKPVIINALKAMYDLNMNGPSN
                     SDFTNPLTRPRLPYAILFAIGGWSGGSPTNAIEAYDARADRWVNVTCEEESPRAYHGA
                     AYLKGYVYIIGGFDSVDYFNSVKRFDPVKKTWHQVAPMHSRRCYVSVTVLSNFIYAMG
                     GFDGYVRLNTAERYEPETNQWTLIAPMHEQRSDASATTLYGKVYICGGFNGNECLFTA
                     EVYNTESNQWTVIAPMRSRRSGIGVIAYGEHVYAVGGFDGANRLRSAEAYSPVANTWR
                     TIPTMFNPRSNFGIEVVDDLLFVVGGFNGFTTTFNVECYDEKTDEWYDAHDMSIYRSA
                     LSCCVVPGLANVGEYAARRDNFTGLALRDEVKYSASTSTLPV"
     exon            327..816
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            817..1434
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1584
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..2040
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1976..1981
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1996
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /note="major polyA site"
     regulatory      2015..2020
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2040
                     /gene="Klhl10"
                     /gene_synonym="4921517C11Rik"
ORIGIN      
        1 acttgggctg cctgatggtc cctatatgaa agatgtagta gggaaaagga gcgacagctg
       61 gctaaagggg ccccccataa ccctccccaa caccccagga gagcagcctc tctcccctgg
      121 tcccaaggag ccatggagat ggagagcacc gctgcctcca cccggttcca ccagcctcac
      181 atggaaagaa agatgagtgc aatgacctgt gagatcttca acgagctccg gcttgagggc
      241 aagctctgcg acgtggtcat caaagtcaat ggcttcgagt tcaatgccca caagaacatc
      301 ctctgtagct gcagctccta ctttagagct ttgtttacaa gtggctggaa caacactgag
      361 aagaaggttt acaacattcc tggcatttcc cccgacatga tgaagctaat tattgagtat
      421 gcgtacactc ggaccgtgcc tatcacccct gacaatgtgg agaagctgct ggccgctgct
      481 gatcagttta acatcatggg catcgttagg ggttgctgtg agttcctcaa gtcggagctg
      541 tgtctggata actgtatcgg catctgcaag ttcacggact actactactg ccccgagctg
      601 aggcagaagg cctacatgtt catcctgcac aacttcgagg agatggtgaa agtctcggca
      661 gagtttctgg agctctctgt cactgagctc aaggacatca ttgagaaaga cgaactcaac
      721 gtgaagcagg aggacgcagt attcgaggcc attttaaagt ggatttctca cgacccccaa
      781 aataggaagc aacatatttc agttttactt cctaaggttc gcctggccct aatgcatgct
      841 gagtacttca tgaataatgt taagatgaat gactatgtca aagacagtga ggaatgtaag
      901 ccagtcatca ttaacgccct aaaggctatg tatgacctga acatgaacgg accctccaat
      961 tctgacttca ccaacccact taccaggccc cgcctgccct atgccatcct attcgctatt
     1021 ggtggctgga gtggcgggag ccccaccaat gccattgagg catacgatgc tcgagcagac
     1081 agatgggtga atgtcacgtg tgaggaagaa agtcctcgag cctaccacgg ggcagcctac
     1141 ttgaaaggct atgtttatat catcgggggg tttgatagcg tagactactt caatagtgtt
     1201 aagcgttttg accctgtgaa gaaaacttgg caccaagtgg cccctatgca ctctagacgt
     1261 tgctatgtca gcgtgacagt cctcagcaat ttcatctatg ccatgggagg gtttgatggc
     1321 tacgtgcgtc tcaacactgc tgaacggtac gagccagaga ccaaccaatg gacactcatt
     1381 gcccccatgc atgagcagcg gagcgatgcg agcgccacaa cactctatgg gaaggtctac
     1441 atatgtggtg ggtttaacgg aaacgaatgc ctgtttacag cagaagtgta caacactgag
     1501 agcaatcaat ggacagtcat tgcacccatg aggagcagga ggagtggaat aggtgtaatt
     1561 gcttacgggg aacatgtata cgcagtgggt ggctttgatg gagccaatcg actgaggagt
     1621 gcagaagcct acagcccagt ggctaatact tggcgcacga tccccactat gtttaaccct
     1681 cgtagcaatt ttgggatcga ggtagtggac gacctcttgt ttgtggtggg tggctttaat
     1741 ggctttacca ccacctttaa cgttgagtgc tatgatgaaa agaccgatga gtggtatgac
     1801 gctcatgaca tgagcatata ccgcagtgct ctgagctgct gcgtggtacc agggctagcc
     1861 aatgttgggg agtatgcagc tagacgggac aacttcacgg gattagcact gcgagatgaa
     1921 gtaaagtatt ccgcttcgac aagtacccta cctgtatgag cctcttcatt tagctaataa
     1981 aaagtctaag caataagaat taattctttt tttaaataaa tgcagtgttt aaacttgtaa
//
