LOCUS       NM_027117               1946 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus kelch domain containing 2 (Klhdc2), mRNA.
ACCESSION   NM_027117
VERSION     NM_027117.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 1946)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 1946)
  AUTHORS   Neuhaus,P., Jaschinsky,B., Schneider,S., Neuhaus,H., Wolter,A.,
            Ebelt,H. and Braun,T.
  TITLE     Overexpression of Kelch domain containing-2 (mKlhdc2) inhibits
            differentiation and directed migration of C2C12 myoblasts
  JOURNAL   Exp Cell Res 312 (16), 3049-3059 (2006)
   PUBMED   16860314
  REMARK    GeneRIF: These results suggest that a tightly controlled expression
            of mKlhdc2 is essential for a faithful execution of the myogenic
            differentiation and migration program.
REFERENCE   5  (bases 1 to 1946)
  AUTHORS   Ishii,H., Mimori,K., Mori,M. and Vecchione,A.
  TITLE     Differentially expressed genes in endothelial differentiation
  JOURNAL   DNA Cell Biol 24 (7), 432-437 (2005)
   PUBMED   16008511
  REMARK    GeneRIF: A conserved but uncharacterized gene encoding Klhdc2,
            which was down-regulated during endothelial differentiation, was
            identified.
REFERENCE   6  (bases 1 to 1946)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099934.14 and AK009481.1.
            
            On Apr 2, 2009 this sequence version replaced NM_027117.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY513272.1, BC005581.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-379               AC099934.14        204933-205311
            380-1416            AK009481.1         295-1331
            1417-1946           AC099934.14        218410-218939
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 28.77 cM"
     gene            1..1946
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /note="kelch domain containing 2"
                     /db_xref="GeneID:69554"
                     /db_xref="MGI:MGI:1916804"
     exon            1..467
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     CDS             315..1535
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /note="endothelial differentiation inhibitory protein TNG"
                     /codon_start=1
                     /product="kelch domain-containing protein 2"
                     /protein_id="NP_081393.2"
                     /db_xref="CCDS:CCDS25950.1"
                     /db_xref="GeneID:69554"
                     /db_xref="MGI:MGI:1916804"
                     /translation="MADGNEDARAEDLPGPAFENYEAMELACPAERSGHVAVSDGRHM
                     FVWGGYKSNQVRGLYDFYLPREELWIYNMETGRWKKINTEGDVPPSMSGSCAVCVDRV
                     LYLFGGHHSRGNTNKFYMLDSRSADRGLQWERIDCQGIPPSSKDKLGVWVYKNKLIFF
                     GGYGYLPEDKVLGTFEFDETSFWNSSHPRGWNDHVHILDTETFAWSQPITTGKAPSPR
                     AAHACATVGNKGFVFGGRYRDARMNDLHYLNLDTWEWNELIPQGVCPVGRSWHSLTPV
                     SSDHLFLFGGFTTEKQPLSDAWTYCISKNEWIQFNHPYVEKPRLWHTACASDEGEVIV
                     FGGCANNLLVHHRAAHSNEVLIFSVQPKSLVRLSLEAVICFKEMLANSWSCLPKHLLH
                     SVNQRFGSNNTSGS"
     exon            468..547
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            548..665
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            666..781
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            782..863
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            864..947
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1028
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1087
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1197
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1270
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1358
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1411
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1946
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1705..1710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /note="hexamer: AATAAA"
     polyA_site      1724
                     /gene="Klhdc2"
                     /gene_synonym="2310022K15Rik; D12Ertd522e; HCLP-1; LCP"
                     /note="major polyA site"
ORIGIN      
        1 gacagtgtcc gctgcggacg acttccggcg gcggcataaa caaggccccg gcggccggag
       61 gcggggcggg ctgcgagtgc gagccccgtc ggccgctctc ccgctctgag ccaggacccg
      121 gccgcggcgc ggtccccgcg agacccgcag ccgctgtcgg cgcgcgcccc agcgctcgtc
      181 ccggaggcgg aggcgtagcg caggacaggc gggtcgtcag cgctggctgc gcgccgcggt
      241 ccaccgctcc gctttccttt gtctcccgtc tgcggggccg gagcggtctc ggtggccagc
      301 ggtgcagcct caagatggct gatggcaacg aggatgctcg agcggaggat ctgccgggcc
      361 cggccttcga gaactacgag gccatggagc tggcctgccc ggctgaacgc agcggccacg
      421 tcgctgtcag cgatgggcgc cacatgttcg tctggggcgg ctacaagagt aatcaagtga
      481 gaggattata tgacttttat ctgcccagag aagaactgtg gatctacaac atggagactg
      541 gaagatggaa gaaaatcaac actgaaggtg acgttccccc gtctatgtct gggagctgcg
      601 ctgtgtgcgt ggaccgggtg ctgtacttgt ttggtgggca ccattccaga ggcaatacaa
      661 acaagttcta catgctggat tcgaggtctg cagacagagg gttacagtgg gaaaggatcg
      721 actgccaagg aatccctcca tcatcaaagg acaaacttgg cgtctgggtg tataaaaaca
      781 agttaatatt ttttggaggc tatggatatt tacctgaaga taaagtattg ggaacttttg
      841 aatttgatga aacatctttt tggaattcaa gtcatccaag aggatggaat gatcatgtcc
      901 atattttaga cactgaaaca tttgcctgga gccagcccat caccactggt aaggcacctt
      961 cacctcgggc tgcccatgcc tgtgcaactg ttggaaacaa gggctttgtg tttggtggca
     1021 gataccggga tgctagaatg aatgatcttc actatcttaa tctggatacg tgggagtgga
     1081 atgaactaat tccacaaggt gtgtgccctg ttggcagatc ctggcactca ctgacaccag
     1141 tctcttcaga ccatcttttt ctctttggag gatttaccac tgaaaaacag ccactaagtg
     1201 atgcctggac ttactgcatc agtaaaaatg aatggattca gtttaatcat ccttatgttg
     1261 aaaaaccaag attatggcac acagcttgtg cgagtgatga aggagaagtc attgtttttg
     1321 gtggttgtgc taacaatctg ctggttcatc acagagctgc acacagtaat gaagtactta
     1381 tattttcagt tcaaccaaaa tctcttgtac ggctaagctt agaagcagtc atctgcttta
     1441 aagagatgct agccaactcg tggagctgcc ttccaaaaca cttacttcac agcgttaatc
     1501 agaggtttgg tagtaacaac acttctggat cataaagctt tgtagataat acttcttctg
     1561 gtccacttgc atggagaaca gccccgtact tgtcacacag tgtcatcctt tgtataatta
     1621 tatgcatcgt tgtagtttgc agcttcctgg gttttatgtg catgtgaatg gcctagagaa
     1681 cctatttttg tgtctaaagt ttacaataaa tgtatttaac accagtacct gtctattgtt
     1741 aaagttaaaa aaaattactg tgttggcttt tttttttttt aacgatttat ttatgtatac
     1801 tgaatggtgt tttcctgcat gcctgagggt gttggattcc ctggaacagg agttacaagc
     1861 tgcaatgtgg gtgctggcaa ctgtacgtgg gccctgtgga agaacagcca gtgctcttaa
     1921 cccctgagcc atctctccaa cccctg
//
