LOCUS       NM_013823               5124 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus klotho (Kl), mRNA.
ACCESSION   NM_013823
VERSION     NM_013823.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Kim,B., Kim,T., Im,H., Shin,K.S. and Kang,S.J.
  TITLE     alpha-Klotho regulates mouse embryonic neural stem cell
            proliferation and differentiation
  JOURNAL   Biochem Biophys Res Commun 742, 151157 (2025)
   PUBMED   39672006
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Dos Santos,G.R.O., Cararo-Lopes,M.M., Possebom,I.R., de Sa Lima,L.,
            Scavone,C. and Kawamoto,E.M.
  TITLE     Sex-dependent changes in AMPAR expression and Na, K-ATPase activity
            in the cerebellum and hippocampus of alpha-Klotho-Hypomorphic mice
  JOURNAL   Neuropharmacology 258, 110097 (2024)
   PUBMED   39094831
  REMARK    GeneRIF: Sex-dependent changes in AMPAR expression and Na, K-ATPase
            activity in the cerebellum and hippocampus of
            alpha-Klotho-Hypomorphic mice.
REFERENCE   3  (bases 1 to 5124)
  AUTHORS   Jankowski,J., Lee,H.K., Liu,C., Wilflingseder,J. and
            Hennighausen,L.
  TITLE     Sexually dimorphic renal expression of mouse Klotho is directed by
            a kidney-specific distal enhancer responsive to HNF1b
  JOURNAL   Commun Biol 7 (1), 1142 (2024)
   PUBMED   39277686
  REMARK    GeneRIF: Sexually dimorphic renal expression of mouse Klotho is
            directed by a kidney-specific distal enhancer responsive to HNF1b.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5124)
  AUTHORS   Dubnov,S., Bennett,E.R., Yayon,N., Yakov,O., Bennett,D.A.,
            Seshadri,S., Mufson,E., Tzur,Y., Greenberg,D., Kuro-O,M.,
            Paldor,I., Abraham,C.R. and Soreq,H.
  TITLE     Knockout of the longevity gene Klotho perturbs aging and
            Alzheimer's disease-linked brain microRNAs and tRNA fragments
  JOURNAL   Commun Biol 7 (1), 720 (2024)
   PUBMED   38862813
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5124)
  AUTHORS   Taheri,N., Choi,E.L., Nguyen,V.T.T., Zhang,Y., Huynh,N.M.,
            Kellogg,T.A., van Wijnen,A.J., Ordog,T. and Hayashi,Y.
  TITLE     Inhibition of EZH2 Reduces Aging-Related Decline in Interstitial
            Cells of Cajal of the Mouse Stomach
  JOURNAL   Cell Mol Gastroenterol Hepatol 18 (4), 101376 (2024)
   PUBMED   38969206
REFERENCE   6  (bases 1 to 5124)
  AUTHORS   Kawaguchi,H., Manabe,N., Miyaura,C., Chikuda,H., Nakamura,K. and
            Kuro-o,M.
  TITLE     Independent impairment of osteoblast and osteoclast differentiation
            in klotho mouse exhibiting low-turnover osteopenia
  JOURNAL   J Clin Invest 104 (3), 229-237 (1999)
   PUBMED   10430604
REFERENCE   7  (bases 1 to 5124)
  AUTHORS   Johnson,F.B., Sinclair,D.A. and Guarente,L.
  TITLE     Molecular biology of aging
  JOURNAL   Cell 96 (2), 291-302 (1999)
   PUBMED   9988222
  REMARK    Review article
REFERENCE   8  (bases 1 to 5124)
  AUTHORS   Yamashita,T., Nifuji,A., Furuya,K., Nabeshima,Y. and Noda,M.
  TITLE     Elongation of the epiphyseal trabecular bone in transgenic mice
            carrying a klotho gene locus mutation that leads to a syndrome
            resembling aging
  JOURNAL   J Endocrinol 159 (1), 1-8 (1998)
   PUBMED   9795335
  REMARK    Review article
REFERENCE   9  (bases 1 to 5124)
  AUTHORS   Shiraki-Iida,T., Aizawa,H., Matsumura,Y., Sekine,S., Iida,A.,
            Anazawa,H., Nagai,R., Kuro-o,M. and Nabeshima,Y.
  TITLE     Structure of the mouse klotho gene and its two transcripts encoding
            membrane and secreted protein
  JOURNAL   FEBS Lett 424 (1-2), 6-10 (1998)
   PUBMED   9537505
REFERENCE   10 (bases 1 to 5124)
  AUTHORS   Kuro-o,M., Matsumura,Y., Aizawa,H., Kawaguchi,H., Suga,T.,
            Utsugi,T., Ohyama,Y., Kurabayashi,M., Kaname,T., Kume,E.,
            Iwasaki,H., Iida,A., Shiraki-Iida,T., Nishikawa,S., Nagai,R. and
            Nabeshima,Y.I.
  TITLE     Mutation of the mouse klotho gene leads to a syndrome resembling
            ageing
  JOURNAL   Nature 390 (6655), 45-51 (1997)
   PUBMED   9363890
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC138259.1 and AB005141.1.
            
            On Apr 22, 2009 this sequence version replaced NM_013823.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB005141.1, BC138258.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4237              BC138259.1         1-4237
            4238-5124           AB005141.1         4146-5032
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 89.77 cM"
     gene            1..5124
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /note="klotho"
                     /db_xref="GeneID:16591"
                     /db_xref="MGI:MGI:1101771"
     exon            1..935
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..3155
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /EC_number="3.2.1.31"
                     /note="secreted form of Klotho protein"
                     /codon_start=1
                     /product="klotho precursor"
                     /protein_id="NP_038851.2"
                     /db_xref="CCDS:CCDS19889.1"
                     /db_xref="GeneID:16591"
                     /db_xref="MGI:MGI:1101771"
                     /translation="MLARAPPRRPPRLVLLRLLLLHLLLLALRARCLSAEPGQGAQTW
                     ARFARAPAPEAAGLLHDTFPDGFLWAVGSAAYQTEGGWRQHGKGASIWDTFTHHSGAA
                     PSDSPIVVAPSGAPSPPLSSTGDVASDSYNNVYRDTEGLRELGVTHYRFSISWARVLP
                     NGTAGTPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDTYGGWANRALADHF
                     RDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSSRLGYLVAHNLLLA
                     HAKVWHLYNTSFRPTQGGRVSIALSSHWINPRRMTDYNIRECQKSLDFVLGWFAKPIF
                     IDGDYPESMKNNLSSLLPDFTESEKRLIRGTADFFALSFGPTLSFQLLDPNMKFRQLE
                     SPNLRQLLSWIDLEYNHPPIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIRLD
                     GVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKELLPKSSALFYQKLIEDNGF
                     PPLPENQPLEGTFPCDFAWGVVDNYVQVDTTLSQFTDPNVYLWDVHHSKRLIKVDGVV
                     AKKRKPYCVDFSAIRPQITLLREMRVTHFRFSLDWALILPLGNQTQVNHTVLHFYRCM
                     ISELVHANITPVVALWQPAAPHQGLPHALAKHGAWENPHTALAFADYANLCFKELGHW
                     VNLWITMNEPNTRNMTYRAGHHLLRAHALAWHLYDDKFRAAQKGKISIALQADWIEPA
                     CPFSQNDKEVAERVLEFDIGWLAEPIFGSGDYPRVMRDWLNQKNNFLLPYFTEDEKKL
                     VRGSFDFLAVSHYTTILVDWEKEDPMKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRK
                     VLNWLRFKYGDLPMYVTANGIDDDPHAEQDSLRIYYIKNYVNEALKAYVLDDINLCGY
                     FAYSLSDRSAPKSGFYRYAANQFEPKPSMKHYRKIIDSNGFLGSGTLGRFCPEEYTVC
                     TECGFFQTRKSLLVFISFLVFTFIISLALIFHYSKKGQRSYK"
     exon            936..1446
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1715
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..2817
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..5118
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4755..4760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /note="hexamer: AATATA"
     polyA_site      4783
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
     regulatory      5097..5102
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /note="hexamer: AATAAA"
     polyA_site      5118
                     /gene="Kl"
                     /gene_synonym="alpha-kl"
                     /note="major polyA site"
ORIGIN      
        1 gataatcatt gctcgtgggg cggcgggagc gggggtgggc accgcgtagg gagggcggcg
       61 gggcgcgggc atataggggc gcggcgcggt gccctcccgg ctcccgcagc atgctagccc
      121 gcgcccctcc tcgccgcccg ccgcggctgg tgctgctccg tttgctgttg ctgcatctgc
      181 tgctgctcgc cctgcgcgcc cgctgcctga gcgctgagcc gggtcagggc gcgcagacct
      241 gggctcgctt cgcgcgcgct cctgccccag aggccgctgg cctcctccac gacaccttcc
      301 ccgacggttt cctctgggcg gtaggcagcg ccgcctatca gaccgagggc ggctggcgac
      361 agcacggcaa aggcgcgtcc atctgggaca ctttcaccca tcactctggg gcggccccgt
      421 ccgactcccc gatcgtcgtg gcgccgtcgg gtgccccgtc gcctcccctg tcctccactg
      481 gagatgtggc cagcgatagt tacaacaacg tctaccgcga cacagagggg ctgcgcgaac
      541 tgggggtcac ccactaccgc ttctccatat cgtgggcgcg ggtgctcccc aatggcaccg
      601 cgggcactcc caaccgcgag gggctgcgct actaccggcg gctgctggag cggctgcggg
      661 agctgggcgt gcagccggtg gttaccctgt accattggga cctgccacag cgcctgcagg
      721 acacctatgg cggatgggcc aatcgcgccc tggccgacca tttcagggat tatgccgagc
      781 tctgcttccg ccacttcggt ggtcaggtca agtactggat caccattgac aacccctacg
      841 tggtggcctg gcacgggtat gccaccgggc gcctggcccc gggcgtgagg ggcagctcca
      901 ggctcgggta cctggttgcc cacaacctac ttttggctca tgccaaagtc tggcatctct
      961 acaacacctc tttccgcccc acacagggag gccgggtgtc tatcgcctta agctcccatt
     1021 ggatcaatcc tcgaagaatg actgactata atatcagaga atgccagaag tctcttgact
     1081 ttgtgctagg ctggtttgcc aaacccatat ttattgatgg cgactaccca gagagtatga
     1141 agaacaacct ctcgtctctt ctgcctgatt ttactgaatc tgagaagagg ctcatcagag
     1201 gaactgctga cttttttgct ctctccttcg gaccaacctt gagctttcag ctattggacc
     1261 ctaacatgaa gttccgccaa ttggagtctc ccaacctgag gcagcttctg tcttggatag
     1321 atctggaata taaccaccct ccaatattta ttgtggaaaa tggctggttt gtctcgggaa
     1381 ccaccaaaag ggatgatgcc aaatatatgt attatctcaa gaagttcata atggaaacct
     1441 taaaagcaat cagactggat ggggtcgacg tcattgggta caccgcgtgg tcgctcatgg
     1501 acggtttcga gtggcatagg ggctacagca tccggcgagg actcttctac gttgactttc
     1561 tgagtcagga caaggagctg ttgccaaagt cttcggcctt gttctaccaa aagctgatag
     1621 aggacaatgg ctttcctcct ttacctgaaa accagcccct tgaagggaca tttccctgtg
     1681 actttgcttg gggagttgtt gacaactacg ttcaagtgga cactactctc tctcagttta
     1741 ctgacccgaa tgtctatctg tgggatgtgc atcacagtaa gaggcttatt aaagtagacg
     1801 gggttgtagc caagaagaga aaaccttact gtgttgattt ctctgccatc cggcctcaga
     1861 taaccttact tcgagaaatg cgggtcaccc actttcgctt ctccctggac tgggccctga
     1921 tcttgcctct gggtaaccag acccaagtga accacacggt tctgcacttc taccgctgca
     1981 tgatcagcga gctggtgcac gccaacatca ctccagtggt ggccctgtgg cagccagcag
     2041 ccccgcacca aggcctgcca catgcccttg caaaacatgg ggcctgggag aacccgcaca
     2101 ctgctctggc gtttgcagac tacgcaaacc tgtgttttaa agagttgggt cactgggtca
     2161 atctctggat caccatgaac gagccaaaca cacggaacat gacctatcgt gccgggcacc
     2221 acctcctgag agcccatgcc ttggcttggc atctgtacga tgacaagttt agggcggctc
     2281 agaaaggcaa aatatccatc gccttgcagg ctgactggat agaaccggcc tgccctttct
     2341 ctcaaaatga caaagaagtg gccgagagag ttttggaatt tgatataggc tggctggcag
     2401 agcctatttt tggttccgga gattatccac gtgtgatgag ggactggctg aaccaaaaaa
     2461 acaattttct tttgccctat ttcaccgaag atgaaaaaaa gctagtccgg ggttcctttg
     2521 acttcctggc ggtgagtcat tacaccacca ttctggtaga ctgggaaaag gaggatccga
     2581 tgaaatacaa cgattacttg gaggtacagg agatgactga catcacatgg ctcaactctc
     2641 ccagtcaggt ggcagtggtg ccttgggggc tgcgcaaagt gctcaactgg ctaaggttca
     2701 agtacggaga cctcccgatg tatgtgacag ccaatggaat cgatgatgac ccccacgccg
     2761 agcaagactc actgaggatc tattatatta agaattatgt gaatgaggct ctgaaagcct
     2821 acgtgttgga cgacatcaac ctttgtggct actttgcgta ttcacttagt gatcgctcag
     2881 ctcccaagtc tggcttttat cgatatgctg cgaatcagtt tgagcccaaa ccatctatga
     2941 aacattacag gaaaattatt gacagcaatg gcttcctggg ttctggaaca ctgggaaggt
     3001 tttgtccaga agaatacact gtgtgcaccg aatgtggatt ttttcaaacc cggaagtctt
     3061 tgctggtctt catctcgttt cttgttttta cttttattat ttctcttgct ctcatttttc
     3121 actactccaa gaaaggccag agaagttata agtaatgtga acgtctgcct ggccattcgc
     3181 tttgggatca agatgtacac gccgtcagcc gtttgcacct ctctgtgttg tgagccgcat
     3241 tccacacatt tcgattctag aaaacccttt ttgtcatggg tggtagaggt tttaaacagg
     3301 aattggtgag aataaaatat tgcagggtga atggtatctg aatctgctct ctttggtggc
     3361 aattacggaa ttatactcac cacagtttct acagtgcccc ggaatggaag gcatagaata
     3421 cggtagggat aacagtgcca agcagacaga agtttaaaga acaactttag ggacttgttt
     3481 atccatggcc atttttaaat tcactcctgt tggggagtaa cactctctca attaccatct
     3541 taacacctgg actttacctg atccagtttt acaaggtgaa gtagaaaaat atccagtaaa
     3601 ggtggccaag agccctgagt ccagagcagc ccattaagaa gcactattcc taccaaatgc
     3661 tgctaatgtc aatttacaaa tatacttaga aagcacatta tggacatttg tattcttgtg
     3721 aatgtttttg aggtgtgccc taaaccccag atccttgagg gctttctctt accaactttc
     3781 ctttcagagc ctgcttgttg gagattcttc cccagccccc ttcccctttc cctcttgctc
     3841 tgccccacct cgctccaccc agcttgctcc agcccaaaga ttctttattt gtttctcatt
     3901 accgaaggtt gtgagccacc atgtggtttc tgggatttga actcatgacc tccggaggag
     3961 ctgtcatgct cttaaccagc ccatgttgaa gattcttttg ataaatattc acaaaaaata
     4021 aagatgagcc atgagctgtt ggcctcttcg gaagcggaaa ctgagtgatt tgattgaaca
     4081 tccttttatc tttgaccaga ccttggaatg aatgcaatga cctttcccac aggaagaagg
     4141 aggagctctc agtcaaactg taaagaatgc ctcttcagaa tatgctgtca gtgcttggat
     4201 gccatgatgt tcaactttct tagtcgatcc ggcagcaatc acagtgtgag cacactggga
     4261 acctgtcctt gcggccgccg agatctaccg tgtgcttctg tgaagaggct ttgacgtagc
     4321 ccctctttga gctcttacac catgctactg acttctagaa aggctaatta ggtcttcttc
     4381 tacacctaat accctaagtc ttactgactc tcacgggaga agtctctgtg ctacacctga
     4441 gtggtcttat tgataaccct gataccagat caggcaagat aaatccgtca tagcaggcat
     4501 ggctaccctt gctgccacag ggtcacagca catagctcat caccctgtta ttcttcatct
     4561 tgcaatgtgg tatggttttc ctggtgaatg atcagctttt gctgtggtat tctttataca
     4621 tctggactta ttattgaaat caaatgctat agaatcaata gtttatttta tgtctatttt
     4681 tcttgatcgc agagtaatat atattaattg taaaaaattt aagaaacaaa aactatatgt
     4741 aaagaaaaaa ttataatata atacagagat gctgctgaca gttcctatgt gttgtgtttt
     4801 gtatactgag atcatgtgat acgtaggcat acatcttctt gggttttttt gtttttgttt
     4861 tttgttttgt tttgttttgt tttggttttt tgagataggg tttctctgta tagccctggc
     4921 tgtcctggaa ctcactttgc agaccaggct agcctcaaac tcttattcat ttttactgaa
     4981 gtaatttttc tgtcattagt cttcaagagc aaaactttaa tagttatgga gaatattgcc
     5041 agaacagctc aaaactgttt tatttgttgg tccaatttcc cattaattag ttcaataata
     5101 aatatcattt agaaataaaa aaaa
//
