LOCUS       NM_134471               2840 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus kinesin family member 2C (Kif2c), transcript variant
            1, mRNA.
ACCESSION   NM_134471 XM_986203 XM_986238 XM_986272 XM_986300 XM_986329
            XM_986361
VERSION     NM_134471.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Zheng,R., Xu,F.X., Zhou,L., Xu,J., Shen,Y., Hao,K., Wang,X.T. and
            Deng,J.
  TITLE     Ablation of KIF2C in Purkinje cells impairs metabotropic glutamate
            receptor trafficking and motor coordination in male mice
  JOURNAL   J Physiol 601 (17), 3905-3920 (2023)
   PUBMED   37431690
  REMARK    GeneRIF: Ablation of KIF2C in Purkinje cells impairs metabotropic
            glutamate receptor trafficking and motor coordination in male mice.
REFERENCE   3  (bases 1 to 2840)
  AUTHORS   Zheng,R., Du,Y., Wang,X., Liao,T., Zhang,Z., Wang,N., Li,X.,
            Shen,Y., Shi,L., Luo,J., Xia,J., Wang,Z. and Xu,J.
  TITLE     KIF2C regulates synaptic plasticity and cognition in mice through
            dynamic microtubule depolymerization
  JOURNAL   Elife 11, e72483 (2022)
   PUBMED   35138249
  REMARK    GeneRIF: KIF2C regulates synaptic plasticity and cognition in mice
            through dynamic microtubule depolymerization.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2840)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2840)
  AUTHORS   Rondelet,A., Lin,Y.C., Singh,D., Porfetye,A.T., Thakur,H.C.,
            Hecker,A., Brinkert,P., Schmidt,N., Bendre,S., Muller,F., Mazul,L.,
            Widlund,P.O., Bange,T., Hiller,M., Vetter,I.R. and Bird,A.W.
  TITLE     Clathrin's adaptor interaction sites are repurposed to stabilize
            microtubules during mitosis
  JOURNAL   J Cell Biol 219 (2) (2020)
   PUBMED   31932847
  REMARK    GeneRIF: Clathrin's adaptor interaction sites are repurposed to
            stabilize microtubules during mitosis.
REFERENCE   6  (bases 1 to 2840)
  AUTHORS   Vogt,E., Sanhaji,M., Klein,W., Seidel,T., Wordeman,L. and
            Eichenlaub-Ritter,U.
  TITLE     MCAK is present at centromeres, midspindle and chiasmata and
            involved in silencing of the spindle assembly checkpoint in
            mammalian oocytes
  JOURNAL   Mol Hum Reprod 16 (9), 665-684 (2010)
   PUBMED   20406800
  REMARK    GeneRIF: MCAK appears to possess a unique distribution and function
            in oocyte maturation.
REFERENCE   7  (bases 1 to 2840)
  AUTHORS   Parra,M.T., Gomez,R., Viera,A., Llano,E., Pendas,A.M., Rufas,J.S.
            and Suja,J.A.
  TITLE     Sequential assembly of centromeric proteins in male mouse meiosis
  JOURNAL   PLoS Genet 5 (3), e1000417 (2009)
   PUBMED   19283064
  REMARK    GeneRIF: Shugoshin 2 is necessary for the loading of MCAK at the
            inner centromere, but is dispensable for the loading of the outer
            kinetochore proteins BubR1 and CENP-E.
REFERENCE   8  (bases 1 to 2840)
  AUTHORS   Parra,M.T., Gomez,R., Viera,A., Page,J., Calvente,A., Wordeman,L.,
            Rufas,J.S. and Suja,J.A.
  TITLE     A perikinetochoric ring defined by MCAK and Aurora-B as a novel
            centromere domain
  JOURNAL   PLoS Genet 2 (6), e84 (2006)
   PUBMED   16741559
  REMARK    GeneRIF: Possible functions of MCAK at the inner centromere domain
            and at the perikinetochoric ring during both meiotic divisions.
REFERENCE   9  (bases 1 to 2840)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2840)
  AUTHORS   Tchernev,V.T., Barbosa,M.D., Detter,J.C., Patel,T.D., Achey,K.,
            Wakeland,E.K., Gueorguieva,R.V., Yang,M.C., Gossler,A. and
            Kingsmore,S.F.
  TITLE     Genetic mapping of 20 novel expressed sequence tags from
            midgestation mouse embryos suggests chromosomal clustering
  JOURNAL   Genomics 40 (1), 170-174 (1997)
   PUBMED   9070936
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF163926.1, AK150222.1, AK161309.1, CO798410.1 and CK381255.1.
            
            On Apr 23, 2009 this sequence version replaced NM_134471.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088047.1, BC006841.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                CF163926.1         1-70
            71-1772             AK150222.1         2-1703
            1773-2328           AK161309.1         1732-2287
            2329-2828           CO798410.1         16-515
            2829-2840           CK381255.1         322-333
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 53.42 cM"
     gene            1..2840
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /note="kinesin family member 2C"
                     /db_xref="GeneID:73804"
                     /db_xref="MGI:MGI:1921054"
     exon            1..194
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..2299
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinesin-like protein KIF2C; kinesin-like 6; mitotic
                     centromere-associated kinesin"
                     /codon_start=1
                     /product="kinesin-like protein KIF2C isoform 1"
                     /protein_id="NP_608301.3"
                     /db_xref="CCDS:CCDS18531.1"
                     /db_xref="GeneID:73804"
                     /db_xref="MGI:MGI:1921054"
                     /translation="MESLHARLFPGLSINIQRSNGLIHPANISTVNVEKSCVSVEWIE
                     GGTTKGKEIDIDDVAAINPELLQLLPLRPKDSLPLQENVTVPKQKRKSVNSKIPALKE
                     GLRSRSTRMSTVSEVRIPAQENEMEVELPVPTNSRKQFAIPSHPRASCSTVTELPLLM
                     VSEEAEEQAHSTRSTSSANPGNSVRRKSCIVKEMEKMKNKREEKRAQNSELRIKRAQE
                     YDSSFPNWEFARMIKEFRVTMECSPLTVTDPIEEHRICVCVRKRPLNKQELAKKEIDV
                     ISVPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFE
                     GGKATCFAYGQTGSGKTHTMGGDLSGKSQNASKGIYAMASRDVFLLKNQPRYRNLNLE
                     VYVTFFEIYNGKVFDLLNKKAKLRVLEDSRQQVQVVGLQEYLVTCADDVIKMINMGSA
                     CRTSGQTFANSNSSRSHACFQILLRTKGRLHGKFSLVDLAGNERGADTSSADRQTRME
                     GAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGIS
                     SCEYTLNTLRYADRVKELSPHSGPSGEQPVQMETEVMEASSNGTSLTGNEEEELSSQM
                     SSFNEAMTQIRELEERALEELREIIQQGPNWLELSEMTDQPDYDLETFVNKAESALTQ
                     QAKQAKHFSALREVIKALRLAMQLEEQASKQINSKKRHQ"
     exon            195..289
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            290..391
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            392..440
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            441..563
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            564..683
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            684..784
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            785..880
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            881..935
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1098
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1189
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1253
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1345
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1488
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1693
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1804
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1872
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1978
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2083
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2832
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2308..2313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /note="hexamer: AATAAA"
     polyA_site      2329
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /note="major polyA site"
     regulatory      2817..2822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
                     /note="hexamer: AAGAAA"
     polyA_site      2832
                     /gene="Kif2c"
                     /gene_synonym="4930402F02Rik; ESTM5; Knsl6; MCAK"
ORIGIN      
        1 tgacgttgta gggaggctgg cgcgcgggat ttaaactgca gcggtttagg cgttgttaac
       61 acagcgcagt attagcagag tcgtggtttc caagcttctt tcatttgtgt tgcctgttgt
      121 tgttcctgag tccatggagt cgcttcacgc acgcctgttt cctggtctct ccatcaacat
      181 tcaacgcagc aatggcttaa ttcaccccgc caatataagc acagtgaacg tggagaaatc
      241 ctgtgtttcg gtggaatgga tcgaaggagg taccacaaaa ggcaaagaga ttgacattga
      301 cgatgtggct gcaataaacc cagagctcct acagcttctt cccttacgac cgaaggacag
      361 tctgccactg caggaaaacg taacggtccc gaagcaaaaa cgcaaatcag tcaactccaa
      421 aattccagct ctaaaagaag gccttcgaag ccgctccact cgcatgtcca ctgtctcaga
      481 agttcgaatc cctgctcagg agaatgaaat ggaggtggag ctgcctgtgc ctacaaactc
      541 tcgcaagcag tttgccattc ccagccaccc tcgggcctcc tgctccacag tgacagaatt
      601 gccgttgttg atggtcagtg aggaggcaga agagcaagcc cactccaccc gcagtacctc
      661 atctgcaaac cctgggaact cagttcggag gaaatcgtgt atcgtgaagg agatggagaa
      721 aatgaagaac aagcgtgaag agaagagggc ccagaactcg gagctaagaa taaagcgagc
      781 acaggaatat gacagcagct ttccaaactg ggaatttgcc cggatgatca aagaatttcg
      841 ggttactatg gaatgtagcc cgcttactgt gacagaccct atcgaagagc acaggatctg
      901 tgtctgtgtc aggaaacgcc cactgaataa acaagaactg gccaagaaag aaatcgatgt
      961 gatttctgtt cccagcaagt gtctcctcct ggtgcatgaa cccaagttaa aagtggactt
     1021 aacgaagtac ctggagaacc aagctttctg ctttgacttt gcatttgatg agacagcttc
     1081 caatgaagtt gtctacaggt tcacagcaag gccactggta cagacgattt ttgaaggggg
     1141 aaaagcaaca tgctttgcct atggacagac aggcagtggg aagacacata caatgggtgg
     1201 agatctgtct ggtaaaagtc agaacgcatc taaagggatc tacgccatgg cttcccggga
     1261 tgtcttcctt ctgaagaacc agcctcggta ccggaactta aacctggaag tttatgtgac
     1321 attcttcgag atctacaatg ggaaggtgtt tgatctgctc aacaaaaagg ccaagttacg
     1381 tgtgctggag gacagcaggc agcaggtgca ggttgtggga ctgcaggagt acctggtcac
     1441 ctgtgctgat gatgtcatca agatgatcaa catgggcagt gcctgcagga cttctggaca
     1501 aacatttgcc aactccaatt cctcccgctc ccacgcctgc ttccagattc ttcttcgaac
     1561 taaagggaga ttacatggca aattctcttt ggtggatctg gcagggaatg aacgaggggc
     1621 cgacacatct agtgctgacc ggcagactcg catggaggga gcagaaatta acaagtctct
     1681 cctggctctg aaggagtgca tccgggcgct gggacagaac aaggctcaca ccccgttccg
     1741 tgagagcaag ctgacccagg tgctgaggga ctcgttcatt ggggagaact cgaggacctg
     1801 catgatcgcc atgatctcac caggcataag ctcctgtgaa tacaccttaa acacgctgag
     1861 atacgcagac agagtcaagg agctaagccc ccacagcgga cccagtggag agcagccggt
     1921 tcagatggaa acagaagtga tggaagccag ctctaatggg acgtctctga ctggcaatga
     1981 agaggaggag ctgtcttccc agatgtccag ctttaatgaa gccatgactc agatcaggga
     2041 gctggaagag agggccctgg aagagctcag agagatcata cagcaaggac caaactggct
     2101 tgagctctcc gagatgacag atcagccaga ctatgacttg gagacctttg tgaacaaagc
     2161 agaatctgcc ctgacacagc aggctaagca ggcgaagcac ttctcagccc ttcgagaagt
     2221 catcaaggcc ctgcgcctag ccatgcaact ggaagaacag gctagcaaac aaataaacag
     2281 caagaaacgg caccaatgat gacttcaaat aaagatctgt ttgatatcac actagcctct
     2341 tcccctcccc agaaaacttt gggcaccttc tggctttggt taggaactga gttggacagg
     2401 ttgggtaaat ttcaacctca gggcatcatg cccaggaaag ctggggagag gcgatgttct
     2461 tttcctcagt ttggagcggg aagaaggagt tttaatgact gtgcttcctc ttctcctccc
     2521 catggaaggg gggggggatc cctggctcag catccactgc tggtggaaac ctcgctgcga
     2581 ggtgccgctg tgaggtgtgc cgctgtgagg tgcgtgctct ggaagagagg cggagcttgt
     2641 ccgcttcctg ctgcctctgc ctgcagtacc accctcggtc taagcactca tgattttata
     2701 ctttcagaaa aaagttttaa gacttctttg ggttttttgt ttttgttttg aagcctaaga
     2761 aagtcactag ttcttgcctt tatgtattta tatgctgtac ctaacaataa agaaagaaga
     2821 aaaaaaacaa aaaaaaaaaa
//
