LOCUS       NM_001362434            5462 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus kinesin family member 20B (Kif20b), transcript variant
            2, mRNA.
ACCESSION   NM_001362434 XM_006527083
VERSION     NM_001362434.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   McNeely,K.C. and Dwyer,N.D.
  TITLE     Cytokinesis and postabscission midbody remnants are regulated
            during mammalian brain development
  JOURNAL   Proc Natl Acad Sci U S A 117 (17), 9584-9593 (2020)
   PUBMED   32273386
  REMARK    GeneRIF: This study investigated whether polarized furrowing and
            abscission in mouse neural stem cells are regulated differently at
            earlier and later stages and in a cytokinesis mutant, Kif20b.
REFERENCE   3  (bases 1 to 5462)
  AUTHORS   Little,J.N. and Dwyer,N.D.
  TITLE     p53 deletion rescues lethal microcephaly in a mouse model with
            neural stem cell abscission defects
  JOURNAL   Hum Mol Genet 28 (3), 434-447 (2019)
   PUBMED   30304535
REFERENCE   4  (bases 1 to 5462)
  AUTHORS   Geister,K.A., Timms,A.E. and Beier,D.R.
  TITLE     Optimizing Genomic Methods for Mapping and Identification of
            Candidate Variants in ENU Mutagenesis Screens Using Inbred Mice
  JOURNAL   G3 (Bethesda) 8 (2), 401-409 (2018)
   PUBMED   29208648
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5462)
  AUTHORS   McNeely,K.C., Cupp,T.D., Little,J.N., Janisch,K.M., Shrestha,A. and
            Dwyer,N.D.
  TITLE     Mutation of Kinesin-6 Kif20b causes defects in cortical neuron
            polarization and morphogenesis
  JOURNAL   Neural Dev 12 (1), 5 (2017)
   PUBMED   28359322
  REMARK    GeneRIF: These results demonstrate that Kif20b is required
            cell-autonomously for proper morphogenesis of cortical pyramidal
            neurons. Kif20b regulates neuron polarization, and axon and
            dendrite branching, outgrowth, and caliber.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5462)
  AUTHORS   Dwyer,N.D., Manning,D.K., Moran,J.L., Mudbhary,R., Fleming,M.S.,
            Favero,C.B., Vock,V.M., O'Leary,D.D., Walsh,C.A. and Beier,D.R.
  TITLE     A forward genetic screen with a thalamocortical axon reporter mouse
            yields novel neurodevelopment mutants and a distinct emx2 mutant
            phenotype
  JOURNAL   Neural Dev 6, 3 (2011)
   PUBMED   21214893
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5462)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   8  (bases 1 to 5462)
  AUTHORS   Nobrega,M.A., Zhu,Y., Plajzer-Frick,I., Afzal,V. and Rubin,E.M.
  TITLE     Megabase deletions of gene deserts result in viable mice
  JOURNAL   Nature 431 (7011), 988-993 (2004)
   PUBMED   15496924
REFERENCE   9  (bases 1 to 5462)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5462)
  AUTHORS   Miki,H., Setou,M., Kaneshiro,K. and Hirokawa,N.
  TITLE     All kinesin superfamily protein, KIF, genes in mouse and human
  JOURNAL   Proc Natl Acad Sci U S A 98 (13), 7004-7011 (2001)
   PUBMED   11416179
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113244.19 and BC172148.1.
            
            On Apr 17, 2018 this sequence version replaced XM_006527083.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC151061.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AC113244.19        142374-142407
            35-5304             BC172148.1         1-5270
            5305-5462           AC113244.19        195609-195766
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 29.79 cM"
     gene            1..5462
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /note="kinesin family member 20B"
                     /db_xref="GeneID:240641"
                     /db_xref="MGI:MGI:2444576"
     exon            1..76
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            77..224
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..5282
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     M-phase phosphoprotein 1; kinesin-like protein KIF20B;
                     kinesin-related motor interacting with PIN1; N-6 kinesin"
                     /codon_start=1
                     /product="kinesin-like protein KIF20B isoform 2"
                     /protein_id="NP_001349363.1"
                     /db_xref="CCDS:CCDS89382.1"
                     /db_xref="GeneID:240641"
                     /db_xref="MGI:MGI:2444576"
                     /translation="MESHLNPDGVPRPSYVFSADPIARPLEINFDGVKLDLSHEFSLV
                     ASNPAANSLGSKNYLQVCLRIRPFTQSEKEHEAEGCVQVLDSQSVLLKDPQSILGHLS
                     EKSSGQVAQKFSFSKVFGPETSQKEFFLGCIMQPVKDLLEGHSRLIFTYGLTNSGKTY
                     TFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLKLSSDQEKEESANKNT
                     LLRQIKEVTIHNDSYDVLCGHLTNSLTIPEFEESVNSCDQSSLNVDNIKYSVWVSFFE
                     IYNESIYDLFVPVSSKFQKRKMLRLSQDIKGYSFIKDLQWVQVSDSKEAYRLLKLGVK
                     HQSVAFTKLNNASSRSHSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQNE
                     GERLREAGNINTSLLTLGKCINVLKNSEKSKVQHVPFRESKLTHYFQSFFTGKGKICM
                     IINISQSCSAYDETLNVLKFSTTAQRVYVPDTLSSSQEKSFASNKSLQDVSLDSNLDN
                     KILNVKRKTVSWENSLEDVLENEDLVEDLEENEETQNMETELTDEDSDKSLEECRVST
                     CHKKNKELLDLIEKLNKRLINENKEKLTLELKIREEVTQEFTQYWSQREADFKETLLH
                     EREILEENAERRLAIFKDLVGKCDSQDEPTNRICDIELETEEAIACLQLKFNQVKAEL
                     AETKEELIKAQEELKNRESNSLVQALKTSSKVDTSLTSNKSTCNETSEMPKNSRAQTH
                     SERKRLNEDGLQLGEPPAKKGLILVSPPITEEQNKMGEMQQSVSEVVEGNRVLKEKNE
                     ELKRLLTIGENELRNEKEEKAELNKQVVSLQQQLRFFEEKNSSLRADVEQIQASYNSA
                     VAELQTQKAVNQEQRDRILKLSQEMETAARSIESNVSQIKQMQTKIDELRSLDSPSHI
                     SKIDLLNLQDLSSGAKGDNCLNTSQQLPGGDFSSTWVKEYHTQEISRENSFHASIEAI
                     WEECKEIVKASSKKSHQIQGLEEQIEKLQVEVKGYREENSDLRAQESQGKNRDHQLKE
                     KESLIQQLREELQEKSVSLRVQVQLVAEREQALSELSQDVTCYKAKIKDLEVIVETQK
                     DECKRLVELEQSILEKESAILKLEANLKECEAKHQDHIRTNDLSAKEVKFREEVTRLA
                     NNLHDTKQLLQSKEEENEISRQETEKLKEELAANSILTQNLKADLQKKEEDCAELKEK
                     FIDAKKQIEQVQREVSVMRDEEKLLRIKINELEKKKNQYSQDLDMKQRTIQQLKEQLS
                     NQKMEEAVQQYEKVCKDLSVKEKLVEDMRLTLVEQEQTQAEQDRVLEAKSEEADWLAT
                     ELDKWKEKFKDLETRSNQRLNTGTMDDLDVLTRKFSKLQDELQESEEKYKADRKKWLE
                     EKAVLTTQAKEAENVRNREMRKYADDRERCLKLQNEVETLTAQLAEKNSELQKWREER
                     DQLVTAVETQMKALLSSCKHKDEEIQELRKAAAKSTGTENQTMNPKPEYNDSVDLGGV
                     ETEPQSTSLEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKMAVK
                     HPGCPTPVTIKIPKARKRKSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSPVKKEQ
                     KVSVGPSSKKTYSLRSQASTVSANIASKKREGTLQKFGDFLQHSPTILQSKAKKIIET
                     MSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVILMEQKVKETDHQILKR
                     RLRTRTAK"
     exon            225..311
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            312..428
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            429..567
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            568..752
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            753..789
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            790..1014
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1126
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1198
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1345
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1505
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1793
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1933
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2072
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2181
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2216
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2358
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..3592
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3593..3731
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3732..3851
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3852..3912
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3913..4038
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4039..4166
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4167..4322
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4323..4499
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4500..4610
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4611..4808
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4809..4910
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4911..5061
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5062..5204
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5205..5462
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5429..5434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /note="hexamer: AATAAA"
     polyA_site      5447
                     /gene="Kif20b"
                     /gene_synonym="33cex; C330014J10Rik; magoo; Mphosph1;
                     MPP1"
                     /note="major polyA site"
ORIGIN      
        1 gccattgttt gaatttgaaa accgggagga agtggaattc tgcaggtggg gaggcgtgca
       61 gggaccagcg attgcagatg gaatctcact tgaatccaga tggagtgcct agaccgtctt
      121 acgtttttag tgctgaccca attgcaaggc ctttggaaat caattttgat ggtgttaagc
      181 ttgatctctc tcatgagttt tccttagtcg cctcaaaccc tgcggcaaac agtttgggat
      241 ctaaaaacta tcttcaagtt tgtcttcgaa taagaccatt tacacagtca gaaaaagaac
      301 atgaggctga gggctgtgtg caagttttgg attcacagtc tgttctgctg aaagaccctc
      361 aaagcatcct tggccactta agtgagaaga gctctggaca ggtggcacag aaattcagtt
      421 tttctaaggt ttttggacct gaaacttcac agaaagagtt ctttctgggt tgcattatgc
      481 agccagtaaa ggacctctta gaaggacaca gtcgactgat cttcacatat ggtctgacca
      541 attctggaaa aacatataca tttcaaggca cagaagaaaa tattggtatt ttgcctagaa
      601 cactgaatgt attgtttgat agtcttcagg aaagactata tacgaagatg agcttcaaac
      661 cacacagatg tagagaatac ttaaaattat catctgatca agagaaggag gaaagtgcta
      721 acaaaaatac attgctgcga caaattaagg aggttaccat acataatgac agttatgacg
      781 ttctttgtgg acacttaaca aactctttga ctatcccaga atttgaagaa tccgtgaaca
      841 gttgtgacca gtccagtctg aatgtggata atataaagta ttctgtgtgg gtttctttct
      901 ttgaaattta caatgaaagt atttacgatt tgtttgttcc tgtgtcctct aagtttcaaa
      961 agagaaaaat gttacgactt tcccaagata taaagggcta ttcttttata aaagatctgc
     1021 agtgggtcca ggtatctgac tctaaagaag catatagact cttaaaacta ggagtgaagc
     1081 atcagagcgt tgctttcacc aaactgaaca atgcttctag tagaagtcac agcatattca
     1141 ctattagaat attacagata gaagattctg aaatacctcg tgtaacacga gttagtgaat
     1201 tgtctctatg tgatcttgct ggttcagaac gaagcatgaa gacacaaaac gaaggtgaaa
     1261 ggttaagaga ggctgggaat atcaacactt ctttattgac tctgggaaag tgtatcaatg
     1321 ttttgaagaa cagtgaaaag tcaaaggttc agcatgtgcc tttccgagaa agtaaactga
     1381 cccactattt ccaaagtttt ttcactggaa aagggaaaat atgcatgatc atcaatatta
     1441 gtcagtcttg ctctgcctat gatgagacac tcaatgtcct gaagttctca accactgcgc
     1501 aaagggtcta tgttccggat actttaagtt cttctcaaga gaaatctttt gcatctaaca
     1561 aatctttaca agatgtatca ttagacagta atttggacaa taaaatacta aatgtaaaaa
     1621 gaaaaactgt ttcatgggaa aatagtctag aagatgtgct tgaaaatgaa gatttggttg
     1681 aggacttaga agaaaatgaa gaaacacaaa acatggaaac ggaacttaca gatgaagata
     1741 gtgataagtc attagaggaa tgcagagttt ccacctgcca caaaaagaac aaggaactgt
     1801 tggatttaat agaaaagttg aataaaagac taataaatga aaataaagaa aaattaacat
     1861 tggaacttaa aatccgagaa gaagttacac aggaattcac tcagtattgg agccaacggg
     1921 aagccgattt taaggagaca cttcttcatg aacgagagat attagaagaa aacgctgaac
     1981 gtcgcttggc aatcttcaaa gatttggttg gaaaatgtga cagtcaagat gaaccaacaa
     2041 atagaatttg tgacatagaa cttgaaactg aagaagctat tgcctgtttg cagctaaagt
     2101 ttaatcaagt taaagcagaa ttagctgaaa ctaaagaaga attaattaaa gcccaagaag
     2161 agctaaaaaa cagagagagc aactctttgg ttcaggcact caagacatca agtaaggttg
     2221 atacatcttt aaccagcaat aagtcaactt gtaatgaaac aagtgaaatg ccaaagaaca
     2281 gtagagccca gacacattca gaaaggaaaa gattaaatga agatggactt cagcttggtg
     2341 agccaccagc aaagaaaggg cttatactcg ttagtccacc catcaccgaa gagcaaaaca
     2401 aaatgggaga aatgcaacag tctgtttcag aagttgtgga aggcaataga gttttgaagg
     2461 aaaagaatga agagctaaaa agacttttaa ctattggtga gaatgaactc agaaatgaaa
     2521 aggaggaaaa agcagaatta aataaacagg ttgtgagttt gcagcagcaa cttcgtttct
     2581 ttgaagaaaa gaactcatcc ctgagggcag atgtagagca aatccaggcc agttacaaca
     2641 gtgcagttgc cgagctgcaa acacagaaag ccgtgaacca ggagcagagg gacaggatcc
     2701 tgaaattatc acaggagatg gagactgctg caagaagtat cgagagcaat gtttcacaga
     2761 taaaacaaat gcaaacaaaa atagatgaat tgcggtcact tgattcacct tctcatattt
     2821 caaaaataga tttacttaat ctccaggatc tatcaagtgg tgctaaaggg gataattgtc
     2881 tgaacacatc acagcagctt ccaggtggtg acttctcaag tacgtgggtt aaagaatatc
     2941 acactcagga gatcagcagg gaaaattcct tccacgcgag tattgaagcc atctgggaag
     3001 aatgtaaaga gattgtgaag gcttcctcca aaaagtctca tcagattcag ggactagaag
     3061 aacaaattga aaaattacag gtggaagtaa aaggatacag ggaggagaac agtgacctcc
     3121 gagcacagga gagccagggt aagaatagag atcaccagct gaaggagaaa gaaagtctca
     3181 tccagcagct cagagaagaa ctgcaagaaa aaagtgtcag tctccgtgtt caagtacagc
     3241 ttgtagcgga aagagagcaa gctctttcag agctttccca ggatgttacc tgctacaagg
     3301 caaaaataaa ggaccttgag gtgatagtag agacccagaa agatgagtgt aagcgcttag
     3361 ttgagttaga gcaaagcatt ttagagaagg aatctgccat cttaaaactg gaagcaaatc
     3421 taaaggaatg tgaagcaaag catcaggatc acatcagaac caacgatcta agtgcaaagg
     3481 aagtcaagtt cagagaagaa gtcacacggt tggcgaataa tttgcatgat acgaaacagt
     3541 tacttcagtc aaaggaagaa gaaaatgaaa ttagcagaca agagacagaa aagttgaaag
     3601 aggagcttgc tgcaaactct attcttactc agaatctcaa agcagacctt cagaagaagg
     3661 aagaagattg tgctgagctg aaagagaagt tcattgatgc caaaaagcag attgagcagg
     3721 tacagaggga ggtatctgta atgcgtgatg aagagaaatt attgaggatt aaaattaatg
     3781 aactggagaa aaagaaaaac cagtattctc aggacttaga tatgaaacaa cgaaccattc
     3841 agcaacttaa ggagcagtta agtaatcaga aaatggaaga agctgtacaa cagtatgaga
     3901 aagtgtgcaa agatctcagt gttaaggaga agctggttga agacatgcga ctgacgctag
     3961 tggagcagga gcagactcag gcagagcagg accgggtgct cgaggccaag tcagaggaag
     4021 cagactggct agccacagaa ttggataaat ggaaagaaaa attcaaagat ctggaaacca
     4081 gaagtaatca gaggttaaac acaggaacca tggatgacct agacgtgctt accaggaagt
     4141 tcagcaagct tcaggacgag ctgcaggaat ctgaagagaa atataaagct gatagaaaga
     4201 agtggttaga agaaaaagct gtccttacca ctcaagcgaa agaagcagaa aatgtacgaa
     4261 acagggagat gagaaaatat gctgacgaca gggagcgctg tttaaagctg cagaatgaag
     4321 tggaaacact gacagcacag ctggcagaga aaaatagtga gcttcagaag tggagagaag
     4381 agcgtgacca gctggtcaca gctgtagaga cacagatgaa ggctctgctc tccagctgca
     4441 agcacaagga tgaagaaatc caggaactaa gaaaggctgc agcaaagagc accgggacag
     4501 aaaaccaaac catgaatccc aagcctgaat ataatgattc cgttgatctt ggtggagttg
     4561 aaactgaacc tcagtcaaca agtttggaaa tttctaggaa cacagcagag gacggatctg
     4621 tagttcttga ctcttgtgaa gtgtcaacag aaaatgtaca aagtactcga tttccaaaac
     4681 ctgaattgga gattcagttt acacctttgc agccaaacaa aatggcagtg aagcaccctg
     4741 gttgtcccac accagtcaca attaagatcc ccaaggcacg gaagaggaag agtggtgaag
     4801 tggaggagga cttggtgaaa tgtgaaaata agaagaattc aacccccaga agtaatgtga
     4861 agtttcccgt ttcagagcat agaaattccc ctgtcaaaaa ggaacaaaag gtttccgttg
     4921 gtccatcatc taagaaaact tactccttac ggagccaagc atctacagtt agtgcaaaca
     4981 tagcctctaa aaaaagagag ggaacactac agaaatttgg agacttcttg cagcattctc
     5041 caacaattct tcaatcaaaa gcaaaaaaga taattgaaac aatgagttct ccaaagttat
     5101 caacggtaga agtaagtaaa gaaaatgtgt cccagccaaa aaaagccaaa cggaaattat
     5161 acagaaatga aatttcatct cccatcaaca tatctggcca agtgatttta atggaacaga
     5221 aagtaaaaga aactgatcat cagattctca aacgacggct tcgaacacgc acagccaaat
     5281 aaattgcatt gggagactgt ttcagtgtaa agtttatatt cattgtcatt gtaaattgta
     5341 agttctaaaa tttacttgta tatatatata tattatgata tattaaatac ctctgtatag
     5401 attggtatct tgtatataat tttaattcaa taaatatatc agtaatatac cggtgtactt
     5461 ta
//
