LOCUS       NM_173022               3130 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus KICSTOR subunit 2 (Kics2), transcript variant 1, mRNA.
ACCESSION   NM_173022
VERSION     NM_173022.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124992.14, AK087110.1 and BC065058.1.
            
            On Jan 13, 2018 this sequence version replaced NM_173022.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065058.1, AK169891.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AC124992.14        22423-22442         c
            21-1147             AK087110.1         2-1128
            1148-1764           BC065058.1         1141-1757
            1765-1765           AC124992.14        10872-10872         c
            1766-1885           BC065058.1         1759-1878
            1886-1886           AC124992.14        10751-10751         c
            1887-2742           BC065058.1         1880-2735
            2743-2743           AC124992.14        9894-9894           c
            2744-3124           BC065058.1         2737-3117
            3125-3130           AC124992.14        9507-9512           c
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 69.94 cM"
     gene            1..3130
                     /gene="Kics2"
                     /gene_synonym="E030027L10"
                     /note="KICSTOR subunit 2"
                     /db_xref="GeneID:270802"
                     /db_xref="MGI:MGI:2670984"
     exon            1..281
                     /gene="Kics2"
                     /gene_synonym="E030027L10"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..1384
                     /gene="Kics2"
                     /gene_synonym="E030027L10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0536 protein C12orf66 homolog; KICSTOR complex protein
                     C12orf66 homolog"
                     /codon_start=1
                     /product="KICSTOR subunit 2 isoform 1"
                     /protein_id="NP_766610.2"
                     /db_xref="CCDS:CCDS24213.1"
                     /db_xref="GeneID:270802"
                     /db_xref="MGI:MGI:2670984"
                     /translation="MGESIPLAAPVPVEQAVLETFFSHLGIFSYDKAKDNVEKEREAN
                     KSAGGSWLSLLAALAHLAAAEKVYHSLTYLGQKLGGQSFFSRKDSIRTIYTSLHNELK
                     KVVAGRGAPGGTAPHVEELLPHLSEQLCFFVQARMEIADFYEKMYALSTQKFINTEEL
                     VSTLDTILRKYSSRFHHPILSPLESSFQLEVGVLSHLLKAQAQISEWKFLPSLVTLHN
                     AHTKLQSWGQTFEKQRETKKHLFGGQSQKAVQPPHLFLWLMKLKTMLLAKFSFYFHEA
                     LSRQTTASEMKALTAKANPDLFGKISSFIRKYDAANVSLIFDNRGSESFQGHGYHHPH
                     SYREAPKGVDQYPAVVSLPSDRPVMHWPNVIMIMTDRASDLNSLEKVVHFYDDKVQST
                     YFLTRPEPHFTIVVIFESKKSERDSHFISFLNELSLALKNPKVFASLKPGSKG"
     exon            282..567
                     /gene="Kics2"
                     /gene_synonym="E030027L10"
                     /inference="alignment:Splign:2.1.0"
     exon            568..3130
                     /gene="Kics2"
                     /gene_synonym="E030027L10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3101..3106
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kics2"
                     /gene_synonym="E030027L10"
                     /note="hexamer: AATAAA"
     polyA_site      3128
                     /gene="Kics2"
                     /gene_synonym="E030027L10"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtgcga gcggagccac gtgggtggag ctggcgcgca ggtcgcatgg gggagtctat
       61 cccgctagcc gccccggtgc cggtggaaca ggcggtgctg gagacgttct tctctcacct
      121 gggtatcttc tcttacgaca aggccaagga caatgtggag aaggaacgag aggccaacaa
      181 gagcgcgggg ggcagctggc tgtcgctgct ggcggccttg gcgcacctgg ccgcggctga
      241 gaaggtctat cacagcctca cctacctggg gcagaaacta gggggccagt cattcttcag
      301 caggaaagac tccatccgca ccatctacac ttccctgcat aacgaactga agaaagtggt
      361 ggccggccgc ggcgccccgg gcggaacggc tcctcacgtg gaggagcttc tcccccacct
      421 gtcagagcag ctctgtttct ttgttcaggc tcggatggag atcgcagact tctacgagaa
      481 gatgtacgcc ctcagcacac agaagttcat caacacagaa gagctggtca gcaccttgga
      541 taccatcctc aggaagtaca gctccagatt tcaccacccc atcctcagcc ctctggagag
      601 cagtttccag ctggaagtgg gcgtgctgag tcatctcctc aaggcgcagg cgcagatctc
      661 ggagtggaag ttcctcccgt ccctggttac cttgcacaac gctcacacga agcttcagag
      721 ctggggccag acctttgaga agcagcggga gaccaagaag cacctgttcg gaggacagtc
      781 tcagaaggcc gtgcagccgc cacacctttt cctctggctg atgaaactca agaccatgct
      841 cttggccaag ttcagctttt acttccacga ggcgctgagc cgacagacga ccgcgtccga
      901 aatgaaagcg ctgacggcca aggccaaccc ggacctcttt ggaaagatct ccagttttat
      961 caggaaatac gacgctgcca acgtctcctt aatttttgac aaccggggct cagagagctt
     1021 tcagggtcat ggctaccacc acccacattc ctaccgagag gcccccaagg gagtggacca
     1081 gtacccagcc gtggtgtctc tgcccagtga caggcccgtc atgcactggc ccaatgttat
     1141 catgatcatg acagaccgag catctgacct gaacagcttg gagaaagtgg tccacttcta
     1201 cgacgacaaa gtccagagca cctacttcct gacccgcccg gaaccccact tcaccatcgt
     1261 cgtcattttt gagtcaaaga aatctgagag agactcccat tttatttcct tcctcaacga
     1321 gctctcgctt gcccttaaga accccaaagt gtttgcaagt ctgaagcctg gatccaaagg
     1381 ttagcaggcg cttgcccaag tttttccaag attggataca ttgttcttag ttgagtagtt
     1441 tgtgatttga atttatatta gctcacgctt atttctgggc agagctaaag acagaacctc
     1501 cctaatcatg ttgcacagtt cataaggacg gagtctccaa ggagtcgtct ggaagagcca
     1561 tggtgactga accaccgtgg tacagcgaag gcgaatcagc attttctgat ttccctgtat
     1621 ttcatgtcaa ttgaatattt gttggtttct catttatctt tccagatttt ttttgttgtt
     1681 gctattctga ccattgtgcc ccagtgtgct tcctagcatc tctctctctc tctctctctc
     1741 tctctctctc tctctctctc tctccctccc tccctccctc cctccttccg tctccccccc
     1801 accccagcca cagggagaga tgggattgtt ttcagtttca caaacactag tattaatagt
     1861 tttacaaaat gatctaatta gtattatact gtaattagaa tcttggtgag aaatttctgt
     1921 caggaggtga agtgggagat gacaattgat actggtcctt acagcagtac aaccatccac
     1981 aggtcctttt caggtgaccc tcagctccat ccgcatcagt ctttaccttt aaagggtttg
     2041 gtagtcacct gtcccaagtc agcccaagcc atggttcagt gtggaaagca ctcagccatg
     2101 tgctttaacc aagtgtttta gtttctcaca cattgcagtt catttttata aaatcaaaat
     2161 gacggactta atactttgtg cttgctccat agtcagggca tccggtgagg ccccagcacc
     2221 tctgggcgtg gtctcttgcc ttctggagat gattgacgtg catgcagaag cagcttctcg
     2281 gcactgcttc tcggcactgg ctgctctggc ctcctgcgag ttcacagggg agcggatgga
     2341 gagagaccac ggaagcattg agctgttact gctgcgtttg tcccctggag ctcctgtaaa
     2401 cgttagagtc agatcaaggc tttcctcggg cttgtatgga gccactgtct gatggggtgt
     2461 ccacagagca tgaggctcat gcagggccgg ggagcccctc ccaggcctcc tggtcaagtg
     2521 gggtccagct gtacaagggt tcactggcca attatctgca tctccccact ttcacacaaa
     2581 cttaagccag ttgtctttaa acaggtgata gtgattgact ctgcattttc tccaggctct
     2641 atacaaatag gaacaaatgt tgaccttaca ttgaaaaatt ggaaacactt ggaattaaaa
     2701 accaaaccat ttttcttctt tttgtgtttt gctgagaaac tgaaaaaata atccttggta
     2761 tgtatttttt tgtttttttt tccaccatta gccttaatga tgtcgttccc agtacctaaa
     2821 agaatatata ggcttcagta aaccatggag ggatgtatat aaaacacata tataaatata
     2881 tattacttaa aatggccaaa tgtatatata tatatatatt tgaattgtat atttattttt
     2941 aaacatattt tgagtaatct aagaggtatt tacaaagaac aggaggcaga tgtgaatatt
     3001 aagaaaatct atggcaacat aaacttaagt ccctatgcac tgccaggatt ggaggaacct
     3061 cgaagattag tactgactct aatcacttct caagctcctc aataaagttt tatatatata
     3121 attacatata
//
