LOCUS       NM_001310524            1590 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus ketohexokinase (Khk), transcript variant 1, mRNA.
ACCESSION   NM_001310524 XM_006503747
VERSION     NM_001310524.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Gasper,W.C., Gardner,S., Ross,A., Oppelt,S.A., Allen,K.N. and
            Tolan,D.R.
  TITLE     Michaelis-like complex of mouse ketohexokinase isoform C
  JOURNAL   Acta Crystallogr D Struct Biol 80 (Pt 6), 377-385 (2024)
   PUBMED   38805243
  REMARK    GeneRIF: Michaelis-like complex of mouse ketohexokinase isoform C.
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Park,S.H., Helsley,R.N., Fadhul,T., Willoughby,J.L.S., Noetzli,L.,
            Tu,H.C., Solheim,M.H., Fujisaka,S., Pan,H., Dreyfuss,J.M., Bons,J.,
            Rose,J., King,C.D., Schilling,B., Lusis,A.J., Pan,C., Gupta,M.,
            Kulkarni,R.N., Fitzgerald,K., Kern,P.A., Divanovic,S., Kahn,C.R.
            and Softic,S.
  TITLE     Fructose induced KHK-C can increase ER stress independent of its
            effect on lipogenesis to drive liver disease in diet-induced and
            genetic models of NAFLD
  JOURNAL   Metabolism 145, 155591 (2023)
   PUBMED   37230214
  REMARK    GeneRIF: Fructose induced KHK-C can increase ER stress independent
            of its effect on lipogenesis to drive liver disease in diet-induced
            and genetic models of NAFLD.
REFERENCE   3  (bases 1 to 1590)
  AUTHORS   Guccini,I., Tang,G., To,T.T., Di Rito,L., Le Blanc,S., Strobel,O.,
            D'Ambrosio,M., Pasquini,E., Bolis,M., Silva,P., Kabakci,H.A.,
            Godbersen,S., Alimonti,A., Schwank,G. and Stoffel,M.
  TITLE     Genetic ablation of ketohexokinase C isoform impairs pancreatic
            cancer development
  JOURNAL   iScience 26 (8), 107368 (2023)
   PUBMED   37559908
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1590)
  AUTHORS   Helsley,R.N., Park,S.H., Vekaria,H.J., Sullivan,P.G., Conroy,L.R.,
            Sun,R.C., Romero,M.D.M., Herrero,L., Bons,J., King,C.D., Rose,J.,
            Meyer,J.G., Schilling,B., Kahn,C.R. and Softic,S.
  TITLE     Ketohexokinase-C regulates global protein acetylation to decrease
            carnitine palmitoyltransferase 1a-mediated fatty acid oxidation
  JOURNAL   J Hepatol 79 (1), 25-42 (2023)
   PUBMED   36822479
  REMARK    GeneRIF: Ketohexokinase-C regulates global protein acetylation to
            decrease carnitine palmitoyltransferase 1a-mediated fatty acid
            oxidation.
REFERENCE   5  (bases 1 to 1590)
  AUTHORS   Andres-Hernando,A., Orlicky,D.J., Cicerchi,C., Kuwabara,M.,
            Garcia,G.E., Nakagawa,T., Sanchez-Lozada,L.G., Johnson,R.J. and
            Lanaspa,M.A.
  TITLE     High Fructose Corn Syrup Accelerates Kidney Disease and Mortality
            in Obese Mice with Metabolic Syndrome
  JOURNAL   Biomolecules 13 (5), 780 (2023)
   PUBMED   37238651
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1590)
  AUTHORS   Hagopian,K., Ramsey,J.J. and Weindruch,R.
  TITLE     Fructose metabolizing enzymes from mouse liver: influence of age
            and caloric restriction
  JOURNAL   Biochim Biophys Acta 1721 (1-3), 37-43 (2005)
   PUBMED   15652177
REFERENCE   7  (bases 1 to 1590)
  AUTHORS   Weninger,S.C., Peters,L.L. and Majzoub,J.A.
  TITLE     Urocortin expression in the Edinger-Westphal nucleus is
            up-regulated by stress and corticotropin-releasing hormone
            deficiency
  JOURNAL   Endocrinology 141 (1), 256-263 (2000)
   PUBMED   10614646
REFERENCE   8  (bases 1 to 1590)
  AUTHORS   Hayward,B.E. and Bonthron,D.T.
  TITLE     Structure and alternative splicing of the ketohexokinase gene
  JOURNAL   Eur J Biochem 257 (1), 85-91 (1998)
   PUBMED   9799106
REFERENCE   9  (bases 1 to 1590)
  AUTHORS   Wightman,P.J., Hayward,B.E. and Bonthron,D.T.
  TITLE     The genes encoding glucokinase regulatory protein and
            ketohexokinase co-localize to mouse chromosome 5
  JOURNAL   Mamm Genome 8 (9), 700-701 (1997)
   PUBMED   9271679
REFERENCE   10 (bases 1 to 1590)
  AUTHORS   Hue,L. and Hers,H.G.
  TITLE     The conversion of (4- 3 H)fructose and of (4- 3 H)glucose to liver
            glycogen in the mouse. An investigation of the glyceraldehyde
            crossroads
  JOURNAL   Eur J Biochem 29 (2), 268-275 (1972)
   PUBMED   4343087
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC109606.11.
            
            On Jul 15, 2015 this sequence version replaced XM_006503747.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.205525.1,
                                           SRR5189682.3563.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-449               AC109606.11        145377-145825
            450-566             AC109606.11        148557-148673
            567-701             AC109606.11        150506-150640
            702-836             AC109606.11        150840-150974
            837-909             AC109606.11        152261-152333
            910-1056            AC109606.11        153339-153485
            1057-1145           AC109606.11        154366-154454
            1146-1303           AC109606.11        154534-154691
            1304-1590           AC109606.11        154780-155066
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 16.9 cM"
     gene            1..1590
                     /gene="Khk"
                     /note="ketohexokinase"
                     /db_xref="GeneID:16548"
                     /db_xref="MGI:MGI:1096353"
     exon            1..449
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     CDS             358..1389
                     /gene="Khk"
                     /EC_number="2.7.1.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hepatic fructokinase"
                     /codon_start=1
                     /product="ketohexokinase isoform 1"
                     /protein_id="NP_001297453.1"
                     /db_xref="CCDS:CCDS80243.1"
                     /db_xref="GeneID:16548"
                     /db_xref="MGI:MGI:1096353"
                     /translation="MEEKQILCVGLVVLDIINVVDKYPEEDTDRRCLSQRWQRGGNAS
                     NSCTVLSLLGARCAFMGSLAPGHVADFVLDDLRQHSVDLRYVVLQTEGSIPTSTVIIN
                     EASGSRTILHAYSFLVADFRQRGVDVSQVTWQSQGDTPCSCCIVNNSNGSRTIILYDT
                     NLPDVSAKDFEKVDLTRFKWIHIEGRNASEQVKMLQRIEEHNAKQPLPQKVRVSVEIE
                     KPREELFQLFSYGEVVFVSKDVAKHLGFQSAVEALRGLYSRVKKGATLVCAWAEEGAD
                     ALGPDGQLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSKGNSMQEALRFGCQVAGKKC
                     GLQGFDGIV"
     exon            450..566
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     exon            567..701
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     exon            702..836
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     exon            837..909
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1056
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1145
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1303
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1590
                     /gene="Khk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1568..1573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Khk"
                     /note="hexamer: AATAAA"
     polyA_site      1588
                     /gene="Khk"
                     /note="major polyA site"
ORIGIN      
        1 gagggagaga acgcttgctt ctgtgctccg cctgcgaagg cgaagtttct gttgccagac
       61 tgtgctagtc cgggtggtcc agggtctgca gcaggcgcag agggatcgga aaggcgatgc
      121 attactagtg cgctttcgct ttgacagctg aggcggaaaa gtgagagggc ctgccattgg
      181 ccgggctagg taacccaccc ttgcaaagca gaaagctccc tgcgggagga gttctgcacg
      241 cagaggagga gccaaggtag ccagtgagaa gttgggacac ggtcctccag tagataagag
      301 gcagagccca gcaggaaccc cctctgcttg cgggtaggaa gcttggggag cagcctcatg
      361 gaagagaagc agatcctgtg cgtggggctg gtggtgctgg acatcatcaa tgtggtggac
      421 aaatacccag aggaagacac ggatcgcagg tgcctgtccc agagatggca gcgtggaggc
      481 aacgcatcca actcctgcac tgtcctttcc ttgcttggag cccgctgtgc cttcatgggc
      541 tctttggccc ctggccacgt tgccgatttt gtcctggatg acctccgcca acattctgtg
      601 gacttacgat atgtggtcct tcagaccgag ggctccatcc ccacttctac agtcatcatc
      661 aacgaggcca gcggcagccg caccattctg cacgcctaca gcttcctggt ggctgacttc
      721 aggcagaggg gcgtggatgt gtctcaagtg acttggcaga gccagggaga taccccttgc
      781 tcttgctgca tcgtcaacaa ctccaatggc tcccgtacca ttatactcta cgacacgaac
      841 ctgccagatg tgtctgctaa ggactttgag aaggtcgatc tgacccggtt caagtggatc
      901 cacattgagg gccggaatgc atcggaacag gtgaagatgc tgcagcggat agaggagcac
      961 aatgccaagc agcctctgcc acagaaggtc cgggtgtcgg tggagataga gaagccccgt
     1021 gaggagctct tccagttgtt tagctatggt gaggtggtgt ttgtcagcaa agatgtggcc
     1081 aagcacctgg ggttccagtc agcagtggag gccctgaggg gcttgtacag tcgagtgaag
     1141 aaaggggcta cgcttgtctg tgcctgggct gaggagggtg ccgatgccct gggccccgat
     1201 ggtcagctgc tccactcaga tgccttccca ccgccccgag tagtagacac tcttggggct
     1261 ggagacacct tcaatgcctc tgtcatcttc agcctctcga agggaaacag catgcaagag
     1321 gccctgagat tcgggtgcca ggtggctggc aagaagtgtg gcttgcaggg gtttgatggc
     1381 attgtgtgag aggcaagcgg caccagctcg atacctcaga ggctggcacc atgcctgcca
     1441 ctgccttctc tacttcctcc agcttagcat ccagctgcca ttccccggca ggtgtgggat
     1501 gtgggacagc ctctgtctgt gtctgcgtct ctgtatacct atctcctctc tgcagatacc
     1561 tggagcaaat aaatcttccc ctgagccagc
//
