LOCUS       NM_133235               2274 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus KH domain containing, RNA binding, signal transduction
            associated 2 (Khdrbs2), mRNA.
ACCESSION   NM_133235
VERSION     NM_133235.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Farini,D., Cesari,E., Weatheritt,R.J., La Sala,G., Naro,C.,
            Pagliarini,V., Bonvissuto,D., Medici,V., Guerra,M., Di Pietro,C.,
            Rizzo,F.R., Musella,A., Carola,V., Centonze,D., Blencowe,B.J.,
            Marazziti,D. and Sette,C.
  TITLE     A Dynamic Splicing Program Ensures Proper Synaptic Connections in
            the Developing Cerebellum
  JOURNAL   Cell Rep 31 (9), 107703 (2020)
   PUBMED   32492419
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Iijima,Y., Tanaka,M., Suzuki,S., Hauser,D., Tanaka,M., Okada,C.,
            Ito,M., Ayukawa,N., Sato,Y., Ohtsuka,M., Scheiffele,P. and
            Iijima,T.
  TITLE     SAM68-Specific Splicing Is Required for Proper Selection of
            Alternative 3' UTR Isoforms in the Nervous System
  JOURNAL   iScience 22, 318-335 (2019)
   PUBMED   31805436
REFERENCE   3  (bases 1 to 2274)
  AUTHORS   Goodwin,L.O., Splinter,E., Davis,T.L., Urban,R., He,H., Braun,R.E.,
            Chesler,E.J., Kumar,V., van Min,M., Ndukum,J., Philip,V.M.,
            Reinholdt,L.G., Svenson,K., White,J.K., Sasner,M., Lutz,C. and
            Murray,S.A.
  TITLE     Large-scale discovery of mouse transgenic integration sites reveals
            frequent structural variation and insertional mutagenesis
  JOURNAL   Genome Res 29 (3), 494-505 (2019)
   PUBMED   30659012
REFERENCE   4  (bases 1 to 2274)
  AUTHORS   Langston,R.G., Rudenko,I.N. and Cookson,M.R.
  TITLE     The function of orthologues of the human Parkinson's disease gene
            LRRK2 across species: implications for disease modelling in
            preclinical research
  JOURNAL   Biochem J 473 (3), 221-232 (2016)
   PUBMED   26811536
  REMARK    Review article
REFERENCE   5  (bases 1 to 2274)
  AUTHORS   Lavalley,N.J., Slone,S.R., Ding,H., West,A.B. and Yacoubian,T.A.
  TITLE     14-3-3 Proteins regulate mutant LRRK2 kinase activity and neurite
            shortening
  JOURNAL   Hum Mol Genet 25 (1), 109-122 (2016)
   PUBMED   26546614
REFERENCE   6  (bases 1 to 2274)
  AUTHORS   Paronetto,M.P., Bianchi,E., Geremia,R. and Sette,C.
  TITLE     Dynamic expression of the RNA-binding protein Sam68 during mouse
            pre-implantation development
  JOURNAL   Gene Expr Patterns 8 (5), 311-322 (2008)
   PUBMED   18321792
REFERENCE   7  (bases 1 to 2274)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2274)
  AUTHORS   Haegebarth,A., Heap,D., Bie,W., Derry,J.J., Richard,S. and
            Tyner,A.L.
  TITLE     The nuclear tyrosine kinase BRK/Sik phosphorylates and inhibits the
            RNA-binding activities of the Sam68-like mammalian proteins SLM-1
            and SLM-2
  JOURNAL   J Biol Chem 279 (52), 54398-54404 (2004)
   PUBMED   15471878
  REMARK    GeneRIF: expression of active BRK/Sik results in increased SLM-1
            and SLM-2 phosphorylation which has functional relevance and leads
            to inhibition of their RNA-binding
REFERENCE   9  (bases 1 to 2274)
  AUTHORS   Robinson,P.A., Brown,S., McGrath,M.J., Coghill,I.D., Gurung,R. and
            Mitchell,C.A.
  TITLE     Skeletal muscle LIM protein 1 regulates integrin-mediated myoblast
            adhesion, spreading, and migration
  JOURNAL   Am J Physiol Cell Physiol 284 (3), C681-C695 (2003)
   PUBMED   12397030
  REMARK    GeneRIF: SLIM1 localizes in an integrin-dependent manner to the
            nucleus and focal adhesions where it functions downstream of
            integrin activation to promote cell spreading and migration.
REFERENCE   10 (bases 1 to 2274)
  AUTHORS   Di Fruscio,M., Chen,T. and Richard,S.
  TITLE     Characterization of Sam68-like mammalian proteins SLM-1 and SLM-2:
            SLM-1 is a Src substrate during mitosis
  JOURNAL   Proc Natl Acad Sci U S A 96 (6), 2710-2715 (1999)
   PUBMED   10077576
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC140214.3, AC125216.3,
            AC122027.1 and AC124771.4.
            
            On Jan 6, 2017 this sequence version replaced NM_133235.2.
            
            Summary: The protein encoded by this gene is similar to the src
            associated in mitosis, 68 kDa protein, which is an RNA-binding
            protein and a substrate for Src-family tyrosine kinases during
            mitosis. This protein has a KH RNA-binding motif and proline-rich
            motifs which may be SH2 and SH3 domain binding sites. A similar rat
            protein is an RNA-binding protein which is tyrosine phosphorylated
            by Src during mitosis. These studies also suggest that the rat
            protein may function as an adaptor protein for Src by binding the
            SH2 and SH3 domains of various other proteins. [provided by RefSeq,
            Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK078160.1, BC132117.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               AC140214.3         89477-89763
            288-415             AC140214.3         160655-160782
            416-532             AC125216.3         185681-185797       c
            533-679             AC125216.3         104246-104392       c
            680-807             AC125216.3         51376-51503         c
            808-1006            AC125216.3         46498-46696         c
            1007-1089           AC122027.1         143123-143205
            1090-1148           AC124771.4         40653-40711
            1149-2274           AC124771.4         54004-55129
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 11.86 cM"
     gene            1..2274
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /note="KH domain containing, RNA binding, signal
                     transduction associated 2"
                     /db_xref="GeneID:170771"
                     /db_xref="MGI:MGI:2159649"
     exon            1..287
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..1246
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /note="Sam68-like mammalian protein 1; LRRK2<R1441G>"
                     /codon_start=1
                     /product="KH domain-containing, RNA-binding, signal
                     transduction-associated protein 2"
                     /protein_id="NP_573498.1"
                     /db_xref="CCDS:CCDS14861.1"
                     /db_xref="GeneID:170771"
                     /db_xref="MGI:MGI:2159649"
                     /translation="MGEEKYLPELMAEKDSLDPSFVHASRLLAEEIEKFQGSDGKKED
                     EEKKYLDVISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSIL
                     GKGSMRDKTKEEELRKSGEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFLV
                     PDYNDEIRQEQLRELSYLNGSEESGRGRGIRGRGIRITPTAPSRGRGGAVPPPPPPGR
                     GVLTPRGTTVTRGALPVPPIARGVPTPRARGTAAVPGYRAPPPPAHDAYEEYGYDDGY
                     GGEYDDQTYEAYDNSYVTPTQSVPEYYDYGHGVNEDAYDSYAPEEWATTRSSLKAPPP
                     RSARGGYREHPYGRY"
     exon            288..415
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     exon            416..532
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     exon            533..679
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     exon            680..807
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     exon            808..1006
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1089
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1148
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..2274
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1968..1973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /note="hexamer: AATAAA"
     polyA_site      1998
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /note="major polyA site"
     regulatory      2253..2258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /note="hexamer: AATAAA"
     polyA_site      2272
                     /gene="Khdrbs2"
                     /gene_synonym="6330586C16Rik; mSLM-1; Slim1; Slm-1; Slm1;
                     Tg(LRRK2*R1441G)135Cjli; TG-RP135"
                     /note="major polyA site"
ORIGIN      
        1 gggcatcctc tccagctgag gccacggccg gagcctggct ggggcgcggg agaggcaggt
       61 accagcgcgg tctgcgcagc cccaggcgct agagctgcgc tctacggtgg aagaacggtt
      121 cccgggggag ccagggacca aggcgtcgga gcgggcggaa attgcgcctg accgggcacg
      181 gtccgaagtc cgcgctatgg gagaagagaa atacttgcct gagctgatgg cagagaagga
      241 tagcctggat ccatcttttg tgcacgcgtc gcgccttctg gcggaagaaa ttgagaaatt
      301 tcaaggttca gatgggaaaa aggaagatga agaaaagaaa tatctcgatg tcatcagcaa
      361 caaaaacata aagctctctg aaagagtatt gattcctgtg aaacagtatc caaagttcaa
      421 ttttgtgggg aaattgcttg gaccaagagg aaactccttg aagaggctac aagaagaaac
      481 gggtgctaaa atgtctatcc tgggcaaagg gtccatgcga gataagacaa aggaagaaga
      541 gctgaggaag agtggggagg ccaagtatgc ccacctgagt gatgagctgc atgtattaat
      601 tgaagtgttt gctccacccg gggaagctta ttcacggatg agtcatgcct tggaagagat
      661 taaaaaattc ctggttcctg actacaatga tgaaattcgt caagagcaac tccgggagtt
      721 gtcttacttg aatggctcag aagagtctgg ccggggccga ggtattagag gcagagggat
      781 cagaataact cccacagctc catcaagggg ccgtggcggt gctgttccac caccaccacc
      841 acctggacga ggtgtgctta cccctcgggg gaccactgtg acccgtggag ctcttccagt
      901 gcccccaata gcaagaggtg tccccacacc tcgagcccgg gggacggcag cagtaccagg
      961 atacagagca cccccacctc cagctcatga tgcttatgaa gaatatgggt atgatgatgg
     1021 ctatgggggt gaatatgatg accagaccta tgaggcttat gataatagct acgtgacccc
     1081 aacacaaagt gtgcctgaat actatgacta cggtcatgga gtaaacgagg atgcctacga
     1141 cagctacgca ccagaagaat gggccacaac tcgctccagc ctgaaggcac caccaccaag
     1201 gtcagccaga gggggataca gggagcaccc ctatggtaga tattgaaggt cctcttcatc
     1261 tgtgacctcc tcaaagacaa ttcatagcct gtggtctcca cataaacagc aacaagacaa
     1321 gtaatagtcc tttttttgtt tgtttgtttt ttctgttcta ggaataactg ctcataattg
     1381 ctcccactat ttcttgtatt cccttatact gttaatgtga cgtggacatt agtattattt
     1441 tacaaaacag actgaaaaaa aaaagacatt ggcaagcata ctttataaac cattctgtag
     1501 gttgatattc tcttgattgt tttttttaat tgttgtgtgt aaaccccttt tctcttttct
     1561 ttttaatttc cttttccttt tccttttgta agaaaaagca tgaatctgtt cgatttttga
     1621 gtctcaacca actagcagaa cctttgttta gggttgttaa aagactgtaa tatgatttcg
     1681 aactagctga taataaagtt gtagataaga tgttttaacc tgtcttttaa tatatgttag
     1741 tcagatggag atgttcaata tgaagttgta aatttaattt ttaaatgctg ttttaatggg
     1801 gttgaaaata agcagctact gtatgtatgt agctaactga atttgttcag tgttttaacc
     1861 tgtatttgtt aaaaaaagaa aaaagaaaaa aacacacatg gagttccatg tgtaagcttc
     1921 tctaaatagg aaccacaatt tgtcaaatat gtttgccata atttgtcaat aaagctgaag
     1981 gcttttgtaa aaactaaatt tggaattact tgttttctaa ctttaaatgc ctgctttatt
     2041 tctttttcaa gagatgccta aaatgttttc tatttaaaaa ttacaaaaat gaacccaagc
     2101 ctgttttaag atatctgtgt aatacttaaa aaaatgtatc aataacttat ggttgttaaa
     2161 taatttaaaa gttaacaaac taaactgtga catgaatcat ggttagtaaa cactaatgta
     2221 taacatgtta atggaagaaa tggatttaga ataataaatg gattttaaac tatc
//
