LOCUS       NM_016679               4470 bp    mRNA    linear   ROD 03-SEP-2024
DEFINITION  Mus musculus kelch-like ECH-associated protein 1 (Keap1),
            transcript variant 1, mRNA.
ACCESSION   NM_016679
VERSION     NM_016679.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Zhang,Y., Xie,J.Z., Jiang,Y.L., Yang,S.J., Wei,H., Yang,Y. and
            Wang,J.Z.
  TITLE     Homocysteine-potentiated Kelch-like ECH-associated protein 1
            promotes senescence of neuroblastoma 2a cells via inhibiting
            ubiquitination of beta-catenin
  JOURNAL   Eur J Neurosci 59 (10), 2732-2747 (2024)
   PUBMED   38501537
  REMARK    GeneRIF: Homocysteine-potentiated Kelch-like ECH-associated protein
            1 promotes senescence of neuroblastoma 2a cells via inhibiting
            ubiquitination of beta-catenin.
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Wakabayashi,N., Yagishita,Y., Joshi,T. and Kensler,T.W.
  TITLE     Dual Deletion of Keap1 and Rbpjkappa Genes in Liver Leads to
            Hepatomegaly and Hypercholesterolemia
  JOURNAL   Int J Mol Sci 25 (9), 4712 (2024)
   PUBMED   38731931
  REMARK    GeneRIF: Dual Deletion of Keap1 and Rbpjkappa Genes in Liver Leads
            to Hepatomegaly and Hypercholesterolemia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4470)
  AUTHORS   Takahashi,J., Suzuki,T., Sato,M., Nitta,S., Yaguchi,N., Muta,T.,
            Tsuchida,K., Suda,H., Morita,M., Hamada,S., Masamune,A.,
            Takahashi,S., Kamei,T. and Yamamoto,M.
  TITLE     Differential squamous cell fates elicited by NRF2 gain of function
            versus KEAP1 loss of function
  JOURNAL   Cell Rep 43 (4), 114104 (2024)
   PUBMED   38602872
REFERENCE   4  (bases 1 to 4470)
  AUTHORS   Dong,W., Li,Q., Lu,X., Lan,J., Qiu,Z., Wang,X., Wang,J., Zheng,X.,
            Chen,S., Zhang,C. and Jin,J.
  TITLE     Ceramide kinase-mediated C1P metabolism attenuates acute liver
            injury by inhibiting the interaction between KEAP1 and NRF2
  JOURNAL   Exp Mol Med 56 (4), 946-958 (2024)
   PUBMED   38556546
  REMARK    GeneRIF: Ceramide kinase-mediated C1P metabolism attenuates acute
            liver injury by inhibiting the interaction between KEAP1 and NRF2.
REFERENCE   5  (bases 1 to 4470)
  AUTHORS   Occhiuto,C.J. and Liby,K.T.
  TITLE     KEAP1-Mutant Lung Cancers Weaken Anti-Tumor Immunity and Promote an
            M2-like Macrophage Phenotype
  JOURNAL   Int J Mol Sci 25 (6), 3510 (2024)
   PUBMED   38542481
  REMARK    GeneRIF: KEAP1-Mutant Lung Cancers Weaken Anti-Tumor Immunity and
            Promote an M2-like Macrophage Phenotype.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4470)
  AUTHORS   Zipper,L.M. and Mulcahy,R.T.
  TITLE     The Keap1 BTB/POZ dimerization function is required to sequester
            Nrf2 in cytoplasm
  JOURNAL   J Biol Chem 277 (39), 36544-36552 (2002)
   PUBMED   12145307
REFERENCE   7  (bases 1 to 4470)
  AUTHORS   Dinkova-Kostova,A.T., Holtzclaw,W.D., Cole,R.N., Itoh,K.,
            Wakabayashi,N., Katoh,Y., Yamamoto,M. and Talalay,P.
  TITLE     Direct evidence that sulfhydryl groups of Keap1 are the sensors
            regulating induction of phase 2 enzymes that protect against
            carcinogens and oxidants
  JOURNAL   Proc Natl Acad Sci U S A 99 (18), 11908-11913 (2002)
   PUBMED   12193649
  REMARK    GeneRIF: evidence that sulfhydryl groups of Keap1 are the sensors
            regulating induction of phase 2 enzymes that protect against
            carcinogens and oxidants
REFERENCE   8  (bases 1 to 4470)
  AUTHORS   Kotilinek,L.A., Bacskai,B., Westerman,M., Kawarabayashi,T.,
            Younkin,L., Hyman,B.T., Younkin,S. and Ashe,K.H.
  TITLE     Reversible memory loss in a mouse transgenic model of Alzheimer's
            disease
  JOURNAL   J Neurosci 22 (15), 6331-6335 (2002)
   PUBMED   12151510
REFERENCE   9  (bases 1 to 4470)
  AUTHORS   Itoh,K., Wakabayashi,N., Katoh,Y., Ishii,T., Igarashi,K.,
            Engel,J.D. and Yamamoto,M.
  TITLE     Keap1 represses nuclear activation of antioxidant responsive
            elements by Nrf2 through binding to the amino-terminal Neh2 domain
  JOURNAL   Genes Dev 13 (1), 76-86 (1999)
   PUBMED   9887101
REFERENCE   10 (bases 1 to 4470)
  AUTHORS   Chu,C.C. and Paul,W.E.
  TITLE     Expressed genes in interleukin-4 treated B cells identified by cDNA
            representational difference analysis
  JOURNAL   Mol Immunol 35 (8), 487-502 (1998)
   PUBMED   9798653
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163637.4, AK076234.1 and BY586021.1.
            
            On Nov 15, 2007 this sequence version replaced NM_016679.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1, 2, 3, and 4 all encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.234071.1, AK076234.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2262              AC163637.4         27843-30104
            2263-4133           AK076234.1         1749-3619
            4134-4470           BY586021.1         63-399
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 7.74 cM"
     gene            1..4470
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /note="kelch-like ECH-associated protein 1"
                     /db_xref="GeneID:50868"
                     /db_xref="MGI:MGI:1858732"
     exon            1..2262
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /inference="alignment:Splign:2.1.0"
     CDS             1624..3498
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /note="NRF2 cytosolic inhibitor; cytosolic inhibitor of
                     Nrf2; ring canal protein"
                     /codon_start=1
                     /product="kelch-like ECH-associated protein 1"
                     /protein_id="NP_057888.1"
                     /db_xref="CCDS:CCDS22897.1"
                     /db_xref="GeneID:50868"
                     /db_xref="MGI:MGI:1858732"
                     /translation="MQPEPKLSGAPRSSQFLPLWSKCPEGAGDAVMYASTECKAEVTP
                     SQDGNRTFSYTLEDHTKQAFGVMNELRLSQQLCDVTLQVKYEDIPAAQFMAHKVVLAS
                     SSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISVGEKCVLHVMNGAV
                     MYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCTELHQRAREYIYMHFGEVAKQE
                     EFFNLSHCQLATLISRDDLNVRCESEVFHACIDWVKYDCPQRRFYVQALLRAVRCHAL
                     TPRFLQTQLQKCEILQADARCKDYLVQIFQELTLHKPTQAVPCRAPKVGRLIYTAGGY
                     FRQSLSYLEAYNPSNGSWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSA
                     LDCYNPMTNQWSPCASMSVPRNRIGVGVIDGHIYAVGGSHGCIHHSSVERYEPERDEW
                     HLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITPMNTIRS
                     GAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMRHHRSALGITVHQGKI
                     YVLGGYDGHTFLDSVECYDPDSDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQNCTC
                     "
     exon            2263..2948
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /inference="alignment:Splign:2.1.0"
     exon            2949..3154
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3331
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /inference="alignment:Splign:2.1.0"
     exon            3332..4470
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4101..4106
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /note="hexamer: AATACA"
     polyA_site      4136
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /note="major polyA site"
     regulatory      4448..4453
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /note="hexamer: AATAAA"
     polyA_site      4469
                     /gene="Keap1"
                     /gene_synonym="INRF2; mKIAA0132"
                     /note="major polyA site"
ORIGIN      
        1 agacccacgc cctgctccct ccgcccggca cctgcaggaa gggctggaac tgcctctgcg
       61 tacccgccgc ccgtttccgc cctcccgctc ctcccacgcg tgccgcccgg gaccccgcag
      121 caccgctgcc ccgatccgag ccctccaccc ccactccggt ccccctcctc tcttcccgga
      181 agcgcggcgc gtggcggccc ggcggcgcgg attggacgcg tggcacctac agagacaccc
      241 gggggggtgg gacggaggtg agcgagcgcc cgcggaggat gcggtgggga gccagctccg
      301 ggagctgccc gcggtcgcgc gtggggccgt gcacgcggtg gggggaagcg cgtgcccttc
      361 tccaagcgcg caccccgccg ccgagcccgt gagccctcgt agggtggtgg ccgcggcgag
      421 tagaggtagg ggtcgcccgc ggccggcgcc ccgggactct tattgtgaca gggtggcgcg
      481 ctgtgcttag tcaccgtgac ccgcgcggcg gaggcggagg cagagcgcgg ccatggcggg
      541 gtgagtgagc cgctccaggc cgcggcccgg gaccaggccc tgcgggctct cccggcgtca
      601 gggctgcgcc tccgagcggt ggggaggccg ctggagcagg cgccgggtac cgggcggccg
      661 ctgcacagcc ccctgcgcaa tgccaggccc gagctccggc agtgtggtca cgcgtgacag
      721 tcgctcacta gctggggccc ctggagcatt tcatcccccc cctccccacg gtgatctaat
      781 agacaaaaca cgcggagtcg cgactccagg ctgagcccag aacctgggga gccagacgca
      841 gaccctctct tgtctcccca catcttcttt gaaagcataa ttcctcccct ggccccaggt
      901 ctccaagggt ctcctgaatc cctccccgtg ggtgttccag atgctgcaca ctctcttgcc
      961 ccaggagctt ggtgttcgct tagtgtttcc tatacagacc ttgctttatt tttaggcctt
     1021 ttctgtcttc tcgctgtgtc tctggagctc agagcagtcc caaatacata aatggcaggc
     1081 tctgtaaatg ttggtgtggt gttgaaagga atctgacatg ttggacgaag gcaaggggag
     1141 ggaaggatgg ctggaacagt gaagaggttg gaaagcgggt gtggagtttt acaggccatt
     1201 gacgatttgg ggtttccatt cttgggctcg gtgaaaggtg ttgggtgatt ctgagcagga
     1261 aaaggaacat gatatgccct gaaggcccgc gagttgagaa gttagtttga atggagccgg
     1321 ctgtgtccag tttacttggc ttggcaaaat ctgcacttag atatcattgc ttagtcttgc
     1381 aaaaaaagaa gcctggctgg acatggtggc acatactgta atcccagcac tcgggaggag
     1441 ccagctttgg ttgcatagtg agttggaagc cagcctaggc tatgtaagac cctgtctcaa
     1501 ataaaataaa ataaagtggc agggtctggt ctaacccagc ctctgttccc agcgctgtgc
     1561 tcttccctcc ctccaggccc ctaacggcta gcagaggaac tgtgtcttgt catcaggaac
     1621 cccatgcagc ccgaacccaa gcttagcggg gctccccgca gcagccagtt cctgcccctg
     1681 tggtcaaagt gccccgaggg ggccggggac gcagtgatgt atgcctccac ggagtgcaag
     1741 gcagaggtga cgccctcgca ggacggtaac cgaaccttca gctacacact agaggatcac
     1801 accaagcagg cttttggcgt catgaacgag cttcgcctga gccagcaact ctgtgacgtg
     1861 accctgcagg tcaaatatga ggacatccca gctgcccaat tcatggctca caaagtggtg
     1921 ctggcctcct ccagcccagt ctttaaagcc atgttcacca acgggcttcg ggagcagggc
     1981 atggaggtgg tgtccatcga aggcatccac cctaaggtca tggaaaggct tattgagttc
     2041 gcctacacgg cctccatctc cgtgggcgag aagtgtgtcc tgcacgtgat gaacggggcg
     2101 gtcatgtacc agattgacag cgtggttcga gcctgcagcg acttcctcgt gcagcagctg
     2161 gaccccagca acgccattgg catcgccaac ttcgcggagc agatcggctg cactgaactg
     2221 caccagcgtg cccgggagta tatctacatg cacttcgggg aggtggccaa gcaggaggag
     2281 ttcttcaacc tgtcacactg ccagctggcc acgctcatca gccgggatga tctgaacgta
     2341 cgctgcgagt ccgaggtgtt ccacgcgtgc atcgactggg tcaaatacga ctgcccgcag
     2401 cggcgcttct acgtgcaggc actgctgcgg gccgtgcgct gccatgcgct cacgccgcgc
     2461 ttcctgcaga cgcagctgca gaagtgtgag atcctgcagg ccgacgcgcg ctgcaaggac
     2521 tacctggtgc agatattcca ggagctcacg ctgcacaagc ccacgcaggc agtgccctgc
     2581 cgcgcgccca aagtgggccg cctcatctac acagcgggcg gttacttccg acagtcgctc
     2641 agctacctgg aggcctacaa cccgagcaat ggctcctggc tgcgcctggc cgatctacag
     2701 gtgccgcgca gtgggctggc aggctgcgtg gtgggtgggc tgctatacgc tgtgggcggc
     2761 cgcaacaact ctccggatgg caacactgac tccagcgccc tggactgcta caaccccatg
     2821 accaaccagt ggtcgccctg tgcctctatg agcgtgccac gcaaccgcat cggggtgggg
     2881 gtcatagatg gccacatcta cgcagtcggg ggttcccacg gctgcatcca ccacagcagc
     2941 gtggagagat atgagccaga gcgggacgag tggcatctag tcgcgccaat gttgacacgg
     3001 aggattggcg tgggcgtggc agtgctcaac cgcttgctgt atgcagtggg gggctttgac
     3061 gggactaacc ggcttaactc cgcagaatgt tactatccag agaggaatga gtggcggatg
     3121 atcacaccga tgaataccat ccggagcggg gccggggtct gcgtgctgca caactgtatc
     3181 tatgcagcag ggggctacga tgggcaggac cagttgaaca gtgtggagcg ctacgacgtg
     3241 gagacagaga cctggacttt cgtagccccc atgaggcatc accgtagtgc gctggggatt
     3301 actgtgcacc agggcaagat ctacgtcctc ggaggctatg atggccacac ttttctggac
     3361 agtgtggaat gctatgaccc ggacagtgat acctggagtg aggtgacccg catgacatct
     3421 ggccgcagcg gggtgggtgt ggccgtcacc atggaaccct gtcggaagca aattgatcaa
     3481 caaaactgta cctgctgaag cacttggaat acctgagcac tgacaacagg acagaaaaac
     3541 agtctgtgta tcactgcttc tctgtactaa agaaaaaaga agaaaacaaa gcataaacag
     3601 aaaacacagg gccgaagagg cggcagaaga agtcatccct tcttccagga agggcgactg
     3661 ggatgccttg taaaggacct tgtggaagac cagaactcaa atccatgggc ccatctgtca
     3721 tagccctgga gcgtccaagt ctgggatggg gtatgggcgg ggcaccctca caggtgagaa
     3781 gcccttgaac tcccaccacc agaagggggg ggacaggcaa agcaggagat cacatgtttt
     3841 tttctttggt tcctgcaact cggtgatcaa ttccagtgga caggggaaga agggacagct
     3901 gaggccaagg ggctgaggct ccctctggaa ctggggccca agggacaagc cggcacagag
     3961 aagcctctgg gctctgagcc ctgaacagtt attttgttaa ataaccctgt aagtttccca
     4021 tgggaataaa gaatggagta ggcacacagg tcttcagagg gcggtcggaa tccctcaggg
     4081 agagacagct cttctattga aatacacgca gatcctgatg gggctggtat ctgaaacccg
     4141 tctattgtct ctgcttgcca ttgtacattc tgctcagaca gggcatcttg cttcttgtgg
     4201 gacacacagt tgtctgtcag tttcagggca ttagaagcca atgacctaac ttctgtgcct
     4261 cctaacttct cctggggcct cctgtgttta gctttatttt gaggcaggga ctcacgtcgt
     4321 ccgggatggc cttcagttca gaccttgaac tgacgctgcc gcctgtccca gcctaccgag
     4381 tgctggggct acatctgtat agcgcaatgc ctggttcctg cttattattt ttgtacccaa
     4441 gcaggaaaat aaaggtttct gggacattgg
//
