LOCUS       NP_001003953            1309 aa            linear   ROD 04-FEB-2025
DEFINITION  lysine-specific demethylase 2B isoform 1 [Mus musculus].
ACCESSION   NP_001003953 XP_144523
VERSION     NP_001003953.1
DBSOURCE    REFSEQ: accession NM_001003953.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1309)
  AUTHORS   Liu,H., Ren,Q., Gong,M., Zuo,F., Li,Q., Huo,D., Yuan,Y., Zhang,Y.,
            Kong,Y., Liu,X., Lu,C. and Wu,X.
  TITLE     Enforced activation of the CREB/KDM2B axis prevents alcohol-induced
            embryonic developmental delay
  JOURNAL   Cell Rep 43 (12), 115075 (2024)
   PUBMED   39661511
REFERENCE   2  (residues 1 to 1309)
  AUTHORS   Zhang,B., Zhao,C., Shen,W., Li,W., Zheng,Y., Kong,X., Wang,J.,
            Wu,X., Zeng,T., Liu,Y. and Zhou,Y.
  TITLE     KDM2B regulates hippocampal morphogenesis by transcriptionally
            silencing Wnt signaling in neural progenitors
  JOURNAL   Nat Commun 14 (1), 6489 (2023)
   PUBMED   37838801
  REMARK    GeneRIF: KDM2B regulates hippocampal morphogenesis by
            transcriptionally silencing Wnt signaling in neural progenitors.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1309)
  AUTHORS   Iwagawa,T., Fukushima,M., Takeuchi,S., Kawamura,Y., Aihara,Y.,
            Ozawa,M., Yakushiji-Kaminatsui,N., Aihara,M., Koseki,H., Suzuki,Y.
            and Watanabe,S.
  TITLE     The histone H3K36 demethylase Fbxl11 plays pivotal roles in the
            development of retinal late-born cell types
  JOURNAL   Genes Cells 28 (7), 482-495 (2023)
   PUBMED   37073980
REFERENCE   4  (residues 1 to 1309)
  AUTHORS   Blackledge,N.P., Farcas,A.M., Kondo,T., King,H.W., McGouran,J.F.,
            Hanssen,L.L.P., Ito,S., Cooper,S., Kondo,K., Koseki,Y.,
            Ishikura,T., Long,H.K., Sheahan,T.W., Brockdorff,N., Kessler,B.M.,
            Koseki,H. and Klose,R.J.
  TITLE     Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2
            recruitment and polycomb domain formation
  JOURNAL   Cell 157 (6), 1445-1459 (2014)
   PUBMED   24856970
  REMARK    GeneRIF: Genetic ablation in embryonic stem cells reveal that
            deletion of PRC1 has genome-wide effects on PRC2 occupancy and
            H3K27me3; recognition of nonmethylated DNA by KDM2B, part of the
            PCGF1/PRC1 variant complex, is important for deposition of
            H2AK119ub1 and recruitment of PRC2 to a subset of CpG island
            targets and that this activity is essential for normal mouse
            development.
REFERENCE   5  (residues 1 to 1309)
  AUTHORS   He,J., Shen,L., Wan,M., Taranova,O., Wu,H. and Zhang,Y.
  TITLE     Kdm2b maintains murine embryonic stem cell status by recruiting
            PRC1 complex to CpG islands of developmental genes
  JOURNAL   Nat Cell Biol 15 (4), 373-384 (2013)
   PUBMED   23502314
REFERENCE   6  (residues 1 to 1309)
  AUTHORS   Fukuda,T., Tokunaga,A., Sakamoto,R. and Yoshida,N.
  TITLE     Fbxl10/Kdm2b deficiency accelerates neural progenitor cell death
            and leads to exencephaly
  JOURNAL   Mol Cell Neurosci 46 (3), 614-624 (2011)
   PUBMED   21220025
  REMARK    GeneRIF: Results suggest that the Fbxl10 gene makes important
            contributions to embryonic neural development by regulating cell
            proliferation and cell death in mice.
REFERENCE   7  (residues 1 to 1309)
  AUTHORS   He,J., Kallin,E.M., Tsukada,Y. and Zhang,Y.
  TITLE     The H3K36 demethylase Jhdm1b/Kdm2b regulates cell proliferation and
            senescence through p15(Ink4b)
  JOURNAL   Nat Struct Mol Biol 15 (11), 1169-1175 (2008)
   PUBMED   18836456
  REMARK    GeneRIF: Results indicate that Jhdm1b is an H3K36 demethylase that
            regulates cell proliferation and senescence through p15(Ink4b).
REFERENCE   8  (residues 1 to 1309)
  AUTHORS   Sanchez,C., Sanchez,I., Demmers,J.A., Rodriguez,P., Strouboulis,J.
            and Vidal,M.
  TITLE     Proteomics analysis of Ring1B/Rnf2 interactors identifies a novel
            complex with the Fbxl10/Jhdm1B histone demethylase and the Bcl6
            interacting corepressor
  JOURNAL   Mol Cell Proteomics 6 (5), 820-834 (2007)
   PUBMED   17296600
  REMARK    GeneRIF: Study using a high throughput proteomics approach
            identified interactors with Rnf2 including Fbxl10/Jhdm1B, casein
            kinase subunits, and the BcoR corepressor
REFERENCE   9  (residues 1 to 1309)
  AUTHORS   Jin,J., Cardozo,T., Lovering,R.C., Elledge,S.J., Pagano,M. and
            Harper,J.W.
  TITLE     Systematic analysis and nomenclature of mammalian F-box proteins
  JOURNAL   Genes Dev 18 (21), 2573-2580 (2004)
   PUBMED   15520277
REFERENCE   10 (residues 1 to 1309)
  AUTHORS   Winston,J.T., Koepp,D.M., Zhu,C., Elledge,S.J. and Harper,J.W.
  TITLE     A family of mammalian F-box proteins
  JOURNAL   Curr Biol 9 (20), 1180-1182 (1999)
   PUBMED   10531037
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK136625.1 and BC065090.1.
            
            On Oct 25, 2004 this sequence version replaced XP_144523.4.
            
            Summary: The protein encoded by this gene is a H3K36-specific
            histone demethylase, which contains an N-terminal jumonji C domain,
            a CxxC zinc finger domain, a plant homeodomain finger, an F-box,
            and eight leucine-rich repeats. Amongst its demonstrated functions,
            this protein plays roles in the suppression of premature cellular
            senescence, leukemia maintenance and development, maintenance of
            mouse embryonic stem cell pluripotency, and induced pluripotent
            stem cell generation. Mice homozygous for a targeted deletion of
            the zinc finger domain display embryonic lethality with development
            ceasing at approximately 7 to 8 days post coitum, demonstrating an
            essential role in early development. A pseudogene of this gene is
            found on chromosome 4. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Oct 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065090.1, AK129479.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 62.63 cM"
     Protein         1..1309
                     /product="lysine-specific demethylase 2B isoform 1"
                     /EC_number="1.14.11.27"
                     /note="F-box and leucine-rich repeat protein 10; F-box
                     protein containing leucine repeats 10; lysine-specific
                     demethylase 2B; F-box protein FBL10; F-box/LRR-repeat
                     protein 10; [Histone-H3]-lysine-36 demethylase 1B; jmjC
                     domain-containing histone demethylation protein 1B"
                     /calculated_mol_wt=149603
     Site            26
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          151..226
                     /region_name="JmjC"
                     /note="A domain family that is part of the cupin
                     metalloenzyme superfamily; smart00558"
                     /db_xref="CDD:214721"
     Region          198..304
                     /region_name="cupin_RmlC-like"
                     /note="RmlC-like cupin superfamily; cl40423"
                     /db_xref="CDD:477354"
     Region          303..>338
                     /region_name="JHD"
                     /note="Jumonji helical domain; pfam17811"
                     /db_xref="CDD:465515"
     Region          378..476
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            447
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            450
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            466
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            470
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          480..546
                     /region_name="CTD_KDM2B"
                     /note="C-terminal domain found in Lysine-specific
                     demethylase 2B; cd21785"
                     /db_xref="CDD:412026"
     Region          <588..624
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          634..695
                     /region_name="PHD_KDM2B"
                     /note="PHD finger found in Lysine-specific demethylase 2B
                     (KDM2B); cd15644"
                     /db_xref="CDD:277114"
     Site            order(634,657..661,665,690)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277114"
     Region          <693..992
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          700..816
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          828..1005
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            924
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            948
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            952
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            991
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Site            1004
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8NHM5; propagated from
                     UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          1033..1077
                     /region_name="F-box_FBXL10"
                     /note="F-box domain found in F-box/LRR-repeat protein 10
                     (FBXL10) and similar proteins; cd22180"
                     /db_xref="CDD:438951"
     Site            order(1038..1039,1041..1043,1045..1046,1048..1050,
                     1052..1053,1056..1057,1059..1061,1065)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438951"
     Region          1050..1072
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1075..1099
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1080..1280
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          1100..1123
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1106..1127
                     /region_name="LRR 1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          1124..1147
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1129..1155
                     /region_name="LRR 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          1148..1187
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1188..1212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1195..1220
                     /region_name="LRR 3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          1213..1242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1221..1250
                     /region_name="LRR 4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          1243..1267
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1251..1275
                     /region_name="LRR 5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     Region          1268..1292
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1276..1309
                     /region_name="LRR 6"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6P1G2.1)"
     CDS             1..1309
                     /gene="Kdm2b"
                     /gene_synonym="Cxxc2; Fbl10; Fbxl10; Jhdm1b; PCCX2"
                     /coded_by="NM_001003953.2:200..4129"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS39259.1"
                     /db_xref="GeneID:30841"
                     /db_xref="MGI:MGI:1354737"
ORIGIN      
        1 meaekdsgrr lraidrqryd enedlsdvee ivsvrgfsle eklrsqlyqg dfvhamegkd
       61 fnyeyvqrea lrvplvfrdk dglgikmpdp dftvrdvkll vgsrrlvdvm dvntqkgtem
      121 smsqfvryye tpeaqrdkly nvislefsht klehlvkrpt vvdlvdwvdn mwpqhlkekq
      181 teatnalaem kypkvkkycl msvkgcftdf hidfggtsvw yhvfrggkif wlipptlhnl
      241 alyeewvlsg kqsdiflgdr vercqrielk qgytffipsg wihavytpvd slvfggnilh
      301 sfnvpmqlri yeiedrtrvq pkfrypfyye mcwyvleryv ycvtqrsylt qeyqrelmli
      361 daprktsvdg fssdswldme eesceqqpqe eeeeeedkee egdgadktpk pptddptspt
      421 stppedqdst gkkpkapair flkrtlsnes eesvkstsmp tddpktptgs patevstkwt
      481 hltefelkgl kalveklesl penkkcvpeg iedpqalleg vknvlkehvd ddptlaitgv
      541 pvvswpkkta knrvvgrpkg klgpasavkl aanrttagar rrrtrcrkce aclrtecgec
      601 hfckdmkkfg gpgrmkqsci mrqciapvlp htavclvcge agkedtveee egkfnlmlme
      661 csicneiihp gclkikeseg vvndelpncw ecpkcnhagk tgkqkrgpgf kyasnlpgsl
      721 lkeqkmnrdn kegqepakrr seceeaprrr sdehpkkvpa dgilrrksdd vhlrrkrkye
      781 kpqelsgrkr asslqtspgs sshlsprppl gsslspwwrs sltyfqqqlk pgkedklfrk
      841 krrswknaed rlslankplr rfkqepeddl peappktres dqsrssspta gpstegaegp
      901 eekkkvkmrr krrlvnkels kelskelnhe iqktestlah esqqpiksep esendepkrp
      961 lshcerphrf skglngtpre lrhslgpglr spprvmsrpp psasppkciq merhvirppp
     1021 ispppdslpl ddgaahvmhr evwmavfsyl shrdlcvcmr vcrtwnrwcc dkrlwtridl
     1081 nrcksitplm lsgiirrqpv sldlswtnis kkqlswlinr lpglrdlvls gcswiavsal
     1141 cssscpllrt ldvqwveglk daqmrdllsp ptdnrpgqmd nrsklrnive lrlaglditd
     1201 vslrliirhm pllsklqlsy cnhindqsin lltavgtttr dsltevnlsd cnkvtdlcls
     1261 ffkrcgnich idlryckqvt kegceqfiae msvsvqfgqv eekllqkls
//
