LOCUS       NM_001371039           18419 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus potassium channel tetramerisation domain containing 16
            (Kctd16), transcript variant 2, mRNA.
ACCESSION   NM_001371039 XM_017317936
VERSION     NM_001371039.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 18419)
  AUTHORS   Ren,Y., Liu,Y., Zheng,S. and Luo,M.
  TITLE     KCTD8 and KCTD12 Facilitate Axonal Expression of GABAB Receptors in
            Habenula Cholinergic Neurons
  JOURNAL   J Neurosci 42 (9), 1648-1665 (2022)
   PUBMED   35017224
REFERENCE   3  (bases 1 to 18419)
  AUTHORS   Fritzius,T., Turecek,R., Seddik,R., Kobayashi,H., Tiao,J.,
            Rem,P.D., Metz,M., Kralikova,M., Bouvier,M., Gassmann,M. and
            Bettler,B.
  TITLE     KCTD Hetero-oligomers Confer Unique Kinetic Properties on
            Hippocampal GABAB Receptor-Induced K+ Currents
  JOURNAL   J Neurosci 37 (5), 1162-1175 (2017)
   PUBMED   28003345
REFERENCE   4  (bases 1 to 18419)
  AUTHORS   Cathomas,F., Sigrist,H., Schmid,L., Seifritz,E., Gassmann,M.,
            Bettler,B. and Pryce,C.R.
  TITLE     Behavioural endophenotypes in mice lacking the auxiliary GABAB
            receptor subunit KCTD16
  JOURNAL   Behav Brain Res 317, 393-400 (2017)
   PUBMED   27717812
  REMARK    GeneRIF: This study demonstrated that the Kctd16(-/-) mice
            exhibited less extinction of auditory fear memory relative to WT
            and Kctd16(+/-) mice, as well as more contextual fear memory
            relative to WT and, in particular, Kctd16(+/-) mice.
REFERENCE   5  (bases 1 to 18419)
  AUTHORS   Metz,M., Gassmann,M., Fakler,B., Schaeren-Wiemers,N. and Bettler,B.
  TITLE     Distribution of the auxiliary GABAB receptor subunits KCTD8, 12,
            12b, and 16 in the mouse brain
  JOURNAL   J Comp Neurol 519 (8), 1435-1454 (2011)
   PUBMED   21452234
  REMARK    GeneRIF: Data suggest that the distinct spatial and temporal
            distribution patterns of KCTD8, 12, 12b, and 16 underlie functional
            differences in native GABA(B) responses.
REFERENCE   6  (bases 1 to 18419)
  AUTHORS   Schwenk,J., Metz,M., Zolles,G., Turecek,R., Fritzius,T., Bildl,W.,
            Tarusawa,E., Kulik,A., Unger,A., Ivankova,K., Seddik,R., Tiao,J.Y.,
            Rajalu,M., Trojanova,J., Rohde,V., Gassmann,M., Schulte,U.,
            Fakler,B. and Bettler,B.
  TITLE     Native GABA(B) receptors are heteromultimers with a family of
            auxiliary subunits
  JOURNAL   Nature 465 (7295), 231-235 (2010)
   PUBMED   20400944
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC098707.2 and AC127249.4.
            
            On Jun 26, 2019 this sequence version replaced XM_017317936.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.470106.1,
                                           SRR9219381.284850.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-649               AC098707.2         175259-175907
            650-1812            AC098707.2         176290-177452
            1813-18419          AC127249.4         96631-113237        c
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18"
     gene            1..18419
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="potassium channel tetramerisation domain containing
                     16"
                     /db_xref="GeneID:383348"
                     /db_xref="MGI:MGI:1914659"
     exon            1..649
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /inference="alignment:Splign:2.1.0"
     exon            650..1812
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /inference="alignment:Splign:2.1.0"
     CDS             981..2264
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /codon_start=1
                     /product="BTB/POZ domain-containing protein KCTD16"
                     /protein_id="NP_001357968.1"
                     /db_xref="CCDS:CCDS50264.1"
                     /db_xref="GeneID:383348"
                     /db_xref="MGI:MGI:1914659"
                     /translation="MALSGNCSRYYPRDQGAAVPNSFPEVIELNVGGQVYFTRHSTLI
                     SIPHSLLWKMFSPKRDTANDLAKDSKGRFFIDRDGFLFRYILDYLRDRQVVLPDHFPE
                     RGRLKREAEYFQLPDLVKLLAPEDVKQSPDEFCHSDFEDASQGSDTRICPPSSLLPHD
                     RKWGFITVGYRGSCTLGREGQADAKFRRVPRILVCGRISLAKEVFGETLNESRDPDRA
                     PERYTSRFYLKFKHLERAFDMLSECGFHMVACNSSVTASFVNQYTEDKIWSSYTEYVF
                     YREPSRWSSSHCDCCCKNGKGDKGESGTSCNDLSTSSCDSQSEASSPQETVICGPVTR
                     QSNIQTLDRPIKKGPVQLIQQSEMRRKSDLLRTLTSGSRESNISSKKKAAKEKLSIEE
                     ELEKCIQDFLKIKIPDRFPERKHPWQSELLRKYHL"
     exon            1813..18419
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2295..2300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="hexamer: AATAAA"
     polyA_site      2334
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="major polyA site"
     polyA_site      2360
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
     regulatory      3923..3928
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="hexamer: AATAAA"
     polyA_site      3960
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
     regulatory      14165..14170
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="hexamer: AATAAA"
     polyA_site      14183
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
     regulatory      15027..15032
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="hexamer: AGTAAA"
     polyA_site      15047
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
     regulatory      16718..16723
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="hexamer: AATAAA"
     polyA_site      16740
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
     regulatory      18398..18403
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
                     /note="hexamer: AATAAA"
     polyA_site      18419
                     /gene="Kctd16"
                     /gene_synonym="2900055J20Rik; 4930434H12Rik;
                     C030017B01Rik; Gm1267"
ORIGIN      
        1 acacaacagc atccgaactg ctcctggcac cgtgggacct ctcagcttcg gagcccaagc
       61 cccgcaggca tctctcgcct tcccggctgc ttttccttcc cacctggcgg ggacatgggc
      121 gtccctttgc ccttgcctgc aggtggggtg gctgctgcaa ccctgggggg cgcatctatc
      181 tgtcgccctg cctctgggct ctctgaatgc ctctaagtcc aggtacctct tgcaactctt
      241 ccaggtgcaa aggatttatc tgttctataa ggtttggatt tcgttttttc tttttttcct
      301 acggaagctt tggttgaata tatatatttt tttcctctgc tccacttctt cgactcagca
      361 tcctctcggt tcaacttttt tcccccccga ttcgttttag ctagagatta gatgtgaatg
      421 catacaccca ttcatacaca catagaaacg tacacctact agagcttaag tgtggaatca
      481 ttgttttccg agagcaccaa tctcttgaca cttttcatcg atacctctgt cctttacctg
      541 tttgggattt gacaagacgt ccaggctcag tgtgtggctg tggtttctga ttgctctgga
      601 aaaggttatt tcttcgcaag aactgagtct ccagcactca ctcgctaaga cagacctctg
      661 agtaactggg catttggcct tcttgcctac agagcctaat ggaagattga atgtagagga
      721 gccgattatt acatcctgtg aagtaagagc tcgctatcag ccagctttta acttctcctc
      781 cagcattatt tcaactgtgg actctcctaa aatctgcttt gttgggagaa taaaaaccaa
      841 aacaaaacca gggtaagaag gggtgattta aaaagaaaag ttagtatctt tatcttacct
      901 cactcgtgga tgcaaagtct agggctgtaa ctcagttgga ttgcaccttt aattccagga
      961 ggctgcagaa gaaagggaca atggctctaa gtgggaactg tagccgttat tatcctcggg
     1021 accagggggc tgctgttccc aactccttcc ctgaagtcat agagctgaat gttggcggcc
     1081 aggtttactt cactcgccat tccacattaa taagcatccc ccattctctc ctgtggaaaa
     1141 tgttctcccc aaagagagac actgctaacg atctagccaa ggactccaag ggaaggtttt
     1201 tcattgacag agatggcttt ctgttccgtt atattctgga ctatctcagg gacaggcagg
     1261 tggtcctgcc tgatcacttt ccagaaagag gaaggctgaa aagagaagct gagtacttcc
     1321 agctccctga cctcgtcaaa ctcctggccc ccgaggatgt caagcaaagc ccggatgagt
     1381 tctgccacag tgacttcgaa gatgcctccc aaggaagcga cacgagaatc tgccccccct
     1441 cttcgctgct tcctcacgac cgcaagtggg gttttattac tgtgggttac aggggatcct
     1501 gtaccttggg cagagagggg caagcagatg ccaagttccg gagagtcccc cggattttgg
     1561 tttgcggaag aatttccttg gcaaaagaag tctttggaga aactttgaat gaaagtagag
     1621 accccgaccg agctccagaa agatacacct ccagatttta tctcaagttt aaacatctgg
     1681 aaagagcttt tgatatgttg tcagagtgtg gattccacat ggtggcctgt aactcctcgg
     1741 ttacagcatc ttttgtcaac cagtatacag aagataagat ctggtcgagc tatactgagt
     1801 acgtcttcta tcgtgagcct tcccggtggt cctcctctca ttgtgactgc tgctgcaaga
     1861 atggcaaggg agacaaagga gagagcggca cctcctgcaa tgacctgtcc acttccagct
     1921 gtgacagcca gtcagaggcc agctctccgc aggagacggt gatctgtggg cctgtaacgc
     1981 gccagagcaa catccagact ctggatcggc ccatcaagaa aggtccggtg cagctgatcc
     2041 aacagtcaga gatgaggcgg aaaagtgacc tgctccggac tctgacgtca ggctccaggg
     2101 agtcgaacat aagcagcaaa aagaaagctg cgaaggaaaa gctctccatc gaggaagagc
     2161 tggagaaatg tatccaggat ttcttgaaga taaaaattcc agatcgcttc cctgagcgaa
     2221 aacatccttg gcagtctgaa cttttacgga agtatcatct atagggggag ggctgtgggt
     2281 agtcgccact ttgaaataaa cctccccaaa ggaagacata tgttaaagga aaaataacaa
     2341 ctaacggtcc acatttgtta gatcacaatg tccattgatg tactactgcc tactttgcct
     2401 agctcacctt aacgtgtaaa tccacagggt agatttcttt ctagatgtgg aaccagaaac
     2461 gagctcttag ttgtcctttg tcttttattt acttggtccc atgtgctgag aatcttaaga
     2521 tacaacaaga accagctacg tgtgagtagc tcacaggctt tgggaatcat tgatcccaaa
     2581 ccaggttttt ttgttttgtt ttgttttgtt ttgtttactc tcatttttct gcctcctccc
     2641 cttgaccaag aatggacagt tgaaggagat ataacccggt ggcttatgtt aagaaattat
     2701 ccttttccct ttccttttgt ttgtttatgg ggttgagggg agaatggcaa atttgtatga
     2761 tttttcacta aaatctctat gtgccaggtt ctattgactt tgtatgcatg agcgtttctg
     2821 acacaagcac agtatatgtc tgtatatatg cacaaagaat gcacacgacc tagggcctgg
     2881 acagcagagg gctaacatct tactatcagc tgcccctaca agagcacttc agacaaccaa
     2941 gcctctgcct atttattaaa accctcctgg gcagatttcc cagcctccct tggcaggcag
     3001 cactttctaa agctgaatag gcccccatca tcaacaaatt ctctttctta ttttgaaata
     3061 aatacccaca ggctcctttg atttattatg ttctttccct acattaggag ctggtgagat
     3121 gagctagtct aaccctgttt gtgtttaaca gacaagcaaa cagtcatatc cacaaacaga
     3181 gtgttgaaag atctcgcact cagccttctc cgttctaatt agaacaatca ccattctcta
     3241 gctgactcag agttttaaac ttgcccacat tttattaaca aggcctttga tataatccag
     3301 gcaaattctc tgcctcccta tgggttgtga agctacgaac aacacccaat gattgaaaat
     3361 gcatccagcc ttccgttccc ttgttttaga ggatttgtgc cccaacatat gtctaaattt
     3421 ctcatacaaa ctttacacta cacctgttta ctgttgcttg cttgtagcca ggtttggata
     3481 actttggaat cctggggttt ggctgtggcc ctactacggt ttagttgtat aatttctaca
     3541 gtgtctgtaa tgacccaagt gggtggctgg aacataaaag ttactaattt gattttttaa
     3601 acttttaaaa aatattcctg acctgtgtag ataccatcca aaagaaactg ttaacactgt
     3661 ctatccatgt gattctgtct tctactaatc ttccagtagt tcttttgttt actgtgctaa
     3721 aagaatgtcc aaagacaact tttaattttc agcattcctc atacatccaa gtggtaactg
     3781 aaaagatgat ttatcactag tgtgtgccaa gaactcctat ttttttgttg tgtgtgtgtc
     3841 tgtgtgtgtg tgtgtttgca tgcatgtgca cacacctgtg cacatgtgtc attgtttgaa
     3901 tgtgtgtatg gttaacaaaa gtaataaagg caatcaaaca atgtctttat agaagaaaaa
     3961 tctaatgacc agttttttct ttctgaaaac tttcattggg gcacatttat tatttaagaa
     4021 tcttctcctt gatttattgg tgcccattag tatatgattg tctttctgaa ttttttataa
     4081 ttcctgatta cacaggtggg taataggata aggcaatatt ttcttgtcct gatacttcaa
     4141 gtaaagttta aaattctatt taagtgaaaa agaaagtttc cttgaggtgc tgagtgacca
     4201 ggaaaattat cctttgtgaa agtgttcttt tgcgtattaa tagtttagca gatagttgat
     4261 agatagcttt gtgtgtaaat cctattaatg catctttctc ctctcaatgg ttcttgatgg
     4321 tacatcaaaa ttactatcat acttattacg gaagatattg tgaagcatca tggatcttaa
     4381 tgggtcaaga agaagctgga tgtgaattgt tggccagctg atcattgact gaatcactta
     4441 aagggctgct agagaagatg agattcgaat aaattggcca aaatccccag gttttaaaat
     4501 tttaacccta atcctaatgg catcgagaca ctcaagaagt ggtctgccat tgacccacag
     4561 cttctactag agtcttcaaa taggaaatca gtgctgctcc caaaagaatt gtagcattca
     4621 ttgtcctgca ggctttgttg ttatgccttt ttcttttcct tcacaggtac tctttgtacc
     4681 cgtgatgagc aagcttagca ctagaaatct gttgaagaaa caacattatc actttcattt
     4741 tggctacttg taaagtggca tctgccccat ggtaaaaagt ttaaacagaa agagaaaaga
     4801 catggtattc attgatggga gtattggcat gttcaggaat acagcctggg tcagtagaag
     4861 gctttgagaa ggcaccaggg atattaaggg tcaggtccct tttggagtag agtcatccag
     4921 cgtgctccct gtagtcatca ccacactgga aatgatcttt ggctaagtga gccatggaaa
     4981 caaaacactc tgctatccct ggcttcagag ccttaacctc agctatgggc agcacagtat
     5041 tacatcaagt tcagttctta ttgtgggttt tgacaggggc tgtacattgt tgatatcaag
     5101 aaatgaatag tgctaggtag tactattcta tccagtacta tgttatctat gctaaaagga
     5161 aaataaaact gttaaaaaga agtatcctac tgaaacccga cacattaaag agccttagtt
     5221 tgggctgcct tctgagaatc ctgtaaggtt aaatccacag aagctagggt ggtcactcct
     5281 taaatggata tgaatctcct gaagtcagag gaacattcct aacatggaca tatttataaa
     5341 gtcctccaac ccactattga gttgtattat atggcaaaca gcagtgggaa gaggaaaata
     5401 atacttattt catttacaca attattctct tccactgctc agaaagaaac aaggcactga
     5461 tggatagtag tgagggatac agtgagatgg cttccaaagt gtgctgtcct atcagtcttc
     5521 cagggagatg catgagtgat gagtttttaa aatggaggaa gagtggaatg acacacatgg
     5581 ttccatagtg gaatgtttat ttcagctaac tcatggtaga gaaacacttg attaacagag
     5641 ggcccacgtg gagccaccca tgtattcatt ggtcttgtct gattcagaag catcattttc
     5701 cacacatggc agatgcctgc ccattcttca ttctcttagc cttccctgaa tatgcttctg
     5761 ggttacgttt ctttccgtag actttccatg gctctgagtg tgctttttac attgctgaga
     5821 agaacatgtt ccaagggatg tgggggagaa atgggggtag actgctagag cagttttaaa
     5881 aattactcta ctacagttcc tacaatggaa tacaaaattt gattctcata acattacttt
     5941 gctatcactt caatctcccc atttgttgtc tagagtagac taacccagaa aagacaggtt
     6001 tttggcattc tcaaccactg gatacattgt cctgaatttt gagtaatgtt ggtcttatgt
     6061 ttgatctttg ctagaattaa taagacattt tctgtgaact tataggataa attctttgtg
     6121 agctctactt ggcataataa ttttctaagg aaaaatgaca ggaattattt tcctcctgat
     6181 gactttttgg cagataaatg tttttgccaa tttgcatgca gagttatagc tcctagtatg
     6241 ggccatttct ctctttaatg tcttggtatt gttagcatct tataatcttg atggcccact
     6301 gttccaattt atttatttat tttatcttat agtctcaaaa tgattcacta gagaaagaat
     6361 cagaataagc agtatgtatc tggaacaaca atggagttag atgtcattga taaggaactg
     6421 tacaatgacg gatagctgca aagaaattag aggaaataaa acatgctcaa atgagtttga
     6481 cttattattg cccccatcat tctggttcca tataaaatgg ttccagttcc ctatgccttt
     6541 tagtatccac agccttgcga ttcagccaca atgtttgttt tgaaactttt attcttctca
     6601 ttttgctatg ggcattggaa aggggaattt ttttccctcc aatgaccact gctgaaaaag
     6661 ttaggaaagc tctagacatc agacacacac taaattaatt tgtggctctt tctgaaaagg
     6721 agattccctt acactgagtt caaaaaatca gattcataaa acttgaagga gggccaagga
     6781 atctgcattt tgatgtagta cctttaatca tttgctggtc taaggaccct acttggagaa
     6841 gttgctattg ggacactaag gggaagtcag catgtggtca gaggccatac cattcactgt
     6901 cttagaaagg ttcacagaat cacattctgg tactaaggta caatacattt tcaaactccc
     6961 ttcatcattt cacctgcctt ccaaccaaag ttgtcttccc aaaagtgggt gtttgtgccc
     7021 tcttttggag tttaaaagcc aacatcacat cattatagcc ttatctctta tcccttgggc
     7081 ccccattcct caatcagaaa gcctttctca tattagcttt accccaggtg gagaggggac
     7141 atatctgagt gtggtctatt ctaatgatac ccaacaggga atgaaagtaa tggtttttct
     7201 cagggccaga tgttcatttc attgctcagt ctgggtcaga ggagtgagaa cccaatccac
     7261 agtttaatga atctgttgcc ccaaagatgg ttccattggt gatgtacaga taccagccaa
     7321 gccaatgtgg aatagtgagt aaattcagct gtggataatg gagattctga accatagcat
     7381 taatcataag tgtaaaacaa aaacacatgt taagaataag cataagagaa aggacagaga
     7441 aaatttaaaa tggtgatagc ccaattgtaa ggacataaag tgatgtgcta taatgtttcc
     7501 cagtctgtct cctgaagaga tgaccatgcc ataatctctg tctgtctacg tctttgtctc
     7561 tctcttcaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca
     7621 cacaccaagg ctggaaggtt atatgattcc cccagttgac ctagcatata acagaagaat
     7681 tattggctag ataaaaaaaa tggccacatg gtataagaag tagaaaaact tgtcataagc
     7741 attgatggat tttgaaataa atacaaaagg aagcaaggaa aaactaatga aattgatgga
     7801 ctcgatcagc tacctaagat tattttaaca taaacacaca cacacacaca cacacacaca
     7861 cacacacaca cacacacaca cacacagagt tccatttatt tctgtctgtt gctttccttg
     7921 ctctgctctg caagaggagg atgcttttat tgccccaatg tcctaacctt ttttgttgtt
     7981 gttgttattg ttccttttgt ttgggaacaa attgcattgt taattgggta atgaatttta
     8041 agtcctagaa tgtcaaccgt ggtttattta gggaaactat tattaaaaag ataaagaaca
     8101 aaatgttccc caacccagca ttatcatgtg gcttgtcctg gaggagggta ggggtaattt
     8161 tctcgttaat cagccttgta aaagcactta gaagtcaact agggatttag tgagtttgac
     8221 ttctgtgatc ttccctgtct tgtgaggtta ttcttagaat gaatctttag ctatttacct
     8281 gaatttgccc aataaaaaaa caacaaaaca tcagattctc tctttttgct gatattggtt
     8341 ccacccatca ccagggcaag cagaaccaga actgatgtat tgcaaagtga tggtgaattg
     8401 ttttccaatg gcatctgtat gtacatggtc tggggatatg cacatgtgca ctatttttat
     8461 gttcttgcta agccatgtag gccagtggtt atttatccat gtgagcacac agagaaagtc
     8521 tctgcttaaa aaattaatag atttttttta ttctgaacaa taaattgtca ttgacaacat
     8581 tgactaattg ataatggccc catgatatgg tatcaaaaat gttacacgtc tatacgaagg
     8641 tagatcttct ccctgtcatt gtgtgtagag tcaaaaatat cacattacat agaaaacaaa
     8701 gaaacaaaca gataggaaag aaaagaatgt agtgtcagga ttgaaccaag gaaccaaggg
     8761 tgtgctaggt aagagctctg gccctacaac tgaaatctga aagaattttg aagcaaacta
     8821 agctagaatt catgttggtc attaacagat tttctccaga agtcagtggt ttaccctttt
     8881 atgtagacaa actattttag aaattcatta gacttgcata ggctgtatgc atttaaggta
     8941 gctcttatca accggggatg gctttgtggc ccagcagtct ttggcaacat ctgcagacac
     9001 ctttgactgt cataactgag gcgtgctgct ggaatccaat gagtagagat gagagatgct
     9061 gccaaaactc ttccagagga caggaaagac cccccacagc aaagaattgt ctattccaaa
     9121 atgttaatac tgcctaagtt gaaaagctga actaaggcat catttttttc ctctttattt
     9181 tgtttcctgt tcacaacata atatttctat tgattatttg ggaaatctat tacaatggtc
     9241 tctgttaaca gttgcttccc atttctccta agtccaacct ctcacccttt aactctaccc
     9301 acaccaaagc gaaaattaga aagaaggaaa gaaagagaaa agtacaagtt caatttgtgt
     9361 tgcccatata ctcactcact ggagcatgcg aaagtctcag tagccagccc tttaatgata
     9421 actgagttct tcccctacca ctgctagaag ccatagcttc ctttctgcac catgtctaga
     9481 aacaaaacaa agcaaaacaa aaactttctg gtcaaggcac aatctatagc agaacaacaa
     9541 tcaccaactc aagggctttg tatgatgtcc gatgcaaaca tcactttata tagatatgag
     9601 agctgaggtt tagaagaagg agtctgccat ggttttcgtg ggttgttctt tgatagggaa
     9661 gtagcagtcg gtggtgcctg acatcttggt taacccttct gcctcccata ctcgagtata
     9721 ctttgttgat gttgactgtc atgctaatag ctaagagtgc tcattctgaa acatgttgaa
     9781 gcctctgtat tgcaccaccc tgttggaatg ctttctgagc tgatcaacag aagacaaggt
     9841 ttcatgacct aaggaagggg gacaaggtct cagagtgggc taaagatttc atcattttaa
     9901 catctcatgc accctatttc tttgagttga atggccactc attgaagatg gcacttacac
     9961 attgaaaggt atgtttcatt gtttcagaga cttgttcagg gctacctttc atactcactc
    10021 tagaagcctc tgcataagcc ttgggtattg agcctcatct agtatgtacc tgttgactct
    10081 ctgaggaaac ttttataata atttaaaatt ttaaataatt tggccttaaa taatttggta
    10141 tatgttttaa attaatcaga ccaacattaa agtgatagga taaagacagg accatcatag
    10201 ccccattttg caaagtgaaa ctaaagaatg gagagtttaa aatcaatcaa tcagtcaatc
    10261 aatcaatcaa tcaatcattt aatggtttgc atatacattc atcaaatatt ggcaggatgg
    10321 atgtcatgaa aatatcactg taacaaacaa cattaactgc taggaaatag aatactgggt
    10381 aaagaacttg aattcttttg acccaaacat atttttctgt atgttttcta tatttatcat
    10441 acatattatt tgatcaacat attagaatta tcaaatatca tgttttaaat aatgtatatt
    10501 aaggatcttt tatgtcagaa actctactca gtattttaca tatcttatct cgttaaatca
    10561 tttaataatc taatgatgca tccctgccac tctactcacc ttgcagataa gctccaggaa
    10621 gctcagaaag gaaaggcagc acgcatgacc ataaacacag tggcagaaac aagtttcccc
    10681 ctgggcttat tgtatcccaa aggttgtgga cttaaccctc tcagtatcta attgtttctc
    10741 ttttgcattc tgaactttca cctgttctct ttttcattta ctgtaaggga agggtagcta
    10801 gaatgatctt agcccagcac cgtttctaaa aggaacgagt tattttaaac tacaactaca
    10861 accagacatg aatatccaga gaagataaag aaaaggcaaa tgtggaaggt agttctcagt
    10921 tctctttgtc acaggctttt ctttgcactt tgaattctat gggggacact catagtatac
    10981 aactaaggaa accctgtcta ggcctcaggg atactcaccc caggctggct tcagcattaa
    11041 gagagagctg ctcctaagac tgcttgccct tctgtgtact ggcaccacag caagtttcca
    11101 aaggctatgc acatgtgttc atacagatgt gtctatgtat ccttttgctt ttttaagttt
    11161 gacttttagg caaatatttg ttttttttta aattcaagtc agataaaaag tacaaataca
    11221 ttggcttccc gtaatatctc atgattactc ttctatgatg aggagagaca gagataaagc
    11281 aaagaaatga taatgaaata caagagtgtg tgtgtatggg tatgtgtatg tgtgtgtgtg
    11341 tgtacgtgtg taaggagcat gtgtgtgtat gtgttttgtg ttggtgtata aattacatgt
    11401 atatatgtat atatgtatgt acatctatct agatgtatat atagattgct atatatctat
    11461 acatatttat acacataaac acataacttg aaactttaaa attttcagtt taggaaatta
    11521 attatcacat tttatattag tttcctagat agtttattgt agttctggtt tccagaattc
    11581 tcttctacta tgaatgtata tatttattaa tgatacagag aatataatac aaatacctat
    11641 aaaaaacaaa gaaataaaca aaaatgtatt tagaaaaatg ccataccatt ggttggaatt
    11701 tcaacctatg cgtttcaaaa ttgtccactt cagaaacaaa acaaaagaaa aagaaacaag
    11761 aaaccaccaa ataaaagtag ggctttttaa gaataaaaaa aagaaaatga actgatggca
    11821 caatcttata gtcctatgct attacatcac ctgttgatta aattgccgtc tcctagttca
    11881 tggggtctgt gcagcctgca tgattcagag tggagctagt tatctgttta aattattttg
    11941 aagttataac aatcagtttc aaatatgctt tttataaata gctgccaaaa atatctaggg
    12001 cacagtgaag tgataaccaa acaggctcta gccttctgcc aataactctc tcagcctgtt
    12061 tacacaagca ccaaattgtt gctcagtgag gaaagagaga gcagagtcaa agaaaagaaa
    12121 gcaggacttg ttttgatatt ctatagacag aagagccaac tgtctaaatt tgcggcaaaa
    12181 tgtaggctat cttcacagtc agagtcaagt gctttggtga ttgcaggaga atcttgttag
    12241 aggaggttga agccaatcta cgaatgcttc cccggtgctt cttgggttca gtatgtaagc
    12301 acattaactc tgacccttca aagccagtct actccccaaa ccaaggctca gttgatagag
    12361 catgacttct tctcttggga acctatccaa agtgctcact gagccagata cccaggcaca
    12421 ctagtgagca actgaattcc agaagtcctc tgcaggtgtg gtgttgaagg cttgaggaaa
    12481 gaaaatgtct tttctttatg ctttaaaatc aaacgttcta acaaggttca agaattccca
    12541 acattttgga gtggaagggg aactttatat catgggctct ttcacttttc ttttttcttt
    12601 ctttcttttt tttttcaatt tgtccttgca attttttcca cttattaact cttatttttt
    12661 ttaagggaac tgaatcagga gaattttcct aacatcttct atttaagtta gagaataacg
    12721 tggcaatggc gttttggaat gcatgcgggg tggggaagta caggaatcag ccctgctcat
    12781 aaggaactag cctatgcaat tattgaaatg gcatcctatt taaaacactc agagacaatg
    12841 acttacaagg ccttcctttc agaagtctcc ctcaaattgg ctaatgggat aagcttccag
    12901 tgggacacac tttaggctgg tactagtgtt cagctaagtc cataaaagaa cgactctttg
    12961 tgatcgtgag agaaagttta aaagaaatta agttctcatt ccccacattt tacataagca
    13021 gcagagcact aggtagagcc aataaacaac aacaacatca acaaacacct ttcaaattaa
    13081 atgtactgtt tacacaatat tcacgttccc ccttgaccct tcttaactct ggggctgtgt
    13141 tgagtaggca acctggattt ctacagatac attcagcacg gattatcacg tttctttttg
    13201 catctatctc tgatgctttg aaagtgatgt caccacgcat ggaggcggct aaacattgct
    13261 ggaactaaca ccgagtggtc actgacaggg gccttgctca tgttcctttt tgtgctggcc
    13321 tcattgagcc atttgatgat gggccagttt gttgcacatt gatatatact aagtgacact
    13381 cattgaaata acctcttctc tttcccaatg cagtttgctt gtacataagt gtaatgtcaa
    13441 cttgtttaca ggttcttttg tgtataattg tgatagcgga tttagctcaa gccctaatgg
    13501 gatctgttta ctgaatggtg ttaaaacgaa tgtgcttatt acagtataaa catcattaat
    13561 tatggactca catgaatgca tgtctacatc tcatcaattt ccagcagctc agagattcaa
    13621 caggagtctg cagtctgtga atagcaaaca tacataaact gctttgactg ataacttagt
    13681 aactgtttta tcttgctttg taacattgta gatcttagcc tgctgtattt ctttcttata
    13741 tatttaattt ttgagagaaa catagtaaaa aaggaaacta ctgaggtccc aggaaaattg
    13801 cacagcattt tggcaatcgt tggtctattc aaaatgctaa ttactaccat ccatcctcac
    13861 tgccaatgga aacaaactat tttctttgga atcactgaag gatacagaaa gaaataattt
    13921 tttattgact actttcatat tttgtttcat tcttatagta gggtagatga tacaggtgca
    13981 gtaaccgtat tgtaaaactg tgtggcaaaa actacaaaag taattctgtg gatatatttt
    14041 aaaaggcaga acagaaacta tatttcagat tcttgtgtga atataattac aaaaataggt
    14101 aactattttt atgtgtttcc ataatgactc catttcatat acattgtaat ttagcaccaa
    14161 tcataataaa tcatttggta acatttagtt gttatatata tatttttcag tacatataag
    14221 aaattaatga ttcaacatta tttcatgcaa agtcagcatt ttacatgaaa gataattact
    14281 gtttcagtta gtttatttct agtttggtgt ggcagtttgc cacagagtgg tacaaggagg
    14341 aaagtatttg ataagttggt tatttaatat agaaatttaa tatatttaat attaaattgc
    14401 tatcaggtca ataagtggaa gccctacctc tgcctcttgt tgtatggatg gttttacaat
    14461 aatttattat ttatgaaaga aacttcatca ggcaccggtt tctcagtaaa tgtaaagcaa
    14521 tagattggaa gattcttgtg agaattaaat tagataatcc aaggagatat gtcagagtgt
    14581 ttgacacatc aaacagatac aacaataatt cctagagtct cactcaaatc acccctgaaa
    14641 tgataatttt aatgtgggat ttggcttcag aagtctaagt gtctggatat atttattgat
    14701 ctcctgttag taatttatca aatttcctgt aaatatccac tagatttttt taaccccccc
    14761 tccactatga tttttaatct aaagggaaaa ttatagagaa aaggtatttc ctcagataga
    14821 aaatttgaaa ataacttggc cacttgctat ggtgcaatgt ttccaacatt gtttgcactt
    14881 gctatggtgc accctttccc tgcaacacac actcaaaatt agaaagaact ggatggtgca
    14941 gtttgagaaa tagatctttc atgcatccta atttgtaact tcaaactctt cttcataata
    15001 ttgaatgtga actctatgga ttgtgcagta aagaattttt ttttaaactt gttgacccag
    15061 agtctcctac actttgcagt tttgagaccc tcacttggaa attgcggatt catccctggt
    15121 caaatacaag gagagaaaat ctgagactcc aagaagggga ggctctgctt caggtgtgag
    15181 caacctgggt ttggagctcc atctgtggac tcaggctagg gcttctttta cacagctctc
    15241 atgtaaatga ctggtgacag ttagtgtctt attctgactc cacagcacct gttcccatat
    15301 ggactgtgtg catggtcaca agaagccctg aaatccaagc atcagaagga aaggattcca
    15361 aggcataggc atccttagat acagtcatcc atatggtgtg ggctgtaggc tctggtttgt
    15421 ttctatagac tgattttttg gccacctcaa ctaagacaga aattgtccac catactgaac
    15481 ccagcagacc aggggaataa aagcttacac agacaaaagc agaaagaatg tttgagagag
    15541 attgctctta tgcagtagta ggtggaattc tctagggctt ctcctgaaaa atgtacctcg
    15601 tactggtaac atagaaagtg aatgatatcc aaagcatcag agattggtta aagtaactat
    15661 atttcattag actcagccac agggaaagct ctcactccta cctcctgccc tatgatgata
    15721 gatgagaaag aatagcatca agaggaaaag ttggattcaa actttctgac tacagtatca
    15781 aagcatgaac agctgcaagg atttttactg ctcttgatct cagaattgag ttttctcaca
    15841 accccaggct tcaaggatcc tgagctgggg tggtaagaac acttcaccac tctctcattt
    15901 tgcctggaag gtgggggtgg gtcgggtgca tgcatcaggg aaaagacttc tcactagtcc
    15961 ttttcagaga gtgttgggtg gagtgtctcc tcctacatgt cagcgctgca gctataaggc
    16021 agtgtctgat acagcccctt cccagaaact catccctctg ccaaattgat tccaacagga
    16081 catgatcaga aggatcataa ctatggaaca agggcagagg aaatagccca aatcaccacc
    16141 ttcacagaga atatgtctcc tcccctctaa ttttagtcca aaggcagtgt ttctctcaat
    16201 gtctatatga cactcagagc tcccatcaac cccagctcca atggcagctg ccactgaaag
    16261 aggtcatggc aggtgtttat cccaacaggg ggctggcaaa agttcctctc caggcaagac
    16321 agcttcagcc tttaataagg catcctcatc ttgtgagtcc acaaaattat agtatgtcag
    16381 caaaggccaa gcagggtgtg atgttgaatt gcttagaata ccagagctta gactatctct
    16441 ggagccattg tgggtcctta gcaaccatgt caggtgagcc cacaaagcaa agtccagcgt
    16501 tacactcagc cttctccttg attttcttct tcctctggcc cgctgagatg tgtcttatct
    16561 ttagctggta tgaaacgctg ctactctgtg accaggtctg agctcacatg tgctagaggc
    16621 tcttacttgc tacatatcca tgacgaataa tatgaaacaa ttaaatctgc tatctcggag
    16681 acctaaaatc acacggctga atatgcttta tgacataaat aaagcttttt attaaaaaga
    16741 tattttcttt cttaccctcc ctataccttc attcattctt ccttccactc tttattttcc
    16801 tttactttct ttcctaatct aaatggtttt agaaagtatg gagctatgta gcaggggcgg
    16861 ctgggattac aggtatgtac cacaatgcca ggctaaatga tattcttctc ttcattctcc
    16921 tggagtctcc acttcaatct atggatttct gcttctcgaa tttcagtcca tctgatgttc
    16981 cagcatgtgg agagagagag agacagagag agagagagag agagagacag agagagagag
    17041 agagagagag acagagagag agacagagag agagacagag agacagagag acagagagag
    17101 agagagagag agacagagag agagacagag agagagacag agagagagac agagagaggc
    17161 agagagagac agagagaggc agagagagac agagagaggc agagagagac agagatggag
    17221 agaaacagag gtacacacag agaacagtat tttgccaccg ctggacacag gtccttttag
    17281 tttccacata tgctgtgcag tgagcatttg gaggggttat ttctgccgat ggtttttgga
    17341 catttgctat ctttagtctc tacaagtctt cccctaagat gtgtctgatc tcctgtttag
    17401 atgcagagca gaaaggaatc tgtgatgatt cctacagatt caccatttca ggctgtcttt
    17461 atctgggatt ctttggagtg aaaggaaaga cagagagcaa gtgggcccag caatgatgca
    17521 actcacacct ttttggaacg agtcactcca gaactgctgg aaatggagga gctaccctga
    17581 ttcgtcatgt cctaaagttc agttttttct ctgctagtgt atgtgcagcg tggacaactg
    17641 atattccaaa ctactctgag attttcctgg gtcatctggt ccatcttgtg ttatgtgaag
    17701 ccacaccgag tttctgtctt atctttaatg tgaacagaag ctaaagcatt ggcaagaatt
    17761 gggagatagc aagcttagct agacattaga aggaactgag ctggctctga ggttgttgct
    17821 gtatctgttt gtctgtagga agattgaccc gtggtagata caaattataa aaataacttt
    17881 aggaagtttt aaaataaggt aaatgtccta ttctcttgaa acccggcatt aacaaatatg
    17941 aggcagtatg agggggaaag gagacagatt aataataacc agttatgcat tcaggagata
    18001 tggttttgtt ttgctttggg ttttgtctat agagcaaata aatagtgtgg aaattacttg
    18061 ggtctatctt ttaaaagatg tggctacctg tgggatcctt tgcagagtcc aggcttatgc
    18121 tcacagtgga acaaattcca caacatcttt ttgcacctta actttttttt aaataggaat
    18181 gattcttcaa ctaatgtttt tgaaagctga agcaaaagtt tgaatcttga aagtggtcac
    18241 atcctatgtc ttgtcacata gcctatcatg ggtctggcta gctcttatgt accctgaaat
    18301 tatcgtctac agtgtttggg gggaaatagt ggaaaatata gcagatgaag agtcagaaag
    18361 aggaaatata aactgtattt tgatgtactt tttcattaat aaataaatgt taactaaga
//
