LOCUS       NM_008432               3548 bp    mRNA    linear   ROD 19-AUG-2024
DEFINITION  Mus musculus potassium channel, subfamily U, member 1 (Kcnu1),
            mRNA.
ACCESSION   NM_008432
VERSION     NM_008432.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Li,Q., Chen,G. and Yan,J.
  TITLE     Transmembrane determinants of voltage-gating differences between BK
            (Slo1) and Slo3 channels
  JOURNAL   Biophys J 123 (14), 2154-2166 (2024)
   PUBMED   38637987
  REMARK    GeneRIF: Transmembrane determinants of voltage-gating differences
            between BK (Slo1) and Slo3 channels.
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Balbach,M., Hamzeh,H., Jikeli,J.F., Brenker,C., Schiffer,C.,
            Hansen,J.N., Neugebauer,P., Trotschel,C., Jovine,L., Han,L.,
            Florman,H.M., Kaupp,U.B., Strunker,T. and Wachten,D.
  TITLE     Molecular Mechanism Underlying the Action of Zona-pellucida
            Glycoproteins on Mouse Sperm
  JOURNAL   Front Cell Dev Biol 8, 572735 (2020)
   PUBMED   32984353
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3548)
  AUTHORS   Chavez,J.C., Vicens,A., Wrighton,D.C., Andrade-Lopez,K.,
            Beltran,C., Gutierrez,R.M., Lippiat,J.D. and Trevino,C.L.
  TITLE     A cytoplasmic Slo3 isoform is expressed in somatic tissues
  JOURNAL   Mol Biol Rep 46 (5), 5561-5567 (2019)
   PUBMED   31270758
  REMARK    GeneRIF: Study confirms that expression of full-length Slo3 is
            sperm-specific but warns against developing contraceptive drugs
            targeting the C-terminal tail of Slo3 channels.
REFERENCE   4  (bases 1 to 3548)
  AUTHORS   Navarrete,F.A., Alvau,A., Lee,H.C., Levin,L.R., Buck,J., Leon,P.M.,
            Santi,C.M., Krapf,D., Mager,J., Fissore,R.A., Salicioni,A.M.,
            Darszon,A. and Visconti,P.E.
  TITLE     Transient exposure to calcium ionophore enables in vitro
            fertilization in sterile mouse models
  JOURNAL   Sci Rep 6, 33589 (2016)
   PUBMED   27627854
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3548)
  AUTHORS   Stival,C., La Spina,F.A., Baro Graf,C., Arcelay,E., Arranz,S.E.,
            Ferreira,J.J., Le Grand,S., Dzikunu,V.A., Santi,C.M.,
            Visconti,P.E., Buffone,M.G. and Krapf,D.
  TITLE     Src Kinase Is the Connecting Player between Protein Kinase A (PKA)
            Activation and Hyperpolarization through SLO3 Potassium Channel
            Regulation in Mouse Sperm
  JOURNAL   J Biol Chem 290 (30), 18855-18864 (2015)
   PUBMED   26060254
  REMARK    GeneRIF: Considering that capacitation-induced hyperpolarization is
            mediated by SLO3, we evaluated the action of cSrc inhibitors on the
            heterologously expressed SLO3 channel.
REFERENCE   6  (bases 1 to 3548)
  AUTHORS   Martinez-Lopez,P., Santi,C.M., Trevino,C.L., Ocampo-Gutierrez,A.Y.,
            Acevedo,J.J., Alisio,A., Salkoff,L.B. and Darszon,A.
  TITLE     Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by
            Slo3 channels
  JOURNAL   Biochem Biophys Res Commun 381 (2), 204-209 (2009)
   PUBMED   19338774
  REMARK    GeneRIF: native current observed in sperm is indeed carried by Slo3
            channels.
REFERENCE   7  (bases 1 to 3548)
  AUTHORS   Zhang,X., Zeng,X. and Lingle,C.J.
  TITLE     Slo3 K+ channels: voltage and pH dependence of macroscopic currents
  JOURNAL   J Gen Physiol 128 (3), 317-336 (2006)
   PUBMED   16940555
  REMARK    GeneRIF: increases in pH increase the fraction of more rapidly
            opening channels.
REFERENCE   8  (bases 1 to 3548)
  AUTHORS   Zhang,X., Zeng,X., Xia,X.M. and Lingle,C.J.
  TITLE     pH-regulated Slo3 K+ channels: properties of unitary currents
  JOURNAL   J Gen Physiol 128 (3), 301-315 (2006)
   PUBMED   16940554
  REMARK    GeneRIF: voltage and pH regulate a closed-open conformational
            change in a largely independent fashion
REFERENCE   9  (bases 1 to 3548)
  AUTHORS   Shi,J., Krishnamoorthy,G., Yang,Y., Hu,L., Chaturvedi,N.,
            Harilal,D., Qin,J. and Cui,J.
  TITLE     Mechanism of magnesium activation of calcium-activated potassium
            channels
  JOURNAL   Nature 418 (6900), 876-880 (2002)
   PUBMED   12192410
  REMARK    GeneRIF: a structurally new Mg(2+)-binding site in the RCK/Rossman
            fold domain -- an intracellular structural motif that immediately
            follows the activation gate S6 helix -- is responsible for
            Mg(2+)-dependent activation
REFERENCE   10 (bases 1 to 3548)
  AUTHORS   Schreiber,M., Wei,A., Yuan,A., Gaut,J., Saito,M. and Salkoff,L.
  TITLE     Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes
  JOURNAL   J Biol Chem 273 (6), 3509-3516 (1998)
   PUBMED   9452476
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ042506.1, AF039213.2 and BQ175543.1.
            
            On Dec 28, 2006 this sequence version replaced NM_008432.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF039213.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-486               CJ042506.1         2-487
            487-3399            AF039213.2         454-3366
            3400-3548           BQ175543.1         1-149               c
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 14.17 cM"
     gene            1..3548
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /note="potassium channel, subfamily U, member 1"
                     /db_xref="GeneID:16532"
                     /db_xref="MGI:MGI:1202300"
     exon            1..228
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..3399
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /note="potassium large conductance pH-sensitive channel,
                     subfamily M, alpha member 3; slowpoke homolog 3;
                     pH-sensitive maxi potassium channel; calcium-activated
                     potassium channel subunit alpha-3; KSper; pore-forming
                     subunit of the sperm-specific alkalization activated K(+)
                     current; calcium-activated potassium channel, subfamily M
                     subunit alpha-3"
                     /codon_start=1
                     /product="potassium channel subfamily U member 1"
                     /protein_id="NP_032458.3"
                     /db_xref="CCDS:CCDS52530.1"
                     /db_xref="GeneID:16532"
                     /db_xref="MGI:MGI:1202300"
                     /translation="MSQTLLDSLNQKELTETSCTIEIQAAFILSSLATFFGGLIILFL
                     FRIALKSSRSWKYVKGPRGLLELFSSRRIEANPLRKLYFHGVFRQRIEMLLSAQTVVG
                     QVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVHVDLSFNAFFSFYFGLRFWAA
                     EDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKILQILQVIKT
                     SNSVKLSKLLSIVISTWFTAAGFLHLVENSGDPWLNGRNSQTMSYFESIYLVTATMST
                     VGFGDVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFI
                     VVCGNITVDSVTAFLRNFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCG
                     TALKFEDLKRVAVENSEACLILANHFCSDLHDEDNSNIMRVLSIKNYYPQTRVIIQIL
                     QSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVPGLCTFLTTLFIEQNQKVF
                     PKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHKPFFHSC
                     CTLILNPSSQVRLNKDTLGFFIADSSKAVKRAFFYCSNCHSDVCNPELIGKCNCKIKS
                     RQQLIAPTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTV
                     DDTDMLDSSGMFHWCRAMPLDKVVLKRSEKAKHEFQNHIVVCVFGDAQCTLVGLRNFV
                     MPLRASNYTRQELKDIVFIGSLEYFQREWRFLRNFPKIHIMPGSALYMGDLIAVNVEQ
                     CSMCVILATPYKALSSQILVDTEAIMATLNIQSLRITSPTPGSSKSEVKPSSAFDSKE
                     RKQRYKQIPILTELKNPSNIHFIEQMGGLDGMLKGTSLHLSTSFSTGAVFSDTFLDSL
                     LATSFYNYHVVELLQMLVTGGISSEMEHYLVKEKPYKTTDDYEAIKSGRTRCKLGLLS
                     LDQTVLSGINPRKTFGQLFCGSLDNFGILCVGLYRMIDEEEPSQEHKRFVITRPSNEC
                     HLLPSDLVFCAIPFNTTCGKSDSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDL
                     YPVDDTGERWSQHHHSRVYPLDTLDASDIVQEK"
     exon            229..348
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            349..410
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            411..501
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            502..613
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            614..689
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            690..765
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            766..936
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1028
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1139
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1245
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1328
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1398
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1551
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1664
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1733
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1823
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1921
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2057
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2154
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..2379
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2566
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            2567..2644
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..2784
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2979
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            2980..3092
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     exon            3093..3531
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3395..3400
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /note="hexamer: AATAAA"
     polyA_site      3415
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
     regulatory      3506..3511
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /note="hexamer: AATAAA"
     polyA_site      3531
                     /gene="Kcnu1"
                     /gene_synonym="Kcnma3; mSlo3; Slo3"
                     /note="major polyA site"
ORIGIN      
        1 atcatagatt agcccaacag tgtctttctc aagatgtctc aaacattgct agacagttta
       61 aatcagaagg agttgacgga aacgtcatgt acaatcgaaa tccaggcagc gttcattctt
      121 tcctccttgg cgactttctt cgggggactc atcatcttat tccttttcag aatagccttg
      181 aaaagctcaa gaagttggaa atacgtcaag gggccaagag gactcttgga actattctca
      241 tcacgtagaa tcgaggctaa tcctttgagg aaactttact ttcatggagt atttcgtcag
      301 cgcatcgaaa tgctgctttc tgcacagacc gtcgtggggc aagtgttggt gatccttgtc
      361 tttgtactaa gcatcgggtc tcttgtgatc tatttcatca attcaatgga tcctgttcga
      421 aggtgttctt catatgaaga caaaattgtc catgtggatt tgagtttcaa cgctttcttt
      481 agcttctatt ttgggttgag gttttgggca gctgaagaca agatcaagtt ctggttggag
      541 atgaattcaa ttgtagacat ttttaccatc ccgccaacct ttatttctta ttatttgaag
      601 agtaattggc taggtttgag atttctaaga gctctgcggt tgctcgaact ccctaaaatc
      661 ttacagatcc tacaagtcat caagaccagc aattcagtga agctttccaa actgttgtca
      721 atagttatca gtacctggtt cacggcagca ggattccttc acctggtgga aaattctggt
      781 gacccctggc tcaacggaag aaactcacag actatgtcat actttgagtc tatttatctg
      841 gtgacagcaa caatgtcaac tgttggcttt ggggacgtgg tggccaagac atccctagga
      901 cggattttca ttgttttctt cacccttggg agtttgatac tatttgcaaa ctacattcca
      961 gaaatggtgg agctcttttc taccaggaag aaatacacca agccctacga agcagtcaaa
     1021 ggaaaaaagt tcatcgtggt ctgtggaaac atcacagttg acagtgttac tgctttcctg
     1081 aggaattttc tccactggaa gtccggggaa atcaatattg agatcgtatt ccttggagag
     1141 actctccctt gcttggaact ggagacctta ctgaagtgcc acacatcctg taccaacttc
     1201 gtatgcggca ccgcactgaa gttcgaggat ctgaagcgag ttgcagtgga gaactcggag
     1261 gcgtgcctga ttctagccaa ccatttctgt agtgacttac atgacgaaga caactcaaac
     1321 attatgaggg tgctctcgat caagaactat tatccacaga ccagagtcat cattcagata
     1381 cttcagtctc aaaacaaggt tttcctgtca aaaatcccca actgggactg gagtgctgga
     1441 gacaatatcc tctgctttgc agagctaaag ctcggattta tcgcccaagg ctgcttggtg
     1501 ccagggctgt gcacctttct cacgactctg ttcattgaac aaaaccaaaa ggtttttcct
     1561 aaacatccct ggcaaaaaca tttcttgaat ggcttgaaga acaagattct gacacagcgc
     1621 ctctctaacg acttcgtggg gatgacattt ccccaggtct cccggctctg ctttgtgaag
     1681 ctaaatctca tgctgatcgc catccaacac aagcccttct ttcacagttg ttgcactctg
     1741 atactaaacc catcatccca agtgaggctg aataaggaca ccttagggtt cttcattgcg
     1801 gactcctcca aagccgtcaa aagggctttc ttttactgtt ccaactgtca cagcgatgtg
     1861 tgcaatcctg agctaattgg aaagtgtaac tgtaaaatca agagccgaca acaactcata
     1921 gcaccgacca tcatggtgat gaaaagcagc ttgaccgatt tcaccacttc ttcacacatc
     1981 cacgcttcta tgtcaacaga aattcacact tgtttttcaa gagaacagcc tagtttgatc
     2041 accattacaa ccaacagacc aacgacaaac gacacagtgg atgataccga catgctggac
     2101 agcagtggca tgtttcactg gtgcagagca atgcccttgg acaaggtggt tctgaaacga
     2161 agtgagaagg caaaacacga gtttcagaac cacattgtag tatgcgtgtt tggagatgcc
     2221 caatgtaccc tggtggggct tcggaatttc gtgatgcccc tgagagccag caactacacc
     2281 cggcaggagc tgaaggacat tgtttttatt gggtctctgg agtacttcca gagagaatgg
     2341 cgatttctcc gaaactttcc caagatacac attatgcctg gatctgcact ctacatggga
     2401 gatctgattg cagtcaatgt agagcagtgc tctatgtgcg tcatcttagc cacaccctac
     2461 aaggcactga gcagccagat tctggtggac acagaggcca tcatggccac cctcaacatc
     2521 cagtccctgc ggatcaccag tcctactcca gggtcttcaa agtcagaagt aaagccatca
     2581 tctgcctttg atagtaaaga aaggaagcaa agatacaaac agatccccat tctcactgaa
     2641 ctgaagaatc cctccaacat ccactttatt gagcagatgg gcggactgga tggaatgctc
     2701 aaagggacta gcttgcatct cagcacttct ttctccaccg gtgctgtctt ttcagacacc
     2761 ttcttggatt ctctcctggc cacgtccttc tacaattacc atgtcgtgga attacttcag
     2821 atgctagtga ctggaggcat aagctctgag atggaacact atttggttaa ggagaagccc
     2881 tataagacaa ctgacgacta tgaggcaatc aagtctggga ggacgcggtg taagctggga
     2941 ctcctctctt tagaccaaac cgttctatca ggcattaatc caagaaaaac ctttggacag
     3001 ctgttctgtg gctcattgga taatttcggg atcctatgtg tcggcttata ccgtatgatt
     3061 gatgaagagg aacccagcca agaacacaaa aggtttgtga tcaccaggcc atccaatgag
     3121 tgccacctgc tgccctcaga tctcgtgttt tgtgccatcc ctttcaacac cacctgtggc
     3181 aaatcagaca gcagtccttc aattcaggct caaaacaact ctacaaacgc gacgacgcca
     3241 ttggcccagg ggtcgaattt cttcgattcg caccatgccg acgagtccca cgatctttac
     3301 ccagtcgacg acacgggaga gaggtggtct cagcaccacc actcccgagt ctatcctttg
     3361 gacacgttag atgccagtga tattgttcaa gaaaaataaa aacagaaaca tgctattttt
     3421 gtgccagttg cttagtggtc agcgagttgg caagcccacg gtgctgtgga agggaccatc
     3481 ttatcccagt gaatctggtg taatgaataa agatgcccat gataaatggt aaaaaaaaaa
     3541 aaaaaaaa
//
