LOCUS       NM_001081027            8280 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus potassium channel, subfamily T, member 2 (Kcnt2),
            mRNA.
ACCESSION   NM_001081027 XM_001003068
VERSION     NM_001081027.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Hata,K., Wakamori,K., Hirakawa-Yamamura,A., Ichiyama-Kobayashi,S.,
            Yamaguchi,M., Okuzaki,D., Takahata,Y., Murakami,T., Uzawa,N.,
            Yamashiro,T. and Nishimura,R.
  TITLE     Serinc5 Regulates Sequential Chondrocyte Differentiation by
            Inhibiting Sox9 Function in Pre-Hypertrophic Chondrocytes
  JOURNAL   J Cell Physiol 240 (1), e31490 (2025)
   PUBMED   39568258
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 8280)
  AUTHORS   Werren,E.A., LaForce,G.R., Srivastava,A., Perillo,D.R., Li,S.,
            Johnson,K., Baris,S., Berger,B., Regan,S.L., Pfennig,C.D., de
            Munnik,S., Pfundt,R., Hebbar,M., Jimenez-Heredia,R.,
            Karakoc-Aydiner,E., Ozen,A., Dmytrus,J., Krolo,A., Corning,K.,
            Prijoles,E.J., Louie,R.J., Lebel,R.R., Le,T.L., Amiel,J.,
            Gordon,C.T., Boztug,K., Girisha,K.M., Shukla,A., Bielas,S.L. and
            Schaffer,A.E.
  TITLE     TREX tetramer disruption alters RNA processing necessary for
            corticogenesis in THOC6 Intellectual Disability Syndrome
  JOURNAL   Nat Commun 15 (1), 1640 (2024)
   PUBMED   38388531
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8280)
  AUTHORS   Liu,B., Ye,B., Zhu,X., Yang,L., Li,H., Liu,N., Zhu,P., Lu,T.,
            He,L., Tian,Y. and Fan,Z.
  TITLE     An inducible circular RNA circKcnt2 inhibits ILC3 activation to
            facilitate colitis resolution
  JOURNAL   Nat Commun 11 (1), 4076 (2020)
   PUBMED   32796851
  REMARK    GeneRIF: An inducible circular RNA circKcnt2 inhibits ILC3
            activation to facilitate colitis resolution.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8280)
  AUTHORS   Reijntjes,D.O.J., Lee,J.H., Park,S., Schubert,N.M.A., van
            Tuinen,M., Vijayakumar,S., Jones,T.A., Jones,S.M., Gratton,M.A.,
            Xia,X.M., Yamoah,E.N. and Pyott,S.J.
  TITLE     Sodium-activated potassium channels shape peripheral auditory
            function and activity of the primary auditory neurons in mice
  JOURNAL   Sci Rep 9 (1), 2573 (2019)
   PUBMED   30796290
  REMARK    GeneRIF: Sodium-activated potassium channels shape peripheral
            auditory function and activity of the primary auditory neurons in
            mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8280)
  AUTHORS   Rizzi,S., Knaus,H.G. and Schwarzer,C.
  TITLE     Differential distribution of the sodium-activated potassium
            channels slick and slack in mouse brain
  JOURNAL   J Comp Neurol 524 (10), 2093-2116 (2016)
   PUBMED   26587966
  REMARK    GeneRIF: This study showed that the Intense Slick channel
            immunoreactivity was observed in processes, varicosities, and
            neuronal cell bodies of the olfactory bulb, granular zones of
            cortical regions, hippocampus, amygdala, lateral septal nuclei,
            certain hypothalamic and midbrain nuclei, and several regions of
            the brainstem.
REFERENCE   7  (bases 1 to 8280)
  AUTHORS   Wojtovich,A.P., Smith,C.O., Urciuoli,W.R., Wang,Y.T., Xia,X.M.,
            Brookes,P.S. and Nehrke,K.
  TITLE     Cardiac Slo2.1 Is Required for Volatile Anesthetic Stimulation of
            K+ Transport and Anesthetic Preconditioning
  JOURNAL   Anesthesiology 124 (5), 1065-1076 (2016)
   PUBMED   26845140
  REMARK    GeneRIF: Slick (Slo2.1) is required for both volatile
            anesthetics-stimulated K flux and for the anesthetic
            preconditioning-induced cardioprotection
REFERENCE   8  (bases 1 to 8280)
  AUTHORS   Martinez-Espinosa,P.L., Wu,J., Yang,C., Gonzalez-Perez,V., Zhou,H.,
            Liang,H., Xia,X.M. and Lingle,C.J.
  TITLE     Knockout of Slo2.2 enhances itch, abolishes KNa current, and
            increases action potential firing frequency in DRG neurons
  JOURNAL   Elife 4, e10013 (2015)
   PUBMED   26559620
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8280)
  AUTHORS   Oeschger,F.M., Wang,W.Z., Lee,S., Garcia-Moreno,F., Goffinet,A.M.,
            Arbones,M.L., Rakic,S. and Molnar,Z.
  TITLE     Gene expression analysis of the embryonic subplate
  JOURNAL   Cereb Cortex 22 (6), 1343-1359 (2012)
   PUBMED   21862448
REFERENCE   10 (bases 1 to 8280)
  AUTHORS   Yang,B., Desai,R. and Kaczmarek,L.K.
  TITLE     Slack and Slick K(Na) channels regulate the accuracy of timing of
            auditory neurons
  JOURNAL   J Neurosci 27 (10), 2617-2627 (2007)
   PUBMED   17344399
  REMARK    GeneRIF: Slick and Slack are expressed at high levels auditory
            brainstem. Activation of these KNa channels allows temporal
            accuracy of firing to be increased at high frequencies of
            stimulation.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC120400.11, AC156607.5 and AC156989.3.
            
            On Feb 15, 2019 this sequence version replaced NM_001081027.2.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data because no single transcript was available for the full length
            of the gene. The extent of this transcript is supported by
            orthologous data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660821.195829.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-634               AC120400.11        45139-45772         c
            635-714             AC156607.5         87004-87083         c
            715-814             AC156607.5         83738-83837         c
            815-863             AC156607.5         75294-75342         c
            864-923             AC156607.5         72707-72766         c
            924-998             AC156607.5         63109-63183         c
            999-1082            AC156607.5         61855-61938         c
            1083-1177           AC156607.5         55196-55290         c
            1178-1358           AC156607.5         12918-13098         c
            1359-1523           AC156607.5         11299-11463         c
            1524-1660           AC156607.5         9346-9482           c
            1661-1724           AC156607.5         6858-6921           c
            1725-1833           AC156989.3         20807-20915
            1834-1942           AC156989.3         54736-54844
            1943-2092           AC156989.3         78311-78460
            2093-2322           AC156989.3         79995-80224
            2323-2536           AC156989.3         83432-83645
            2537-2642           AC156989.3         89477-89582
            2643-2815           AC156989.3         93699-93871
            2816-2887           AC156989.3         101364-101435
            2888-3022           AC156989.3         104472-104606
            3023-3134           AC156989.3         123571-123682
            3135-3236           AC156989.3         140993-141094
            3237-3320           AC156989.3         144206-144289
            3321-3449           AC156989.3         145340-145468
            3450-3750           AC156989.3         154630-154930
            3751-3835           AC156989.3         166586-166670
            3836-8280           AC156989.3         180212-184656
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 61.62 cM"
     gene            1..8280
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /note="potassium channel, subfamily T, member 2"
                     /db_xref="GeneID:240776"
                     /db_xref="MGI:MGI:3036273"
     exon            1..634
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     CDS             540..3947
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /note="sodium- and chloride-activated ATP-sensitive
                     potassium channel subunit; sequence like an intermediate
                     conductance potassium channel subunit; sodium and
                     chloride-activated ATP-sensitive potassium channel Slo2.1"
                     /codon_start=1
                     /product="potassium channel subfamily T member 2"
                     /protein_id="NP_001074496.2"
                     /db_xref="CCDS:CCDS48389.1"
                     /db_xref="GeneID:240776"
                     /db_xref="MGI:MGI:3036273"
                     /translation="MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTF
                     KERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGSEWSHIFWVNRSL
                     PLWGLQVSVALISLFETILLGYLSYKGNIWEQILRIPFILEIINAVPFIISIFWPTLR
                     NLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQ
                     HLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQ
                     LAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVI
                     LCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDR
                     TSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCI
                     CPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSF
                     TYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQ
                     DQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRATSGPTLALPSEGGKEL
                     RRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSP
                     TFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLR
                     AYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANM
                     VVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCY
                     SLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLL
                     GLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESQ
                     ISISVEEWEDTKDVKDPGHHRSLHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSG
                     KTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPS
                     PDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL"
     exon            635..714
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            715..814
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            815..863
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            864..923
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            924..998
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1082
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1177
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1358
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1523
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1660
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1724
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1833
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1942
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2092
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2322
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2536
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2537..2642
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2815
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2816..2887
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2888..3022
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3134
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3135..3236
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3237..3320
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3321..3449
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3450..3750
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3751..3835
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3836..8280
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6277..6282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /note="hexamer: AATAAA"
     polyA_site      6302
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /note="major polyA site"
     regulatory      6568..6573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /note="hexamer: AATAAA"
     polyA_site      6589
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
     regulatory      8249..8254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
                     /note="hexamer: AATAAA"
     polyA_site      8268
                     /gene="Kcnt2"
                     /gene_synonym="E330038N15Rik; Slick"
ORIGIN      
        1 agtgcttgca ctccctgcga gggagctgtg gccggggatg ccctagtggg aggctctggt
       61 ccttgccttt caggaaagtg cctagccatc acgcggtgct cacacccacc acctaccctc
      121 tttctaggca ccgcgcgaat tttcttttcc ttttcttttc tttctttttt aaatttttcc
      181 ttttcctttt tttttttttt ctgtccgtga agtaactttt gcattcccgc ctccccccgg
      241 tcccagggac cagaggcggt gccgagcctg ggggcggttc cttggcagcg ctggttcgag
      301 tcgcagccag acttttgctc ctgggctcgg tttgcatcat ccccatcaca cccttgggga
      361 agaggagcca cccctttcca gcctcagctt ccggccgaac gacccctccc cctccgggtc
      421 ccccaagccc ttctcccacc ctccccagca cggcaccccc tccgcagtgc ttgctacccc
      481 tctttgccct cctccatccc tccatccctt gttggatgtt gcccactcct taaggaagga
      541 tggttgattt ggagagcgaa gtgccccctc tgcctcccag gtacagattc cgggatttgc
      601 tgctagggga ccaaggctgg caaaatgatg acagggtcca agttgaattc tacatgaacg
      661 aaaacacatt taaagagaga ttaaaactgt ttttcataaa aaaccagcga tcaagtctaa
      721 gaatccgctt gttcaacttt tccctgaagt tgttaagctg cttattatat attatccgtg
      781 tgttgcttga gaaaccgtca cagggaagtg aatggtctca catattttgg gttaaccgaa
      841 gtctaccttt atggggttta caggtctcag tggcattgat aagtctattt gaaacaatac
      901 tccttggtta tctcagttat aagggaaata tctgggagca gattttacga attccattca
      961 tcttggaaat aatcaatgca gtccccttca tcatctcaat attctggccc accttaagaa
     1021 atctatttgt tccagttttt ctaaattgtt ggcttgccaa acatgccttg gaaaatatga
     1081 ttaatgatct ccatagagcc attcagcgta cacagtctgc aatgtttaat caagttttga
     1141 ttttgatatc tactttacta tgccttatct tcacttgcat ttgtgggatt cagcatctag
     1201 aacgaattgg caagaagctc aatctctttg actcccttta tttctgcatt gtgacatttt
     1261 ctactgtggg ctttggggat gtcactcctg aaacatggtc ctccaagctc tttgtcgttg
     1321 ctatgatctg cgttgccctt gtagtactcc ctatacagtt tgaacagttg gcctatttgt
     1381 ggatggagag acaaaagtca ggaggaaact acagtcgaca cagagctcag acagagaagc
     1441 atgttgtcct gtgtgtcagc tccttgaaga ttgacttact tatggatttt ctaaatgagt
     1501 tctatgcgca tccaagacta caggattatt acgtggtgat tttgtgtccc acggagatgg
     1561 atgtgcaagt tcggagagta ctacagattc caatgtggtc ccagagagtg atctaccttc
     1621 aaggctctgc ccttaaagac caggatctcc tgagagcaaa gatggacaat gctgaggcct
     1681 gtttcatttt gagtagccgc tgcgaagtgg atagaacctc atctgatcac caaacaatct
     1741 tgagagcatg ggctgtgaaa gattttgccc caaattgtcc tctgtatgtt cagatattaa
     1801 aaccagaaaa caaattccac atcaaatttg cagatcatgt tgtttgtgaa gaagagttta
     1861 agtacgccat gttagcttta aactgtatat gcccagcaac atctacactc attacactac
     1921 tggttcatac ctctagaggg caagaagggc agcagtcacc tgaacagtgg cagaagactt
     1981 acgggaggtg ctcaggaaac gaagtctatc acatcgtttt ggaagaaagt acattttttg
     2041 ctgaatatga agggaagagt tttacctatg cttcttttca tgcccacaaa aagtttggtg
     2101 tctgcttggt tggtgttagg agggaggata ataaaaacat tttgctgaat ccaggtcctc
     2161 gatacattat gaatgcttca gacatatgtt tttatattaa tattaccaaa gaagaaaatt
     2221 cagcatttaa gaatcaagac caacagagaa aaagtaatgt gtcaaggtca ttttatcatg
     2281 ggccttccag attgcctgtc cacagcatca ttgccagcat gggtactgtg gctatagact
     2341 tgcaagacac aagctgcaga gcaacaagtg gccccaccct ggctcttcct tcagagggag
     2401 gcaaagaatt aagaagacct agcattgctc ctgttttaga ggtggcagat acatcatcaa
     2461 ttcaaacatg cgatctccta agtgaccagt ccgaagatga aactacacca gatgaagaaa
     2521 catcttcaaa tttagagtat gccaaaggct acccacctta ttccccatac ataggaagtt
     2581 cacctacttt ttgtcaccta cttcaagaaa aagtgccatt ttgctgctta agattagaca
     2641 agagttgcca gcataactac tatgaggacg caaaagccta tggattcaaa aataaactaa
     2701 ttatagttgc agctgaaaca gctggaaatg gactatataa ttttattgta cctctcaggg
     2761 catattatag accaaagaaa gaattaaatc ccatagttct gctattggat aacccgccag
     2821 atatgcattt tctggatgca atctgttggt ttccaatggt ttactacatg gtgggctcta
     2881 ttgacaacct agatgatttg ctcaggtgtg gagtgacctt tgctgccaac atggtggtgg
     2941 tggacaaaga aagcaccatg agtgcagagg aagactacat ggcagatgcc aagacgattg
     3001 tgaatgtgca gactctgttc aggttgtttt caagtctcag tatcatcact gagcttacgc
     3061 atccagcaaa tatgagattc atgcaattca gagccaagga ttgttactct ctcgcgcttt
     3121 caaaactgga aaagaaagaa agagaacgag gttctaactt ggcctttatg tttcgactgc
     3181 cttttgctgc gggaagagtg ttcagcatca gtatgttaga cacccttctt tatcagtcat
     3241 ttgtgaaaga ttatatgatt tctatcacca gacttctttt gggactggac accataccag
     3301 gatcggggtt tctttgttct atgaaaatca ctgaagatga cttgtggatc agaacgtatg
     3361 ccagactcta tcagaagttg tgttcttcta ctggagatgt tcccatcggg atctacagaa
     3421 cagaatctca gaaactaacg acatctgagt ctcaaatatc cattagtgtg gaagagtggg
     3481 aagacaccaa agatgtcaaa gacccagggc accaccgcag ccttcaccgc aactcaacgt
     3541 ctagtgacca gtcggaccat cccttgctgc ggaggaagag catgcagtgg gcccgaaggc
     3601 tgagcagaaa agggccgaag cactctggta aaactgcaga aaaaataact caacagcgac
     3661 tgaacctcta ccggagatcc gaaagacagg agcttgctga acttgtgaaa aacagaatga
     3721 aacacctggg cctctcgacg gtgggctacg atgaaatgaa tgatcatcag agcaccctgt
     3781 cctacatcct gattaatccg tctccagaca ccaggcttga gctgaatgat gttgtatatt
     3841 tgatccgacc agatccactg tcctaccttc caaacagtga acccagtagg aaaaacagca
     3901 tctgcaatgc cgctgttcaa gattctcggg aggaaactca actctgatga aaagaaaata
     3961 agagacattg tagtccttgc agggatcttg cttaaaatca ttaaaatgtt gcagacagtc
     4021 ctaagagcct gactggaatg tattcagttt ctcatgttac ttctatttaa aaaataaaat
     4081 ctattctctt agaagtacag atcattttga aacttaatgt gatatttgtt gacattttcc
     4141 tggctaatat ttaaaattca ttggaaaaga ttttcaaaat taatataaag tgcagaagtt
     4201 aaaaatctaa tatttagttg tttaggaata atcacaagta tcggcagtca attttaaagc
     4261 attattaaga ctgtatgaac atttaaaaat ccacctatat taagtacatc acaagaacac
     4321 agaggactga ttttcttttt ataatttaat atgtcattat attgtttaaa attgctgtct
     4381 ctaaaattga acccattaag aattaatgct tatcattatg gagagattat ccattaactt
     4441 tcatggctga cttctgtcta ggcatcatag tttgcttcag aatagatctt tatcatcatg
     4501 tctgcctttc catactattg tatttatttt agaaactgag atatacatag catgattact
     4561 tttacagaag aaactaaaag acaaaggaat attaagcacc tgaggggttt ttgtttgatt
     4621 gattgtttgt ttttggtaac tttacatcaa atacacattt tatgctagaa aaagtgtact
     4681 taataggaat tatttttgtt ttcctaaaga aaatgcctaa tatagagaaa acaatacata
     4741 tctttacagc ttatttgaat gtgagaattg gaatattacc tgtatatatc agatgagcag
     4801 tgatgacttc tataactgtt ggctttaata tttttcattt agctatataa taaaattcac
     4861 taggaaaatc atttaaagtt gaaagaaaaa tatttatata ttttatgaac cacaaggaca
     4921 ctttaaaacc ttaggaatta taaaaatgtc tttgatatta gatactcaca atcaaagtct
     4981 gatttaatgt aaaaattaat aaaatgtgtc tatacttagc attgtaagga aaaacatcat
     5041 taccatagat atttattata tttgaaatct aatatgaatt gttcagaatt tgaaacataa
     5101 atttaatata acctgactat tgaaacacta atacttttag ctttattccc taaatttgat
     5161 ttcataaaca tgctaaaatt tgcaagagaa ttaacacata cacagtttgc caaatcatat
     5221 caaatcttcc cattggagat atattagtac acttaaaata cctaatattc tgatttttat
     5281 ctatttcttt attcataaag ttggcacaga gtcacaatgc aacctaaagt agttcatgta
     5341 agaaacagaa tattttgtcc tttcttctca tactaaagtg gatcatagaa aagtaaaata
     5401 aattatttga aaaatgaatc cttatgtcct tcccattatt atagagaaaa atagaaagat
     5461 aaaatgtaaa atcaaatctt caggcaatct tacaatttga ccagagaata ttttttatta
     5521 gtactagttg ttattaccta taattgagaa taagaaaaat atggaaagtt ccctcaaaac
     5581 agagagatgc tctctagtgt ttagaaaagt atggtagaac aagttgctta taattgagag
     5641 tgagagcatg ccttaaatgt catgatggta tgtgaatatg tgtgtatgtt gcaaacagga
     5701 aatgcacttg taaacatacg acatgatgtt aaaattgaaa gcaggactga actaacgtat
     5761 tcaatattga atatgtagat atcagtagtc aagtaggata tttctttaga ttgccacttt
     5821 ttttattcca gttgttttag gaataaatgt cagattttaa aagtcttcta tttcccaatg
     5881 atctctgaag tttagtattt ttgcactatg acaattgcta agactgggat tttaagctag
     5941 gatatgatta tatttcctat aaaactaaaa cttttatttc ataaatttta aaataattat
     6001 ttttgattat gaatactagt ccaaatttaa tatttgacag aattcgtatt gcttgctata
     6061 acaaggatag tttatagtct ttccagttat ttaatgaata aatgacgttt attaaaaaca
     6121 ttcaacaaat tgttgccttt ttaaaattat cacacttgca catgaagata aaacatgaag
     6181 taattgtgct ggtagccttt tgggaattgg tttggtttat tgtatatgta ccattttgct
     6241 accagtttca ttgaattgat ttaaaaataa ataaataata aaattatttc tgatgatgaa
     6301 aattccttgt tttttttttt aataacagaa ctttggacaa attctgtttt gaaactgtaa
     6361 aagtattttg tcatttttat atctcaaaca ccaaataaat gtgaaagtta ttcatttgag
     6421 aggcaaatta aaataaattt aattctgcag cctgtggata aatactgcag gaaagaagaa
     6481 gggaaacgag gaggagagag agaaaagagg tacacaatag gaaggaaact ataaagaggg
     6541 tgtggagagg gaaaatgtaa gcaccaaaat aaaaacatat tttaaaaaag agtgtgttag
     6601 gtcctattat gttcaattat aaattcagaa aaaaatcacc atgcaagtga aaattaaaat
     6661 tgttttaaac attggatggt cttttctttg aggcaaatag tagacaccat tctccctgtt
     6721 tctgctgcta tgggatgtat ttgatgagaa catagatttc gccatgggga tacaagtaga
     6781 aacagaacca tggcaaagtg ggccattgag acccctgaga ttttgttttg tctcccataa
     6841 tcctattcat gatgttgaga aacttaatgg acatagacag tccctggaaa gcatattctg
     6901 tcctctctaa aaactaactc ctgaagcaaa gtgatgttgc aggatccaga ctactcaggc
     6961 agctttggct tttgcaagat ggtgtatgct ttggacaagg ggttttgcaa tgtacagtgg
     7021 ttggcacaca gtggcttaaa gaagctgtca cagtgaggta ggccttaggg aaagtgttgc
     7081 ggatgaagta tgcctcagaa aaacttttcc ctagaacctt gaatcagaat tttagtgagt
     7141 acatctacaa tagcaaagtt gtgactgctt tccttggtat gagatcctac agtgctgcct
     7201 tcaagtgaac ctagaactag tttgagggct ttcccccaga aacctttatc ttgaccctga
     7261 agctgtggat actgcttata tagtacttca aggttttcat attctctttg ctaaccagtc
     7321 tgtttttact ccaaggtccc cttaggagtt aattcttccc caccagcatt tttccatgct
     7381 cctgagaggt ttcttgacca tcacaatgaa gattgactag gggataaaac tcctttatat
     7441 caaaggagat taatttatga tactatctaa gaggaaaatg aggaaaaaca aaacaaaacc
     7501 ctcaagggac acataaaata gtaaaatcaa agtacagaaa aatgggacat gaacatggaa
     7561 tggaacagag tcacagttca cagttctgtt tagagttgta catatgccaa gaaaagcaac
     7621 tcatagaatt tactataaag gatttcagaa ccaagatcat tactccagag aggagtaata
     7681 agttcacata agaacgtctt ctttaaaaat gccattggag ttcacaaaag gatacagtac
     7741 actgcttgga cccttggcat tcaaatgctt gggaggagca ttttgtgcgg agtttgatat
     7801 tcctgcttag gactctactg ctgctcctat tgatgctttg gactactaca taagaacaga
     7861 tattccttga acagtgacag acacaacaga aaattaccag ataatatatg gactaaagag
     7921 aaatatttta ggcaattgca tttattttta agtctttgct atgttgaaaa actgtcatct
     7981 gggacctgat tttattattt tttttgttta tccttattat acagaatctg aaacagtttt
     8041 gctgcatgct aattgctttg ttaacaaaat agaattttgc gacttttttt ctgtgcactt
     8101 aaaagcaata cagtagtcta catatttatt ggacttaact ttccttatta ctattgcagt
     8161 ttaaatctat ataaaataaa atattataat aaagtcatgg atgtggaaaa tgctttgtat
     8221 ataagttgat gttattttta caatgaagaa taaatcttaa attgaaaact gttgatacta
//
