LOCUS       NM_175462               5897 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus potassium channel, subfamily T, member 1 (Kcnt1),
            transcript variant 1, mRNA.
ACCESSION   NM_175462 XM_622105
VERSION     NM_175462.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Shore,A.N., Li,K., Safari,M., Qunies,A.M., Spitznagel,B.D.,
            Weaver,C.D., Emmitte,K., Frankel,W. and Weston,M.C.
  TITLE     Heterozygous expression of a Kcnt1 gain-of-function variant has
            differential effects on somatostatin- and parvalbumin-expressing
            cortical GABAergic neurons
  JOURNAL   Elife 13, 92915 (2024)
   PUBMED   39392867
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Wu,J., Quraishi,I.H., Zhang,Y., Bromwich,M. and Kaczmarek,L.K.
  TITLE     Disease-causing Slack potassium channel mutations produce opposite
            effects on excitability of excitatory and inhibitory neurons
  JOURNAL   Cell Rep 43 (3), 113904 (2024)
   PUBMED   38457342
  REMARK    GeneRIF: Disease-causing Slack potassium channel mutations produce
            opposite effects on excitability of excitatory and inhibitory
            neurons.
REFERENCE   3  (bases 1 to 5897)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 5897)
  AUTHORS   Tobin,W.F. and Weston,M.C.
  TITLE     Distinct Features of Interictal Activity Predict Seizure
            Localization and Burden in a Mouse Model of Childhood Epilepsy
  JOURNAL   J Neurosci 43 (27), 5076-5091 (2023)
   PUBMED   37290938
REFERENCE   5  (bases 1 to 5897)
  AUTHORS   Burbano,L.E., Li,M., Jancovski,N., Jafar-Nejad,P., Richards,K.,
            Sedo,A., Soriano,A., Rollo,B., Jia,L., Gazina,E.V., Piltz,S.,
            Adikusuma,F., Thomas,P.Q., Kopsidas,H., Rigo,F., Reid,C.A.,
            Maljevic,S. and Petrou,S.
  TITLE     Antisense oligonucleotide therapy for KCNT1 encephalopathy
  JOURNAL   JCI Insight 7 (23), e146090 (2022)
   PUBMED   36173683
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5897)
  AUTHORS   Kaczmarek,L.K.
  TITLE     Slack, Slick and Sodium-Activated Potassium Channels
  JOURNAL   ISRN Neurosci 2013 (2013) (2013)
   PUBMED   24319675
REFERENCE   7  (bases 1 to 5897)
  AUTHORS   Yang,B., Desai,R. and Kaczmarek,L.K.
  TITLE     Slack and Slick K(Na) channels regulate the accuracy of timing of
            auditory neurons
  JOURNAL   J Neurosci 27 (10), 2617-2627 (2007)
   PUBMED   17344399
  REMARK    GeneRIF: Slick and Slack are expressed at high levels auditory
            brainstem. Activation of these KNa channels allows temporal
            accuracy of firing to be increased at high frequencies of
            stimulation.
REFERENCE   8  (bases 1 to 5897)
  AUTHORS   Paulais,M., Lachheb,S. and Teulon,J.
  TITLE     A Na+- and Cl- -activated K+ channel in the thick ascending limb of
            mouse kidney
  JOURNAL   J Gen Physiol 127 (2), 205-215 (2006)
   PUBMED   16446508
  REMARK    GeneRIF: This is the first demonstration of an epithelial cell
            membrane, Na+-activated, large-conductance K+ channel resembling
            K(Na) channels of excitable cells. The Slo2.2 type, Na+- and
            Cl--activated K+ channel is located in the ascending limb of the
            kidney.
REFERENCE   9  (bases 1 to 5897)
  AUTHORS   Uchino,S., Wada,H., Honda,S., Hirasawa,T., Yanai,S., Nakamura,Y.,
            Ondo,Y. and Kohsaka,S.
  TITLE     Slo2 sodium-activated K+ channels bind to the PDZ domain of PSD-95
  JOURNAL   Biochem Biophys Res Commun 310 (4), 1140-1147 (2003)
   PUBMED   14559234
  REMARK    GeneRIF: partly colocalized with PSD-95 in mouse neocortical
            neurons
REFERENCE   10 (bases 1 to 5897)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731682.20.
            
            On Oct 27, 2022 this sequence version replaced NM_175462.4.
            
            Summary: This gene encodes a member of the Slo potassium channel
            family that has shown to be activated by both sodium and chloride
            ions. This channel represents the largest potassium channel subunit
            yet identified. This channel may be important in development and
            pain signaling. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Oct 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK033953.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-179               AL731682.20        78355-78533
            180-221             AL731682.20        82694-82735
            222-301             AL731682.20        88567-88646
            302-401             AL731682.20        89257-89356
            402-458             AL731682.20        91347-91403
            459-507             AL731682.20        91689-91737
            508-567             AL731682.20        92556-92615
            568-642             AL731682.20        92832-92906
            643-726             AL731682.20        92972-93055
            727-821             AL731682.20        93627-93721
            822-1002            AL731682.20        94780-94960
            1003-1167           AL731682.20        98769-98933
            1168-1304           AL731682.20        99197-99333
            1305-1368           AL731682.20        100721-100784
            1369-1477           AL731682.20        100870-100978
            1478-1586           AL731682.20        101387-101495
            1587-1736           AL731682.20        101707-101856
            1737-1975           AL731682.20        102250-102488
            1976-2210           AL731682.20        103857-104091
            2211-2316           AL731682.20        105771-105876
            2317-2489           AL731682.20        107161-107333
            2490-2561           AL731682.20        107773-107844
            2562-2696           AL731682.20        108014-108148
            2697-2808           AL731682.20        108543-108654
            2809-2910           AL731682.20        109703-109804
            2911-2994           AL731682.20        110092-110175
            2995-3117           AL731682.20        110312-110434
            3118-3138           AL731682.20        110764-110784
            3139-3469           AL731682.20        111524-111854
            3470-3532           AL731682.20        113153-113215
            3533-3620           AL731682.20        116277-116364
            3621-5897           AL731682.20        116495-118771
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 18.27 cM"
     gene            1..5897
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /note="potassium channel, subfamily T, member 1"
                     /db_xref="GeneID:227632"
                     /db_xref="MGI:MGI:1924627"
     exon            1..179
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..3741
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium-activated potassium channel; potassium channel
                     subunit homolog"
                     /codon_start=1
                     /product="potassium channel subfamily T member 1 isoform
                     1"
                     /protein_id="NP_780671.2"
                     /db_xref="CCDS:CCDS15794.1"
                     /db_xref="GeneID:227632"
                     /db_xref="MGI:MGI:1924627"
                     /translation="MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGS
                     VGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFS
                     LKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVI
                     QVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIP
                     VFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERA
                     GGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLW
                     MERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSE
                     MDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADH
                     QTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATS
                     TLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAF
                     HAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQ
                     KRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTEN
                     GSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPY
                     IGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNF
                     IVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGII
                     YADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFR
                     AKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITI
                     TRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFS
                     EPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRK
                     SLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTT
                     GYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRS
                     DPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL"
     exon            180..221
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            222..301
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            302..401
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            402..458
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            459..507
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            508..567
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            568..642
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            643..726
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            727..821
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            822..1002
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1167
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1304
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1368
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1477
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1586
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1736
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1975
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2210
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2316
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2489
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2561
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2562..2696
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2808
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..2910
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2911..2994
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3117
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3118..3138
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3139..3469
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3470..3532
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3533..3620
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3621..5897
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4217
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /note="major polyA site"
     regulatory      4684..4689
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /note="hexamer: ATTAAA"
     polyA_site      4718
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /note="major polyA site"
     regulatory      5872..5877
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
                     /note="hexamer: AATAAA"
     polyA_site      5897
                     /gene="Kcnt1"
                     /gene_synonym="C030030G16Rik; Slack; slo2; Slo2.2"
ORIGIN      
        1 acccgcgcca agcgctcccg caggatggcg cgggccaagc tgccgcgctc gccgtccgag
       61 ggcaaggcgg gtccggggga caccccagcc ggcgctgcag cccctgagga gccacacggg
      121 ctcagcccgc tactgccggc ccgcggcggg ggctccgtgg gcagcgacgt gggccagagg
      181 cttcatgtgg aagatttcag cctggactct tccctttctc aggtccaggt ggagttctat
      241 gtcaatgaga acaccttcaa agagcggctc aagctgttct tcatcaaaaa ccaaagatcc
      301 agcctgagga tccggctgtt caacttctcc ctcaagctcc tcacctgcct gctgtatatc
      361 gtccgtgtcc tgcttgacaa cccagaccag ggcatcggat gctggggctg cacgaagtat
      421 aactacactt tcaatggctc atcctctgag ttccactggg ctcccatcct gtgggtggag
      481 aggaaaatgg ctctgtgggt gatccaggtc attgtggcca caataagctt cttagagacc
      541 atgctcatca tttacctcag ctacaaaggc aacatctggg agcagatatt ccatgtgtct
      601 ttcgtcttgg agatgatcaa cacactgccc ttcatcatca cggtcttctg gccacctctg
      661 cggaacctgt tcatccccgt gtttctcaac tgctggctgg ccaagcatgc gctggagaac
      721 atgattaatg acttccaccg tgccatccta cgcacacagt cagccatgtt caaccaggtg
      781 ctcatcctgt tctgcaccct gctgtgcctg gtcttcacag ggacctgtgg gatccagcac
      841 ctggagcgag caggtggcaa cttgaacctg ctgacctcct tctacttctg catcgtgact
      901 ttctcaactg tgggcttcgg tgatgtgacg cccaagatct ggccatccca gctcctggtg
      961 gtcatcctga tctgtgtcac ccttgtggtg ctcccactgc aatttgaaga gcttgtctac
     1021 ctctggatgg agcgtcagaa gtcagggggc aactatagcc gccaccgagc acggacggag
     1081 aagcacgtag tcctgtgtgt gagctccctc aagattgatc tcctcatgga cttcctgaat
     1141 gagttctatg cccatccccg gctccaggac tactacgtgg tcatcctgtg tccctctgaa
     1201 atggacgtcc aggtgcgcag ggtgctgcag attcccctgt ggtcccagcg ggtcatctac
     1261 ctccagggct ctgccctcaa ggaccaggat ctcatgcgag ccaagatgga caacggagag
     1321 gcctgcttta tcctcagcag caggaatgag gtggaccgca cagctgcgga tcaccagacc
     1381 atccttcgag cctgggctgt gaaggacttt gcccccaact gtcccctcta tgtccagatc
     1441 ctcaagcccg aaaacaagtt tcacgtcaaa tttgctgacc acgtggtatg cgaggaagag
     1501 tgcaagtacg ccatgctggc cctgaactgc atctgcccgg ccacctccac cctcatcacc
     1561 ctgctggtgc acacgtcccg tggccaggaa ggacaggagt ctcccgagca gtggcagcgc
     1621 acgtacggga ggtgctcggg caacgaggtg taccacattc gcatgggtga cagtaaattc
     1681 ttccgggagt atgagggcaa gagcttcacc tacgcagcct tccacgcgca caagaaatat
     1741 ggggtgtgcc tcatcgggct gaagcgtgag gagaacaaga gtatcctgct gaacccagga
     1801 ccacggcaca tcctggctgc ctccgacacc tgcttctata tcaatattac caaggaggag
     1861 aactcagctt tcatcttcaa acaggaggag aagcagaaga gacggggcct tgcagggcag
     1921 gcactatatg aagggccctc ccggctccca gtgcatagca tcatcgcctc tatggggaca
     1981 gtggccatgg acctgcagaa cacagattgc cggccctccc agggtggcag tggcggggac
     2041 ggcacaaagc tgactctgcc caccgagaac ggctctggta gtcgacgccc cagcatcgca
     2101 cccgttctgg agttggcaga cagctcagcc ctgttgccct gcgacctgct gagtgaccag
     2161 tcagaggatg aggtgacacc ctcggacgac gaggggctct ctgtggttga gtacgtgaag
     2221 ggctatcccc ccaactcacc ctacattggc agctccccga ctttatgcca cctcctgcct
     2281 gtgaaagccc ccttctgctg cctgcggttg gacaagggct gcaaacacaa cagctacgag
     2341 gatgccaagg cctatgggtt caagaacaag ctgattattg tctctgctga gacggcaggc
     2401 aacgggctct acaacttcat cgtgcctctg cgcgcctact accggtcccg ccgggagctc
     2461 aaccctatcg tgttgctgct tgacaacaag cctgaccacc acttcctgga ggccatctgt
     2521 tgcttcccca tggtctacta catggaggga tccgtggaca acctggacag cttactgcag
     2581 tgtggcatca tctatgctga caacctggtg gtggtggaca aggagagcac tatgagcgct
     2641 gaagaggact acatggcaga tgccaagacc atcgtcaatg tgcaaaccat gttccggctt
     2701 ttccccagtc tcagcatcac cacggagctc acacaccctt ccaacatgcg gttcatgcag
     2761 ttccgtgcca aggacagcta ctctctggct ctttccaaac ttgaaaagca agaacgggag
     2821 aacggctcca acctggcctt catgttccgc ctgccatttg ctgctggtcg agtatttagt
     2881 atcagcatgt tggatacact gctctaccag tccttcgtga aggactacat gatcaccatc
     2941 accaggctgc tcttgggcct tgatactaca ccaggctccg gctacctctg tgcaatgaag
     3001 gtaaccgagg acgacctgtg gatccgcact tacggccgcc tcttccagaa actctgctcc
     3061 tccagcgccg agatccccat cggcatctac aggaccgagt gccatgtctt ctcggagccc
     3121 catgacgtca gagcccagtc tcagatctcg gtgaacatgg aggactgcga ggatactcgg
     3181 gaggccaagg gaccctgggg cacacgagct gcatctggca gtggcagcac ccatggccgt
     3241 cacgggggca gtgctgaccc agtggagcac ccactactac gtcgcaagag cctgcagtgg
     3301 gcccgcaagc tgagtcgcaa gagcaccaag caggcaggga aggcacctgt ggccacagac
     3361 tggatcaccc agcagcggct cagcctgtac cgacgctcag agcgccagga gctctcagag
     3421 ctggtcaaga accgaatgaa gcacctggga ctgcccacca ctggctatga ggacgtagca
     3481 aatttaacag ccagtgatgt gatgaatcgg gtaaacctgg gatatttgca agatgagatg
     3541 aacgatcatc accagaacac cctttcctat gtactcatca accccccgcc agacacaaga
     3601 ctggaaccca acgacattgt gtacctcatc cgttccgacc ccctggccca tgtggccagc
     3661 agctcccaga gtcggaaaag cagctgcagc aacaagctct catcctgtaa tcctgagacc
     3721 agggatgaga cccagctctg agccccatgc tggggcgaac aggacgacac aggagaagag
     3781 aggagacact gtcacaggct ggcctggccc aagcaggctg cattatgact ttatacagtg
     3841 gctccaagac ttgcccccag aaaggaaatc ctctcagtgg ggccagtcgt gagaccactg
     3901 gaccactctg gaaagtggca tgaagcattt ttttaacctt ttttacaaat ctgaatgatt
     3961 tgcatctctt tacatagctg taccacaact cgtgactggg gtctggctaa agagtgaggc
     4021 aagcagacag gacagggact ggaccctatc ctggatccct ccgtcttgca tggcttcact
     4081 cagcaatttc tgaaggggtg ctggggtgca tgggagaagt aatgacctgg aactggagag
     4141 ttccataggg gactggggag tcctcctgca tggaagccag ctgtgttctc tagccattca
     4201 acatgctgtt ctgatcagtt tctgcatggt tctgggagta caccagggcc tatggggtgc
     4261 ctctccccag aaccaggcaa ggggccaacc agtaaaggtg ccatgccctt taagaggaga
     4321 aaaacagagg ggcttagaaa cctggtcggg agagccccaa cctgagtctg agctctgcag
     4381 cccctgcatg ggggaggggg accacagtgg ctcctccaga agtcctgagc tgtggatgga
     4441 agtcaaaatg ttctttctag ctctcacact tgagctttcc tagccatggc ctttggggac
     4501 atcgaactgg cagatgatgt gtctccacag accatttcac actcaaactc ttgtctctaa
     4561 actcctgatc tgagaagcac aattagctct gggccctggt catgcctcag gtctccaggg
     4621 attgcttcag gcagctgact tcattcttgt ttatggtttg tatgatcaga actggaacct
     4681 tctattaaaa tggatacatc ctgcaggcat gcatggaaca aggcagccca ccatagctct
     4741 ggaatggtgg tggtggtggt ggggagacag ctgaggctca cctgttttag ggttagaatt
     4801 gcctcatgta tccctcaccc cagacttcac agactagctc ccaacctctg ggatggccag
     4861 cagggccttc ccaaaatagt aatgaaatcc tctgtggctg caggtggctg ggcctagaca
     4921 gctgctccaa ggtgtatggg gacgtgatcc ccagaggacc tcttggcaag ctttccattg
     4981 gtttccccct atggtccagt ccagcatgtc ccaaagatag gcacaactca cttaaggtct
     5041 caacgcctgg agatccaccc ttaccccaca cttgggagga aggtgtccct gatgtgacta
     5101 tgtgaacttc ccccttaagt gtcctgaaag gttggggtga agggatggga ccaaagggcc
     5161 tacacttcct cagccagagc agatggaacc tcaggagagc aggagataag ggtcaaggca
     5221 gttgctgtag cttgcttgag agcacttgca cctacgtctc cccttcccat aacctgcctg
     5281 catatccagc cttcctattg ccactctgtg cctgttggaa ccttcttgct cacaaagtat
     5341 gcatgagtcc tggtgaggtg taatgggaca gagctggctc taaggactca gggaagaagt
     5401 ttgggggctt gggtagagaa gcaggtggct cacacagggc ttggctggcc aggacttatc
     5461 taggctagga tctgtcagtg gccatcactc tggagcagga ggggtctgag tctatactca
     5521 cattctctct ccctgctcag ccacctgcca ggctgctgag cccagagaaa caggaaagct
     5581 ggtcttcatc tagatgggac taaatagcct tcaagtccat gggaagagct gccacttctg
     5641 aggcaccttg gggaaagcca cctgctccag gccctgtcag gatgtttact ctcgtgttca
     5701 tctgcagcat ggaaacacat ttcagacttt aacagtgtga ggagggcact cctcccacag
     5761 ctatagacag atacttccag cccagccttt gctagctttt aaagatgccc agccctgggg
     5821 ctggcatttg ggccaggagg atttgtgtgc tcagatacca gtcaccagat caataaaagc
     5881 cacctgcaca cctggaa
//
