LOCUS       NM_028231               2968 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus potassium large conductance calcium-activated channel,
            subfamily M, beta member 2 (Kcnmb2), mRNA.
ACCESSION   NM_028231
VERSION     NM_028231.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Kaiser,M., Wojahn,I., Rudat,C., Ludtke,T.H., Christoffels,V.M.,
            Moon,A., Kispert,A. and Trowe,M.O.
  TITLE     Regulation of otocyst patterning by Tbx2 and Tbx3 is required for
            inner ear morphogenesis in the mouse
  JOURNAL   Development 148 (8) (2021)
   PUBMED   33795231
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Larsen,C.K., Praetorius,H.A., Leipziger,J. and Sorensen,M.V.
  TITLE     Intact colonic KCa1.1 channel activity in KCNMB2 knockout mice
  JOURNAL   Physiol Rep 5 (5) (2017)
   PUBMED   28292889
  REMARK    GeneRIF: we conclude that colonic K(+) secretion is intact or even
            increased in mice lacking the beta2-subunit of KCa1.1 channel
            complex despite no additional compensatory induction of KCa1.1
            beta-subunits.
REFERENCE   3  (bases 1 to 2968)
  AUTHORS   Larsen,C.K., Jensen,I.S., Sorensen,M.V., de Bruijn,P.I., Bleich,M.,
            Praetorius,H.A. and Leipziger,J.
  TITLE     Hyperaldosteronism after decreased renal K+ excretion in KCNMB2
            knockout mice
  JOURNAL   Am J Physiol Renal Physiol 310 (10), F1035-F1046 (2016)
   PUBMED   26962098
REFERENCE   4  (bases 1 to 2968)
  AUTHORS   Whitt,J.P., Montgomery,J.R. and Meredith,A.L.
  TITLE     BK channel inactivation gates daytime excitability in the circadian
            clock
  JOURNAL   Nat Commun 7, 10837 (2016)
   PUBMED   26940770
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2968)
  AUTHORS   Martinez-Espinosa,P.L., Yang,C., Gonzalez-Perez,V., Xia,X.M. and
            Lingle,C.J.
  TITLE     Knockout of the BK beta2 subunit abolishes inactivation of BK
            currents in mouse adrenal chromaffin cells and results in slow-wave
            burst activity
  JOURNAL   J Gen Physiol 144 (4), 275-295 (2014)
   PUBMED   25267913
  REMARK    GeneRIF: compared current properties and cell firing properties of
            wild type and knockout in slices and found that knockout abolished
            inactivation, slowed action potential repolarization, and, during
            constant current injection, decreased firing
REFERENCE   6  (bases 1 to 2968)
  AUTHORS   Shelton,E.L., Ector,G., Galindo,C.L., Hooper,C.W., Brown,N.,
            Wilkerson,I., Pfaltzgraff,E.R., Paria,B.C., Cotton,R.B.,
            Stoller,J.Z. and Reese,J.
  TITLE     Transcriptional profiling reveals ductus arteriosus-specific genes
            that regulate vascular tone
  JOURNAL   Physiol Genomics 46 (13), 457-466 (2014)
   PUBMED   24790087
REFERENCE   7  (bases 1 to 2968)
  AUTHORS   Harrell,M.D., Harbi,S., Hoffman,J.F., Zavadil,J. and Coetzee,W.A.
  TITLE     Large-scale analysis of ion channel gene expression in the mouse
            heart during perinatal development
  JOURNAL   Physiol Genomics 28 (3), 273-283 (2007)
   PUBMED   16985003
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158571.3, AC115930.10 and AC156552.10.
            
            On Oct 4, 2022 this sequence version replaced NM_028231.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058957.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-272               AC158571.3         101880-102151       c
            273-372             AC158571.3         96294-96393         c
            373-495             AC115930.10        4817-4939           c
            496-666             AC156552.10        26948-27118
            667-862             AC156552.10        29948-30143
            863-2968            AC156552.10        46192-48297
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 15.48 cM"
     gene            1..2968
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /note="potassium large conductance calcium-activated
                     channel, subfamily M, beta member 2"
                     /db_xref="GeneID:72413"
                     /db_xref="MGI:MGI:1919663"
     exon            1..272
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            273..372
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            373..495
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             440..1147
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /note="calcium-activated potassium channel, subfamily M
                     subunit beta-2; BKbeta2; slo-beta-2; k(VCA)beta-2; BK
                     channel subunit beta-2; maxi K channel subunit beta-2;
                     charybdotoxin receptor subunit beta-2"
                     /codon_start=1
                     /product="calcium-activated potassium channel subunit
                     beta-2"
                     /protein_id="NP_082507.1"
                     /db_xref="CCDS:CCDS17292.1"
                     /db_xref="GeneID:72413"
                     /db_xref="MGI:MGI:1919663"
                     /translation="MFIWTSGRTSSSYRQDEKRNIYQKIRDHDLLDKRKTVTALKAGE
                     DRAILLGLAMMVCSIMMYFLLGITLLRSYMQSVWTEEAQCALLNVSITETFNCSFSCG
                     PDCWKLSQYPCLQVYVNLTSSGERLLLYHTEETMKINQKCSYIPKCGNNFEESMSLVS
                     VVMENFRRHQHFPCYSDPEGNQKSVILTKLYSSNVLFHSLFWPTCMMAGGVAIVAMVK
                     LTQYLSLLCERIQRINR"
     exon            496..666
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            667..862
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            863..2968
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2530..2535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2555
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /note="major polyA site"
     regulatory      2569..2574
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2587
                     /gene="Kcnmb2"
                     /gene_synonym="2700049B16Rik; 3110031N04Rik"
ORIGIN      
        1 agtctggacc ggcttccaca gagcttcgga gaactaatct taagcatggc tgtctagcgt
       61 gcctgtcact cctattgtcc ttccaaactc ttgcagacat tttgagcgag gagtaaagct
      121 atgagttagc gagggtgtga catcaatggt ccacaaagac agccagcagg aaaggtaaag
      181 atgaggaccg gtggctgtgt ggaggactac acgggatcac tgtcattaaa aattaagcgt
      241 ggcttttgag gaagatgtga caactcctga aggccaaaag tacactccgg tgtctggaag
      301 gaaacttcag tggtgcagcg tgcagactcc aataagatga agcactggaa aagagcatca
      361 ctctgtgaac acgtctttct gccacttccc caggacttcc acagtgagga aaggagctgc
      421 aggaccaaca tcctctaaga tgtttatatg gaccagtggc cggacctctt catcttacag
      481 acaggacgag aaaagaaata tctaccagaa aatcagggac catgacctcc tggacaaaag
      541 gaaaactgtg acagctctga aggctgggga ggaccgggcc atcctgctcg gcctggccat
      601 gatggtgtgc tccatcatga tgtacttcct gctgggaatc acactgctgc gctcctacat
      661 gcagagcgtg tggacagaag aagcccagtg tgccctgctg aatgtgtcaa tcacagaaac
      721 gtttaactgt tccttcagct gtgggcccga ctgttggaag ctctctcagt acccttgcct
      781 gcaggtgtac gtgaacctga catcttccgg ggagaggctc ctcctctacc acacggaaga
      841 gaccatgaag atcaatcaaa agtgctccta tattcctaag tgtggaaaca actttgagga
      901 gtccatgtct ctcgtgagtg tcgtcatgga aaacttcagg agacaccaac acttcccctg
      961 ctattctgac ccagaaggaa accagaagag tgtcatcctg accaaactct acagctccaa
     1021 tgtgctgttc cattctctct tctggccaac ttgtatgatg gctgggggtg tggcaatcgt
     1081 tgctatggtg aaactaactc agtacctctc cctgctttgt gagaggatcc aacggatcaa
     1141 cagataaaag aaaaaaatca tggataagat atttttcttt aaagctcaaa tactgtttta
     1201 tttcgttctt caccaaagaa ccttaagttt gtaatgtgca gtctgttatg ggttccttaa
     1261 ttattctatg tagagcaata atgcaaacgc tgtcctatat gcaaacatga tgtccttatt
     1321 attcaggaga attaactgtt cctcattggt tggttgtttc attatattgt tttgatgctg
     1381 gaactagtct gtcgtctttc caccagaata gggctcagct actcattgct aagtgttgtg
     1441 gggcataggg gcatggaggc actatcccca agaccaggtc tctgttctga gtttccagac
     1501 ttcctgaaga caagattttt cttcatttgt tgtttactga agctacagcc aaaaacacat
     1561 tttttttctt atcctatatt gagccctgta gcaagtgaag gagttgtttc ctgactaaag
     1621 ggtattaaac ttattattat tattattatt atttttacca cagcactgta aggaaggggg
     1681 ggaaacctag ccagcaactt ttctttcatc acttttcatt cacaacttca gatttttgaa
     1741 aataattatc aactctctaa tgtcttccga tcagagtccc ttttccatct atgaccctgc
     1801 tactgtgtgg gagacaaagc tggtgagatc actcccagta aactgactgg gtgttggtag
     1861 gcatgccatt gcaattttgg aaagtctatg ttacaaagat atttatggga aatcttaatt
     1921 ccatttccac tggactatta gaatcaaata ttaatatact aaggactggt gaattggttg
     1981 cagtgggtga gtctgtcagg gccagctatc atgtcagaat attactcttt gagaatttat
     2041 gtttcagtaa acgaatgtat gtagggtctc tctttctgaa aaaaaaaaaa aactgttgcc
     2101 tgttggggtt ttaaatctat gtgtgcagaa tctttgtctc ctctacatat tttatttttc
     2161 tactcactgt tcccttttgg ttgttatatt ttatatgcag aatagacctt tttcctaaca
     2221 catgtttgaa atgaataaca ggtttgaggg aagcctttct tcacattgat gtttactcat
     2281 tctatttggt ggggggaggg gaaagggggt tggttttaaa taaagacatt ctgtctttta
     2341 tttaatattg atatctaagg gagaagacaa acatctatct ttctcatcta tatttaagta
     2401 cccttttgta acatagtatc gatgtttttt aggtaattgc aaaattttac aaatcatttg
     2461 tggaatgaac ggtgaaactt atctgaggaa gcagaatatt gtaattcata gtgtgacttt
     2521 tcataagcaa ataaatccct agggtgtaat caggacttca aatgtgtaat taaatttttt
     2581 aaacccattt gatggcaaga ttttaatact tcttgcattc agataaaccc atgactatcc
     2641 aaacaattct aatttgtatt caaaggtatt ttaagagact gtaaagaaaa tccctttttc
     2701 tgacacttaa acaatacatg ttatgttgta aacatttgga aaacaccaca gaaaatcatt
     2761 tatttttatt ccatctttaa aatgtatttg tcaagataga agaaaatcct ttggaactat
     2821 agggttgaaa tttgcaatct taaatgttcc ctgggattcc catcaactga tgcaaggatg
     2881 aagcatacat gatgacatta tgacatactt tatttttaac ttctttaaga tttttcatta
     2941 aaagttccta gctaagacct ggagccaa
//
