LOCUS       NM_001253364            6194 bp    mRNA    linear   ROD 03-SEP-2024
DEFINITION  Mus musculus potassium large conductance calcium-activated channel,
            subfamily M, alpha member 1 (Kcnma1), transcript variant 7, mRNA.
ACCESSION   NM_001253364
VERSION     NM_001253364.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Kishor Kumar,D.G., Pashupathi,M., Vaidhya,A., Ravi Prakash,G.,
            Bramhane,A., Panigrahi,M., Karikalan,M., Lingaraju,M.C.,
            Manickam,K., Singh,T.U. and Parida,S.
  TITLE     Involvement of ObRb receptor, nitric oxide, and BKCa channel
            signaling pathways in leptin-induced relaxation of pregnant mouse
            uterus
  JOURNAL   Eur J Pharmacol 978, 176796 (2024)
   PUBMED   38945286
  REMARK    GeneRIF: Involvement of ObRb receptor, nitric oxide, and BKCa
            channel signaling pathways in leptin-induced relaxation of pregnant
            mouse uterus.
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Li,Q., Chen,G. and Yan,J.
  TITLE     Transmembrane determinants of voltage-gating differences between BK
            (Slo1) and Slo3 channels
  JOURNAL   Biophys J 123 (14), 2154-2166 (2024)
   PUBMED   38637987
  REMARK    GeneRIF: Transmembrane determinants of voltage-gating differences
            between BK (Slo1) and Slo3 channels.
REFERENCE   3  (bases 1 to 6194)
  AUTHORS   Whelan,S.C.M., Mutchler,S.M., Han,A., Priestley,C., Satlin,L.M.,
            Kleyman,T.R. and Shi,S.
  TITLE     Kcnma1 alternative splicing in mouse kidney: regulation during
            development and by dietary K+ intake
  JOURNAL   Am J Physiol Renal Physiol 327 (1), F49-F60 (2024)
   PUBMED   38779757
  REMARK    GeneRIF: Kcnma1 alternative splicing in mouse kidney: regulation
            during development and by dietary K[+] intake.
REFERENCE   4  (bases 1 to 6194)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 6194)
  AUTHORS   Chen,Y., Markov,N., Gigon,L., Hosseini,A., Yousefi,S., Stojkov,D.
            and Simon,H.U.
  TITLE     The BK Channel Limits the Pro-Inflammatory Activity of Macrophages
  JOURNAL   Cells 13 (4), 322 (2024)
   PUBMED   38391935
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6194)
  AUTHORS   Seaman,M.S., Perarnau,B., Lindahl,K.F., Lemonnier,F.A. and
            Forman,J.
  TITLE     Response to Listeria monocytogenes in mice lacking MHC class Ia
            molecules
  JOURNAL   J Immunol 162 (9), 5429-5436 (1999)
   PUBMED   10228021
REFERENCE   7  (bases 1 to 6194)
  AUTHORS   Xie,J. and McCobb,D.P.
  TITLE     Control of alternative splicing of potassium channels by stress
            hormones
  JOURNAL   Science 280 (5362), 443-446 (1998)
   PUBMED   9545224
REFERENCE   8  (bases 1 to 6194)
  AUTHORS   Ikebe,C., Ohashi,K., Fujimori,T., Bernard,O., Noda,T.,
            Robertson,E.J. and Mizuno,K.
  TITLE     Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization, and
            tissue-specific expression of two alternatively initiated
            transcripts
  JOURNAL   Genomics 46 (3), 504-508 (1997)
   PUBMED   9441759
REFERENCE   9  (bases 1 to 6194)
  AUTHORS   Pallanck,L. and Ganetzky,B.
  TITLE     Cloning and characterization of human and mouse homologs of the
            Drosophila calcium-activated potassium channel gene, slowpoke
  JOURNAL   Hum Mol Genet 3 (8), 1239-1243 (1994)
   PUBMED   7987297
REFERENCE   10 (bases 1 to 6194)
  AUTHORS   Butler,A., Tsunoda,S., McCobb,D.P., Wei,A. and Salkoff,L.
  TITLE     mSlo, a complex mouse gene encoding 'maxi' calcium-activated
            potassium channels
  JOURNAL   Science 261 (5118), 221-224 (1993)
   PUBMED   7687074
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT025624.11, CT010582.12, AC154420.2, AC154433.2 and AC154795.2.
            
            Transcript Variant: This variant (7) differs in the 3' UTR and has
            multiple coding region differences, compared to variant 1. The
            resulting isoform (7) is shorter and has a distinct C-terminus,
            compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HQ221758.1, HQ529473.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-458               CT025624.11        66214-66671
            459-620             CT010582.12        165581-165742       c
            621-682             CT010582.12        12566-12627         c
            683-776             AC154420.2         101450-101543       c
            777-888             AC154420.2         100503-100614       c
            889-964             AC154433.2         163580-163655       c
            965-1040            AC154433.2         150768-150843       c
            1041-1211           AC154433.2         148899-149069       c
            1212-1303           AC154433.2         147274-147365       c
            1304-1414           AC154433.2         130339-130449       c
            1415-1520           AC154433.2         128586-128691       c
            1521-1603           AC154433.2         125723-125805       c
            1604-1673           AC154433.2         121674-121743       c
            1674-1829           AC154433.2         115088-115243       c
            1830-1939           AC154433.2         83650-83759         c
            1940-2008           AC154433.2         72148-72216         c
            2009-2095           AC154433.2         62542-62628         c
            2096-2172           AC154433.2         56499-56575         c
            2173-2346           AC154433.2         46116-46289         c
            2347-2440           AC154795.2         126830-126923       c
            2441-2564           AC154795.2         113708-113831       c
            2565-2789           AC154795.2         108009-108233       c
            2790-2982           AC154795.2         104371-104563       c
            2983-3096           AC154795.2         77515-77628         c
            3097-3227           AC154795.2         71410-71540         c
            3228-3422           AC154795.2         54693-54887         c
            3423-3541           AC154795.2         52219-52337         c
            3542-6194           AC154795.2         47182-49834         c
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 12.92 cM"
     gene            1..6194
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /note="potassium large conductance calcium-activated
                     channel, subfamily M, alpha member 1"
                     /db_xref="GeneID:16531"
                     /db_xref="MGI:MGI:99923"
     exon            1..458
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..3791
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /note="isoform 7 is encoded by transcript variant 7;
                     calcium-activated potassium channel subunit alpha-1; slo
                     homolog; maxi K channel; slowpoke homolog;
                     calcium-activated potassium channel, subfamily M subunit
                     alpha-1; BK channel alpha subunit"
                     /codon_start=1
                     /product="calcium-activated potassium channel subunit
                     alpha-1 isoform 7"
                     /protein_id="NP_001240293.1"
                     /db_xref="GeneID:16531"
                     /db_xref="MGI:MGI:99923"
                     /translation="MANGGGGGGGSSGGGGGGGGGSGLRMSSNIHANHLSLDASSSSS
                     SSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGL
                     FIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMT
                     SVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQID
                     MAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRA
                     LRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNN
                     QALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIG
                     NRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNL
                     ELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMR
                     VISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQ
                     GLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFV
                     KLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHD
                     DVTDPKRIKKCGCRRPKMSIYKRMRRACCFDCGRSERDCSCMSGRVRGNVDTLERTFP
                     LSSVSVNDCSTSFRAFEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQ
                     IDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIG
                     LRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRA
                     VNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPP
                     GMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQ
                     PFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYST
                     PQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLS
                     TPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSS
                     SVHSIPSTANRPNRPKSRESRDKQKYVQEERL"
     exon            459..620
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..682
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            683..776
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            777..888
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            889..964
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1040
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1211
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1303
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1414
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1520
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1603
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1673
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1829
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1939
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2008
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2095
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2172
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2346
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2440
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2564
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2789
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2790..2982
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            2983..3096
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3227
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3228..3422
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3423..3541
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..6194
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4741..4746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /note="hexamer: AATAAA"
     polyA_site      4762
                     /gene="Kcnma1"
                     /gene_synonym="5730414M22Rik; BKCa; BKCA alpha;
                     k(VCA)alpha; KCa1.1; MaxiK; mSlo; mSlo1; Slo; slo-alpha;
                     Slo1"
                     /note="major polyA site"
ORIGIN      
        1 gctgttgatg ggtgttcggg gtggcgctgg cggcgggagg agctccctcg agcccctgcg
       61 cctgccgccc attgctagct atggcaaacg gtggcggcgg cggcggcggc agcagcggcg
      121 gcggcggcgg cggcggcgga ggcagcggtc ttagaatgag cagcaatatc cacgcgaacc
      181 atctcagcct agacgcgtcc tcctcctcct cttcctcctc ctcttcttct tcttcctcct
      241 cctcttcctc ctcgtcctcg gtccacgagc ccaagatgga tgcgctcatc ataccggtga
      301 ccatggaggt gccgtgcgac agccggggcc aacgcatgtg gtgggctttc ttggcctcct
      361 ccatggtgac tttcttcggg ggcctcttca tcatcttgct ctggcggacg ctcaagtacc
      421 tgtggaccgt ttgctgccac tgcgggggca agacgaagga ggcccagaag ataaacaatg
      481 gctccagcca ggcagatggt actctcaagc cagtggacga aaaagaggag gtggtggcag
      541 ccgaggtcgg ctggatgaca tctgtgaagg actgggcagg ggtgatgata tccgcccaga
      601 cactgactgg cagagtcctg gttgtgttag tctttgctct cagcattggt gccctcgtaa
      661 tatacttcat agactcgtca aacccaatag aatcctgcca gaatttctac aaagatttca
      721 cattacagat cgacatggct ttcaacgtgt tcttcctcct ctactttggc ttgcggttta
      781 ttgcagccaa cgataagctg tggttctggc tggaagtgaa ttcagtagta gatttcttca
      841 cagtccctcc tgtgtttgtg tctgtgtact taaacagaag ttggcttggc ttgagatttt
      901 taagagctct cagactgata cagttttcag agattttgca gtttctgaat atccttaaaa
      961 caagtaactc catcaagctg gtgaatctgc tctccatatt tatcagcacg tggctgactg
     1021 cagctggatt catccacttg gtggagaatt caggggaccc atgggaaaat ttccaaaaca
     1081 accaggcact tacgtactgg gaatgtgtct acttactcat ggtcacaatg tctacagtgg
     1141 gttatgggga cgtttatgca aaaaccacac ttggacgcct cttcatggtc ttcttcatcc
     1201 tcgggggact ggccatgttt gccagctacg tccctgaaat catagagtta ataggaaacc
     1261 gcaagaaata cgggggctcc tatagcgcgg ttagtggaag aaagcacatt gtagtctgtg
     1321 gacacattac tctggagagt gtctctaact tcctgaagga ctttctgcac aaggaccggg
     1381 atgatgtcaa cgtggagatt gtctttcttc acaacatctc ccctaacctt gagcttgagg
     1441 ctctgttcaa acggcatttc actcaggtgg agttttatca gggctctgtc ctcaatccac
     1501 atgatcttgc cagagtcaag atagagtcag cagatgcatg cctgatcctt gccaataagt
     1561 attgcgctga cccggatgca gaagatgcct ccaacatcat gagagtgatc tccatcaaaa
     1621 actaccaccc aaagatcagg atcatcactc agatgctgca gtatcacaac aaggcccatc
     1681 tgctcaacat ccccagctgg aactggaaag agggtgatga cgcaatatgc cttgcagagc
     1741 tcaagttggg tttcatagcc cagagctgtc tggctcaagg cctctccaca atgcttgcca
     1801 atctcttctc tatgaggtca ttcataaaga ttgaggaaga cacatggcag aaatactact
     1861 tggaaggagt ctccaatgaa atgtacacag aatatctctc cagtgccttc gtgggtctgt
     1921 ccttccctac tgtttgtgag ctgtgttttg tgaagcttaa gctcctgatg atagccattg
     1981 agtacaagtc tgccaacaga gagagccgaa tattaattaa ccctgggaac caccttaaga
     2041 tccaagaagg tactttagga tttttcatcg caagtgatgc caaagaagtt aaaagggcat
     2101 ttttttactg caaggcctgt catgatgacg tcacagatcc caaaagaatt aaaaaatgtg
     2161 gctgcaggcg gcccaagatg tccatctaca agagaatgag acgagcatgt tgttttgatt
     2221 gcggacgttc tgagcgtgac tgctcgtgca tgtcaggccg tgtgcgtggt aacgtggaca
     2281 cccttgagag aaccttcccg ctttcttctg tctctgttaa tgattgctcc accagtttcc
     2341 gtgcctttga agatgagcag ccgccaaccc tgtcaccaaa aaaaaaacaa cgtaatgggg
     2401 gcatgaggaa ctcgcccaac acctccccga agctgatgag gcatgacccc ttgttaattc
     2461 ctggcaatga tcagattgac aacatggact ccaatgtgaa aaagtacgac tccactggaa
     2521 tgtttcactg gtgtgcaccc aaggagattg agaaagtcat cttgactcga agtgaagctg
     2581 ccatgactgt cctgagtggc catgtcgtag tctgcatctt tggggatgtc agctcagccc
     2641 tgattggcct ccggaacctg gtgatgccac ttcgtgctag caactttcac tatcatgagc
     2701 tcaaacacat tgtgtttgtg ggctccattg agtacctcaa gagggagtgg gaaacactgc
     2761 acaacttccc gaaagtgtcc atattgcctg gtacaccatt aagtcgggct gatttaaggg
     2821 ctgtcaacat caacctctgt gacatgtgcg ttatcctgtc agccaatcag aataatattg
     2881 atgatacttc gcttcaggac aaggaatgca tcttggcgtc actcaacatc aaatctatgc
     2941 agtttgatga cagcatcggg gtcttgcagg ctaattccca aggattcaca cctcctggaa
     3001 tggacagatc atcacccgac aacagcccag tgcacgggat gttacgccag ccgtccatca
     3061 caactggggt caacattccc atcatcacgg aactcgtgaa tgataccaat gttcagtttt
     3121 tggaccaaga cgatgacgat gaccctgaca cagagctgta cctcacacag ccctttgctt
     3181 gtgggacagc atttgccgtc agcgtcctgg actcactcat gagcgcgaca tacttcaatg
     3241 acaatatcct caccctaata cggaccctgg tgacaggagg agccacacca gagctcgagg
     3301 ctctaatagc tgaggagaat gcacttcgag gaggctacag cactccgcag acattggcca
     3361 acagggaccg ttgccgagtg gcccagttag ccctgttaga tggtcccttt gcagacttag
     3421 gggatggtgg ttgttatggt gatctgttct gcaaagctct gaaaacatat aatatgcttt
     3481 gttttggaat ttaccggctg agagatgccc acctcagcac ccccagccag tgtacaaaaa
     3541 ggtacgtcat caccaatcct ccctacgagt ttgagctcgt accaacagac ctgatcttct
     3601 gcctgatgca gtttgaccac aacgctggcc aatcccgggc cagtctgtct cattcctccc
     3661 actcctcaca gtcgtccagt aagaagagct cctccgtcca ctccatcccg tccacagcaa
     3721 atcggccgaa ccggcccaag tccagggagt cccgcgacaa acagaagtac gttcaggaag
     3781 agcggctttg atatgtgtag ccaccggcac ccgtgtgtga aactgtatct gctactcatt
     3841 tccccagttg gtattccaac agagtaactt tcctgctccc tatagtcccc tctttttttt
     3901 tcttttcttt tctttttttt ttttacacat tttgcatatg tatgatagtg tgcatgtggt
     3961 tgtcattttt atttcaccac cataaaaccc ttgagcgcaa cagcaaataa gcagacggac
     4021 caaaagttat ttatgcttcc agggggaaag taacgtaaag gcatcctcca gacataaata
     4081 gctcactgca gggatgagtt cacaggttag aagggagcac ctgagagtta ggcaacggag
     4141 gtttgcctga tttatcagga tcatcaggat gctttgggtt ttgcgttttc attttttgtt
     4201 tgttttctct ctctctgttt ttatacacaa acaataagtt agcacacagt cgtttgaaac
     4261 aagcaaccaa aaagcaatga aaacgcattg atcgtttcca ctctgggctg aggtatcgca
     4321 aaacatttga aaagctttcc aagtgtgcgc aaaagatgat caagtatctg cttgttgtga
     4381 gagagcgtca gagacttttt ttttttaacg tattcaccaa agcctttttg tctcggagct
     4441 agcgtctttc aggtgtttga gtgaatgttt ctaactttaa tgcagagaga aaaacaggtt
     4501 tccactcctg gatgtatcaa atcttagtca tttcatttac ctgaaaaaag gcttgatcac
     4561 atattaaact gttattctgt ctccttatgc tgccataggg atagctagtt tttttctttc
     4621 acttttgaca tttgggatga aaagccatat gtatcataaa tatcagatgt aagtcattaa
     4681 gaactgcctt cctgggactt ttacatcctt taaaaggtga atcacttacc ttatgtacag
     4741 aataaataac actcagaaaa gagcaagtat ttttccatgc attctcaagg gaccttttta
     4801 ctcccctttg tttgactaac gagggccccg atgccaagta caattcaggc tggggcaatg
     4861 gtagagcagg aaggagacac ccagtgatag ataatggttt cttgtaggag ctgacgtgct
     4921 caataaatca gaatgtagga ggatagagcc acagttgatt caagaaagac aaaagctagc
     4981 gaccacattt aaattgctgg cacagctgca tgatgctctg tcccaaagca gatggctggc
     5041 aggaaaggtc taacataggt aaccctctta aatgtacagc caattcctat aggtagatga
     5101 gagacagggg agagagcttc tcccttcatt tcaagattgg aaggaggcgg ctgcaacagt
     5161 aagccaaaca cattgcagta gttaatttct ttcttttctt tccttttttt ttttctttaa
     5221 aaaaaaaaaa aacaaacctg ttattcatca agggagggaa gatagataga taccttttcc
     5281 ccaatatgca actatcagat gttttatgca tgcaaattat gttttaagaa catagttctg
     5341 aagaattggc caactttacc ataaccaaat tctggtatgg gtagactagc cttgggctca
     5401 tgtgaacgta tttgggatat ttatttagaa atccactgat agaacagggg gtagaaagga
     5461 ggggggaaat ctgtcatatc tttagcatct ctttttaaaa ggtgttttta tggtgaatgt
     5521 tttgggttta gatttttgat cccctgtccc ctccagcatg atggttttgg agatttgctt
     5581 gctgtgtgac tgacaagcac ttttactgac aaaaaatggt gaggctctgt cagaacctcc
     5641 acctcacccc acaccaaaga caggggcagt gcagtattcc aaccagcaat gggtgggcag
     5701 tcaatgtata catgtaaatt caaaagtcct gtgagtggaa gtattttttt tcaaattatt
     5761 tttgtcctta tgtatttgat ttatatatga catctagtta tctctttata tataattata
     5821 tatataaacc atatatatac atatatatat aaatatatat atttatatat atgtatacat
     5881 gtcccctagt tttggatcat gagagatctt catgcattct ttgcaaagga ggttatgagg
     5941 ttacgccttc agaacattga taactataag aatgtgccag ttaaagtgct caacaggaaa
     6001 tgacagtttc aaagcattgt aaaactcaca tagcttactt ctctctctaa agtgcaacaa
     6061 ggatgactag aatgggccaa ggtatgacaa ttaatggttc tgcatgacct agccactgct
     6121 gggggttttc ttctataacg ttgtccttgt gaaacttttg tggaattaaa aaaaaaaagg
     6181 agttacaaat ttta
//
