LOCUS       NM_001033525            4089 bp    mRNA    linear   ROD 09-JAN-2024
DEFINITION  Mus musculus potassium inwardly-rectifying channel, subfamily K,
            member 6 (Kcnk6), mRNA.
ACCESSION   NM_001033525 XM_355877 XM_908552
VERSION     NM_001033525.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Zhi Y, Wu X, Chen Y, Chen X, Chen X, Luo H, Yi X, Lin X, Ma L, Chen
            Y, Cao Y, Li F and Zhou P.
  TITLE     A novel TWIK2 channel inhibitor binds at the bottom of the
            selectivity filter and protects against LPS-induced experimental
            endotoxemia in vivo
  JOURNAL   Biochem Pharmacol 218, 115894 (2023)
   PUBMED   37898389
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Huang LS, Anas M, Xu J, Zhou B, Toth PT, Krishnan Y, Di A and Malik
            AB.
  TITLE     Endosomal trafficking of two-pore K+ efflux channel TWIK2 to
            plasmalemma mediates NLRP3 inflammasome activation and inflammatory
            injury
  JOURNAL   Elife 12, e83842 (2023)
   PUBMED   37158595
  REMARK    GeneRIF: Endosomal trafficking of two-pore K[+] efflux channel
            TWIK2 to plasmalemma mediates NLRP3 inflammasome activation and
            inflammatory injury.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4089)
  AUTHORS   Kitagawa MG, Reynolds JO, Durgan D, Rodney G, Karmouty-Quintana H,
            Bryan R and Pandit LM.
  TITLE     Twik-2-/- mouse demonstrates pulmonary vascular heterogeneity in
            intracellular pathways for vasocontractility
  JOURNAL   Physiol Rep 7 (1), e13950 (2019)
   PUBMED   30632293
  REMARK    GeneRIF: Twik-2-/- mice have enhanced vasocontractility in the
            distal pulmonary vasculature.
REFERENCE   4  (bases 1 to 4089)
  AUTHORS   Di A, Xiong S, Ye Z, Malireddi RKS, Kometani S, Zhong M, Mittal M,
            Hong Z, Kanneganti TD, Rehman J and Malik AB.
  TITLE     The TWIK2 Potassium Efflux Channel in Macrophages Mediates NLRP3
            Inflammasome-Induced Inflammation
  JOURNAL   Immunity 49 (1), 56-65 (2018)
   PUBMED   29958799
  REMARK    GeneRIF: Deletion of Kcnk6 (encoding two-pore domain K(+) channel
            TWIK2) prevents NLR Family, Pyrin Domain-Containing 3 Protein
            (NLRP3) inflammasome activation in macrophages and suppressed
            sepsis-induced lung inflammation. Adoptive transfer of Kcnk6(-/-)
            macrophages into mouse airways after macrophage depletion also
            prevents inflammatory lung injury.
REFERENCE   5  (bases 1 to 4089)
  AUTHORS   Madry C, Kyrargyri V, Arancibia-Carcamo IL, Jolivet R, Kohsaka S,
            Bryan RM and Attwell D.
  TITLE     Microglial Ramification, Surveillance, and Interleukin-1beta
            Release Are Regulated by the Two-Pore Domain K+ Channel THIK-1
  JOURNAL   Neuron 97 (2), 299-312 (2018)
   PUBMED   29290552
REFERENCE   6  (bases 1 to 4089)
  AUTHORS   Pandit LM, Lloyd EE, Reynolds JO, Lawrence WS, Reynolds C, Wehrens
            XH and Bryan RM.
  TITLE     TWIK-2 channel deficiency leads to pulmonary hypertension through a
            rho-kinase-mediated process
  JOURNAL   Hypertension 64 (6), 1260-1265 (2014)
   PUBMED   25245387
  REMARK    GeneRIF: mice deficient in the TWIK-2 channel develop pulmonary
            hypertension between 8 and 20 weeks of age through a mechanism
            involving Rho-kinase.
REFERENCE   7  (bases 1 to 4089)
  AUTHORS   Lloyd EE, Pandit LM, Crossland RF, Marrelli SP and Bryan RM Jr.
  TITLE     Endothelium-dependent relaxations in the aorta from K(2p)6.1
            knockout mice
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 305 (1), R60-R67 (2013)
   PUBMED   23637138
REFERENCE   8  (bases 1 to 4089)
  AUTHORS   Lloyd EE, Crossland RF, Phillips SC, Marrelli SP, Reddy AK, Taffet
            GE, Hartley CJ and Bryan RM Jr.
  TITLE     Disruption of K(2P)6.1 produces vascular dysfunction and
            hypertension in mice
  JOURNAL   Hypertension 58 (4), 672-678 (2011)
   PUBMED   21876070
REFERENCE   9  (bases 1 to 4089)
  AUTHORS   Harrell MD, Harbi S, Hoffman JF, Zavadil J and Coetzee WA.
  TITLE     Large-scale analysis of ion channel gene expression in the mouse
            heart during perinatal development
  JOURNAL   Physiol Genomics 28 (3), 273-283 (2007)
   PUBMED   16985003
REFERENCE   10 (bases 1 to 4089)
  AUTHORS   Czirjak G and Enyedi P.
  TITLE     Zinc and mercuric ions distinguish TRESK from the other
            two-pore-domain K+ channels
  JOURNAL   Mol Pharmacol 69 (3), 1024-1032 (2006)
   PUBMED   16354767
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132480.1 and AK163847.1.
            
            On Mar 19, 2008 this sequence version replaced NM_001033525.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132480.1, AK036882.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-673               AK132480.1         2-674
            674-1943            AK163847.1         523-1792
            1944-3399           AK132480.1         1947-3402
            3400-3490           AK163847.1         3249-3339
            3491-3751           AK132480.1         3494-3754
            3752-4089           AK132480.1         3756-4093
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 16.94 cM"
     gene            1..4089
                     /gene="Kcnk6"
                     /gene_synonym="D7Ertd764e; Toss; Twik2"
                     /note="potassium inwardly-rectifying channel, subfamily K,
                     member 6"
                     /db_xref="GeneID:52150"
                     /db_xref="MGI:MGI:1891291"
     exon            1..361
                     /gene="Kcnk6"
                     /gene_synonym="D7Ertd764e; Toss; Twik2"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..981
                     /gene="Kcnk6"
                     /gene_synonym="D7Ertd764e; Toss; Twik2"
                     /note="TWIK-2 two-pore-domain K+ channel"
                     /codon_start=1
                     /product="potassium channel subfamily K member 6"
                     /protein_id="NP_001028697.2"
                     /db_xref="CCDS:CCDS21068.1"
                     /db_xref="GeneID:52150"
                     /db_xref="MGI:MGI:1891291"
                     /translation="MRRGALLASALAAYAGYLALGALLVARLERPHEARLRAELGTLR
                     EQLLQHSPCVAAHALDAFVERVLAAGRLGRAALANASGAANASDPAWDFASALFFAST
                     LVTTVGYGYTTPLTDAGKAFSIVFALLGVPITMLLLTASAQRLSLLLTHAPLSWLSLH
                     WGWPPQRAARWHLVALLMVIVAIFFLVPAAVFAYLEEAWSFLDAFYFCFISLSTIGLG
                     DYVPGEAPGQPYRALYKVLVTAYLFLGLVAMVLVLQTFRRVSDLHGLTELILLPDPDP
                     ASLSQDEDDQVAVLDARTDLHQHLSAASHADYASIPR"
     exon            362..757
                     /gene="Kcnk6"
                     /gene_synonym="D7Ertd764e; Toss; Twik2"
                     /inference="alignment:Splign:2.1.0"
     exon            758..4089
                     /gene="Kcnk6"
                     /gene_synonym="D7Ertd764e; Toss; Twik2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4067..4072
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnk6"
                     /gene_synonym="D7Ertd764e; Toss; Twik2"
                     /note="putative"
ORIGIN      
        1 gggggccacg tcagcgggtg tcggggctgt acgggcacca tgcggcgggg cgcgctcctg
       61 gctagcgccc tggccgccta cgccgggtac ctggcgctgg gcgcgctgct tgtggcgcgg
      121 ctggagcggc ctcacgaagc ccggctgcgg gcagaactgg ggaccctgcg ggagcagctg
      181 ctacagcaca gcccgtgcgt ggcggctcac gccctggatg ccttcgtgga gcgggtgctg
      241 gcggccggac ggctggggcg cgcagcgctc gccaacgcct cgggagctgc caacgcttcg
      301 gaccctgcgt gggacttcgc ctcggcgctc ttcttcgcca gcacgcttgt caccaccgtg
      361 ggctatggct acacgacccc actgacagat gctggcaaag ccttctccat cgtctttgca
      421 ctcctgggcg tgcctatcac catgctactt ctgactgcct cagcccagcg cctgtcactg
      481 ctgctcaccc acgcacccct gtcctggctg agcctgcact ggggctggcc cccgcagcgg
      541 gcagcccgct ggcacctggt ggccctgctg atggtcatag tggctatatt cttcctggta
      601 ccggccgcgg tcttcgccta ccttgaagag gcctggagtt ttctggatgc tttctatttc
      661 tgcttcatct ctctgtccac cattggccta ggggactacg tgcctgggga agccccaggc
      721 cagccttacc gggccctcta caaggtgctt gtcacagcgt acctcttcct cggcctggtt
      781 gccatggtgc tcgtgttgca gactttccgc cgtgtttccg acctccatgg cctcacagaa
      841 ctcatcttgc tgcccgatcc ggaccctgcc agcctcagcc aggatgagga tgatcaggtg
      901 gccgttctgg atgcccggac agatctacat cagcacctct ctgctgcctc ccacgctgac
      961 tacgcctcca tccccaggta gctgtcgacc tggacacacc tgggcagggg ctttgagccc
     1021 catgtgacta gatcaactgg aatgcccaga cacactctcc tgtcttttca cagagctcca
     1081 ctgggaggtg aggagtagtc cctctgctcc tatgtcctct gctcctcagg gcactgacac
     1141 agtcattttc tcctccttcc ttttctccct tttcttctca gactcagacc acgtgggctt
     1201 tctgactttg ttggtgagag tagtggttta tacaattttt ttgttttgtt ttgttcttgt
     1261 tgtttttcta tgtctcgctc tcttgcatag ctcatccggc acacctagtt cttctgggct
     1321 ctgggcctgg actccacttt agcctctgta ttagtggctt tgtcttggcc aaatgatttc
     1381 cccattctaa acctttatat cccagccata agatgcaaag aatgttcaaa ctcatccaca
     1441 gaccttcact tctgccatgc ttttctgtgc taggcacaca gtagtagatg tggcttgggc
     1501 tctgctctta ttggggtgac cagccagtga ggtaaactga ctgacttgct gtcccttgat
     1561 ggtgacaaac tccagtgggc actggaagag gcagagtggt caggactgag gagtggacct
     1621 gaaatgtcca ggtgggcttt cctggaaaac gggacaagaa ctaacccaag acctgccagt
     1681 caagagaaga aattctgcct tcttctcagg tgtctatggg ccgtagggcc tgtgggaaga
     1741 ggcctgcaag gggccagcat ctccctcaac gacttaacag gcaaggaact gacccaggat
     1801 atgagcccag atccaagtgg cgtgagaggg gtaacttagc ctggtcctat ggtgatgctc
     1861 tgtgtaccgg ggcactgact gatggccttt ctgggcatgc cctgtaactg ggccatattt
     1921 tgccaatgca tttggacagt cactctacct ttgggactct gtgtctgcta aatggggaat
     1981 cataatgcta ctccaaaagt tgacatcaac caggtgcagt actgggtacc agtagcctga
     2041 gctgaagcag gaggcttgag agttcaaagg caggatggga tacacagcag gagccaggtg
     2101 ataaaataag ctaagcaata taataataat aataataata ataataataa taataataat
     2161 aataaaatca caggaatata gtgccaagct aaaggggagt aggctacaga aggatataca
     2221 gtccttgggg ttggaaatta cttatacaat tgtatcactt aaggattttg actgcaactg
     2281 acaaaagcca gactgaaaca acctgattgt tcaattgtaa ctggacagaa cccgggtggg
     2341 gagagactga gcttgatgct ctgcatggct acagaagggg tggtggccgt gggttcagcc
     2401 tctcttctcc ccatagcaga cttagctctc atatgtgttc tcctcctggt gtctaatggt
     2461 tgccacagac ccaggcatcg aaaccagacg tgttatttct agtgcaagca atagaaactt
     2521 caagcagtat tctgagaact ttaaggaagg ctggctcagt accaaaccac tgtagaaagt
     2581 cacacggggg aactgggtgt gttggcatac agctacccta gcactgtgga gggtgtgcct
     2641 ggagagtcct gaggctaggc tagcctgggc aaccgataat gacaccacaa aactccaaag
     2701 ggagctagga atgttcacag tggtggaaca cgtctgcctc aggtgctaaa gaaaacaatt
     2761 tacaattata ggtgctggga agatggctta gccaggcagg ctcaaaaacc tgagtttctc
     2821 cccagtccct acatagaaaa tgctagatgt catgtcatgg tgctgccctt taatcccagc
     2881 atctggaggc agaagctggt agaatttctg cgagttcaag gccagcatgg tctacttagt
     2941 gagtttgagg acaattaggg ctacatagta agaccctgca tccacaaaat aactaaaaac
     3001 taaaaatagt gtgacaagtg attaggaaaa aagtagatat taaactctag cttccatgtg
     3061 taaactcaca cacacttaaa agtctgaggc agcaaaatat ttttttggcg gggggctggt
     3121 ttcaagacag ggtttctcta tgtagccctg gctatcctgg aactcacttt gtagaccagg
     3181 ctggccttga actcagaaat ctgcctgcct ctgcttccta agtgctggga ttaaaggtat
     3241 gcaccaccac acccggcttc ataatgagtt atttctctac cagtgaaatg agtcagttca
     3301 agccagcttg gattatattg aaggcaggtt tattgggaaa ctggccttgg ttgagttcac
     3361 tggccccaag gattgagccc aggggaattg ccattggagg tggtatgggg agaaaagaca
     3421 catgtgcaca catacacagg gggaaagaca gatgtctgaa ttatataggg aggagcctgg
     3481 gggggggggg gagaacagcc cagcccctga gctaaaaagt tcaagcttgg ggtcaggata
     3541 tgtcaggtag ggactgaggg atgctgggag aacctggagg ccaggtctgc tctgatatgt
     3601 aaaatgcata cctcaatccc ttgtcccagg gtccaaaacc aaagtaccca aagtacaatt
     3661 gatttctcaa gtctttatct gcatataggc tatgcacgaa ctagcaatat gtggatgtgc
     3721 atatgtgtga gtcttccacc ctacacaaag gttaccagca atgggaagtg atgactattc
     3781 ctggttctga aagcagaact ctgtgagttt gaacccttgt ccttgtattt cacttgtgcc
     3841 tcagattgct tatttatgaa agggtatttg tacagcacta cctcgtggac tggaacaccc
     3901 agccgtctaa tgcttgtaac tcttacccta cagcaccatc agggagtcaa taaagtgcct
     3961 gacccgtccc tcgatctgaa ctctgttctt agcaaggaag gtcacgtaaa atagaaatgc
     4021 gtgacttttg ggatagcatt ggaaatgtaa atgaagaaaa tacctaatta aaaaaaaaaa
     4081 gaaatgcgt
//
