LOCUS       NM_001159850            3405 bp    mRNA    linear   ROD 20-OCT-2024
DEFINITION  Mus musculus potassium channel, subfamily K, member 2 (Kcnk2),
            transcript variant 1, mRNA.
ACCESSION   NM_001159850
VERSION     NM_001159850.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Xie,A.X., Iguchi,N. and Malykhina,A.P.
  TITLE     Long-term follow-up of TREK-1 KO mice reveals the development of
            bladder hypertrophy and impaired bladder smooth muscle
            contractility with age
  JOURNAL   Am J Physiol Renal Physiol 326 (6), F957-F970 (2024)
   PUBMED   38601986
  REMARK    GeneRIF: Long-term follow-up of TREK-1 KO mice reveals the
            development of bladder hypertrophy and impaired bladder smooth
            muscle contractility with age.
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Yu,H., Wang,X., Tian,R., Li,X., Xu,C., Fei,J., Li,T. and Yin,Z.
  TITLE     Myometrium infection decreases TREK1 through NHE1 and increases
            contraction in pregnant mice
  JOURNAL   Am J Physiol Cell Physiol 326 (4), C1106-C1119 (2024)
   PUBMED   38344766
  REMARK    GeneRIF: Myometrium infection decreases TREK1 through NHE1 and
            increases contraction in pregnant mice.
REFERENCE   3  (bases 1 to 3405)
  AUTHORS   Immanuel,C.N., Teng,B., Dong,B.E., Gordon,E.M., Luellen,C.,
            Lopez,B., Harding,J., Cormier,S.A., Fitzpatrick,E.A.,
            Schwingshackl,A. and Waters,C.M.
  TITLE     Two-pore potassium channel TREK-1 (K2P2.1) regulates NLRP3
            inflammasome activity in macrophages
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 326 (3), L367-L376 (2024)
   PUBMED   38252657
  REMARK    GeneRIF: Two-pore potassium channel TREK-1 (K2P2.1) regulates NLRP3
            inflammasome activity in macrophages.
REFERENCE   4  (bases 1 to 3405)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 3405)
  AUTHORS   Spencer,K.A., Woods,C.B., Worstman,H.M., Johnson,S.C.,
            Ramirez,J.M., Morgan,P.G. and Sedensky,M.M.
  TITLE     TREK-1 and TREK-2 Knockout Mice Are Not Resistant to Halothane or
            Isoflurane
  JOURNAL   Anesthesiology 139 (1), 63-76 (2023)
   PUBMED   37027798
REFERENCE   6  (bases 1 to 3405)
  AUTHORS   Bockenhauer,D., Nimmakayalu,M.A., Ward,D.C., Goldstein,S.A. and
            Gallagher,P.G.
  TITLE     Genomic organization and chromosomal localization of the murine 2 P
            domain potassium channel gene Kcnk8: conservation of gene structure
            in 2 P domain potassium channels
  JOURNAL   Gene 261 (2), 365-372 (2000)
   PUBMED   11167025
REFERENCE   7  (bases 1 to 3405)
  AUTHORS   Meadows,H.J., Benham,C.D., Cairns,W., Gloger,I., Jennings,C.,
            Medhurst,A.D., Murdock,P. and Chapman,C.G.
  TITLE     Cloning, localisation and functional expression of the human
            orthologue of the TREK-1 potassium channel
  JOURNAL   Pflugers Arch 439 (6), 714-722 (2000)
   PUBMED   10784345
REFERENCE   8  (bases 1 to 3405)
  AUTHORS   Patel,A.J., Honore,E., Lesage,F., Fink,M., Romey,G. and
            Lazdunski,M.
  TITLE     Inhalational anesthetics activate two-pore-domain background K+
            channels
  JOURNAL   Nat Neurosci 2 (5), 422-426 (1999)
   PUBMED   10321245
REFERENCE   9  (bases 1 to 3405)
  AUTHORS   Kim,D., Fujita,A., Horio,Y. and Kurachi,Y.
  TITLE     Cloning and functional expression of a novel cardiac two-pore
            background K+ channel (cTBAK-1)
  JOURNAL   Circ Res 82 (4), 513-518 (1998)
   PUBMED   9506712
REFERENCE   10 (bases 1 to 3405)
  AUTHORS   Fink,M., Duprat,F., Lesage,F., Reyes,R., Romey,G., Heurteaux,C. and
            Lazdunski,M.
  TITLE     Cloning, functional expression and brain localization of a novel
            unconventional outward rectifier K+ channel
  JOURNAL   EMBO J 15 (24), 6854-6862 (1996)
   PUBMED   9003761
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124527.5 and AC121882.3.
            
            On Mar 23, 2022 this sequence version replaced NM_001159850.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.1275983.1,
                                           SRR7345562.897036.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-310               AC124527.5         181838-182147       c
            311-621             AC124527.5         178580-178890       c
            622-739             AC124527.5         134389-134506       c
            740-900             AC124527.5         97533-97693         c
            901-1087            AC124527.5         95363-95549         c
            1088-1227           AC124527.5         81682-81821         c
            1228-3107           AC124527.5         47023-48902         c
            3108-3405           AC121882.3         173079-173376       c
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 94.22 cM"
     gene            1..3405
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /note="potassium channel, subfamily K, member 2"
                     /db_xref="GeneID:16526"
                     /db_xref="MGI:MGI:109366"
     exon            1..310
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..1545
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     TREK-1 K(+) channel subunit; two pore potassium channel
                     TPKC1; outward rectifying potassium channel protein
                     TREK-1"
                     /codon_start=1
                     /product="potassium channel subfamily K member 2 isoform
                     1"
                     /protein_id="NP_001153322.1"
                     /db_xref="CCDS:CCDS48480.1"
                     /db_xref="GeneID:16526"
                     /db_xref="MGI:MGI:109366"
                     /translation="MLASASRERPGYTAGVAAPDLLDPKSAAQNSKPRLSFSSKPTVL
                     ASRVESDSAINVMKWKTVSTIFLVVVLYLIIGATVFKALEQPQEISQRTTIVIQKQTF
                     IAQHACVNSTELDELIQQIVAAINAGIIPLGNSSNQVSHWDLGSSFFFAGTVITTIGF
                     GNISPRTEGGKIFCIIYALLGIPLFGFLLAGVGDQLGTIFGKGIAKVEDTFIKWNVSQ
                     TKIRIISTIIFILFGCVLFVALPAVIFKHIEGWSALDAIYFVVITLTTIGFGDYVAGG
                     SDIEYLDFYKPVVWFWILVGLAYFAAVLSMIGDWLRVISKKTKEEVGEFRAHAAEWTA
                     NVTAEFKETRRRLSVEIYDKFQRATSVKRKLSAELAGNHNQELTPCRRTLSVNHLTSE
                     REVLPPLLKAESIYLNGLTPHCAGEDIAVIENMK"
     exon            311..621
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            622..739
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            740..900
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1087
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1227
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..3405
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3383..3388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /note="hexamer: AATAAA"
     polyA_site      3405
                     /gene="Kcnk2"
                     /gene_synonym="A430027H14Rik; K2P2.1; TREK-1"
                     /note="major polyA site"
ORIGIN      
        1 gaaaatgcct acccgtgcag ctcggagcgc gcagcccgtc ttggaataag gagtgagtac
       61 aatggcgtgt tggtaaaaac aaaaaagaaa aaaaaaagaa agaaagaaaa agaaaagagg
      121 aaagaaagaa agaaagaaag aaggaaagaa ggaaagaaag agaaaagaaa agaaaacaga
      181 gagagagaga gaaagaaaag aaaaaagcac caagtctgtg ttaaaacaaa caaacaaaca
      241 aacattttga atgctgcatg cctcatgctt gccagcgcct cgcgggagag acccggctat
      301 actgcaggag tggcggcccc tgacttgctg gatcccaagt ctgctgctca gaactccaaa
      361 ccgaggctct cattctcctc aaaacccacc gtgcttgctt cccgggtgga gagtgactcg
      421 gccattaatg ttatgaaatg gaagacagtc tccacgattt tcctggtggt cgtcctctac
      481 ctgatcatcg gagccacggt gttcaaggca ttggagcagc ctcaggagat ttcccagagg
      541 accaccattg tgatccagaa gcagaccttc atagcccagc atgcctgcgt caactccacc
      601 gagctggacg aactcatcca gcaaatagtg gcagcaataa acgcagggat tatcccctta
      661 ggaaacagct ccaatcaagt tagtcactgg gacctcggaa gctctttctt ctttgctggt
      721 actgttatca caaccatagg atttggaaac atctccccac gaactgaagg tggaaaaata
      781 ttctgcatca tctatgcctt gctgggaatt cccctctttg gctttctact ggctggggtt
      841 ggtgatcagc taggaactat atttggaaaa ggaattgcca aagtggaaga cacatttatt
      901 aagtggaatg ttagtcagac gaagattcgt atcatctcca ccatcatctt catcctgttt
      961 ggctgtgtcc tctttgtggc tctccctgcg gtcatattca agcacataga aggctggagc
     1021 gccctggacg ctatctattt tgtggttatc actctgacga ccattggatt tggagactac
     1081 gtggcaggtg gatcagacat tgaatatctg gacttctaca agcctgtggt gtggttctgg
     1141 atcctcgttg ggctggccta ctttgcagct gttctgagca tgattgggga ctggctacgg
     1201 gtgatctcta agaagacgaa ggaagaggtg ggagagttca gagcgcatgc cgctgagtgg
     1261 acagccaatg tcacggccga gttcaaggaa acgaggaggc ggctgagcgt ggagatctac
     1321 gacaagttcc agcgtgccac atccgtgaag cggaagctct ccgcagagct ggcgggcaac
     1381 cacaaccagg aactgactcc gtgtaggagg accctgtctg tgaaccacct gaccagcgag
     1441 agggaagtcc tgcctccctt gctgaaggct gagagcatct atctgaacgg tctgacacca
     1501 cactgtgctg gtgaggacat agctgtcatt gagaacatga agtagccctc tcttggaaga
     1561 gtctgaggtg gagccatagg gaagggcttc tctaggctct ttgtgactgt tgccggtagc
     1621 atttaaacat tgtgcatggt gacctcaaag ggaaagcaaa tagaaaacac ccatctggtc
     1681 accttacatc cagggagggt gttgtcccga ggcggcactc tgaggatgcc gtgtgctgtc
     1741 cgctgagtgc tgagtgatgg acaggcagtg tctgatgcct tttgtgccca gactgtttcc
     1801 cctccccctc tctcctaacg tgccataagg cctatgaatg aatctgaatg ctttgctggt
     1861 catgtagatt ggagggatca gcccttggtt tttcatggtt cacctaactg agcctggata
     1921 ctgaccactt agggatgaca acatttcttt ttgtaaatgg cgagaaattc ttacgcagcc
     1981 ttttacctaa gaaattttct gccagtgcct tatcttatga agaaacaaga ccctgtctgg
     2041 tgggcttgtg gttcctccct tccccgccct caccctgggc tcaccctttg ctgactctca
     2101 cctatcccct cccctccgca taccttattg tgctggaagc aatgtgtggc ttgatggaag
     2161 aaacagatgc caactgcagg cacagaagca acatggaagc tctggcgtca cgggcactgc
     2221 agagaaggga ggtcagagaa ggcccctctg agtatttatt tgaccggggt accaatggta
     2281 cagctatata tatgtacaga gtaataattc ccaggccggt aaccttggct gcttccacac
     2341 ggcccccttt tttccctggc agtatttgaa gtttatcatt tattaataac tagtcatttt
     2401 taaaggcaga agaagacatg agcacataca tctgtaatct acgtgatgtg ataagaaaac
     2461 tgttcagact ggtattgcaa ggtgatctca gacagtcgat atggattcat tctgatgaga
     2521 agaaaacagc gagaccacgt gtcgtgcgca tgagctcata cagggctcac tgtcagcttg
     2581 gaccagcccg tgaggtacag caggcttaca cactttcttt tcttaagtgg ttcttgccaa
     2641 actggaggga ggggcgatgc ccttcagaag ggggcacagc cccagccagc ccagggtccc
     2701 tctgtggtca tgactgggtg tgagcacaga tgctggcctt gggatcactg tgagttttgc
     2761 acatggagag atacagactg ctggcatagg tcgtctctaa cagtagagaa aacgccgatt
     2821 agcacaatct aaatcccccg agttactttt tgtttaggat aagagaaggc tggtaattca
     2881 cttaatttaa atttatatcc tataattctt tttggatgtt tcaagattca gaaaaagtcc
     2941 agtccctgca tctagcaaac cgccgccctt tcctctgtgc ccgtacttac atctactgaa
     3001 cactgtatat gtaattttta aatttttaaa gcgcagaagg aaaatgattc ttctacatgt
     3061 aatcgcaaaa ctgatttctc ccttctgggg gaggcttggg cttacgtgat catgtggcat
     3121 tcagagtaaa gtcttaagac ataaacccag gatgtttaaa aacacaaaaa gattcctatt
     3181 ttccaaaatt tgaatttaag ctataaatgt aaatatatcc agttcgcttg cagagagtac
     3241 ttttataaac ctatgaagat tctaaccaaa attttaaaat gtcgggttcc tttacaaaga
     3301 aagctccacc catctcctga atagccgttc tggaagctga catggtggga tgtaccacgt
     3361 ataaactgtg aactggagga caaataaagt ttgtaattaa aatca
//
