LOCUS       NM_146037               3061 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus potassium channel, subfamily K, member 13 (Kcnk13),
            transcript variant 3, mRNA.
ACCESSION   NM_146037 XM_358425
VERSION     NM_146037.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Rifat,A., Ossola,B., Burli,R.W., Dawson,L.A., Brice,N.L.,
            Rowland,A., Lizio,M., Xu,X., Page,K., Fidzinski,P., Onken,J.,
            Holtkamp,M., Heppner,F.L., Geiger,J.R.P. and Madry,C.
  TITLE     Differential contribution of THIK-1 K+ channels and P2X7 receptors
            to ATP-mediated neuroinflammation by human microglia
  JOURNAL   J Neuroinflammation 21 (1), 58 (2024)
   PUBMED   38409076
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3061)
  AUTHORS   Ossola,B., Rifat,A., Rowland,A., Hunter,H., Drinkall,S., Bender,C.,
            Hamlischer,M., Teall,M., Burley,R., Barker,D.F., Cadwalladr,D.,
            Dickson,L., Lawrence,J.M.K., Harvey,J.R.M., Lizio,M., Xu,X.,
            Kavanagh,E., Cheung,T., Sheardown,S., Lawrence,C.B., Harte,M.,
            Brough,D., Madry,C., Matthews,K., Doyle,K., Page,K., Powell,J.,
            Brice,N.L., Burli,R.W., Carlton,M.B. and Dawson,L.A.
  TITLE     Characterisation of C101248: A novel selective THIK-1 channel
            inhibitor for the modulation of microglial NLRP3-inflammasome
  JOURNAL   Neuropharmacology 224, 109330 (2023)
   PUBMED   36375694
REFERENCE   4  (bases 1 to 3061)
  AUTHORS   Drinkall,S., Lawrence,C.B., Ossola,B., Russell,S., Bender,C.,
            Brice,N.B., Dawson,L.A., Harte,M. and Brough,D.
  TITLE     The two pore potassium channel THIK-1 regulates NLRP3 inflammasome
            activation
  JOURNAL   Glia 70 (7), 1301-1316 (2022)
   PUBMED   35353387
  REMARK    GeneRIF: The two pore potassium channel THIK-1 regulates NLRP3
            inflammasome activation.
REFERENCE   5  (bases 1 to 3061)
  AUTHORS   Izquierdo,P., Shiina,H., Hirunpattarasilp,C., Gillis,G. and
            Attwell,D.
  TITLE     Synapse development is regulated by microglial THIK-1 K+ channels
  JOURNAL   Proc Natl Acad Sci U S A 118 (42) (2021)
   PUBMED   34642249
REFERENCE   6  (bases 1 to 3061)
  AUTHORS   Sakamaki,K., Ishii,T.M., Sakata,T., Takemoto,K., Takagi,C.,
            Takeuchi,A., Morishita,R., Takahashi,H., Nozawa,A., Shinoda,H.,
            Chiba,K., Sugimoto,H., Saito,A., Tamate,S., Satou,Y., Jung,S.K.,
            Matsuoka,S., Koyamada,K., Sawasaki,T., Nagai,T. and Ueno,N.
  TITLE     Dysregulation of a potassium channel, THIK-1, targeted by caspase-8
            accelerates cell shrinkage
  JOURNAL   Biochim Biophys Acta 1863 (11), 2766-2783 (2016)
   PUBMED   27566292
  REMARK    GeneRIF: Dysregulation of a potassium channel, THIK-1, targeted by
            caspase-8, accelerates cell shrinkage.
REFERENCE   7  (bases 1 to 3061)
  AUTHORS   Miller,J.A., Nathanson,J., Franjic,D., Shim,S., Dalley,R.A.,
            Shapouri,S., Smith,K.A., Sunkin,S.M., Bernard,A., Bennett,J.L.,
            Lee,C.K., Hawrylycz,M.J., Jones,A.R., Amaral,D.G., Sestan,N.,
            Gage,F.H. and Lein,E.S.
  TITLE     Conserved molecular signatures of neurogenesis in the hippocampal
            subgranular zone of rodents and primates
  JOURNAL   Development 140 (22), 4633-4644 (2013)
   PUBMED   24154525
REFERENCE   8  (bases 1 to 3061)
  AUTHORS   Schilling,K. and Oberdick,J.
  TITLE     The treasury of the commons: making use of public gene expression
            resources to better characterize the molecular diversity of
            inhibitory interneurons in the cerebellar cortex
  JOURNAL   Cerebellum 8 (4), 477-489 (2009)
   PUBMED   19554387
REFERENCE   9  (bases 1 to 3061)
  AUTHORS   Theilig,F., Goranova,I., Hirsch,J.R., Wieske,M., Unsal,S.,
            Bachmann,S., Veh,R.W. and Derst,C.
  TITLE     Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1)
            K channels in the mammalian kidney
  JOURNAL   Cell Physiol Biochem 21 (1-3), 63-74 (2008)
   PUBMED   18209473
  REMARK    GeneRIF: THIK-1 and THIK-2 are abundantly expressed in the proximal
            and distal nephron of the mammalian kidney.
REFERENCE   10 (bases 1 to 3061)
  AUTHORS   Harrell,M.D., Harbi,S., Hoffman,J.F., Zavadil,J. and Coetzee,W.A.
  TITLE     Large-scale analysis of ion channel gene expression in the mouse
            heart during perinatal development
  JOURNAL   Physiol Genomics 28 (3), 273-283 (2007)
   PUBMED   16985003
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159243.2.
            
            On Aug 26, 2009 this sequence version replaced NM_146037.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158645.1, SRR7345562.2953040.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-303               AC159243.2         7393-7695
            304-1380            AC159243.2         8098-9174
            1381-3061           AC159243.2         103896-105576
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 50.43 cM"
     gene            1..3061
                     /gene="Kcnk13"
                     /gene_synonym="F730021E22Rik; Gm1570; Gm1685"
                     /note="potassium channel, subfamily K, member 13"
                     /db_xref="GeneID:217826"
                     /db_xref="MGI:MGI:2384976"
     exon            1..303
                     /gene="Kcnk13"
                     /gene_synonym="F730021E22Rik; Gm1570; Gm1685"
                     /inference="alignment:Splign:2.1.0"
     exon            304..1380
                     /gene="Kcnk13"
                     /gene_synonym="F730021E22Rik; Gm1570; Gm1685"
                     /inference="alignment:Splign:2.1.0"
     CDS             1047..2264
                     /gene="Kcnk13"
                     /gene_synonym="F730021E22Rik; Gm1570; Gm1685"
                     /note="THIK-1; tandem pore domain halothane-inhibited
                     potassium channel 1"
                     /codon_start=1
                     /product="potassium channel subfamily K member 13"
                     /protein_id="NP_666149.1"
                     /db_xref="CCDS:CCDS26106.1"
                     /db_xref="GeneID:217826"
                     /db_xref="MGI:MGI:2384976"
                     /translation="MAGRGCGCSPGHLNEDNARFLLLAGLILLYLLGGAAVFSALELA
                     QELQAKQRWEERLANFSRGHNLSREELRGFLRHYEEATRAGIRMDSVRPRWDFTGAFY
                     FVGTVVSTIGFGMTTPATTGGKIFLIFYGLIGCASTILFFNLFLERLITVIACVMRSC
                     HQQQLRRRGAVTQDNMKAPEKGEADSLTGWKPSVYYVMLILCLASVAISCGASALYTT
                     MEGWSYFDSVYFCFVAFSTIGFGDLVSSQNAQYESQGLYRFFNFFLILMGVCCIYSLF
                     NVISILIKQTVNWILRKLDSGCFPPCQRGLLRSRRNVVMPGNIRNRCNISIETDGVME
                     SDTDGRRLSGEMISMKDTNKVSLAILQKQLSEMANGGPHQNSASSRDDEFSGGVGAFA
                     VMNNRLAETSGDR"
     exon            1381..3061
                     /gene="Kcnk13"
                     /gene_synonym="F730021E22Rik; Gm1570; Gm1685"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3035..3040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnk13"
                     /gene_synonym="F730021E22Rik; Gm1570; Gm1685"
                     /note="hexamer: AATAAA"
     polyA_site      3055
                     /gene="Kcnk13"
                     /gene_synonym="F730021E22Rik; Gm1570; Gm1685"
                     /note="major polyA site"
ORIGIN      
        1 gacctatctg ttaagcaacc actccaggga tctgcactcc ctggtagaag catcgctgtt
       61 tagaggtcca ggcgagcgcc agcggaggag acctgagcag gagactggcc ctgaggccaa
      121 ggggcgggta ccgggtggag tcgcctcggt tggcagctgc agagctccac ctgttgcagt
      181 ttttgagcac tccacagaga aggcagctct gggcacggac ttctgctctc agccccactg
      241 ggcagggcaa ggccacttga gaagggaagc caggaaccat cctgaacctg ttccacagag
      301 cagcggtccg ccctcgtgct gccgtggagc ctgtgtgccc agcagagatc cgagccggct
      361 gatctgcctc ttcgcactgt ccctgcgtcc gtgcgtctgc cccaaacccg ttccgacctc
      421 tgagccctgg ttattctggt ggatagaggc tctcggcaga acattgtcac taatacgcaa
      481 cacagatcca cctggagagg caccgaagac agagaacagc ccgtatccca gccatcacag
      541 cttggaaagc aatgctccga ggaacccgga ccttgatgtg aagcttaccc gccgattctc
      601 tctggaccca cgaccttttt tttcttcaag aggagttcaa gttctaggga gtttgccttg
      661 aaggcacgcc acgccccctc cccaatctga gccgaattag gagctcgggt gaacccgggt
      721 cgcccgcacc ctcgcaccaa gtcgaagcag attctgtgtc ccgaccctct ggtcttcagc
      781 cttaaggaga actccagaga ggacagggag gcggcgacga caaagcggat tcgaaaccac
      841 gagtccaggc ggacgtgggg agctggctac acaggaacca ccgaggaggc ggcggcggcc
      901 gggagatcca cgccgccttg gggaacgcgc ggcgggcatg ggcgagcccg cacgcagagc
      961 aggctaaggt cggcagagca catcctcccc ggggcgtggg cacaagtccg cgggcagcaa
     1021 ccctcgcgga gctgtcctcc agtgccatgg ctggccgcgg ttgcggctgc agccccggcc
     1081 acctgaacga ggacaacgcg cgcttcctgc tgctcgctgg gctcatcctg ctctacctgc
     1141 tgggcggcgc cgcagtcttc tccgcgctgg agctagcgca ggagttgcag gccaagcagc
     1201 gctgggaaga gcgcctggcc aacttcagcc ggggccacaa cctgagccgt gaagagctgc
     1261 gaggtttcct ccgccactac gaggaagcca ccagggcggg catccgcatg gacagcgtgc
     1321 gccctcgctg ggacttcacg ggcgccttct acttcgtggg tacagtggtt tctaccatag
     1381 ggtttgggat gacaacacca gccacaacgg gagggaagat ttttctgatc ttttatggtc
     1441 tcattggatg tgcaagtacc atcctcttct tcaacctttt cctggagcgg ctgatcactg
     1501 tcatcgcctg tgtcatgaga tcctgtcacc agcagcagct gcgcagacgt ggggcggtga
     1561 cccaggacaa catgaaggct cctgaaaagg gggaggcaga cagcctgact ggctggaagc
     1621 cctctgtgta ctacgtcatg ctgatcctat gcttggcatc agtggccatc tcctgcggag
     1681 cctctgctct gtacaccacc atggagggct ggagctactt tgactcggtc tacttctgtt
     1741 ttgtggcttt cagcaccatt ggcttcgggg acctggtgag cagccagaat gctcagtatg
     1801 agagccaagg actctaccgc ttcttcaatt tcttcctcat cctcatgggt gtctgctgca
     1861 tctactcttt gtttaacgtc atctccatcc tgatcaaaca gactgtgaac tggatcctga
     1921 ggaaactgga tagcgggtgc ttcccaccat gccaaagagg actcctgcgg tccaggagga
     1981 atgtggtgat gccgggtaac atccggaaca ggtgcaacat ctccatagag acagacgggg
     2041 tgatggaaag tgacactgat ggacgacgtc tctcggggga gatgatctcc atgaaggaca
     2101 ccaacaaggt ctccctggcc atcctgcaga agcagttgtc cgagatggcc aatgggggac
     2161 cccaccagaa cagtgcatcc tcccgggatg atgagttctc agggggagtg ggagcctttg
     2221 cagtaatgaa taacaggttg gcagagacca gtggagatag gtaggaacca gaagtagctg
     2281 ctatatgaag aaaaccaggg catgtgggga ataagacact gttacaccac tggtaagctc
     2341 agctgtgcct ctggctgttg caattaatat ctccagtcca ggttttgaaa tctgaccttg
     2401 gcctcaggca agagtagcct ctcacagttg agggctggag cctctttccc tggctcttac
     2461 tttacttttg aattccaagt ttgacatttg gaactgctaa gggccttgcc ttcttccaga
     2521 gttgtaatgg tctgagggag actggtccag ttttggtttc tccaccatga tccattctta
     2581 ggaagggaga gcccccaaaa agcctgcttc tgtcctggaa cgtgagtggc tgaacttcac
     2641 ccagcccctc aagaaaaggc aacaaacatt ccagagtgtc tctgggcctt ctgtttgctg
     2701 gacttccttc caaacatatc tattgttggt tccattgatg gggacactat ctcatcttct
     2761 cttatacctg cttctctgtt tgggagtctt ggtttctttt tgttttgttt cctgtttgac
     2821 taatatatct tttggatctt atgtgataag aaaactaaat ccaccttcaa acctttaagc
     2881 ttggcagtga gtagaggaag agggaaactc catcttgtaa ctaaagccca cagcttacta
     2941 tgtcctctct gaatggttac acggatcgtg tcattttacc agggcttgga gcctggtgca
     3001 gaggcaactg actatttatt agagttgaac atttaataaa ttctcatttt gttaactacg
     3061 a
//
