LOCUS       NM_001252209            3685 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus potassium inwardly-rectifying channel, subfamily J,
            member 16 (Kcnj16), transcript variant 3, mRNA.
ACCESSION   NM_001252209
VERSION     NM_001252209.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Zhou,H.M., Xu,H.J., Sun,R.H., Zhang,M., Li,X.T., Zhao,Y.X.,
            Yang,K., Wei,R., Liu,Q., Li,S., Xue,Z., Hao,L.Y., Yang,L.,
            Wang,Q.H., Wang,H.J., Gao,F., Cao,J.L. and Pan,Z.
  TITLE     DNA N6-methyladenine methylase N6AMT1 controls neuropathic pain
            through epigenetically modifying Kcnj16 in dorsal horn neurons
  JOURNAL   Pain 165 (1), 75-91 (2024)
   PUBMED   37624905
  REMARK    GeneRIF: DNA N6-methyladenine methylase N6AMT1 controls neuropathic
            pain through epigenetically modifying Kcnj16 in dorsal horn
            neurons.
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Xie,Z., Ma,X.H., Bai,Q.F., Tang,J., Sun,J.H., Jiang,F., Guo,W.,
            Wang,C.M., Yang,R., Wen,Y.C., Wang,F.Y., Chen,Y.X., Zhang,H.,
            He,D.Z., Kelley,M.W., Yang,S. and Zhang,W.J.
  TITLE     ZBTB20 is essential for cochlear maturation and hearing in mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (24), e2220867120 (2023)
   PUBMED   37279265
REFERENCE   3  (bases 1 to 3685)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3685)
  AUTHORS   Poli,G., Hasan,S., Belia,S., Cenciarini,M., Tucker,S.J.,
            Imbrici,P., Shehab,S., Pessia,M., Brancorsini,S. and D'Adamo,M.C.
  TITLE     Kcnj16 (Kir5.1) Gene Ablation Causes Subfertility and Increases the
            Prevalence of Morphologically Abnormal Spermatozoa
  JOURNAL   Int J Mol Sci 22 (11), 5972 (2021)
   PUBMED   34205849
  REMARK    GeneRIF: Kcnj16 (Kir5.1) Gene Ablation Causes Subfertility and
            Increases the Prevalence of Morphologically Abnormal Spermatozoa.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3685)
  AUTHORS   Lv,J., Fu,X., Li,Y., Hong,G., Li,P., Lin,J., Xun,Y., Fang,L.,
            Weng,W., Yue,R., Li,G.L., Guan,B., Li,H., Huang,Y. and Chai,R.
  TITLE     Deletion of Kcnj16 in Mice Does Not Alter Auditory Function
  JOURNAL   Front Cell Dev Biol 9, 630361 (2021)
   PUBMED   33693002
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3685)
  AUTHORS   Harrell,M.D., Harbi,S., Hoffman,J.F., Zavadil,J. and Coetzee,W.A.
  TITLE     Large-scale analysis of ion channel gene expression in the mouse
            heart during perinatal development
  JOURNAL   Physiol Genomics 28 (3), 273-283 (2007)
   PUBMED   16985003
REFERENCE   7  (bases 1 to 3685)
  AUTHORS   Hibino,H., Fujita,A., Iwai,K., Yamada,M. and Kurachi,Y.
  TITLE     Differential assembly of inwardly rectifying K+ channel subunits,
            Kir4.1 and Kir5.1, in brain astrocytes
  JOURNAL   J Biol Chem 279 (42), 44065-44073 (2004)
   PUBMED   15310750
  REMARK    GeneRIF: Kir4.1/5.1 and Kir4.1 expression appeared to occur only in
            astrocytes, specifically in the membrane domains facing the pia
            mater and blood vessels or in the processes surrounding synapses.
REFERENCE   8  (bases 1 to 3685)
  AUTHORS   Sierra,D.A., Gilbert,D.J., Householder,D., Grishin,N.V., Yu,K.,
            Ukidwe,P., Barker,S.A., He,W., Wensel,T.G., Otero,G., Brown,G.,
            Copeland,N.G., Jenkins,N.A. and Wilkie,T.M.
  TITLE     Evolution of the regulators of G-protein signaling multigene family
            in mouse and human
  JOURNAL   Genomics 79 (2), 177-185 (2002)
   PUBMED   11829488
REFERENCE   9  (bases 1 to 3685)
  AUTHORS   Liu,Y., McKenna,E., Figueroa,D.J., Blevins,R., Austin,C.P.,
            Bennett,P.B. and Swanson,R.
  TITLE     The human inward rectifier K(+) channel subunit kir5.1 (KCNJ16)
            maps to chromosome 17q25 and is expressed in kidney and pancreas
  JOURNAL   Cytogenet Cell Genet 90 (1-2), 60-63 (2000)
   PUBMED   11060447
REFERENCE   10 (bases 1 to 3685)
  AUTHORS   Mouri,T., Kittaka,N., Horio,Y., Copeland,N.G., Gilbert,D.J.,
            Jenkins,N.A. and Kurachi,Y.
  TITLE     Assignment of mouse inwardly rectifying potassium channel Kcnj16 to
            the distal region of mouse chromosome 11
  JOURNAL   Genomics 54 (1), 181-182 (1998)
   PUBMED   9806850
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL592422.11, AK045447.1 and AW122947.1.
            
            On Nov 9, 2012 this sequence version replaced NM_001252209.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. Variants 1-5 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140249.1, AK045447.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AL592422.11        135437-135482
            47-2449             AK045447.1         3-2405
            2450-3281           AL592422.11        164697-165528
            3282-3685           AW122947.1         1-404               c
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 75.01 cM"
     gene            1..3685
                     /gene="Kcnj16"
                     /gene_synonym="6430410F18Rik; Kir5.1"
                     /note="potassium inwardly-rectifying channel, subfamily J,
                     member 16"
                     /db_xref="GeneID:16517"
                     /db_xref="MGI:MGI:1314842"
     exon            1..111
                     /gene="Kcnj16"
                     /gene_synonym="6430410F18Rik; Kir5.1"
                     /inference="alignment:Splign:2.1.0"
     exon            112..3671
                     /gene="Kcnj16"
                     /gene_synonym="6430410F18Rik; Kir5.1"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..1476
                     /gene="Kcnj16"
                     /gene_synonym="6430410F18Rik; Kir5.1"
                     /note="inward rectifier K(+) channel Kir5.1; potassium
                     channel, inwardly rectifying subfamily J member 16"
                     /codon_start=1
                     /product="inward rectifier potassium channel 16"
                     /protein_id="NP_001239138.1"
                     /db_xref="CCDS:CCDS25593.1"
                     /db_xref="GeneID:16517"
                     /db_xref="MGI:MGI:1314842"
                     /translation="MSYYGSSYRIVNVDSKYPGYPPEHAIAEKRRARRRLLHKDGSCN
                     VYFKHIFGEWGSYMVDIFTTLVDTKWRHMFVIFSLSYILSWLIFGSIFWLIAFHHGDL
                     LSDPDITPCVDNVHSFTAAFLFSLETQTTIGYGYRCVTEECSVAVLTVILQSILSCII
                     NTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRA
                     QLLRYSEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNF
                     EILVTFIYTGDSTGTSHQSRSSYIPREILWGHRFHDVLEVKRKYYKVNCLQFEGSVEV
                     YAPFCSAKQLDWKDQQLNNLEKTSPARGSCNSDTNTRRRSFSAVAVVSSCENPEETVL
                     SPQDECKEMPYQKALLTLNRISMESQM"
     regulatory      3647..3652
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnj16"
                     /gene_synonym="6430410F18Rik; Kir5.1"
                     /note="hexamer: AATAAA"
     polyA_site      3671
                     /gene="Kcnj16"
                     /gene_synonym="6430410F18Rik; Kir5.1"
                     /note="major polyA site"
ORIGIN      
        1 actgacagcc tcagaagggg aacaagaaga gcgcagagaa gagatcatga gttctgtctg
       61 ggcagtcgtg ttgaagatca aaaggcccca gggcttggat ctgtaactga ggctttaaca
      121 gaaaatcttt aaaaaaaaaa aagtagaagt tagaatacac ctggaacttt taactgacat
      181 cggactcctc tgaatgccct aaaggaccaa gaaagaatga gctattacgg aagtagctac
      241 aggatcgtca atgtggactc caaatatcca ggctatcctc cagagcatgc catcgctgag
      301 aagagaagag caagaaggcg cttgctccac aaagatggca gctgtaatgt gtactttaaa
      361 cacatttttg gagaatgggg gagctacatg gttgatattt ttaccactct tgtggatacc
      421 aagtggcgcc atatgttcgt aatattttct ctgtcttaca ttctctcctg gttgatattt
      481 ggctccatat tttggctcat agcctttcat cacggagacc tattaagcga tccagatatc
      541 accccttgtg ttgacaacgt gcattcattt acggctgcat ttttattctc cctggagacc
      601 cagaccacca ttggatacgg ttaccgctgt gtcaccgaag agtgctctgt ggctgtactg
      661 acagtgatcc ttcagtccat cctcagctgc atcataaaca ccttcatcat tggagcagcc
      721 ttggcaaaga tggcaactgc ccggaagaga gcccagacca tacgcttcag ctattttgcc
      781 ctcattggta tgagagacgg gaagctttgc ctcatgtggc gcataggtga cttccgacca
      841 aaccatgtgg tagagggcac ggtgagagcc caacttctgc gctattcaga agacagtgaa
      901 gggaggatga cgatggcgtt taaagacctc aaactcgtca atgaccagat aatcctggta
      961 actccagtga ctattgtcca tgaaattgac catgagagcc ctctgtatgc ccttgaccgc
     1021 aaggcagtgg ccaaagataa tttcgagatt ctggtgacat ttatttatac tggtgattcc
     1081 actgggacat cccaccagtc cagaagttcc tacatcccca gagaaattct ctggggccac
     1141 aggtttcatg atgtattgga agtgaagaga aagtactaca aggtgaactg cttgcagttt
     1201 gaaggaagcg tggaagtcta cgcccccttt tgcagtgcca aacaactgga ctggaaggac
     1261 caacaactca acaacttgga gaaaacgtcc cctgcccgag gatcctgcaa ttctgacacc
     1321 aacaccagga ggcggtcctt cagcgcagtt gccgtggtga gcagctgtga gaacccagag
     1381 gagaccgtcc tgtccccaca agatgaatgt aaggagatgc cctatcagaa agccctcctg
     1441 actttaaata ggatctccat ggaatcccag atgtagccct cctctccgac ccttatctaa
     1501 gggccagaag ctgtaagcat gatttttaac aaagtgttag ttctgtgatt tgggacagga
     1561 atagaaagca gagcaggtta aacttggtga attattatct aaaaaatcca caattttaca
     1621 atattaaaat ttggctttgg cagtagagtt tgtattaggg aagtgattat gaagcttaat
     1681 tgattacaat taatctcatt attgcatata cctgtgtctc ctcttgaagg tgaaatattt
     1741 aggagtgggg gaaaaagagg ctgttaaacc agataaatat agagaagcag ataagctaaa
     1801 tatttgcata taaaaataaa ttaattatgg aatgagaact tataaccgta ttgtatcacc
     1861 taagcacagc aggtgaaata tgcctccgct cagaaacact gagatgagaa agtaaaacca
     1921 agtgaagttt gagtttacca catttgcctc tcatagtatc ttgcagacag aggtgtggct
     1981 ttaggaaagt gaaaatgcta tcaagtcaga gatgcttgcc taaagtgtta ttagtgaatg
     2041 ctggaagcca gaaccaacca tgtgacagaa gaaaagggac atcattacgt aaattagtat
     2101 ttctctctta aataccttgg ttcaaccgag tcactgggga gcagaaggga ggcggtggcc
     2161 tttctgtgtg ctccctggag aaggttcctg tgctcataat cccactgtgc tcaagcattt
     2221 gtgaagaggc aaggtgggat gcaggagtgg gcagcagctg tgtgcatcct cagacctttg
     2281 gagaagagat gctagatgct cttgattcct gatcctgaga ggctacagtg tgggttgagg
     2341 agaattcaaa gagatagaac ctttcctgaa ggcagaggat cacagtatgt atggtgacct
     2401 gagttgcagg tctgaatact tttgtcaaca atgatgttga tttgattgta aataagagga
     2461 ataaactgaa tattatgggg ctgcttaaat tttaggacaa gagatgcatg tttattctct
     2521 gtcttcacat taaaggatta agttaattgc tgattccttt ttagcacgaa cccagccaca
     2581 gagaccgtaa gctttataaa ctgaggaatt attagccatg gcttggcatc actggagcaa
     2641 ttttttccca ctgttgctga gctaggaagg aggacattat atggctggta ttgaatttaa
     2701 gcacaaacac attttgattc ttttggtgcc ttctttctct ctcttcctct tattctctct
     2761 ctctctctct ctctctctct cacacacaca cacacacaca cacacacaca cacacacaca
     2821 cacacacaca cttaaattta atcccatagg cacactgaac tgaatcacac aattcaagac
     2881 ttccaagcta agtcaaataa atagatattc taaggggtct ctttttcttc tttttttctg
     2941 ttattttttt tctcccactc cctttctttc tttacctctc ccttgttacc caggatcaat
     3001 acagaataca gtgtaacttt tctaatgcac atgtgtagat aaatctttcg ttcctgctgt
     3061 catataaata agagatctat ctgttcatgg ggcatgttaa aaactgtcct ggacttctta
     3121 atatctgaaa cctaaatcat gtttaacctg caatacttgg gaatggagaa tccattagtt
     3181 aaaccataga gccatgtatt tatacaccca cacatctggc atgcacacac acacacacac
     3241 acacacacac acacacacac acacacacac gcacacacac acggtggggt gaggtgggtg
     3301 agacagacac acagagaaaa ttcgaatttt ttagcaggaa ttacttgtct aaattagaat
     3361 attacccttt ccaaggctgg aaaacatcgc tctaaccatg ctttctccac cagtttaaat
     3421 ttgttttcct tcaaggcagg tacaatccag tgcaccaggc ttcctttgct ctgtcctcct
     3481 atgacaagat cagaatgggg tggcggtggt cagggatcac aggtctctga ttaaacagat
     3541 tctggtgcac tgtgtgttca tacacagagt gaaacgtgtg gtcccatgca tctctcatct
     3601 tctgtgcaga tttataacat gctttgacac gcctgatggc ttacttaata aagatcttag
     3661 actggtgcaa aaaaaaaaaa aaaaa
//
