LOCUS       NM_001267593            4427 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus potassium inwardly-rectifying channel, subfamily J,
            member 12 (Kcnj12), transcript variant 2, mRNA.
ACCESSION   NM_001267593
VERSION     NM_001267593.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Zhang,X., Liao,C., Sun,K., Liu,L. and Xu,D.
  TITLE     A Soluble Epoxide Hydrolase Inhibitor Upregulated KCNJ12 and KCNIP2
            by Downregulating MicroRNA-29 in a Mouse Model of Myocardial
            Infarction
  JOURNAL   Heart Surg Forum 23 (5), E579-E585 (2020)
   PUBMED   32990585
  REMARK    GeneRIF: A Soluble Epoxide Hydrolase Inhibitor Upregulated KCNJ12
            and KCNIP2 by Downregulating MicroRNA-29 in a Mouse Model of
            Myocardial Infarction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4427)
  AUTHORS   Lalancette-Hebert,M., Sharma,A., Lyashchenko,A.K. and Shneider,N.A.
  TITLE     Gamma motor neurons survive and exacerbate alpha motor neuron
            degeneration in ALS
  JOURNAL   Proc Natl Acad Sci U S A 113 (51), E8316-E8325 (2016)
   PUBMED   27930290
REFERENCE   4  (bases 1 to 4427)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   5  (bases 1 to 4427)
  AUTHORS   Gineste,C., De Winter,J.M., Kohl,C., Witt,C.C., Giannesini,B.,
            Brohm,K., Le Fur,Y., Gretz,N., Vilmen,C., Pecchi,E., Jubeau,M.,
            Cozzone,P.J., Stienen,G.J., Granzier,H., Labeit,S.,
            Ottenheijm,C.A., Bendahan,D. and Gondin,J.
  TITLE     In vivo and in vitro investigations of heterozygous nebulin
            knock-out mice disclose a mild skeletal muscle phenotype
  JOURNAL   Neuromuscul Disord 23 (4), 357-369 (2013)
   PUBMED   23375831
REFERENCE   6  (bases 1 to 4427)
  AUTHORS   Probst,F.J., Chen,K.S., Zhao,Q., Wang,A., Friedman,T.B.,
            Lupski,J.R. and Camper,S.A.
  TITLE     A physical map of the mouse shaker-2 region contains many of the
            genes commonly deleted in Smith-Magenis syndrome (del17p11.2p11.2)
  JOURNAL   Genomics 55 (3), 348-352 (1999)
   PUBMED   10049592
REFERENCE   7  (bases 1 to 4427)
  AUTHORS   Liang,Y., Wang,A., Probst,F.J., Arhya,I.N., Barber,T.D., Chen,K.S.,
            Deshmukh,D., Dolan,D.F., Hinnant,J.T., Carter,L.E., Jain,P.K.,
            Lalwani,A.K., Li,X.C., Lupski,J.R., Moeljopawiro,S., Morell,R.,
            Negrini,C., Wilcox,E.R., Winata,S., Camper,S.A. and Friedman,T.B.
  TITLE     Genetic mapping refines DFNB3 to 17p11.2, suggests multiple alleles
            of DFNB3, and supports homology to the mouse model shaker-2
  JOURNAL   Am J Hum Genet 62 (4), 904-915 (1998)
   PUBMED   9529344
REFERENCE   8  (bases 1 to 4427)
  AUTHORS   Takumi,T., Tsuji,L., Kondo,C., Takahashi,N., Morishige,K.,
            Copeland,N.G., Gilbert,D.J., Jenkins,N.A. and Kurachi,Y.
  TITLE     Assignment of the murine inward rectifier potassium channel Irk2
            (Kir2.2) gene to the central region of mouse chromosome 11
  JOURNAL   Genomics 37 (2), 270-272 (1996)
   PUBMED   8921409
REFERENCE   9  (bases 1 to 4427)
  AUTHORS   Doupnik,C.A., Davidson,N. and Lester,H.A.
  TITLE     The inward rectifier potassium channel family
  JOURNAL   Curr Opin Neurobiol 5 (3), 268-277 (1995)
   PUBMED   7580148
  REMARK    Review article
REFERENCE   10 (bases 1 to 4427)
  AUTHORS   Takahashi,N., Morishige,K., Jahangir,A., Yamada,M., Findlay,I.,
            Koyama,H. and Kurachi,Y.
  TITLE     Molecular cloning and functional expression of cDNA encoding a
            second class of inward rectifier potassium channels in the mouse
            brain
  JOURNAL   J Biol Chem 269 (37), 23274-23279 (1994)
   PUBMED   8083233
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK032271.1 and AL646093.9.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region and initiates translation at a
            downstream, in-frame start codon, compared to variant 1. The
            encoded isoform (2) has a shorter N-terminus, compared to isoform
            1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032271.1, SRR7974084.22577.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AK032271.1         2-122
            122-122             AL646093.9         35203-35203
            123-327             AK032271.1         124-328
            328-2011            AK032271.1         330-2013
            2012-4427           AL646093.9         83370-85785
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 37.96 cM"
     gene            1..4427
                     /gene="Kcnj12"
                     /gene_synonym="IRK-2; IRK2; Kir2.2; MB-IRK2"
                     /note="potassium inwardly-rectifying channel, subfamily J,
                     member 12"
                     /db_xref="GeneID:16515"
                     /db_xref="MGI:MGI:108495"
     exon            1..287
                     /gene="Kcnj12"
                     /gene_synonym="IRK-2; IRK2; Kir2.2; MB-IRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            288..4427
                     /gene="Kcnj12"
                     /gene_synonym="IRK-2; IRK2; Kir2.2; MB-IRK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..1627
                     /gene="Kcnj12"
                     /gene_synonym="IRK-2; IRK2; Kir2.2; MB-IRK2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ATP-sensitive inward rectifier potassium channel 12;
                     inward rectifier K(+) channel Kir2.2"
                     /codon_start=1
                     /product="ATP-sensitive inward rectifier potassium channel
                     12 isoform 2"
                     /protein_id="NP_001254522.1"
                     /db_xref="CCDS:CCDS24805.1"
                     /db_xref="GeneID:16515"
                     /db_xref="MGI:MGI:108495"
                     /translation="MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRN
                     RFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIF
                     WVIAVAHGDLEPAEGRGRTPCVLQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVF
                     MVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNL
                     RKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDE
                     ASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEE
                     KNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEV
                     ATDRDGRSPQPEHDFDRLQASSAALERPYRRESEI"
     polyA_site      2291
                     /gene="Kcnj12"
                     /gene_synonym="IRK-2; IRK2; Kir2.2; MB-IRK2"
                     /note="major polyA site"
ORIGIN      
        1 cggcgcttct tgtcgcggga ctgagcgaca gaagccagcg gcgagcccgg agcgagcgcc
       61 gagtggctgg gggcgccgtc tgggcgtgcg cccccgacct atggctggct ggtggcaccc
      121 agcgcccggg agcgacgcgc ccctcggcgt ggagcgctcc caggggctgc gctgaggcgg
      181 cggcggcaac ggtggcaatg cagccagcgc agcggcggcc gggaccggga acggccgcgc
      241 ggagcttgga ggacgcggga cgcgccgcgc cccggccact gaccgagaag tgcccccgcc
      301 tggagtcagc ctgggggcag gccagggtcc accagacccc gggatgaccg cagccagtcg
      361 ggccaacccc tacagcatcg tatcatcaga ggaggacggg ctgcacctgg ttaccatgtc
      421 aggcgccaac ggttttggca atggcaaggt gcatacacgg cgccggtgcc gcaaccgctt
      481 cgtcaagaag aacggtcagt gcaacattga attcgccaac atggacgaga agtcacaacg
      541 ctacctggct gacatgttta ccacgtgtgt ggacatccgc tggcgctaca tgctgctcat
      601 cttctctctg gcctttcttg cctcctggtt gttgtttggc atcatcttct gggtcattgc
      661 tgtcgcccac ggggacctgg agccagccga gggccgtggc cgtacaccct gtgtgctgca
      721 ggtccacggc ttcatggcag cctttctctt ctccattgag acacagacca ccattggcta
      781 cgggctacgc tgtgtgactg aagagtgccc ggtggctgtc ttcatggtgg tggcgcagtc
      841 cattgtgggc tgcatcattg actccttcat gattggtgcc atcatggcca agatggcacg
      901 gcccaagaag cgcgcacaga ctctgctttt cagccataat gccgtggtgg ctctgcgtga
      961 cggcaagctc tgcctcatgt ggcgcgtggg caacctgcgt aagagtcaca tcgtggaggc
     1021 ccatgtgcgg gcccagctca tcaagcccag ggtcacagag gagggtgagt acatcccact
     1081 ggaccagatt gacatcgatg tcggctttga caagggccta gaccgtatct tcctggtatc
     1141 acccatcacc atcttgcacg agattgatga ggccagccca ctgtttggca ttagccgtca
     1201 ggaccttgag acagacgact ttgagattgt ggtcatcctg gagggcatgg tagaggccac
     1261 agccatgacc acacaggctc gcagttccta cctggctaac gagatcctgt ggggccaccg
     1321 ctttgagcca gtgctcttcg aagagaagaa ccagtacaag attgactatt cacacttcca
     1381 caagacctac gaggtgccat ctacaccccg ctgcagcgcc aaggacctgg tggagaacaa
     1441 gttcctcctg cccagcgcca actctttctg ctatgagaac gagctggcct tcctgagcag
     1501 agatgaggag gacgaggtgg ctaccgaccg ggatggccgc agccctcagc ccgagcatga
     1561 ctttgacaga ctgcaggcca gcagcgctgc ccttgaacgg ccctacagac gggagtcgga
     1621 gatttgaatg cccttggctt agatgcagca ccaccctgac cacaataggt cccatgtccc
     1681 ttgggggcct gcgtttgagc agagcaggcc gaaagcctcg ggtcacagac tcagtagcat
     1741 cttagtcttt ttcatgtttt ttcgcagtag cttgggaaag ttggcgggag cgtggatggc
     1801 caaaatgact ggctcacggc ctcggaggct gatgtatacc catgggcaag gaggtgactt
     1861 cttggggtag ggttgctcag gagttaggga ctctgctgga ggccttaggt gcaggtccca
     1921 gccccggtgg gaggaggctg tgtatgtaca cttcattggt ttttaacttg ggcaagactg
     1981 tttacaaaca aaaacaaaca aacaaacaat caaaaaaaaa acccacccca caaaacacaa
     2041 gaaaacatta acaggtggtt caatttttaa aattcctggt gtggggacag ttggaattct
     2101 actcagcgct gccaggcaag cagctggacg gagaaccctg agggttctca gaggtctgtt
     2161 ttctccctca cgtgggccag ggccttaagc gagttgtcat gcttaaggat gaatcaggct
     2221 gcttgtgacc atgttaacga ttctaataaa gtgtctggat gtctcttggg ggtggggccc
     2281 actagggtga atctggcttg ctcttctgtt tgatgttgtt gtacctcagc tgcctcaggg
     2341 ctgggtttag acaccccttc ccccaccccc actccgtact ccctgttgtg cttcccttcc
     2401 tatttagtcc caggtctcca ccccttgcag cgagggggca taggaaacac cagagtgggt
     2461 gcttcttaga aaaggacact caggaaatta gtatggggtc tgggtcccac atccccagga
     2521 agtgggtcag ccccacgtcc cccgctggac tcacagcttg agttgtgcct tagagactct
     2581 ggttgtctgg cttccgtcat ttctggtttt ctcagacttg tttaatcttt ccctactgcc
     2641 ctaagtccac tcatgaagct ccccaggccc ttctgagatt gcagaggctg cttgctcata
     2701 gccatgaccc tgcctgcttt tgtcccacac tgctggggca ggatgatgaa gccctgctgg
     2761 tcccagccag tcatctcctg ctggaaggca gagtctaggg gagaagagaa acacccccac
     2821 ccccacccct atcccgcacc agccccctgc caccatagag atagaccaga gaaatgtcat
     2881 aaaacaaaga attcctgttt agccttcaag gacctatggt ggaacttttc cttcctgagg
     2941 cccaagccgt ggagtcttcc tgtcgtgccc cgtcagggag acctgagata ggctggctcc
     3001 caggcacctt gcttcagatt ccatgctaat ttctaatcta ggagattccc ctaggacccg
     3061 ctgcagaggc atgggctcct ctgtggtcct ggccagacct ctgcccccaa atctcactga
     3121 ctgttctagc cttggccttg gggcctaagg gtgcattcag cctgggtgtg cttacctgcc
     3181 actcacctct ctatctagtt gtcgagtcta gggaagggca gagggtgggt cctggccact
     3241 gggtgccact gtgggagccg gggcacctgt cattctttct gagtaggtgg gcctgtatcc
     3301 agagcagggt ctaggggctc ctgctcccgg ctcatctcag tggcctgaag cattcaaaac
     3361 tctagccagc cccatcctcc tccctgccag aatggtccag tccctgggcc ctgctggaga
     3421 gcagctcaaa ctgcctttca tctctgccaa aggcaggcag atgctgtatt ttcaaattct
     3481 cctgccctct tttccccttt ttccctgtat ttatttattg cttgtgctaa agaccaaact
     3541 agccgccccc tttagtgcac ccaatggagg aagccactga ggggggagcc gtgtgcctgt
     3601 gccaggcagg aaagcttggc ccctgggaag tgtgtgagaa ggaaaaccag ggtgtgtgtg
     3661 cacgcatgtg cagacgtgct cacacgatcg tctccctgtg tgggaaactt ggtatgcagg
     3721 tatatggtat acacaggtgt ttatatacag gtagtctatg tctgaccaca ggtatgtgtg
     3781 tgcttgtttt tttttttttt ttttaaattt gggtaaggtg ggtttcatgc tcaccaacct
     3841 gaactggtca ggctggatgc tggccacaga catgatcagt ccgacttgag tccctgccct
     3901 gccctggtta tgcaggctgt acgacgcagt gtgcatcagt ctgaggagtc tggtggtaag
     3961 taagcctctg cagcagaccc tgtcggaggc catgggggcg tgtgtgtgtg tgtgtgtgtg
     4021 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgaacatgtg tgagggtgtg gaggtcccag
     4081 ggtgtgtgta tgtgaatgtc agtggcttgg gtgctgtgtg gagagagaga gagagagaga
     4141 gagagagaga gagagagaga gagagagaga gctgcacagg atgaagcagc tgccctgagc
     4201 ccatctggtg ctgtgcacct ggtccttgtc cagccgaggg cctgggggca gaagaacaca
     4261 ccatagggag gcccttgtat gggtgtctgc cctatgtgag gtcatagaat gttaaatcta
     4321 ttttaaacgg tgaaactgga gaattttcat gttattttca ataatagtgg tatctaaaga
     4381 tgtaggcaac aagactagac cacattaaat gcattttgat ctctttg
//
