LOCUS       NM_019659               3073 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus potassium inwardly-rectifying channel, subfamily J,
            member 1 (Kcnj1), transcript variant 2, mRNA.
ACCESSION   NM_019659
VERSION     NM_019659.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Niborski,L.L., Paces-Fessy,M., Ricci,P., Bourgeois,A.,
            Magalhaes,P., Kuzma-Kuzniarska,M., Lesaulnier,C., Reczko,M.,
            Declercq,E., Zurbig,P., Doucet,A., Umbhauer,M. and Cereghini,S.
  TITLE     Hnf1b haploinsufficiency differentially affects developmental
            target genes in a new renal cysts and diabetes mouse model
  JOURNAL   Dis Model Mech 14 (5) (2021)
   PUBMED   33737325
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Papanicolaou,K.N., Ashok,D., Liu,T., Bauer,T.M., Sun,J., Li,Z., da
            Costa,E., D'Orleans,C.C., Nathan,S., Lefer,D.J., Murphy,E.,
            Paolocci,N., Foster,D.B. and O'Rourke,B.
  TITLE     Global knockout of ROMK potassium channel worsens cardiac
            ischemia-reperfusion injury but cardiomyocyte-specific knockout
            does not: Implications for the identity of mitoKATP
  JOURNAL   J Mol Cell Cardiol 139, 176-189 (2020)
   PUBMED   32004507
  REMARK    GeneRIF: Global knockout of ROMK potassium channel worsens cardiac
            ischemia-reperfusion injury but cardiomyocyte-specific knockout
            does not: Implications for the identity of mitoKATP.
REFERENCE   3  (bases 1 to 3073)
  AUTHORS   Schiano,G., Glaudemans,B., Olinger,E., Goelz,N., Muller,M.,
            Loffing-Cueni,D., Deschenes,G., Loffing,J. and Devuyst,O.
  TITLE     The Urinary Excretion of Uromodulin is Regulated by the Potassium
            Channel ROMK
  JOURNAL   Sci Rep 9 (1), 19517 (2019)
   PUBMED   31863061
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3073)
  AUTHORS   Wu,P., Gao,Z.X., Su,X.T., Ellison,D.H., Hadchouel,J., Teulon,J. and
            Wang,W.H.
  TITLE     Role of WNK4 and kidney-specific WNK1 in mediating the effect of
            high dietary K+ intake on ROMK channel in the distal convoluted
            tubule
  JOURNAL   Am J Physiol Renal Physiol 315 (2), F223-F230 (2018)
   PUBMED   29667910
  REMARK    GeneRIF: WNK1, but not WNK4, is required for high potassium
            intake-induced stimulation of ROMK activity in convoluted kidney
            tubules.
REFERENCE   5  (bases 1 to 3073)
  AUTHORS   Kim,J.M., Xu,S., Guo,X., Hu,H., Dong,K. and Wang,T.
  TITLE     Urinary bladder hypertrophy characteristic of male ROMK Bartter's
            mice does not occur in female mice
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 314 (3), R334-R341 (2018)
   PUBMED   29092859
  REMARK    GeneRIF: ROMK is expressed in the urinary tract at both protein and
            mRNA levels. Significant enlargement and hypertrophy of the bladder
            may contribute to hydronephrosis in male ROMK KO mice.
REFERENCE   6  (bases 1 to 3073)
  AUTHORS   Yoo,D., Kim,B.Y., Campo,C., Nance,L., King,A., Maouyo,D. and
            Welling,P.A.
  TITLE     Cell surface expression of the ROMK (Kir 1.1) channel is regulated
            by the aldosterone-induced kinase, SGK-1, and protein kinase A
  JOURNAL   J Biol Chem 278 (25), 23066-23075 (2003)
   PUBMED   12684516
  REMARK    GeneRIF: SGK-1 phosphorylation of ROMK drives expression on the
            plasmalemma; because SGK-1 is an early aldosterone-induced gene,
            results suggest a possible molecular mechanism for
            aldosterone-dependent regulation of the secretory potassium channel
            in the kidney
REFERENCE   7  (bases 1 to 3073)
  AUTHORS   Lu,M., Wang,T., Yan,Q., Yang,X., Dong,K., Knepper,M.A., Wang,W.,
            Giebisch,G., Shull,G.E. and Hebert,S.C.
  TITLE     Absence of small conductance K+ channel (SK) activity in apical
            membranes of thick ascending limb and cortical collecting duct in
            ROMK (Bartter's) knockout mice
  JOURNAL   J Biol Chem 277 (40), 37881-37887 (2002)
   PUBMED   12130653
  REMARK    GeneRIF: Absence of small conductance K+ channel (SK) activity in
            apical membranes of thick ascending limb and cortical collecting
            duct in knockout mice a model for bartter's disease__
REFERENCE   8  (bases 1 to 3073)
  AUTHORS   Lorenz,J.N., Baird,N.R., Judd,L.M., Noonan,W.T., Andringa,A.,
            Doetschman,T., Manning,P.A., Liu,L.H., Miller,M.L. and Shull,G.E.
  TITLE     Impaired renal NaCl absorption in mice lacking the ROMK potassium
            channel, a model for type II Bartter's syndrome
  JOURNAL   J Biol Chem 277 (40), 37871-37880 (2002)
   PUBMED   12122007
  REMARK    GeneRIF: knockout mice have impaired renal NaCl absorption; a model
            for type II Bartter's syndrome
REFERENCE   9  (bases 1 to 3073)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 3073)
  AUTHORS   Boim,M.A., Ho,K., Shuck,M.E., Bienkowski,M.J., Block,J.H.,
            Slightom,J.L., Yang,Y., Brenner,B.M. and Hebert,S.C.
  TITLE     ROMK inwardly rectifying ATP-sensitive K+ channel. II. Cloning and
            distribution of alternative forms
  JOURNAL   Am J Physiol 268 (6 Pt 2), F1132-F1140 (1995)
   PUBMED   7611454
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132114.4 and CF168846.1.
            
            On Dec 4, 2009 this sequence version replaced NM_019659.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (2) has a shorter N-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK085262.1, CF168846.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               AC132114.4         144137-144243
            108-763             CF168846.1         1-656
            764-3073            AC132114.4         168607-170916
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 17.69 cM"
     gene            1..3073
                     /gene="Kcnj1"
                     /gene_synonym="Kir1.1; ROMK; Romk2"
                     /note="potassium inwardly-rectifying channel, subfamily J,
                     member 1"
                     /db_xref="GeneID:56379"
                     /db_xref="MGI:MGI:1927248"
     exon            1..196
                     /gene="Kcnj1"
                     /gene_synonym="Kir1.1; ROMK; Romk2"
                     /inference="alignment:Splign:2.1.0"
     exon            197..3073
                     /gene="Kcnj1"
                     /gene_synonym="Kir1.1; ROMK; Romk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..1336
                     /gene="Kcnj1"
                     /gene_synonym="Kir1.1; ROMK; Romk2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ATP-sensitive inward rectifier potassium channel 1;
                     inwardly rectifying potassium channel ROMK-2;
                     ATP-regulated potassium channel ROM-K; inward rectifier
                     K(+) channel Kir1.1; potassium channel, inwardly
                     rectifying subfamily J member 1"
                     /codon_start=1
                     /product="ATP-sensitive inward rectifier potassium channel
                     1 isoform 2"
                     /protein_id="NP_062633.1"
                     /db_xref="CCDS:CCDS22953.1"
                     /db_xref="GeneID:56379"
                     /db_xref="MGI:MGI:1927248"
                     /translation="MFKHLRRWFVTHIFGRSRQRARLVSKDGRCNIEFGNVDAQSRFI
                     FFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPC
                     VENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAIL
                     AKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITP
                     EGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVV
                     FLDGTVESTSATCQVRTSYIPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETP
                     HCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM"
     regulatory      3044..3049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnj1"
                     /gene_synonym="Kir1.1; ROMK; Romk2"
                     /note="hexamer: AATAAA"
     polyA_site      3073
                     /gene="Kcnj1"
                     /gene_synonym="Kir1.1; ROMK; Romk2"
                     /note="major polyA site"
ORIGIN      
        1 tgcatataga tgagtcagga gccagtcaaa gctgacccac agacataaaa cccaaggtac
       61 tgggcacctt tagctggggc atccaaactc tcgaatcaaa ggtgcaaggg actttctcac
      121 atcaagcacc tgactgctct cttggggacc aagaaaatga acgtttgtct ccacgtttac
      181 cccagcaatc catgagatca gggcactgac agaaaggatg ttcaaacatc ttcgaagatg
      241 gtttgtcact cacatatttg ggcgttctcg gcaacgagca aggttggtct ccaaagatgg
      301 aaggtgtaac atcgagtttg gcaatgtaga tgcacagtcg aggtttatat tctttgtgga
      361 tatctggaca actgtacttg acctgaaatg gaggtacaaa atgaccgtgt tcatcacagc
      421 cttcttgggg agttggtttc tctttggtct cctgtggtat gtcgtagcct atgttcataa
      481 ggatctccca gagttctacc cacctgacaa ccgtactcct tgtgtggaga acattaatgg
      541 catgacatca gcctttctgt tttctctaga gacccaagtg accataggtt acggattcag
      601 gtttgtgaca gaacagtgtg ccactgccat ttttctgctt atcttccagt ctattcttgg
      661 agtgatcatc aattctttca tgtgtggtgc catattagcc aagatctcta gacccaaaaa
      721 acgtgcaaag accattacat tcagcaagaa tgcggtgatc agcaaacgtg gggggaagct
      781 ctgtctcctc atccgagtag caaatcttag gaaaagcctt ctgattggca gtcacatata
      841 tggtaagctt ctgaagacta ccatcacacc tgaaggagag accattattt tggatcagac
      901 caatataaac tttgtagttg atgctggcaa tgaaaatttg ttcttcattt ccccactgac
      961 aatctaccac attattgacc acaacagccc tttcttccac atggcggcag aaactctttc
     1021 ccaacaggac ttcgagttgg ttgtcttttt agatggcaca gtagaatcca ccagtgcaac
     1081 ctgccaagtc cgcacatcat acatcccaga agaggtgctt tggggttacc gttttgttcc
     1141 catcgtatcc aagaccaagg aagggaaata ccgagtggat ttccataact ttggtaagac
     1201 ggtggaagtg gagacccctc attgtgccat gtgcctctat aatgagaaag atgccagggc
     1261 caggatgaag agaggctatg acaaccctaa ctttgtcttg tcagaagttg atgaaacaga
     1321 cgacacccaa atgtagcagt ggcttttcta cctacaaaga gtctctcaag gacctaagtg
     1381 ctggctgtgt tcagaagcat cctgcagttg ggggactgaa agcaggatga gaacccaaga
     1441 agtctaccag cagagtcacc ccctcagccc catggctatg attctagaag actcgtagct
     1501 ctaaagtctg cagcagtaag catacggtgg aagcatgtga atgaacctgg cgtgtggaag
     1561 ccaaaaggag ctcacttgga tcttcactct gactgtgtaa gctcgtacag tgttgatgga
     1621 aacaaagtca tccattgaca tgtactggag aaaagttagg agctttagaa aacttcagga
     1681 gctagccata tttcctgttt gattctatgg atgagaaaga tatcacattt tatcttaaag
     1741 tagactttta tcaacggaaa atctgtcttc tgagttggga agtgagccaa ccagtcactg
     1801 acaataagag cctgtcatac acagaatcac taaaggctct aacttcttaa gctctgctgt
     1861 ttgaagcctt tgtctgagtc tgggtccatg tttcagaagg ggtaaggtgg gcatccagtg
     1921 actgcacctt tctcaaccca aggtacagaa gaacagaaag cctcaaccaa tttcataact
     1981 aactcagacg ctgcagccca tataggaatg gacctgaatg atttccggtg gtgcaatcaa
     2041 tggaggccaa gcccattcct tagactaaat gaacattctc ttgcaaagga gagtgtttga
     2101 gctaaaagtg ttggtgaaga ggctgaagag atgacattct ttgtaagttc atgctgcgca
     2161 cgcatgaaga cctgggtctg aacctccaac aatcacatac aaagcttgac ttagtaggat
     2221 gctccccctc cccaatccta gtactgggga ggtagggata ggaggatgtc tcaagttttc
     2281 tggacagctg aatgggtgag gtaccagttc agggagagac tacaaaaata aggtgaaggg
     2341 gctggcggga agactctggt taaaagcacc gtggctgatc ttccagagaa cctaggtttg
     2401 gttcctggca cagtacttca taactatcta taactccagt ttcagggcat ctgatgctct
     2461 tttctggccc ctatgagcac caggtgcaca tgcgagcaaa acactataca catacgatta
     2521 aaaagataag gtagagaatg actgaagaag aaatcttacg tcaacttctg atctacacac
     2581 acacacacac acacacacac acacacacac agagcatagt gatgtttgtt tcagtattca
     2641 gtgggtaggt tatagggcca caggtctggt aaaagaggtt ttattccttc ctctctcaag
     2701 atggtgctga ggactgggaa agcagtcagc ctggagtgtg tatgatgcta acaccgaatg
     2761 gttcttgtgt ttctctgcct agtggttgaa ctgcaattct aggtatgaag gagaaaggaa
     2821 cgagatacag caccacagtc aggatgctgc tgctttcttg tctctaaagg ccatccctac
     2881 atcttccaaa gggaacaagt tagcaggaga atttacttcg gaggaaggtt ctaatgtctt
     2941 gtttgttcat ttccacatct gcatcattct gatttcacct tgggggaaaa aaagtagaat
     3001 tcaaagctgt tcaaaaatca aaatatcatt ttagtatgtg attaataaat ttctgaaact
     3061 gtatgagcaa aaa
//
