LOCUS       NM_001098528           15494 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus potassium voltage gated channel, Shab-related
            subfamily, member 2 (Kcnb2), mRNA.
ACCESSION   NM_001098528 XM_006495595 XM_976837
VERSION     NM_001098528.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 15494)
  AUTHORS   Fan,J.J., Erickson,A.W., Carrillo-Garcia,J., Wang,X., Skowron,P.,
            Wang,X., Chen,X., Shan,G., Dou,W., Bahrampour,S., Xiong,Y.,
            Dong,W., Abeysundara,N., Francisco,M.A., Pusong,R.J., Wang,W.,
            Li,M., Ying,E., Suarez,R.A., Farooq,H., Holgado,B.L., Wu,X.,
            Daniels,C., Dupuy,A.J., Cadinanos,J., Bradley,A., Bagchi,A.,
            Moriarity,B.S., Largaespada,D.A., Morrissy,A.S., Ramaswamy,V.,
            Mack,S.C., Garzia,L., Dirks,P.B., Li,X., Wanggou,S., Egan,S.,
            Sun,Y., Taylor,M.D. and Huang,X.
  TITLE     A forward genetic screen identifies potassium channel essentiality
            in SHH medulloblastoma maintenance
  JOURNAL   Dev Cell (2025) In press
   PUBMED   39862856
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15494)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 15494)
  AUTHORS   Stewart,R.G., Camacena,M., Copits,B.A. and Sack,J.T.
  TITLE     Distinct cellular expression and subcellular localization of Kv2
            voltage-gated K+ channel subtypes in dorsal root ganglion neurons
            conserved between mice and humans
  JOURNAL   J Comp Neurol 532 (2), e25575 (2024)
   PUBMED   38335058
REFERENCE   4  (bases 1 to 15494)
  AUTHORS   Kirmiz,M., Palacio,S., Thapa,P., King,A.N., Sack,J.T. and
            Trimmer,J.S.
  TITLE     Remodeling neuronal ER-PM junctions is a conserved nonconducting
            function of Kv2 plasma membrane ion channels
  JOURNAL   Mol Biol Cell 29 (20), 2410-2432 (2018)
   PUBMED   30091655
  REMARK    GeneRIF: these findings demonstrate a conserved in vivo function
            for Kv2 family members in remodeling neuronal endoplasmic
            reticulum-plasma membrane junctions.
REFERENCE   5  (bases 1 to 15494)
  AUTHORS   Kirmiz,M., Vierra,N.C., Palacio,S. and Trimmer,J.S.
  TITLE     Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins
            Defining Endoplasmic Reticulum-Plasma Membrane Junctions in
            Mammalian Brain Neurons
  JOURNAL   J Neurosci 38 (35), 7562-7584 (2018)
   PUBMED   30012696
REFERENCE   6  (bases 1 to 15494)
  AUTHORS   Johnston,J., Griffin,S.J., Baker,C., Skrzypiec,A., Chernova,T. and
            Forsythe,I.D.
  TITLE     Initial segment Kv2.2 channels mediate a slow delayed rectifier and
            maintain high frequency action potential firing in medial nucleus
            of the trapezoid body neurons
  JOURNAL   J Physiol 586 (14), 3493-3509 (2008)
   PUBMED   18511484
  REMARK    GeneRIF: It was concluded that Kv2.2-containing channels have a
            distinctive initial segment location and crucial function in
            maintaining AP amplitude by regulating the interspike potential
            during high frequency firing.
REFERENCE   7  (bases 1 to 15494)
  AUTHORS   Harrell,M.D., Harbi,S., Hoffman,J.F., Zavadil,J. and Coetzee,W.A.
  TITLE     Large-scale analysis of ion channel gene expression in the mouse
            heart during perinatal development
  JOURNAL   Physiol Genomics 28 (3), 273-283 (2007)
   PUBMED   16985003
REFERENCE   8  (bases 1 to 15494)
  AUTHORS   Fink,M., Duprat,F., Lesage,F., Heurteaux,C., Romey,G., Barhanin,J.
            and Lazdunski,M.
  TITLE     A new K+ channel beta subunit to specifically enhance Kv2.2 (CDRK)
            expression
  JOURNAL   J Biol Chem 271 (42), 26341-26348 (1996)
   PUBMED   8824288
REFERENCE   9  (bases 1 to 15494)
  AUTHORS   Lock,L.F., Gilbert,D.J., Street,V.A., Migeon,M.B., Jenkins,N.A.,
            Copeland,N.G. and Tempel,B.L.
  TITLE     Voltage-gated potassium channel genes are clustered in paralogous
            regions of the mouse genome
  JOURNAL   Genomics 20 (3), 354-362 (1994)
   PUBMED   8034307
REFERENCE   10 (bases 1 to 15494)
  AUTHORS   Wei,A., Covarrubias,M., Butler,A., Baker,K., Pak,M. and Salkoff,L.
  TITLE     K+ current diversity is produced by an extended gene family
            conserved in Drosophila and mouse
  JOURNAL   Science 248 (4955), 599-603 (1990)
   PUBMED   2333511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166075.2 and AC126049.3.
            
            On or before Jun 20, 2019 this sequence version replaced
            XM_006495595.3, NM_001098528.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.33628.1,
                                           SRR7652917.340997.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-560               AC166075.2         157280-157839
            561-1228            AC166075.2         182718-183385
            1229-15494          AC126049.3         179221-193486
FEATURES             Location/Qualifiers
     source          1..15494
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 4.5 cM"
     gene            1..15494
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="potassium voltage gated channel, Shab-related
                     subfamily, member 2"
                     /db_xref="GeneID:98741"
                     /db_xref="MGI:MGI:99632"
     exon            1..560
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /inference="alignment:Splign:2.1.0"
     exon            561..1228
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /inference="alignment:Splign:2.1.0"
     CDS             650..3373
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="voltage-gated potassium channel subunit Kv2.2"
                     /codon_start=1
                     /product="potassium voltage-gated channel subfamily B
                     member 2"
                     /protein_id="NP_001091998.1"
                     /db_xref="CCDS:CCDS48222.1"
                     /db_xref="GeneID:98741"
                     /db_xref="MGI:MGI:99632"
                     /translation="MAEKAPPGLNRKTSRSTLSLPPEPVDIIRSKTCSRRVKINVGGL
                     NHEVLWRTLDRLPRTRLGKLRDCNTHESLLEVCDDYNLNENEYFFDRHPGAFTSILNF
                     YRTGKLHMMEEMCALSFGQELDYWGIDEIYLESCCQARYHQKKEQMNEELRREAETMR
                     EREGEEFDNTCCPEKRKKLWDLLEKPNSSVAAKILAIVSILFIVLSTIALSLNTLPEL
                     QENDEFGQPSDNRKLAHVEAVCIAWFTMEYLLRFLSSPNKWKFFKGPLNVIDLLAILP
                     YYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNE
                     LGLLILFLAMGIMIFSSLVFFAEKDEDATKFTSIPASFWWATITMTTVGYGDIYPKTL
                     LGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIV
                     SMNLKDAFARSMELIDVAVEKAGESANTKDSVDDNHLSPSRWKWARKALSETSSNKSY
                     ENKYQEVSQNDSHEHLNNTSSSSPQHLSAQKLEMLYNEITKTQPHSHPNPDCQEQPER
                     PCVYEEEIEMEEVICPQEQLAVAQTEVIVDMKSTSSIDSFTSCATDFTETERSPLPPP
                     SASHLQMKFPTDLPGTDEHQRARAPPFLTLSRDKGPAAREAAVDYAPIDITVNLDAGA
                     SHGPLQPDSASDSPKSSLKGSNPLKSRSLKVNFQENRASAPQTPPSTARPLPVTTADF
                     PLTTPQHMSTILLEEALPQGQPPLLEADDSAHCQGPSKGFSPRFPKQKLFPFSSRERR
                     SFTEIDTGEDEDFLDLQRSRPDKQADPSPNCLADKPGDARDSLREEGCVGSSSPQNTD
                     HNCRQDIYQAVGEVKKDSSQEGYKMENHLFAPEIHSNPGDTGHCPTRETSM"
     exon            1229..15494
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5929..5934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="hexamer: ATTAAA"
     polyA_site      5958
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="major polyA site"
     regulatory      6016..6021
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="hexamer: AGTAAA"
     polyA_site      6044
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="major polyA site"
     regulatory      7471..7476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="hexamer: AATAAA"
     polyA_site      7492
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
     regulatory      15477..15482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="hexamer: AATAAA"
     polyA_site      15494
                     /gene="Kcnb2"
                     /gene_synonym="9630047L19Rik; Kv2.2"
                     /note="major polyA site"
ORIGIN      
        1 gcgcggagcc agcgtcgctg ctgcacagac acacacccac cgagctccac cgagtgcccc
       61 tgagcacccg ccgcccccgc tggccacagc cgctcctccg gcagcgggat ccagcgccaa
      121 gtccagggct ggtgcggcgc gcagagaacc tcttgatttt ttttccccct tcactccagc
      181 cctctctggt ctgggcggcg gcggcggcga cagggggatg ggggctccac gcggggaaag
      241 gcgggtgacg atcgaggaag attattgttc tttttaaagg atgccttcgc ttcttaccaa
      301 tgcggcttct gtgagccctt ttactagaga ctgcgaaccg gagtgagata tgtaaagaag
      361 gaaggaaagc tgcctggagc tccagccagc cgggcagacc ctcctctctg tggaggggag
      421 ggggatttcc aacgacacat cattggcaaa agggaccttt gcaagagagg agtcctctga
      481 gggagcaggg aatcccacag ccccctgtgg cacaggagcc agagctgctg gctgagcaga
      541 gctgggtccg tgggagctag ctgatgtggc gatgcctatt ccagaggaat cctgcttcag
      601 gtgacttcct ttctaaggac tctagctctg tggctctgtc cagttcaaaa tggcagaaaa
      661 ggcaccacct ggcttgaaca gaaagacatc caggtcaaca ctttctcttc ctccagaacc
      721 tgtggacatt atccgaagca aaacatgctc tcggagagtt aagatcaacg tagggggcct
      781 taaccacgaa gtcctgtgga gaactctgga caggcttccc cggacacgcc tggggaagct
      841 ccgcgactgt aacactcacg agagtctcct ggaggtgtgc gatgactaca accttaacga
      901 gaatgagtat ttcttcgacc gacatcctgg agcctttaca tctattctaa acttctaccg
      961 gacggggaag ctccacatga tggaggaaat gtgtgcgctc tcttttggcc aagagctgga
     1021 ttattggggc atcgatgaga tctacctaga gtcctgctgc caagccagat accatcagaa
     1081 gaaagagcag atgaacgaag aactgaggag ggaggcggag accatgcgtg agcgtgaggg
     1141 tgaggagttt gataacacct gctgcccgga gaaacggaag aaactttggg acttgctgga
     1201 gaaacctaac tcgtccgtgg ctgcaaagat cctggccatc gtgtctatcc tgttcatcgt
     1261 tctttccacc attgctctgt ctctcaacac acttccagag ctgcaagaaa acgacgagtt
     1321 tgggcaaccc agtgacaacc ggaagttggc acacgtagag gctgtgtgca ttgcctggtt
     1381 taccatggag tatcttttac gattcctgtc ctcaccgaat aaatggaaat tctttaaagg
     1441 cccgctgaat gtcattgacc ttctggccat cttgccatac tatgtaacca tcttcctcac
     1501 cgagtctaac aaaagtgtgc tgcagttcca gaatgttaga cgagtggtcc agatcttcag
     1561 aatcatgcgt atcctcagga tactgaaact cgccagacac tcaacgggcc tgcagtctct
     1621 gggcttcaca ctcaggcgaa gttacaatga gttaggttta ttaatattat ttctggccat
     1681 ggggataatg atattttcca gcttggtgtt ttttgctgag aaagatgaag atgctaccaa
     1741 attcaccagt atccctgcat cattttggtg ggctaccatc accatgacca ctgtgggcta
     1801 cggtgacatt taccctaaaa cactgttagg aaaaattgtg ggtggtctct gctgcattgc
     1861 aggagtcctg gttattgccc ttcctatacc catcattgtg aacaatttct ctgagttcta
     1921 taaggagcaa aaacgccagg agaaagctat taaaaggaga gaggctcttg aaagagccaa
     1981 aagaaatggc agcatcgttt ctatgaactt aaaggatgcc tttgctagaa gtatggaact
     2041 gatagacgtg gctgtggaga aggctggaga gtcagccaat accaaggact cagtagatga
     2101 taatcacctg tctccaagcc ggtggaagtg ggccaggaag gcgctgtcag aaacgagctc
     2161 caacaaatct tacgagaata agtaccagga ggttagccaa aatgactccc acgaacatct
     2221 gaacaatact tcttcctcca gcccacagca tctgagtgcc cagaagctgg agatgctata
     2281 caatgaaatc accaagacac agcctcattc ccacccgaat ccagactgcc aagaacagcc
     2341 tgagaggcca tgtgtgtatg aggaggagat agaaatggaa gaggtgatct gcccacagga
     2401 gcagctggct gtggcacaga ccgaggtcat cgtggacatg aagagcacct ccagcattga
     2461 cagcttcacc agctgtgcca cggacttcac agagactgag agatcacccc tgccaccacc
     2521 ctctgcctct catttgcaga tgaagttccc cacggacctc ccaggaacag atgagcacca
     2581 aagagccagg gcacctccat tccttacact aagcagagat aaggggcctg ctgccaggga
     2641 ggctgcagtg gattatgccc caattgacat aaccgtgaac ctcgatgctg gggcttccca
     2701 tggtcccttg caacctgaca gtgccagcga cagccctaag agctcgctga aagggagcaa
     2761 tcccctcaag tcaagatccc tcaaagtgaa ctttcaggaa aacagagcca gtgcaccaca
     2821 gaccccgccc agcacagcca ggccactgcc agtaaccaca gctgactttc cgctcaccac
     2881 tccccagcac atgagtacca ttcttctaga agaagccctc ccccagggac agcctccctt
     2941 gctggaggct gatgattcgg cacactgtca gggaccatcc aaaggtttct ccccacgatt
     3001 tcccaagcaa aaactgtttc ctttctcttc tagagaaagg aggagcttca ctgaaataga
     3061 cactggagaa gacgaagact tcttggacct ccaaaggtca agaccagaca agcaagcaga
     3121 tcccagcccc aactgcctag cagataagcc tggcgatgcc agagactctt taagagaaga
     3181 gggctgtgtg ggttcctcct ctccccagaa cacagaccac aactgtaggc aagacattta
     3241 ccaggctgtg ggtgaagtca aaaaggacag tagtcaagaa ggttacaaga tggagaacca
     3301 cttgtttgcc ccagaaattc attccaaccc aggagacaca ggtcactgtc ccactcgtga
     3361 gaccagcatg tgacaaaagc aataaaagaa aaagaaaaaa aaactgtttc ttaaaaatgc
     3421 gattaataat gccgtgaact aaaatttgag aaacccctcc ccctaatacc agtgcataaa
     3481 atattatttt ttgcatggca tgaacagtat agtttaagta ctgtttaaat aaaaccgagt
     3541 cctgaatagc gagcaaataa agtgctctct taggaacctg tgctcagcca tgtctgacat
     3601 ttttgctcct ttcatttcca atagcaggca ggttttggag tttcaactct tacctcccag
     3661 tgaactttag aatttgcaca tgaaaggatg aaatgttgtt gcatgtgttc atgcttacgg
     3721 ggagaaaggt gctttcagct tgcaaacttt gcataacttt gtaaataggc ctggatgtaa
     3781 atgtgcctca aacatgtgga cacgccattt ctgagacaca ggtacctcct tcacagctac
     3841 tgcctgaagg agctgtccag acttcctgtt gcaaaattgt aacctcttct taacccattt
     3901 tgtgtatctt gctttggatt ataaagctct taggtttgtt taaaggaata cagtattttt
     3961 atttttagga tataatttgg agggtatatt tatatgtatt ttcacttatt tgtgatacag
     4021 ttgacagggt atttttcttt tcttcttttg ctttgttgtt gcaattgtga tgttcttata
     4081 aatcatggaa attagtaaga tttaatgacc agatatcaag tatttggaat ttaagcttga
     4141 gcttttacct ataaactaaa tatcatgtca tagattatta cagaaattca agtttacaca
     4201 aagctgaaaa tgtacagaaa gttctattat aaaataaaac ttttaattgg tactccacat
     4261 tcagaaatca cctttaaaac aatgtgtgct tcagcactcc aaacatggat ctgctgtcca
     4321 acatacaaat aaagagctgc taaaaatgga aattccataa aacgactttt aagaaaataa
     4381 aagtagaacc cccataagag acaaagctgg tcccagcatg agataccgtg ctaggagccc
     4441 aggtctgggc cactagggta gagacctagg atgacagtac acggtgcagg aaaatgtcct
     4501 tgtagacgta agtccttccc aagcatggcc agaacactcg gtaactgcag cagccctaca
     4561 agctttctca tgtttccaag cctggaactt tccttgctaa aagtcatgcc aggcattttc
     4621 ctactgagaa caccaaatcc agaggcactg ggcactgggg gttaccctgc tcaccagact
     4681 caccttgctt tgtggaaatc aaagctccag ctactgatga gaagctgccg gctgtgcagt
     4741 ctccaggatg tctccaacct ggtgggatct cacgtgcatc caaagggaaa cagtccaaat
     4801 tatttctaag cctccttaag atctcaaatt aatgatctct tagtttcctg gcaatgctca
     4861 ggttttgcgg cagcaaaatg catttcgtgc atgtgtgtat atagttcagg gaaatgggga
     4921 aactgccagc acaaactaca aagtgtattg tttaaggggg gaaaaaatct aaattttgtt
     4981 ctttattctt taaaggaaaa atgtttacaa aaatagccca aatatttaga atctaatata
     5041 agtcttgaat atttcttaac aggatctggt cattaaatct caaataagga aatgaatttt
     5101 gattttagtt aaaattcagg cagtcctgat attaagaata atttgaagta ttcttttgtc
     5161 ccattgtgct tagaatttct taatggtttt tatgtttgtt tcaaaacaaa acctttaaga
     5221 gtttatactc attaatcatt gatatttata ttatgcatac ttctttttgt gtctaaaaac
     5281 taagtgttga tcagtttttg taaatctaga gtatttgaat tttctatata tagttttaca
     5341 aagtcaagca ggtctatcta ttgttaaatg aatagcaaat aagttacaaa gatcattttc
     5401 ttcatacagc ctcatgaagc agttattttc taaggtctct tattaatgaa tctatttttt
     5461 tttattcatt tggcttattg ccatatgacc agtgtctcac gttccatgtc tgtcaggcct
     5521 tctatttagc accccgctcc ataatgctta tgatggggac ccttacccag gctctgctgt
     5581 tttccctgga agcatttttc tgaaacctct ccctttcatt ctcagagatt gctgtttatt
     5641 tcatttttat attttaaaga attgttttta agtccttgga aatttcatag tttgagtctt
     5701 tctagttcct ctgagacaaa cgtcttcagg taggagaaac aaatgaacac tttcctctaa
     5761 gaaatcttca actgtgctgt tttacaagga ggtatttccc agaatgcacc tatgagtccc
     5821 ttctaaactt tggcaaatgg cgtcaaactc agaggagaac atgtaagtct ggaaagacct
     5881 ggatacatct gtcaagcgga gttctttttg aagtcttgta tgttctacat taaaagtcca
     5941 tggccatgct gcaacttact ccatgacatg tatgtttgag aattttaaaa tgctgtttta
     6001 tattatgtat caactagtaa aactgacagt cttataactt caaatacctc ttttgtatac
     6061 cttggaaaac atcacccaag tttaggtagg actacctgaa ggggctattg gaaggttgtg
     6121 cttccaagag ttcatttcac cattagctag ctcagtgacc aaggataaat tccaatgaga
     6181 aagattattt gaatggcttc aaaattacat gatccttcta atggcaaagc tgtttctgct
     6241 gggcctctta accagtgcaa attgcccaag acgtttttaa tttcaaagat aagagcattg
     6301 aaacaggtat gcattgagtc ctgggatact gctcaatggt agaggacctt cctagcatgt
     6361 atgacaccct gattaatctc cagcactgta gaagacaaaa acgggggaga caggaagaaa
     6421 tgagggacct atctgtaggt ccacacatac accaagtgga tccctgaaat tgacctaatt
     6481 gttctctaaa catttcacta gaaatgctct cactctgaca cagtcaactg ctttgaagca
     6541 ataccatgat gagaccagat ctaataggaa aagagagttg atgaccagtg tccattccca
     6601 tattctctta ttaacattct tattcccatg ctggccacgg aaattttaca ttttacaatt
     6661 tattttatct tgaaattata aagatttgga gggggtagct taggtatgtt ctttctcgac
     6721 agtgcccaga attatcagta aagatagaga aatctgctct tagttctatt ccacccaggt
     6781 cactggtgag tggccgacct tgacctcaca cacaccatct ggctctaaat atggcatcct
     6841 aatgtatcac tggaaagggt gttcaatggc taaaaccatg taaatagaaa gcatattgct
     6901 aatatgaatg ttttatttta aagtagcaaa tctgtggggt agtaagagct aagaaccaag
     6961 ttaggcactt atgtttgcca gttgagaatt ctgtcaatag catacatggc ctttctctca
     7021 agagaagact ccactatttg tgatgtgagg tggcacacac atgaggcaga agtctataga
     7081 aagccacttg atcttcctga acaaaatcag acagagataa atccaattca gactaaaatg
     7141 catcatttat tgaatgtcat cactattttg ctgtttactc attcaatcaa ctctagatga
     7201 atatctccct cttccatgcc aaccacacaa gggttcagtt ctcttccctg acaaggacag
     7261 attcagctct tagcctcatg aagccttctg acactcttgg ctggctgcac catttatggg
     7321 aagctgaaga tatttgttgt tactgcaaaa aggtcaccta tcaggcagaa aaaaaaaaaa
     7381 tcacccaatg atctcatcag agagtcatag aacatacata aattatctta cttttccaaa
     7441 gacctatttt tttaagaaat agatgaactg aataaagcct aatttataat catgtttgat
     7501 caagtttatt tttcatgctt gaaaaagcaa attgttgatc tgattacatg aggtcataaa
     7561 ctatctttgt tatctggtaa attaaaaaat aatattttat ggttggtagt gaaccttagt
     7621 ggtagagtat tttgtctgca ttaagccccg ggtcaatcac cagtaccgaa acaaaaaaaa
     7681 cattatttta atttcttatc aaaatagcta ataataatct gtcacaagta actaaagcaa
     7741 aatttaatac tagtaagaaa ggactaaaaa tttttacatt tatgaataaa ttttaaaaca
     7801 gcattttagt gcttttcttc ctgcagtctc ttggcaacat tacaagaata tatacctcac
     7861 tcaaccctct aaattcttaa catttagaag tcaacattca aaagtctctt ggtttggctc
     7921 aaaaatttaa tcactaggtc tatggagatt tgaaagtata tttggttatt ttgccactgc
     7981 aatgtcttcc tcaaagtaac aaatagataa atagcagaca aatagaagat agataagtag
     8041 atagacagac agacagatag atagatagat agatagatag atagatagat agatagatag
     8101 atagatagat ggaaaaaaag agaaaagaga aatgggtttg gtggcacatg ctctacccta
     8161 gcacttggga ggttgaaaca ggagaacccc gagtctaaac acagctgtca ctacatagtg
     8221 aatttcagat tatctcagct acatagtaat aacctctcca aaaaggagga ggaggactag
     8281 gaagaggaag ggtaaaagaa gaggaagagc agcagcagct ggacatagtg gtatacactg
     8341 gaatctctac acgaaggagg cagaggtagg ctaatcttga ggtcaaggcc agcctagtct
     8401 atatacgacg ttccagccca tgcagataca cagtgagaca ctgtctttaa agaaagagaa
     8461 agaaaaacag agaggggaag tagagggaga ggaaagaaac taaaagaaac tctgatatgt
     8521 aatagaaatg aaaatgttta aagaccagtt tctgctaaga agaggaaggc cttggctttg
     8581 cagttctggt gagcagttgt ccatactgta gttaacactg ggctgggctg catacttccc
     8641 atggtttcaa cagccatgtc ctccacttgc ctttgtaaag tacaatccca tgaatccttc
     8701 ttcaccacca tcattaacat gcattacttt cctcagtaac tctataaagg taatatatat
     8761 atatatatat atatatttca aagccagcaa aagacaaaaa cataaaagaa gaaagatttt
     8821 taaatgtaag taattggtat tcttatttgg tgtgtatacc tttataaatg ttgtacttat
     8881 aaaaatatac atgtataaaa tttagatact tagaggatac gtgaagaata aaatcagaag
     8941 ttagttctta ccaccaaaca gttctagatt gttctgttga catataacaa agctttcctg
     9001 agcacctata gtttgcttga aggtaacaca tacagtcact tagtccagct tacagcctag
     9061 caaacatttt tttaaaatga ttatgttccc atgtgaaatc cattttcaca gagtaggcta
     9121 gaaatagcgt gacaagatgt ctatgtctgt tgtctaagat ctccaaagtt cttttccatt
     9181 tccttagatg caaggaaatt ccaccacata atttctaaac attctagctt aaaattacaa
     9241 taaatattgg acatttagtg acagtgtgaa gatattttca acttcaaaat gagaaagatg
     9301 aatgaaaaat atattatagc caaaaatcat gtctattcat taaaaatgac ttaaaaacaa
     9361 acagaaacta ggaactctgt ctagatatga tcccacattg acctcagaat cacaatcctc
     9421 atgtctcagc tgtctatgaa atgatctttt tttttttaca ttttgtttca tattttcagc
     9481 cctctcctct agttaagcca acaccaccta ctgctttggc aaaagccaaa tgctggtctg
     9541 agcagtgcag attctgtcca tctgcagtgg gacctactga caaagagatg gagggaccca
     9601 ctgacaacat gatgatgatg ggacacattg atgagatggt gggagagttt ttcttacagt
     9661 tatttttcat gtttttcaag ctgaaacaga tttgaggtgc ttttcttaca gttatttttc
     9721 atgtttttca agctaaaatg gatttgaggt cgcataacaa ggcagaacaa cagcctttca
     9781 gaatgaagac acagaattct gacaatcaga ggcaaaagac tcagaggtgt atcatactat
     9841 ccaccctcct gccttccttc accttaaatc tcttttggtg ctcagtgcaa gaaaaaatga
     9901 attatttgac aattgtgcat agatgtaaca gtcaaggcca tcccccacta ctagcccttc
     9961 agtccaactc cttgatctct gccactattg cctcccgatt ccatgtattc cgcaaaggat
    10021 gggattctat ttgcccagaa aaggtggttg gtaaccagaa gtaaggacaa ttgctctgcc
    10081 cttcacatgc tgtgcatgtt ctctgcatgc agtcagaaac aactaggctc ctgacatccc
    10141 tgtggccaca gctctttttt ctctttgctg tgtccctctg tgtttgcaac gctgtccacc
    10201 atgctttcag ggcaccccat gtgctgcccc aaaacacaca cacaaatctc gtttggctac
    10261 atcttgacca atgctccaga tctctgctgg actgtacaga atgtgtcact ttgacatccc
    10321 tgccactatg ggtagctagg caagaactcg aaatactgtg tgtgtggggg gggggggggg
    10381 aatcatagat gtaaagacaa tcctggcttc taatctgtcc aacaactatt tcatatagct
    10441 tctacttgaa gactatggca cggcccctta tatgaccctg gtagaaaaag gagtatcttc
    10501 taaattcccc ataaacattg gtttgtgctg gccagaaatc ctactgtggc aagatgctat
    10561 caactttttt atttgcagca tatgggttat gctttttgct ttatttttcc ctgcaagttt
    10621 tatccaaagc actaaatttg attaaaatta ggagtatttt ttaaaggaaa atagacacaa
    10681 aaagcaaaat tactaatatt aacccaaatt acttgctcaa tgggaatgct gtcaccagaa
    10741 ataataaaaa tatatatact actaaaattt cagtgaagaa ttagcaaaat tctttgtact
    10801 cagctcaaaa tcaaagaaag aaaaaagaaa aatctgtaac tcacaaaact taagtttatc
    10861 acacaataat caagttccaa acctatgcaa ctttaaagag gtatatatta aaccataaaa
    10921 aattcaaaag ggaacacatg gaaagtatct attaagcaaa catgaggtgc cattagcaaa
    10981 atgaaaccat tttaaaggct ttaatttagc tatatagtag ctgtgcatgc tccttgcttt
    11041 caatgaagta ttttattttc tacagttgtt tatatagttt gcttacttgt cctggggaga
    11101 caaggattca tagaaaaaga gggtctgaag ggagaaatta ttggacaact tattattacc
    11161 tactgtgcct ggaatgtgtt gaaactacgt ggccttcccc atccatctct ctccctctcc
    11221 cctttccagg cttcactatc ccagtttaaa aggacaaatt ctgtcatcat ttctcagatt
    11281 ctgcctcctc tggcttttaa ttcccctcct gactctccag tacataccag gggtccttcc
    11341 ccagggccta cctgcttaga gtgcacaggt accaagtcaa aagcagaaac aaacgcacca
    11401 ttagcaataa caactaaagc cagaaggggg tatcagcaag tttagaggcc aaagagacac
    11461 tgtcctgatg aagaagaaaa catggttgga ctgcattttt tacctatgag taatcaaaaa
    11521 tgcagtttgg gcagaagtac tgtggtcagg tttggacctt caccctgcta attatgtact
    11581 ctgagctgca cataggctga gctgtactca tagacaaaat gcagtgcgtt taagtctcta
    11641 ccaagctcta aaacttgaaa ggaaatagct gggttgttga ggaatatggg accaaattca
    11701 ttaatatttc ccaagataac catctcaaat gcaaatcttt ctactaaata tgtgtgtagc
    11761 ctatgggtgg gtctgttttc aggcaatatg ccaaaaggaa gagaccaata catctttttc
    11821 attctctgta gtaacagaca ttttaatcag ggaaaactgg cattcgggta agttttcata
    11881 tagtttggga caaaattcta atagccatcc atagaaaacc ttaacaaaag atgctagggt
    11941 ggaaagaaag ccatacagaa aagctcatat ctattacaca tcttgttata agcaattcat
    12001 atttgtattg gacttttaat ttttatttta tgtaaaagca tctttagata accatgtgaa
    12061 ataaatatta cttattttat tataaatatg tagattttta aagactatta taatgtaaat
    12121 gagatggata tagaaaatgc caagcatact gctggacaat tagtaggtat gcaatttaaa
    12181 ataagaattc caaaggaagt gatttgagtc acacttgtgt ttgcaatact tagctcccaa
    12241 attcttctgt aactttgtat gtgtgtttaa tcaaaaccca ctgttacata aaatgtaagg
    12301 cattttattt gttaagttat atacatatag gtatcctatg ttgttttcaa gagggatatt
    12361 actttaacaa taacatgcta tgaaaactag tactacattt ttaacctaat tgccttaaat
    12421 ttttagtgtt cagtaggcga gtgtcaattt cttcttgagg aaaacagctt cctcaatgat
    12481 ttcaatttac tgtctgtttg gagagtctgt tatatctttc tgttgccatg aatttaaaat
    12541 tgcatcgtca ccttggaaac attcaatata gtagtccatt ttcccgcttt gaatattttc
    12601 aaaccacggt ttcagtaact ggccctttgc caactatttt gtgagtacaa gggctttggc
    12661 attaaacata ttttttctca ttaaatgttt ttgtcttaaa aatctaaagt ataatctgtc
    12721 atcagtacag attttcatca attttgccta ttctactgtt aaatcttact aatcaagaaa
    12781 ggtgattcct cgggggccta atttttcatg tcaatgtatt taagatattg ggttatagaa
    12841 ctaagatgaa cccccattat atagtgacaa tgcgtgatga ccaggggaca ccatacagga
    12901 acaacgccct gtacactcag ccctgacaca ctgcagccgc ttcagccaaa tcagactgac
    12961 cactgtagaa ccagactgtg gtaactctga ccctaaaatc aaactagccc tgaagttctt
    13021 ctattgaatc tacaaaagaa tttccaagat gcagtaaaac ttcattctca gaggatcaag
    13081 tagccatcac tggaaagctg aaaaccgtaa ttttaatgaa tttttgtttg tttaattggt
    13141 ttagtgtgat tattttggag ttggttcttt gtggtttgga gggaatggca gctctttgtg
    13201 ggggttgcct agtaatttta taaaaacttt agtataacac attacttatt ttggtctttc
    13261 aattcattat aatgcttaaa agaataattc atgttctaaa tcataactgc atgacgattt
    13321 agatcttctt actatttagt tatagcctaa tggatactgt attcctctgt tttctcaaaa
    13381 gaataaagtt cctagtttat taaatagtat tctgacagaa aactatgtgg cccaaccagc
    13441 tctgagtata ctgataccta tctctatgta gcttatacgc aacttccatg ttgactgtca
    13501 tgtgtctgct gcattctgaa gctgccactg tggccgtctc atcttcctat gccaacaccc
    13561 cagctgtggt gtcctgtggt gtcatgcaca taggctacgg tatgaacaca gctgtaatct
    13621 gcttattcaa catcaaatcc acaaagtctg aggcagcgca ataacactgt agtccaaatg
    13681 gcgcactgtg cacgtcatct gcatccctgc cttctttaac acatatgctg aagcagtgtt
    13741 ttaagtgttt tttcccaact tgtaataaac atctctgtgt catatctaca tagtcaagct
    13801 atgcctagta attaagtttt aaaatgttgc ctattatcta aagaaaaata tctactaaat
    13861 ttctcatata ttcactcaaa ttctcttctg ttatcaatat aggcctatct atagtatatg
    13921 taaaatataa gaagggtcct gtgatgagat tttgcctgcc aaactagcta ttgaaccagg
    13981 tcaaagattg tactgtcctc aggagttatt gatctccaac atactgggtg tgggtacact
    14041 tggtgttgaa gcacttgcct tgtgcatgca aggctgtggg atcaaacccc aacactagaa
    14101 aactaacaaa gttaggtatc tgtaaaatta atgataatag acattgagat tctagtattt
    14161 aggggaagtg ggagtaaagg ggaggggaaa tggaagaaga tggagggaaa aaacagccct
    14221 gataacaaag gaagatatta aatagaggag ttgatgcaca cagtatatgt taatggagaa
    14281 ataattggtg gagttaatac atatagtata tatttatgga gaagtgaccg gctttgggag
    14341 gtgtgcacat acaggccctc atgtgtttag aaggggatcc taaattatct gctgggattt
    14401 ccccccttgg tataccagtc ttttttattt taattgttta acttgcaaaa gttattaatt
    14461 caagatttaa taattataag cttattgtta aactcatgac aactctgtga aggttcgtat
    14521 tgattttctg aaaatttatg atgaaagtat ttagtactgt cagtaattaa tgcatacaaa
    14581 aagcctcatt gggggcaatc ctggatggtt tctctataca atatgttaat aaacatccca
    14641 ttctttttct ctcacattat aaagatttta aatgttttaa tgaagtccct aagcattccc
    14701 ccaggttaaa aaatactcaa aaatattttt ttaaatctaa ggagtaaagt atcagaaatc
    14761 aaatactaaa tgaggagttc tttacatgct tctagacata tatattcaat aaaccaactt
    14821 tgtatcttgg agctatacac atgattaggt aactacagtg catggtccat cattcccttc
    14881 agtgtcttag agggttttca tactagtcta taaagctctg aacgctgcag ggaagaagaa
    14941 gcacaacctg tattttaaga gtttccaaac aagtgatcct ctcctgtctt cccatgtaat
    15001 cacgctgtgc tctgtcctgt actctgttgg ctttcgatac agattcttgc caggagctaa
    15061 agcacacgca taaatggcta caccttgtct cactcacctg cctccacgtg ctttattcca
    15121 ctatatttgc aaacaagatg ttttgggttc agttctgtaa ccaggatgtg ttttagcata
    15181 tgcctgtttg tttgactgtc caacctgcac cattaataca atgcctttcc cccccatcta
    15241 atggtgtttg tatctgtgtc cctatatctg ctgcatttta aattcataac aagaaaagat
    15301 gattttgtat ttatactttg ctgatctagt cagtaattct gcaccaatca gcagacttct
    15361 ctgcatgtag tagtctgtac aattgttcaa catcagaata cttgttcatg tcaaagtgtc
    15421 tataaaattg ccaccattgt tcttcatttc agtctggtag aatacatgag acagaaaata
    15481 aatgtgtaaa tgac
//
